################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraIII Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI KspI MaeI MamI MfeI MroI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 1040 GTCGAC GT^[AC][GT]AC AflIII 1524 ACGCGT A^C[GA][CT]GT Alw44I 585 GTGCAC G^TGCAC AlwNI 979 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1177 CCTGAGG CC^T[ACGT]AGG ApaLI 585 GTGCAC G^TGCAC BalI 2098 TGGCCA TGG^CCA BanI 1518 GGCACC G^G[CT][GA]CC BbvCI 2131 GCTGAGG CCTCAGC(-5/-2) BcgI 991 CGAGTGTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2562 GGATAC GTATCC(6/5) BsaI 517 GGTCTC GGTCTC(1/5) BsgI 1279 GTGCAG GTGCAG(16/14) BsiEI 1145 CGGCCG CG[GA][CT]^CG BsmBI 1416 CGTCTC CGTCTC(1/5) BsmI 708 GAATGC GAATGC(1/-1) BsrBI 332 GAGCGG CCGCTC(-3/-3) BsrDI 402 GCAATG GCAATG(2/0) BssSI 580 CTCGTG CACGAG(-5/-1) Bsu36I 1177 CCTGAGG CC^T[ACGT]AGG CfoI 147 GCGC GCG^C DraII 782 AGGACCT [GA]G^G[ACGT]CC[CT] DrdI 2510 GACCAACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1035 CCATGG C^C[GA][CT]GG EagI 1145 CGGCCG C^GGCCG EclXI 1145 CGGCCG C^GGCCG Eco52I 1145 CGGCCG C^GGCCG Eco81I 1177 CCTGAGG CC^T[ACGT]AGG EcoO109I 782 AGGACCT [GA]G^G[ACGT]CC[CT] HhaI 147 GCGC GCG^C HinP1I 147 GCGC G^CGC HincII 1040 GTCGAC GT[CT]^[GA]AC HindII 1040 GTCGAC GT[CT]^[GA]AC HindIII 1241 AAGCTT A^AGCTT MluI 1524 ACGCGT A^CGCGT MluNI 2098 TGGCCA TGG^CCA MscI 2098 TGGCCA TGG^CCA MslI 610 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 512 CAGCTG C[AC]G^C[GT]G NaeI 1001 GCCGGC GCC^GGC NcoI 1035 CCATGG C^CATGG NgoMIV 1001 GCCGGC G^CCGGC PpuMI 782 AGGACCT [GA]G^G[AT]CC[CT] PvuII 512 CAGCTG CAG^CTG SacI 1400 GAGCTC GAGCT^C SalI 1040 GTCGAC G^TCGAC ScaI 1101 AGTACT AGT^ACT SexAI 591 ACCAGGT A^CC[AT]GGT Tsp45I 274 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 148 CGCG CG^CG 1525 CGCG Asp700I 2179 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2320 GAAGCCTTTC BbsI 1756 GAAGAC GAAGAC(2/6) 2500 GAAGAC BpuAI 1756 GAAGAC GAAGAC(2/6) 2500 GAAGAC BseRI 1882 GAGGAG GAGGAG(10/8) 2481 GAGGAG BsiYI 22 CCGGGTTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1148 CCGTGAATTGG BslI 22 CCGGGTTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1148 CCGTGAATTGG BspHI 501 TCATGA T^CATGA 828 TCATGA BspMI 138 GCAGGT ACCTGC(4/8) 1207 GCAGGT BsrFI 1001 GCCGGC [GA]^CCGG[CT] 2566 ACCGGC BstUI 148 CGCG CG^CG 1525 CGCG Cac8I 790 GCACGC GC[ACGT]^[ACGT]GC 1001 GCCGGC Cfr10I 1001 GCCGGC [GA]^CCGG[CT] 2566 ACCGGC DraI 1389 TTTAAA TTT^AAA 2044 TTTAAA EcoT14I 1035 CCATGG C^C[AT][AT]GG 1603 CCAAGG MvnI 148 CGCG CG^CG 1525 CGCG PleI 357 GACTC GAGTC(4/5) 1694 GACTC PstI 889 CTGCAG CTGCA^G 1097 CTGCAG RcaI 501 TCATGA T^CATGA 828 TCATGA SapI 942 GAAGAGC GCTCTTC(1/4) 2590 GAAGAGC SfcI 889 CTGCAG C^T[GA][CT]AG 1097 CTGCAG StyI 1035 CCATGG C^C[AT][AT]GG 1603 CCAAGG XcmI 1559 CCACCAAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1622 CCAATGACGAAGTGG XmnI 2179 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2320 GAAGCCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanII BbvI BcnI BmyI BsaJI BsiHKAI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cfr13I DdeI DpnII EaeI EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII