(Circular) (Six-base) MAPSORT of: pFN21AE2203.seq Check: 9758 from: 1 to: 7330 pFN21AE2203 7330 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5140 278 Size: 53 83 186 936 3604 2468 Fragments arranged by size: 3604 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3046 4673 1937 Size: 1109 1627 4594 Fragments arranged by size: 4594 1627 1109 Acc65I G'GTAC_C Cuts at: 4651 4651 Size: 7330 AclI AA'CG_TT Cuts at: 1563 5457 5830 1563 Size: 3894 373 3063 Fragments arranged by size: 3894 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2147 2960 3152 3281 3527 4175 4437 5393 Size: 515 813 192 129 246 648 262 956 Cuts at: 5393 6777 7045 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 956 813 648 515 268 262 246 192 129 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7133 AflIII A'CryG_T Cuts at: 1203 3529 6229 7206 1203 Size: 2326 2700 977 1327 Fragments arranged by size: 2700 2326 1327 977 AgeI A'CCGG_T Cuts at: 6161 6161 Size: 7330 AhdI GACnn_n'nnGTC Cuts at: 6059 6059 Size: 7330 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7330 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4137 1184 Size: 2953 4377 AlwNI CAG_nnn'CTG Cuts at: 2989 5073 6645 2989 Size: 2084 1572 3674 Fragments arranged by size: 3674 2084 1572 ApaLI G'TGCA_C Cuts at: 2590 5387 6543 2590 Size: 2797 1156 3377 Fragments arranged by size: 3377 2797 1156 ApoI r'AATT_y Cuts at: 1493 3644 4045 4205 4639 4865 5017 7126 1493 Size: 2151 401 160 434 226 152 2109 1697 Fragments arranged by size: 2151 2109 1697 434 401 226 160 152 AseI AT'TA_AT Cuts at: 160 1032 5884 160 Size: 872 4852 1606 Fragments arranged by size: 4852 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7330 AvaI C'yCGr_G Cuts at: 1870 1943 4655 1870 Size: 73 2712 4545 Fragments arranged by size: 4545 2712 73 BamHI G'GATC_C Cuts at: 4660 5027 4660 Size: 367 6963 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3523 4651 6106 618 Size: 325 217 627 1736 1128 1455 1842 Fragments arranged by size: 1842 1736 1455 1128 627 325 217 BanII G_rGCy'C Cuts at: 1391 2041 3409 3616 4649 1391 Size: 650 1368 207 1033 4072 Fragments arranged by size: 4072 1368 1033 650 207 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3768 4512 928 Size: 971 1869 744 3746 Fragments arranged by size: 3746 1869 971 744 BbvCI CC'TCA_GC Cuts at: 4137 4137 Size: 7330 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2985 3019 5521 5555 1903 Size: 34 1048 34 2502 34 3678 Fragments arranged by size: 3678 2502 1048 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4561 5222 6432 4561 Size: 661 1210 5459 Fragments arranged by size: 5459 1210 661 BclI T'GATC_A Cuts at: 1548 1548 Size: 7330 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5941 136 Size: 107 122 71 631 605 4269 1525 Fragments arranged by size: 4269 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7325 7325 Size: 7330 BlpI GC'TnA_GC Cuts at: 4747 4747 Size: 7330 Bme1580I G_kGCm'C Cuts at: 1790 2594 5391 6547 1790 Size: 804 2797 1156 2573 Fragments arranged by size: 2797 2573 1156 804 BmgBI CAC'GTC Cuts at: 1318 4510 1318 Size: 3192 4138 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5061 6019 442 Size: 416 53 83 399 24 3644 958 1753 Fragments arranged by size: 3644 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7330 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5990 1239 Size: 4751 2579 Bpu10I CC'TnA_GC Cuts at: 4137 4137 Size: 7330 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2821 3366 3795 5455 6320 6618 2821 Size: 545 429 1660 865 298 3533 Fragments arranged by size: 3533 1660 865 545 429 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2528 5993 882 Size: 617 1029 3465 2219 Fragments arranged by size: 3465 2219 1029 617 BsaAI yAC'GTr Cuts at: 493 1204 5121 493 Size: 711 3917 2702 Fragments arranged by size: 3917 2702 711 BsaBI GATnn'nnATC Cuts at: 7324 7324 Size: 7330 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5137 5519 6154 275 Size: 53 83 186 936 3604 382 635 1451 Fragments arranged by size: 3604 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5763 6161 6435 6582 6993 5763 Size: 398 274 147 411 6100 Fragments arranged by size: 6100 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4480 4510 1178 Size: 30 165 30 3077 30 3998 Fragments arranged by size: 3998 3077 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3902 4501 1186 Size: 2716 599 4015 Fragments arranged by size: 4015 2716 599 BseYI C'CCAG_C Cuts at: 2428 6533 7029 2428 Size: 4105 496 2729 Fragments arranged by size: 4105 2729 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3305 1163 Size: 573 1569 5188 Fragments arranged by size: 5188 1569 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3153 4780 5541 6569 1540 Size: 275 186 1152 1627 761 1028 2301 Fragments arranged by size: 2301 1627 1152 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2594 3409 3717 5391 5476 6547 2594 Size: 815 308 1674 85 1071 3377 Fragments arranged by size: 3377 1674 1071 815 308 85 BsmI GAATG_Cn' Cuts at: 2719 4841 4934 2719 Size: 2122 93 5115 Fragments arranged by size: 5115 2122 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3427 3427 Size: 7330 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2594 3409 3616 3717 4649 5391 Size: 399 251 553 815 207 101 932 742 Cuts at: 5391 5476 6547 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 932 815 742 553 399 251 207 101 85 BspEI T'CCGG_A Cuts at: 6993 6993 Size: 7330 BspHI T'CATG_A Cuts at: 2506 2833 5219 6949 2506 Size: 327 2386 1730 2887 Fragments arranged by size: 2887 2386 1730 327 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2134 3203 4685 844 Size: 1290 1069 1482 3489 Fragments arranged by size: 3489 1482 1290 1069 BsrBI CCG'CTC Cuts at: 1394 1823 2339 5217 1394 Size: 429 516 2878 3507 Fragments arranged by size: 3507 2878 516 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2414 5825 5999 65 Size: 1183 1166 3411 174 1396 Fragments arranged by size: 3411 1396 1183 1166 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3006 4571 5974 6161 1700 Size: 110 1196 1565 1403 187 2869 Fragments arranged by size: 2869 1565 1403 1196 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7330 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7330 BssSI C'ACGA_G Cuts at: 2585 5390 6402 2585 Size: 2805 1012 3513 Fragments arranged by size: 3513 2805 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7330 BstBI TT'CG_AA Cuts at: 6134 6134 Size: 7330 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7330 BstXI CCAn_nnnn'nTGG Cuts at: 2308 3139 3574 2308 Size: 831 435 6064 Fragments arranged by size: 6064 831 435 BstYI r'GATC_y Cuts at: 1972 2351 2542 3806 4393 4660 5027 5412 5429 Size: 379 191 1264 587 267 367 385 17 Cuts at: 5429 6881 6967 6979 7325 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1264 587 385 379 367 346 267 191 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3183 3183 Size: 7330 BtgI C'CryG_G Cuts at: 513 1065 2004 3040 513 Size: 552 939 1036 4803 Fragments arranged by size: 4803 1036 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6591 Fragments arranged by size: 6591 683 56 BtsI GCAGTG_nn' Cuts at: 4106 4846 5640 5660 4106 Size: 740 794 20 5776 Fragments arranged by size: 5776 794 740 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7330 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7295 DraI TTT'AAA Cuts at: 3396 4051 4634 3396 Size: 655 583 6092 Fragments arranged by size: 6092 655 583 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4521 6337 809 Size: 507 3205 1816 1802 Fragments arranged by size: 3205 1816 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2340 3150 Size: 54 1425 50 275 18 186 324 810 Cuts at: 3150 4103 4777 5666 7295 8 Size: 953 674 889 1629 43 Fragments arranged by size: 1629 1425 953 889 810 674 324 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3150 4777 1537 Size: 275 1338 1627 4090 Fragments arranged by size: 4090 1627 1338 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2942 3777 3828 4034 4191 4276 4314 4478 Size: 1589 835 51 206 157 85 38 164 Cuts at: 4478 4590 5260 1353 Size: 112 670 3423 Fragments arranged by size: 3423 1589 835 670 206 164 157 112 85 51 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5917 6291 6437 387 Size: 1064 138 4328 374 146 1280 Fragments arranged by size: 4328 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3407 3407 Size: 7330 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2960 3152 3281 3527 4175 4437 Size: 393 515 813 192 129 246 648 262 Cuts at: 4437 5393 5990 6777 7045 1239 Size: 956 597 787 268 1524 Fragments arranged by size: 1524 956 813 787 648 597 515 393 268 262 246 192 129 EcoO109I rG'GnC_Cy Cuts at: 2788 2788 Size: 7330 EcoRI G'AATT_C Cuts at: 4639 5017 4639 Size: 378 6952 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2348 2348 Size: 7330 FspI TGC'GCA Cuts at: 5836 5836 Size: 7330 HaeII r_GCGC'y Cuts at: 1678 6477 1678 Size: 4799 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2050 2082 2920 2952 3656 Size: 32 135 32 478 32 838 32 704 Cuts at: 3656 3688 5971 6003 6045 6077 1373 Size: 32 2283 32 42 32 2626 Fragments arranged by size: 2626 2283 838 704 478 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3047 4674 4920 669 Size: 988 281 1109 1627 246 3079 Fragments arranged by size: 3079 1627 1109 988 281 246 HindIII A'AGCT_T Cuts at: 748 3246 748 Size: 2498 4832 HpaI GTT'AAC Cuts at: 4920 4920 Size: 7330 KpnI G_GTAC'C Cuts at: 4655 4655 Size: 7330 MfeI C'AATT_G Cuts at: 4929 4929 Size: 7330 MluI A'CGCG_T Cuts at: 3529 7206 3529 Size: 3677 3653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6444 6628 1305 Size: 5139 184 2007 Fragments arranged by size: 5139 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4105 10 Size: 54 1425 343 2273 3235 Fragments arranged by size: 3235 2273 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2619 5288 5647 5806 518 Size: 648 1453 2669 359 159 2042 Fragments arranged by size: 2669 2042 1453 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2519 4746 5423 6571 6816 1606 Size: 913 2227 677 1148 245 2120 Fragments arranged by size: 2227 2120 1148 913 677 245 NaeI GCC'GGC Cuts at: 3008 3008 Size: 7330 NcoI C'CATG_G Cuts at: 513 1065 2004 3040 513 Size: 552 939 1036 4803 Fragments arranged by size: 4803 1036 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7330 NgoMIV G'CCGG_C Cuts at: 3006 3006 Size: 7330 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7330 NotI GC'GGCC_GC Cuts at: 4777 4777 Size: 7330 NspI r_CATG'y Cuts at: 1132 1229 4688 6233 1132 Size: 97 3459 1545 2229 Fragments arranged by size: 3459 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7330 PciI A'CATG_T Cuts at: 6229 6229 Size: 7330 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7330 PmeI GTTT'AAAC Cuts at: 4634 4634 Size: 7330 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5399 5431 6912 6944 5399 Size: 32 1481 32 5785 Fragments arranged by size: 5785 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2788 2788 Size: 7330 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7330 PsiI TTA'TAA Cuts at: 4900 4900 Size: 7330 PstI C_TGCA'G Cuts at: 830 2898 3106 4682 830 Size: 2068 208 1576 3478 Fragments arranged by size: 3478 2068 1576 208 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7330 PvuII CAG'CTG Cuts at: 2519 2519 Size: 7330 SacI G_AGCT'C Cuts at: 3409 3409 Size: 7330 SalI G'TCGA_C Cuts at: 1936 3045 4672 1936 Size: 1109 1627 4594 Fragments arranged by size: 4594 1627 1109 SapI GCTCTTCn'nnn_ Cuts at: 2942 4590 2942 Size: 1648 5682 SbfI CC_TGCA'GG Cuts at: 4682 4682 Size: 7330 ScaI AGT'ACT Cuts at: 3108 5578 7290 3108 Size: 2470 1712 3148 Fragments arranged by size: 3148 2470 1712 SexAI A'CCwGG_T Cuts at: 2596 2596 Size: 7330 SfcI C'TryA_G Cuts at: 826 1045 2894 3102 4678 5813 6494 6685 826 Size: 219 1849 208 1576 1135 681 191 1471 Fragments arranged by size: 1849 1576 1471 1135 681 219 208 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7330 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7330 SmaI CCC'GGG Cuts at: 1872 4657 1872 Size: 2785 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2800 3381 3810 5434 6335 6597 Size: 197 926 857 581 429 1624 901 262 Cuts at: 6597 820 Size: 1553 Fragments arranged by size: 1624 1553 926 901 857 581 429 262 197 SnaBI TAC'GTA Cuts at: 493 5121 493 Size: 4628 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7330 SphI G_CATG'C Cuts at: 1132 4688 1132 Size: 3556 3774 SspI AAT'ATT Cuts at: 5 52 5254 5 Size: 47 5202 2081 Fragments arranged by size: 5202 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7330 StyI C'CwwG_G Cuts at: 513 1065 2004 3040 3608 4769 513 Size: 552 939 1036 568 1161 3074 Fragments arranged by size: 3074 1161 1036 939 568 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5357 5374 5527 5712 6157 325 Size: 1255 3777 17 153 185 445 1498 Fragments arranged by size: 3777 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2329 3106 5576 Size: 274 80 33 51 1443 352 777 2470 Cuts at: 5576 7288 96 Size: 1712 138 Fragments arranged by size: 2470 1712 1443 777 352 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4480 4512 7290 7322 4480 Size: 32 2778 32 4488 Fragments arranged by size: 4488 2778 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7330 XbaI T'CTAG_A Cuts at: 4666 4666 Size: 7330 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3571 3634 3571 Size: 63 7267 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7330 XmaI C'CCGG_G Cuts at: 1870 4655 1870 Size: 2785 4545 XmnI GAAnn'nnTTC Cuts at: 4188 4329 5459 4188 Size: 141 1130 6059 Fragments arranged by size: 6059 1130 141 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5138 276 Size: 53 83 186 936 3604 2468 Fragments arranged by size: 3604 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI