################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccII 5 CGCG CG^CG AlwNI 358 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 304 CCTCAGG CC^T[ACGT]AGG ApaI 53 GGGCCC GGGCC^C BanI 111 GGCACC G^G[CT][GA]CC BpmI 364 CTGGAG CTGGAG(16/14) BsgI 71 CTGCAC GTGCAG(16/14) BstAPI 178 GCAACCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 5 CGCG CG^CG Bsu36I 304 CCTCAGG CC^T[ACGT]AGG CfoI 145 GCGC GCG^C DpnII 351 GATC ^GATC DraII 53 GGGCCCC [GA]G^G[ACGT]CC[CT] EaeI 44 CGGCCA [CT]^GGCC[GA] EarI 132 CTCTTC CTCTTC(1/4) Eco81I 304 CCTCAGG CC^T[ACGT]AGG EcoO109I 53 GGGCCCC [GA]G^G[ACGT]CC[CT] FauI 29 CCCGC CCCGC(4/6) HhaI 145 GCGC GCG^C HinP1I 145 GCGC G^CGC HphI 385 GGTGA GGTGA(8/7) Ksp632I 132 CTCTTC CTCTTC(1/4) MboI 351 GATC ^GATC MboII 133 TCTTC GAAGA(8/7) MvnI 5 CGCG CG^CG NdeII 351 GATC ^GATC NlaIII 33 CATG CATG^ PvuII 188 CAGCTG CAG^CTG SapI 131 GCTCTTC GCTCTTC(1/4) Sau3AI 351 GATC ^GATC StuI 280 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BanII 53 GGGCCC G[GA]GC[CT]^C 204 GGGCTC BcnI 51 CCGGG CC^[GC]GG 149 CCCGG BlpI 94 GCTCAGC GC^T[ACGT]AGC 268 GCTGAGC Bpu1102I 94 GCTCAGC GC^T[ACGT]AGC 268 GCTGAGC BsiYI 236 CCATTACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 375 CCAGAACCAGG BslI 236 CCATTACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 375 CCAGAACCAGG BsrI 241 ACTGG ACTGG(1/-1) 88 CCAGT CelII 94 GCTCAGC GC^T[ACGT]AGC 268 GCTGAGC MaeIII 230 GTCAC ^GT[ACGT]AC 386 GTGAC MspA1I 107 CAGCGG C[AC]G^C[GT]G 188 CAGCTG NciI 51 CCGGG CC^[GC]GG 149 CCCGG PstI 122 CTGCAG CTGCA^G 185 CTGCAG SfcI 122 CTGCAG C^T[GA][CT]AG 185 CTGCAG SfiI 36 GGCCCCTCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 45 GGCCAGCCGGGCC Tsp45I 230 GTCAC ^GT[GC]AC 386 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI AluI BbvI BglI BmyI BsaJI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII HapII HpaII ItaI MnlI MspI MvaI MwoI NlaIV Sau96I ScrFI TseI TspRI