(Circular) (Six-base) MAPSORT of: pFN21AE2414.seq Check: 4693 from: 1 to: 5158 pFN21AE2414 5158 bp With 182 enzymes: SgfI * June 21, 2012 13:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2968 278 Size: 53 83 186 936 1432 2468 Fragments arranged by size: 2468 1432 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2501 1937 Size: 564 4594 Acc65I G'GTAC_C Cuts at: 2479 2479 Size: 5158 AclI AA'CG_TT Cuts at: 1563 3285 3658 1563 Size: 1722 373 3063 Fragments arranged by size: 3063 1722 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2239 3221 4605 4873 1632 Size: 607 982 1384 268 1917 Fragments arranged by size: 1917 1384 982 607 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4961 AflIII A'CryG_T Cuts at: 1203 4057 5034 1203 Size: 2854 977 1327 Fragments arranged by size: 2854 1327 977 AgeI A'CCGG_T Cuts at: 3989 3989 Size: 5158 AhdI GACnn_n'nnGTC Cuts at: 3887 3887 Size: 5158 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5158 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5158 AlwNI CAG_nnn'CTG Cuts at: 2368 2901 4473 2368 Size: 533 1572 3053 Fragments arranged by size: 3053 1572 533 ApaI G_GGCC'C Cuts at: 2062 2062 Size: 5158 ApaLI G'TGCA_C Cuts at: 3215 4371 3215 Size: 1156 4002 ApoI r'AATT_y Cuts at: 1493 2467 2693 2845 4954 1493 Size: 974 226 152 2109 1697 Fragments arranged by size: 2109 1697 974 226 152 AseI AT'TA_AT Cuts at: 160 1032 3712 160 Size: 872 2680 1606 Fragments arranged by size: 2680 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5158 AvaI C'yCGr_G Cuts at: 1870 1943 2483 1870 Size: 73 540 4545 Fragments arranged by size: 4545 540 73 BamHI G'GATC_C Cuts at: 2488 2855 2488 Size: 367 4791 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2116 2479 3934 618 Size: 325 217 627 329 363 1455 1842 Fragments arranged by size: 1842 1455 627 363 329 325 217 BanII G_rGCy'C Cuts at: 1391 2062 2213 2477 1391 Size: 671 151 264 4072 Fragments arranged by size: 4072 671 264 151 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3349 3383 1903 Size: 34 50 34 1328 34 3678 Fragments arranged by size: 3678 1328 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3050 4260 3050 Size: 1210 3948 BclI T'GATC_A Cuts at: 1548 1548 Size: 5158 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 2057 2180 Size: 107 122 71 631 605 376 9 123 Cuts at: 2180 2284 3769 136 Size: 104 1485 1525 Fragments arranged by size: 1525 1485 631 605 376 123 122 107 104 71 9 BglII A'GATC_T Cuts at: 5153 5153 Size: 5158 BlpI GC'TnA_GC Cuts at: 2100 2274 2575 2100 Size: 174 301 4683 Fragments arranged by size: 4683 301 174 Bme1580I G_kGCm'C Cuts at: 1790 2062 2226 2380 3219 4375 1790 Size: 272 164 154 839 1156 2573 Fragments arranged by size: 2573 1156 839 272 164 154 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5158 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2256 2889 3847 Size: 416 53 83 399 24 839 633 958 Cuts at: 3847 442 Size: 1753 Fragments arranged by size: 1753 958 839 633 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5158 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2390 3818 1239 Size: 1151 1428 2579 Fragments arranged by size: 2579 1428 1151 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3283 4148 4446 3283 Size: 865 298 3995 Fragments arranged by size: 3995 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3821 882 Size: 617 2322 2219 Fragments arranged by size: 2322 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2949 493 Size: 711 1745 2702 Fragments arranged by size: 2702 1745 711 BsaBI GATnn'nnATC Cuts at: 5152 5152 Size: 5158 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2965 3347 3982 275 Size: 53 83 186 936 1432 382 635 1451 Fragments arranged by size: 1451 1432 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3591 3989 4263 4410 4821 3591 Size: 398 274 147 411 3928 Fragments arranged by size: 3928 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4933 Fragments arranged by size: 4933 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5158 BseYI C'CCAG_C Cuts at: 2319 4361 4857 2319 Size: 2042 496 2620 Fragments arranged by size: 2620 2042 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2061 1163 Size: 573 325 4260 Fragments arranged by size: 4260 573 325 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2608 3369 4397 1540 Size: 275 186 607 761 1028 2301 Fragments arranged by size: 2301 1028 761 607 275 186 BsiHKAI G_wGCw'C Cuts at: 3219 3304 4375 3219 Size: 85 1071 4002 Fragments arranged by size: 4002 1071 85 BsmI GAATG_Cn' Cuts at: 2669 2762 2669 Size: 93 5065 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2213 2226 2380 2477 3219 3304 Size: 399 272 151 13 154 97 742 85 Cuts at: 3304 4375 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 272 154 151 97 85 13 BspEI T'CCGG_A Cuts at: 4821 4821 Size: 5158 BspHI T'CATG_A Cuts at: 3047 4777 3047 Size: 1730 3428 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2513 844 Size: 1669 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3045 1394 Size: 429 1222 3507 Fragments arranged by size: 3507 1222 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3653 3827 65 Size: 1183 2405 174 1396 Fragments arranged by size: 2405 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3802 3989 1700 Size: 110 1992 187 2869 Fragments arranged by size: 2869 1992 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5158 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5158 BssSI C'ACGA_G Cuts at: 3218 4230 3218 Size: 1012 4146 BstAPI GCAn_nnn'nTGC Cuts at: 794 2189 794 Size: 1395 3763 BstBI TT'CG_AA Cuts at: 3962 3962 Size: 5158 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5158 BstYI r'GATC_y Cuts at: 1972 2488 2855 3240 3257 4709 4795 4807 5153 Size: 516 367 385 17 1452 86 12 346 Cuts at: 5153 1972 Size: 1977 Fragments arranged by size: 1977 1452 516 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2310 2310 Size: 5158 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4606 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4419 Fragments arranged by size: 4419 683 56 BtsI GCAGTG_nn' Cuts at: 2187 2674 3468 3488 2187 Size: 487 794 20 3857 Fragments arranged by size: 3857 794 487 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5158 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5123 DraI TTT'AAA Cuts at: 2462 2462 Size: 5158 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4165 809 Size: 507 2849 1802 Fragments arranged by size: 2849 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2454 2605 Size: 54 1425 50 275 18 219 405 151 Cuts at: 2605 3494 5123 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 405 275 219 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2605 1537 Size: 275 793 4090 Fragments arranged by size: 4090 793 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2143 3088 1353 Size: 790 945 3423 Fragments arranged by size: 3423 945 790 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3745 4119 4265 387 Size: 1064 138 2156 374 146 1280 Fragments arranged by size: 2156 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2239 2390 3221 3818 4605 4873 1239 Size: 393 607 151 831 597 787 268 1524 Fragments arranged by size: 1524 831 787 607 597 393 268 151 EcoO109I rG'GnC_Cy Cuts at: 2059 2059 Size: 5158 EcoRI G'AATT_C Cuts at: 2467 2845 2467 Size: 378 4780 FspI TGC'GCA Cuts at: 3664 3664 Size: 5158 HaeII r_GCGC'y Cuts at: 1678 4305 1678 Size: 2627 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3799 3831 3873 3905 1373 Size: 32 135 32 2227 32 42 32 2626 Fragments arranged by size: 2626 2227 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2502 2748 669 Size: 988 281 564 246 3079 Fragments arranged by size: 3079 988 564 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5158 HpaI GTT'AAC Cuts at: 2748 2748 Size: 5158 KpnI G_GTAC'C Cuts at: 2483 2483 Size: 5158 MfeI C'AATT_G Cuts at: 2757 2757 Size: 5158 MluI A'CGCG_T Cuts at: 5034 5034 Size: 5158 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4272 4456 1305 Size: 2967 184 2007 Fragments arranged by size: 2967 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3336 Fragments arranged by size: 3336 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3116 3475 3634 518 Size: 648 1950 359 159 2042 Fragments arranged by size: 2042 1950 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2114 2195 2574 3251 4399 4644 1606 Size: 508 81 379 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 508 379 245 81 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4606 NdeI CA'TA_TG Cuts at: 387 387 Size: 5158 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5158 NotI GC'GGCC_GC Cuts at: 2605 2605 Size: 5158 NspI r_CATG'y Cuts at: 1132 1229 2516 4061 1132 Size: 97 1287 1545 2229 Fragments arranged by size: 2229 1545 1287 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5158 PciI A'CATG_T Cuts at: 4057 4057 Size: 5158 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5158 PmeI GTTT'AAAC Cuts at: 2462 2462 Size: 5158 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3227 3259 4740 4772 3227 Size: 32 1481 32 3613 Fragments arranged by size: 3613 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5158 PsiI TTA'TAA Cuts at: 2728 2728 Size: 5158 PspOMI G'GGCC_C Cuts at: 2058 2058 Size: 5158 PstI C_TGCA'G Cuts at: 830 2131 2194 2510 830 Size: 1301 63 316 3478 Fragments arranged by size: 3478 1301 316 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5158 PvuII CAG'CTG Cuts at: 2195 2195 Size: 5158 SalI G'TCGA_C Cuts at: 1936 2500 1936 Size: 564 4594 SapI GCTCTTCn'nnn_ Cuts at: 2143 2143 Size: 5158 SbfI CC_TGCA'GG Cuts at: 2510 2510 Size: 5158 ScaI AGT'ACT Cuts at: 3406 5118 3406 Size: 1712 3446 SfcI C'TryA_G Cuts at: 826 1045 2127 2190 2506 3641 4322 4513 826 Size: 219 1082 63 316 1135 681 191 1471 Fragments arranged by size: 1471 1135 1082 681 316 219 191 63 SfiI GGCCn_nnn'nGGCC Cuts at: 2048 2057 2048 Size: 9 5149 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5158 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5158 SmaI CCC'GGG Cuts at: 1872 2485 1872 Size: 613 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3262 4163 4425 820 Size: 197 926 1319 901 262 1553 Fragments arranged by size: 1553 1319 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2949 493 Size: 2456 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5158 SphI G_CATG'C Cuts at: 1132 2516 1132 Size: 1384 3774 SspI AAT'ATT Cuts at: 5 52 3082 5 Size: 47 3030 2081 Fragments arranged by size: 3030 2081 47 StuI AGG'CCT Cuts at: 1517 2287 1517 Size: 770 4388 StyI C'CwwG_G Cuts at: 513 1065 2597 513 Size: 552 1532 3074 Fragments arranged by size: 3074 1532 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2095 3185 3202 3355 3540 3985 325 Size: 1255 515 1090 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1090 515 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3404 5116 96 Size: 274 80 33 51 1443 1427 1712 138 Fragments arranged by size: 1712 1443 1427 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5118 5150 5118 Size: 32 5126 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5158 XbaI T'CTAG_A Cuts at: 2494 2494 Size: 5158 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5158 XmaI C'CCGG_G Cuts at: 1870 2483 1870 Size: 613 4545 XmnI GAAnn'nnTTC Cuts at: 3287 3287 Size: 5158 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2966 276 Size: 53 83 186 936 1432 2468 Fragments arranged by size: 2468 1432 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI XcmI