################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AspI AviII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BsaI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 55 TCCGGA T^CCGGA AcsI 920 AAATTT [GA]^AATT[CT] AcyI 138 GGCGTC G[GA]^CG[CT]C AflIII 1489 ACATGT A^C[GA][CT]GT AlwNI 432 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 332 GGGCCC GGGCC^C ApoI 920 AAATTT [GA]^AATT[CT] Asp700I 1161 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 329 CCCGGG C^[CT]CG[GA]G AvrII 1683 CCTAGG C^CTAGG BalI 1989 TGGCCA TGG^CCA BlnI 1683 CCTAGG C^CTAGG BsaAI 709 CACGTA [CT]AC^GT[GA] BsaBI 1302 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 138 GGCGTC G[GA]^CG[CT]C BsaWI 55 TCCGGA [AT]^CCGG[AT] BseAI 55 TCCGGA T^CCGGA BsmBI 147 GAGACG CGTCTC(1/5) BsoBI 329 CCCGGG C^[CT]CG[GA]G BspEI 55 TCCGGA T^CCGGA BspLU11I 1489 ACATGT A^CATGT BsrBI 336 CCGCTC CCGCTC(-3/-3) BstAPI 1709 GCAATAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 502 GGTGACC G^GT[ACGT]ACC BstPI 502 GGTGACC G^GT[ACGT]ACC DraI 601 TTTAAA TTT^AAA EaeI 1989 TGGCCA [CT]^GGCC[GA] EcoO65I 502 GGTGACC G^GT[ACGT]ACC EcoT22I 699 ATGCAT ATGCA^T HgaI 139 GCGTC GACGC(5/10) Hin1I 138 GGCGTC G[GA]^CG[CT]C KspI 424 CCGCGG CCGC^GG MamI 1302 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1989 TGGCCA TGG^CCA MroI 55 TCCGGA T^CCGGA MscI 1989 TGGCCA TGG^CCA MslI 790 CACAAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1971 CCATGG C^CATGG NdeI 724 CATATG CA^TATG NsiI 699 ATGCAT ATGCA^T PflMI 431 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 2034 CAGCTG CAG^CTG SacII 424 CCGCGG CCGC^GG SmaI 329 CCCGGG CCC^GGG SmlI 1786 CTCAAG C^T[CT][GA]AG SspI 1368 AATATT AAT^ATT Van91I 431 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2121 TCTAGA T^CTAGA XcmI 1208 CCAGCTTGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 329 CCCGGG C^CCGGG XmaI 329 CCCGGG C^CCGGG XmnI 1161 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AocI 79 CCTGAGG CC^T[ACGT]AGG 630 CCTGAGG AseI 1825 ATTAAT AT^TAAT 1963 ATTAAT AsnI 1825 ATTAAT AT^TAAT 1963 ATTAAT AspHI 247 GTGCTC G[AT]GC[AT]^C 1678 GAGCAC BsiHKAI 247 GTGCTC G[AT]GC[AT]^C 1678 GAGCAC Bsu36I 79 CCTGAGG CC^T[ACGT]AGG 630 CCTGAGG DsaI 424 CCGCGG C^C[GA][CT]GG 1971 CCATGG Eco81I 79 CCTGAGG CC^T[ACGT]AGG 630 CCTGAGG PpuMI 570 GGGTCCC [GA]G^G[AT]CC[CT] 664 GGGACCC PshBI 1825 ATTAAT AT^TAAT 1963 ATTAAT StuI 475 AGGCCT AGG^CCT 1080 AGGCCT TfiI 1576 GATTC G^A[AT]TC 1634 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI AvaII BamHI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII