Restriction map for
pFN21AE5726
(Linear) (Six-base) MAPSORT of: KIEE5726.seq Check: 5123 from: 1 to: 2133
KIEE5726.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AccI GT'mk_AC
Cuts at: 0 827 1656 1735 2133
Size: 827 829 79 398
Fragments arranged by size:
829 827 398 79
AflIII A'CryG_T
Cuts at: 0 1489 2133
Size: 1489 644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 714 1361 2133
Size: 714 647 772
Fragments arranged by size:
772 714 647
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2014 2046 2133
Size: 2014 32 87
Fragments arranged by size:
2014 87 32
AlwNI CAG_nnn'CTG
Cuts at: 0 437 2133
Size: 437 1696
ApaI G_GGCC'C
Cuts at: 0 336 2133
Size: 336 1797
ApoI r'AATT_y
Cuts at: 0 920 2133
Size: 920 1213
AseI AT'TA_AT
Cuts at: 0 1826 1964 2133
Size: 1826 138 169
Fragments arranged by size:
1826 169 138
AvaI C'yCGr_G
Cuts at: 0 329 2133
Size: 329 1804
AvrII C'CTAG_G
Cuts at: 0 1683 2133
Size: 1683 450
BamHI G'GATC_C
Cuts at: 0 445 522 1307 2133
Size: 445 77 785 826
Fragments arranged by size:
826 785 445 77
BanII G_rGCy'C
Cuts at: 0 197 336 359 2133
Size: 197 139 23 1774
Fragments arranged by size:
1774 197 139 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 995 1226 2133
Size: 134 861 231 907
Fragments arranged by size:
907 861 231 134
BfrBI ATG'CAT
Cuts at: 0 701 2133
Size: 701 1432
Bme1580I G_kGCm'C
Cuts at: 0 336 1931 2133
Size: 336 1595 202
Fragments arranged by size:
1595 336 202
BmgBI CAC'GTC
Cuts at: 0 151 218 1377 2133
Size: 151 67 1159 756
Fragments arranged by size:
1159 756 151 67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1286 2133
Size: 1286 847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 362 571 2133
Size: 193 169 209 1562
Fragments arranged by size:
1562 209 193 169
Bpu10I CC'TnA_GC
Cuts at: 0 534 2133
Size: 534 1599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1771 2133
Size: 1771 362
BsaAI yAC'GTr
Cuts at: 0 711 2133
Size: 711 1422
BsaBI GATnn'nnATC
Cuts at: 0 1306 2133
Size: 1306 827
BsaHI Gr'CG_yC
Cuts at: 0 139 2133
Size: 139 1994
BsaWI w'CCGG_w
Cuts at: 0 55 2133
Size: 55 2078
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 544 574 2133
Size: 265 30 249 30 1559
Fragments arranged by size:
1559 265 249 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 83 97 127 175 220 273 1262 2133
Size: 83 14 30 48 45 53 989 871
Fragments arranged by size:
989 871 83 53 48 45 30 14
BseYI C'CCAG_C
Cuts at: 0 323 1207 1532 2133
Size: 323 884 325 601
Fragments arranged by size:
884 601 325 323
BsiHKAI G_wGCw'C
Cuts at: 0 251 1682 2133
Size: 251 1431 451
Fragments arranged by size:
1431 451 251
BsmBI CGTCTCn'nnnn_
Cuts at: 0 141 2133
Size: 141 1992
Bsp1286I G_dGCh'C
Cuts at: 0 197 251 336 359 1682 1931 2133
Size: 197 54 85 23 1323 249 202
Fragments arranged by size:
1323 249 202 197 85 54 23
BspEI T'CCGG_A
Cuts at: 0 55 2133
Size: 55 2078
BsrBI CCG'CTC
Cuts at: 0 338 2133
Size: 338 1795
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1715 2133
Size: 1715 418
BstEII G'GTnAC_C
Cuts at: 0 502 2133
Size: 502 1631
BstYI r'GATC_y
Cuts at: 0 50 445 457 522 1307 2133
Size: 50 395 12 65 785 826
Fragments arranged by size:
826 785 395 65 50 12
Bsu36I CC'TnA_GG
Cuts at: 0 80 631 2133
Size: 80 551 1502
Fragments arranged by size:
1502 551 80
BtgI C'CryG_G
Cuts at: 0 424 1971 2133
Size: 424 1547 162
Fragments arranged by size:
1547 424 162
BtsI GCAGTG_nn'
Cuts at: 0 1698 1964 2133
Size: 1698 266 169
Fragments arranged by size:
1698 266 169
DraI TTT'AAA
Cuts at: 0 603 2133
Size: 603 1530
EaeI y'GGCC_r
Cuts at: 0 1989 2133
Size: 1989 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 362 571 2133
Size: 193 169 209 1562
Fragments arranged by size:
1562 209 193 169
EcoO109I rG'GnC_Cy
Cuts at: 0 434 571 665 2133
Size: 434 137 94 1468
Fragments arranged by size:
1468 434 137 94
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1965 2133
Size: 1965 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 982 1014 1079 1111 2133
Size: 982 32 65 32 1022
Fragments arranged by size:
1022 982 65 32 32
MscI TGG'CCA
Cuts at: 0 1991 2133
Size: 1991 142
MslI CAynn'nnrTG
Cuts at: 0 794 2133
Size: 794 1339
MspA1I CmG'CkG
Cuts at: 0 47 230 426 2036 2133
Size: 47 183 196 1610 97
Fragments arranged by size:
1610 196 183 97 47
NcoI C'CATG_G
Cuts at: 0 1971 2133
Size: 1971 162
NdeI CA'TA_TG
Cuts at: 0 725 2133
Size: 725 1408
NsiI A_TGCA'T
Cuts at: 0 703 2133
Size: 703 1430
NspI r_CATG'y
Cuts at: 0 799 1493 1804 2133
Size: 799 694 311 329
Fragments arranged by size:
799 694 329 311
PasI CC'CwG_GG
Cuts at: 0 431 2133
Size: 431 1702
PciI A'CATG_T
Cuts at: 0 1489 2133
Size: 1489 644
PflMI CCAn_nnn'nTGG
Cuts at: 0 437 2133
Size: 437 1696
PpuMI rG'GwC_Cy
Cuts at: 0 571 665 2133
Size: 571 94 1468
Fragments arranged by size:
1468 571 94
PspOMI G'GGCC_C
Cuts at: 0 332 2133
Size: 332 1801
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2086 2118 2133
Size: 2086 32 15
Fragments arranged by size:
2086 32 15
PvuII CAG'CTG
Cuts at: 0 2036 2133
Size: 2036 97
SacII CC_GC'GG
Cuts at: 0 427 2133
Size: 427 1706
SanDI GG'GwC_CC
Cuts at: 0 571 665 2133
Size: 571 94 1468
Fragments arranged by size:
1468 571 94
SfcI C'TryA_G
Cuts at: 0 824 1076 1657 2082 2133
Size: 824 252 581 425 51
Fragments arranged by size:
824 581 425 252 51
SmaI CCC'GGG
Cuts at: 0 331 2133
Size: 331 1802
SmlI C'TyrA_G
Cuts at: 0 1786 2133
Size: 1786 347
SspI AAT'ATT
Cuts at: 0 1370 2133
Size: 1370 763
StuI AGG'CCT
Cuts at: 0 477 1082 2133
Size: 477 605 1051
Fragments arranged by size:
1051 605 477
StyI C'CwwG_G
Cuts at: 0 1683 1847 1971 2057 2133
Size: 1683 164 124 86 76
Fragments arranged by size:
1683 164 124 86 76
TatI w'GTAC_w
Cuts at: 0 1253 2133
Size: 1253 880
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 2133
Size: 265 32 1836
Fragments arranged by size:
1836 265 32
XbaI T'CTAG_A
Cuts at: 0 2121 2133
Size: 2121 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1215 2133
Size: 1215 918
XmaI C'CCGG_G
Cuts at: 0 329 2133
Size: 329 1804
XmnI GAAnn'nnTTC
Cuts at: 0 1165 2133
Size: 1165 968
Enzymes that do cut:
AccI AflIII AlfI AloI AlwNI ApaI ApoI AseI
AvaI AvrII BamHI BanII BbsI BfrBI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspEI BsrBI
BstAPI BstEII BstYI Bsu36I BtgI BtsI DraI EaeI
Eco57MI EcoO109I FalI Hin4I MscI MslI MspA1I NcoI
NdeI NsiI NspI PasI PciI PflMI PpuMI PspOMI
PsrI PvuII SacII SanDI SfcI SmaI SmlI SspI
StuI StyI TatI TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI ApaLI AscI AsiSI BaeI BanI BbeI
BbvCI BcgI BciVI BclI BglI BglII BlpI BmrI
BmtI BsaI BsgI BsiEI BsiWI BsmI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I
BtgZI ClaI CspCI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NgoMIV NheI NotI NruI PacI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI
RsrII SacI SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
TaqII Tth111I XhoI ZraI