################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII AccII AclI AflII Aor51HI ApaI AscI AseI AsnI AspI BalI BbrPI BfrI BsaAI BsiEI BsiWI BsmI BsrBI BssHII BstEII BstPI BstUI CpoI EaeI EagI EclXI Eco47III Eco52I EcoO65I EcoRI FseI KspI MaeII MluI MluNI MscI MvnI NaeI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 2650 GTATAC GT^[AC][GT]AC AccIII 2699 TCCGGA T^CCGGA AgeI 4157 ACCGGT A^CCGGT AviII 4673 TGCGCA TGC^GCA AvrII 1462 CCTAGG C^CTAGG BamHI 57 GGATCC G^GATCC BbvCI 2422 CCTCAGC CCTCAGC(-5/-2) BcgI 397 GCAGCAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1173 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1462 CCTAGG C^CTAGG BseAI 2699 TCCGGA T^CCGGA BsmBI 3284 CGTCTC CGTCTC(1/5) Bsp1407I 4522 TGTACA T^GTACA BspDI 4306 ATCGAT AT^CGAT BspEI 2699 TCCGGA T^CCGGA BsrDI 2852 GCAATG GCAATG(2/0) BsrFI 4157 ACCGGT [GA]^CCGG[CT] BsrGI 4522 TGTACA T^GTACA BssSI 1102 CACGAG CACGAG(-5/-1) Bst1107I 2650 GTATAC GTA^TAC BstAPI 1127 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3197 TTCGAA TT^CGAA BstZ17I 2650 GTATAC GTA^TAC Cfr10I 4157 ACCGGT [GA]^CCGG[CT] ClaI 4306 ATCGAT AT^CGAT DraIII 1786 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 25 GACAGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 3081 CCATGG C^C[GA][CT]GG FspI 4673 TGCGCA TGC^GCA HindIII 2140 AAGCTT A^AGCTT MroI 2699 TCCGGA T^CCGGA NcoI 3081 CCATGG C^CATGG NspV 3197 TTCGAA TT^CGAA PinAI 4157 ACCGGT A^CCGGT PshAI 3278 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 296 AGTACT AGT^ACT SfuI 3197 TTCGAA TT^CGAA SmaI 4640 CCCGGG CCC^GGG SmlI 4198 CTCAAG C^T[CT][GA]AG SspBI 4522 TGTACA T^GTACA SspI 69 AATATT AAT^ATT StuI 3332 AGGCCT AGG^CCT XcmI 2607 CCATGTTCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 4640 CCCGGG C^CCGGG XmaI 4640 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 1545 GGTACC G^GTACC 1575 GGTACC AcsI 888 AAATTT [GA]^AATT[CT] 1555 GAATTT AcyI 2177 GGCGCC G[GA]^CG[CT]C 2958 GGCGCC AhdI 3255 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4093 GACTACATGTC Alw44I 1271 GTGCAC G^TGCAC 4635 GTGCAC ApaLI 1271 GTGCAC G^TGCAC 4635 GTGCAC ApoI 888 AAATTT [GA]^AATT[CT] 1555 GAATTT Asp718I 1545 GGTACC G^GTACC 1575 GGTACC AspEI 3255 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4093 GACTACATGTC AvaI 1328 CTCGGG C^[CT]CG[GA]G 4640 CCCGGG BbeI 2177 GGCGCC GGCGC^C 2958 GGCGCC BciVI 1812 GGATAC GTATCC(6/5) 1489 GTATCC BglII 2359 AGATCT A^GATCT 3632 AGATCT BlpI 491 GCTTAGC GC^T[ACGT]AGC 4283 GCTCAGC BpmI 18 CTGGAG CTGGAG(16/14) 1648 CTGGAG Bpu1102I 491 GCTTAGC GC^T[ACGT]AGC 4283 GCTCAGC BsaBI 3786 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4291 GATGGATATC BsaHI 2177 GGCGCC G[GA]^CG[CT]C 2958 GGCGCC BsaWI 2699 TCCGGA [AT]^CCGG[AT] 4157 ACCGGT BseRI 764 CTCCTC GAGGAG(10/8) 3310 GAGGAG BsoBI 1328 CTCGGG C^[CT]CG[GA]G 4640 CCCGGG BstXI 740 CCATTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4580 CCAGGGTTCTGG CelII 491 GCTTAGC GC^T[ACGT]AGC 4283 GCTCAGC DraI 444 TTTAAA TTT^AAA 2623 TTTAAA Eam1105I 3255 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4093 GACTACATGTC EcoRV 2197 GATATC GAT^ATC 4295 GATATC EcoT22I 4016 ATGCAT ATGCA^T 4062 ATGCAT HaeII 2177 GGCGCC [GA]GCGC^[CT] 2958 GGCGCC HgaI 692 GACGC GACGC(5/10) 3283 GCGTC Hin1I 2177 GGCGCC G[GA]^CG[CT]C 2958 GGCGCC KasI 2177 GGCGCC G^GCGCC 2958 GGCGCC KpnI 1545 GGTACC GGTAC^C 1575 GGTACC MamI 3786 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4291 GATGGATATC MfeI 1895 CAATTG C^AATTG 3986 CAATTG MunI 1895 CAATTG C^AATTG 3986 CAATTG NarI 2177 GGCGCC GG^CGCC 2958 GGCGCC NsiI 4016 ATGCAT ATGCA^T 4062 ATGCAT PstI 1255 CTGCAG CTGCA^G 1471 CTGCAG PvuII 152 CAGCTG CAG^CTG 4142 CAGCTG SacI 4177 GAGCTC GAGCT^C 4723 GAGCTC SapI 1195 GAAGAGC GCTCTTC(1/4) 4720 GAAGAGC SfoI 2177 GGCGCC GGC^GCC 2958 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AlwNI AocI Asp700I AspHI AvaII BanI BanII BbsI BbvI BclI BcnI BfaI BmyI BpuAI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspLU11I BspMI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI RcaI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI