(Circular) (Six-base) MAPSORT of: pFN21AE3502.seq Check: 8832 from: 1 to: 9451 pFN21AE3502 9451 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2064 2064 Size: 9451 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7261 278 Size: 53 83 186 936 5725 2468 Fragments arranged by size: 5725 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4656 6794 1937 Size: 2719 2138 4594 Fragments arranged by size: 4594 2719 2138 Acc65I G'GTAC_C Cuts at: 3550 3580 6772 3550 Size: 30 3192 6229 Fragments arranged by size: 6229 3192 30 AclI AA'CG_TT Cuts at: 1563 7578 7951 1563 Size: 6015 373 3063 Fragments arranged by size: 6015 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2474 2592 2778 3206 3873 4320 4767 5574 Size: 842 118 186 428 667 447 447 807 Cuts at: 5574 5921 5938 6111 6218 6268 6459 6626 7514 Size: 347 17 173 107 50 191 167 888 Cuts at: 7514 8898 9166 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 888 842 807 667 447 447 428 347 268 191 186 173 167 118 107 50 17 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9254 AflIII A'CryG_T Cuts at: 1203 2965 5610 6102 8350 9327 1203 Size: 1762 2645 492 2248 977 1327 Fragments arranged by size: 2645 2248 1762 1327 977 492 AgeI A'CCGG_T Cuts at: 6162 8282 6162 Size: 2120 7331 AhdI GACnn_n'nnGTC Cuts at: 5265 6103 8180 5265 Size: 838 2077 6536 Fragments arranged by size: 6536 2077 838 AleI CACnn'nnGTG Cuts at: 1166 5955 1166 Size: 4789 4662 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9451 AlwNI CAG_nnn'CTG Cuts at: 3268 3722 5363 5378 6089 6323 7194 8766 3268 Size: 454 1641 15 711 234 871 1572 3953 Fragments arranged by size: 3953 1641 1572 871 711 454 234 15 ApaLI G'TGCA_C Cuts at: 3276 6640 7508 8664 3276 Size: 3364 868 1156 4063 Fragments arranged by size: 4063 3364 1156 868 ApoI r'AATT_y Cuts at: 1493 2893 3560 6760 6986 7138 9247 1493 Size: 1400 667 3200 226 152 2109 1697 Fragments arranged by size: 3200 2109 1697 1400 667 226 152 AseI AT'TA_AT Cuts at: 160 1032 8005 160 Size: 872 6973 1606 Fragments arranged by size: 6973 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9451 AvaI C'yCGr_G Cuts at: 1870 1943 3333 6645 6776 1870 Size: 73 1390 3312 131 4545 Fragments arranged by size: 4545 3312 1390 131 73 AvrII C'CTAG_G Cuts at: 3467 3467 Size: 9451 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3810 3843 3810 Size: 33 9418 BamHI G'GATC_C Cuts at: 2062 6781 7148 2062 Size: 4719 367 4365 Fragments arranged by size: 4719 4365 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2370 2662 3307 3403 3463 Size: 325 217 627 583 292 645 96 60 Cuts at: 3463 3550 3580 3637 3667 3952 4182 4726 4963 Size: 87 30 57 30 285 230 544 237 Cuts at: 4963 6028 6772 8227 618 Size: 1065 744 1455 1842 Fragments arranged by size: 1842 1455 1065 744 645 627 583 544 325 292 285 237 230 217 96 87 60 57 30 30 BanII G_rGCy'C Cuts at: 1391 2767 5227 6186 6732 6770 1391 Size: 1376 2460 959 546 38 4072 Fragments arranged by size: 4072 2460 1376 959 546 38 BbeI G_GCGC'C Cuts at: 4186 4967 4186 Size: 781 8670 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2147 5752 5909 928 Size: 971 248 3605 157 4470 Fragments arranged by size: 4470 3605 971 248 157 BbvCI CC'TCA_GC Cuts at: 4428 4428 Size: 9451 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2391 2425 7642 7676 1903 Size: 34 454 34 5217 34 3678 Fragments arranged by size: 5217 3678 454 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3505 3811 7343 8553 3505 Size: 306 3532 1210 4403 Fragments arranged by size: 4403 3532 1210 306 BclI T'GATC_A Cuts at: 1548 4588 4843 4882 5380 6073 6223 1548 Size: 3040 255 39 498 693 150 4776 Fragments arranged by size: 4776 3040 693 498 255 150 39 BfrBI ATG'CAT Cuts at: 6023 6069 6023 Size: 46 9405 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3184 8062 136 Size: 107 122 71 631 605 1512 4878 1525 Fragments arranged by size: 4878 1525 1512 631 605 122 107 71 BglII A'GATC_T Cuts at: 4364 5637 9446 4364 Size: 1273 3809 4369 Fragments arranged by size: 4369 3809 1273 BlpI GC'TnA_GC Cuts at: 2497 6289 6868 2497 Size: 3792 579 5080 Fragments arranged by size: 5080 3792 579 Bme1580I G_kGCm'C Cuts at: 1790 2605 3280 3466 3955 6031 6644 7512 8668 Size: 815 675 186 489 2076 613 868 1156 Cuts at: 8668 1790 Size: 2573 Fragments arranged by size: 2573 2076 1156 868 815 675 613 489 186 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9451 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 7182 8140 442 Size: 416 53 83 399 24 5765 958 1753 Fragments arranged by size: 5765 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9451 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2044 3674 8111 1239 Size: 805 1630 4437 2579 Fragments arranged by size: 4437 2579 1630 805 Bpu10I CC'TnA_GC Cuts at: 3282 3303 3357 4428 5541 3282 Size: 21 54 1071 1113 7192 Fragments arranged by size: 7192 1113 1071 54 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6188 7576 8441 8739 6188 Size: 1388 865 298 6900 Fragments arranged by size: 6900 1388 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4129 4440 4563 5275 6166 8114 882 Size: 617 2630 311 123 712 891 1948 2219 Fragments arranged by size: 2630 2219 1948 891 712 617 311 123 BsaAI yAC'GTr Cuts at: 493 1204 7242 493 Size: 711 6038 2702 Fragments arranged by size: 6038 2702 711 BsaBI GATnn'nnATC Cuts at: 5795 6300 9445 5795 Size: 505 3145 5801 Fragments arranged by size: 5801 3145 505 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4183 4964 7258 7640 Size: 53 83 186 936 2650 781 2294 382 Cuts at: 7640 8275 275 Size: 635 1451 Fragments arranged by size: 2650 2294 1451 936 781 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4704 6162 7884 8282 8556 8703 9114 4704 Size: 1458 1722 398 274 147 411 5041 Fragments arranged by size: 5041 1722 1458 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2239 2269 1178 Size: 30 165 30 836 30 8360 Fragments arranged by size: 8360 836 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2760 5330 1186 Size: 1574 2570 5307 Fragments arranged by size: 5307 2570 1574 BseYI C'CCAG_C Cuts at: 8654 9150 8654 Size: 496 8955 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2421 3693 5118 5349 1163 Size: 573 685 1272 1425 231 5265 Fragments arranged by size: 5265 1425 1272 685 573 231 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6901 7662 8690 1540 Size: 275 186 4900 761 1028 2301 Fragments arranged by size: 4900 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3280 3438 3831 6186 6644 6732 7512 7597 8668 Size: 158 393 2355 458 88 780 85 1071 Cuts at: 8668 3280 Size: 4063 Fragments arranged by size: 4063 2355 1071 780 458 393 158 88 85 BsmI GAATG_Cn' Cuts at: 6962 7055 6962 Size: 93 9358 BsmBI CGTCTCn'nnnn_ Cuts at: 5295 5295 Size: 9451 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2605 2767 3280 3438 3466 3831 3955 Size: 399 815 162 513 158 28 365 124 Cuts at: 3955 5227 6031 6186 6644 6732 6770 7512 7597 Size: 1272 804 155 458 88 38 742 85 Cuts at: 7597 8668 1391 Size: 1071 2174 Fragments arranged by size: 2174 1272 1071 815 804 742 513 458 399 365 162 158 155 124 88 85 38 28 BspEI T'CCGG_A Cuts at: 4704 9114 4704 Size: 4410 5041 BspHI T'CATG_A Cuts at: 2134 5383 6705 7340 9070 2134 Size: 3249 1322 635 1730 2515 Fragments arranged by size: 3249 2515 1730 1322 635 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2064 2396 3469 5293 6603 6806 844 Size: 1220 332 1073 1824 1310 203 3489 Fragments arranged by size: 3489 1824 1310 1220 1073 332 203 BsrBI CCG'CTC Cuts at: 1394 1823 7338 1394 Size: 429 5515 3507 Fragments arranged by size: 5515 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4864 7946 8120 65 Size: 1183 3616 3082 174 1396 Fragments arranged by size: 3616 3082 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6162 8095 8282 1700 Size: 110 4352 1933 187 2869 Fragments arranged by size: 4352 2869 1933 187 110 BsrGI T'GTAC_A Cuts at: 96 6527 96 Size: 6431 3020 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9451 BssSI C'ACGA_G Cuts at: 3107 7511 8523 3107 Size: 4404 1012 4035 Fragments arranged by size: 4404 4035 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3138 794 Size: 2344 7107 BstBI TT'CG_AA Cuts at: 5203 8255 5203 Size: 3052 6399 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9451 BstXI CCAn_nnnn'nTGG Cuts at: 2752 6592 2752 Size: 3840 5611 BstYI r'GATC_y Cuts at: 1972 2044 2062 2269 4364 5637 6480 6781 7148 Size: 72 18 207 2095 1273 843 301 367 Cuts at: 7148 7533 7550 9002 9088 9100 9446 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2095 1977 1452 1273 843 385 367 346 301 207 86 72 18 17 12 BstZ17I GTA'TAC Cuts at: 4657 4657 Size: 9451 Bsu36I CC'TnA_GG Cuts at: 2667 3864 5529 2667 Size: 1197 1665 6589 Fragments arranged by size: 6589 1665 1197 BtgI C'CryG_G Cuts at: 513 1065 2004 5086 513 Size: 552 939 3082 4878 Fragments arranged by size: 4878 3082 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8712 Fragments arranged by size: 8712 683 56 BtsI GCAGTG_nn' Cuts at: 3257 5164 5179 6051 6967 7761 7781 3257 Size: 1907 15 872 916 794 20 4927 Fragments arranged by size: 4927 1907 916 872 794 20 15 ClaI AT'CG_AT Cuts at: 1554 6312 1554 Size: 4758 4693 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3522 3557 3795 3830 5766 5801 529 Size: 35 2958 35 238 35 1936 35 4179 Fragments arranged by size: 4179 2958 1936 238 35 35 35 35 DraI TTT'AAA Cuts at: 2451 4630 6755 2451 Size: 2179 2125 5147 Fragments arranged by size: 5147 2179 2125 DraIII CAC_nnn'GTG Cuts at: 3796 3796 Size: 9451 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2036 8458 809 Size: 507 720 6422 1802 Fragments arranged by size: 6422 1802 720 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6898 7787 9416 Size: 54 1425 50 275 18 5068 889 1629 Cuts at: 9416 8 Size: 43 Fragments arranged by size: 5068 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6898 1537 Size: 275 5086 4090 Fragments arranged by size: 5086 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2259 2807 3195 4420 4743 5161 5208 5273 Size: 906 548 388 1225 323 418 47 65 Cuts at: 5273 6476 6720 7381 1353 Size: 1203 244 661 3423 Fragments arranged by size: 3423 1225 1203 906 661 548 418 388 323 244 65 47 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5744 8038 8412 8558 387 Size: 1064 138 4155 2294 374 146 1280 Fragments arranged by size: 4155 2294 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 6184 6730 6184 Size: 546 8905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2044 2474 2592 2778 3206 3674 3873 Size: 393 412 430 118 186 428 468 199 Cuts at: 3873 4320 4767 5574 5921 5938 6111 6218 6268 Size: 447 447 807 347 17 173 107 50 Cuts at: 6268 6459 6626 7514 8111 8898 9166 1239 Size: 191 167 888 597 787 268 1524 Fragments arranged by size: 1524 888 807 787 597 468 447 447 430 428 412 393 347 268 199 191 186 173 167 118 107 50 17 EcoNI CCTnn'n_nnAGG Cuts at: 2199 5457 5842 2199 Size: 3258 385 5808 Fragments arranged by size: 5808 3258 385 EcoO109I rG'GnC_Cy Cuts at: 2012 2689 3092 3607 2012 Size: 677 403 515 7856 Fragments arranged by size: 7856 677 515 403 EcoRI G'AATT_C Cuts at: 6760 7138 6760 Size: 378 9073 EcoRV GAT'ATC Cuts at: 4204 6302 4204 Size: 2098 7353 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6298 6679 6298 Size: 381 9070 FspI TGC'GCA Cuts at: 6680 7957 6680 Size: 1277 8174 HaeII r_GCGC'y Cuts at: 1678 4186 4967 8598 1678 Size: 2508 781 3631 2531 Fragments arranged by size: 3631 2531 2508 781 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2161 2193 2425 2457 2711 Size: 32 135 32 589 32 232 32 254 Cuts at: 2711 2743 3917 3949 3991 4023 4043 4075 4247 Size: 32 1174 32 42 32 20 32 172 Cuts at: 4247 4279 4799 4831 5251 5283 6089 6121 6291 Size: 32 520 32 420 32 806 32 170 Cuts at: 6291 6305 6317 6323 6337 6349 6491 6523 8092 Size: 14 12 6 14 12 142 32 1569 Cuts at: 8092 8124 8166 8198 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1569 1174 806 589 520 420 254 232 172 170 142 135 42 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 20 14 14 12 12 6 HincII GTy'rAC Cuts at: 669 1657 1938 4606 4719 5566 5937 6244 6795 Size: 988 281 2668 113 847 371 307 551 Cuts at: 6795 7041 669 Size: 246 3079 Fragments arranged by size: 3079 2668 988 847 551 371 307 281 246 113 HindIII A'AGCT_T Cuts at: 748 4145 748 Size: 3397 6054 HpaI GTT'AAC Cuts at: 4719 5566 5937 7041 4719 Size: 847 371 1104 7129 Fragments arranged by size: 7129 1104 847 371 KasI G'GCGC_C Cuts at: 4182 4963 4182 Size: 781 8670 KpnI G_GTAC'C Cuts at: 3554 3584 6776 3554 Size: 30 3192 6229 Fragments arranged by size: 6229 3192 30 MfeI C'AATT_G Cuts at: 3900 5991 7050 3900 Size: 2091 1059 6301 Fragments arranged by size: 6301 2091 1059 MluI A'CGCG_T Cuts at: 9327 9327 Size: 9451 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2090 5989 6239 8565 8749 1305 Size: 785 3899 250 2326 184 2007 Fragments arranged by size: 3899 2326 2007 785 250 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7629 Fragments arranged by size: 7629 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2750 4851 4914 5388 5613 5955 6018 Size: 648 1584 2101 63 474 225 342 63 Cuts at: 6018 6566 7409 7768 7927 518 Size: 548 843 359 159 2042 Fragments arranged by size: 2101 2042 1584 843 648 548 474 359 342 225 159 63 63 MspA1I CmG'CkG Cuts at: 1606 2159 2368 2844 4432 6149 6867 7544 8692 Size: 553 209 476 1588 1717 718 677 1148 Cuts at: 8692 8937 1606 Size: 245 2120 Fragments arranged by size: 2120 1717 1588 1148 718 677 553 476 245 209 NarI GG'CG_CC Cuts at: 4183 4964 4183 Size: 781 8670 NcoI C'CATG_G Cuts at: 513 1065 2004 5086 513 Size: 552 939 3082 4878 Fragments arranged by size: 4878 3082 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9451 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9451 NotI GC'GGCC_GC Cuts at: 6898 6898 Size: 9451 NsiI A_TGCA'T Cuts at: 6025 6071 6025 Size: 46 9405 NspI r_CATG'y Cuts at: 1132 1229 2926 2969 5144 5592 5614 6106 6809 Size: 97 1697 43 2175 448 22 492 703 Cuts at: 6809 8354 1132 Size: 1545 2229 Fragments arranged by size: 2229 2175 1697 1545 703 492 448 97 43 22 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9451 PciI A'CATG_T Cuts at: 2965 5610 6102 8350 2965 Size: 2645 492 2248 4066 Fragments arranged by size: 4066 2645 2248 492 PflMI CCAn_nnn'nTGG Cuts at: 1108 2847 5327 6546 1108 Size: 1739 2480 1219 4013 Fragments arranged by size: 4013 2480 1739 1219 PfoI T'CCnGG_A Cuts at: 5269 5269 Size: 9451 PmeI GTTT'AAAC Cuts at: 6755 6755 Size: 9451 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7520 7552 9033 9065 7520 Size: 32 1481 32 7906 Fragments arranged by size: 7906 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2012 2689 3092 2012 Size: 677 403 8371 Fragments arranged by size: 8371 677 403 PshAI GACnn'nnGTC Cuts at: 1613 5287 1613 Size: 3674 5777 PsiI TTA'TAA Cuts at: 5738 7021 5738 Size: 1283 8168 PstI C_TGCA'G Cuts at: 830 3264 3480 6803 830 Size: 2434 216 3323 3478 Fragments arranged by size: 3478 3323 2434 216 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9451 PvuII CAG'CTG Cuts at: 2159 6149 2159 Size: 3990 5461 SacI G_AGCT'C Cuts at: 6186 6732 6186 Size: 546 8905 SalI G'TCGA_C Cuts at: 1936 6793 1936 Size: 4857 4594 SapI GCTCTTCn'nnn_ Cuts at: 3195 6720 3195 Size: 3525 5926 SbfI CC_TGCA'GG Cuts at: 6803 6803 Size: 9451 ScaI AGT'ACT Cuts at: 2303 7699 9411 2303 Size: 5396 1712 2343 Fragments arranged by size: 5396 2343 1712 SexAI A'CCwGG_T Cuts at: 3546 3633 4875 5837 3546 Size: 87 1242 962 7160 Fragments arranged by size: 7160 1242 962 87 SfcI C'TryA_G Cuts at: 826 1045 3230 3260 3350 3446 3476 5050 5309 Size: 219 2185 30 90 96 30 1574 259 Cuts at: 5309 6513 6799 7934 8615 8806 826 Size: 1204 286 1135 681 191 1471 Fragments arranged by size: 2185 1574 1471 1204 1135 681 286 259 219 191 96 90 30 30 SfoI GGC'GCC Cuts at: 4184 4965 4184 Size: 781 8670 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9451 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9451 SmaI CCC'GGG Cuts at: 1872 6647 6778 1872 Size: 4775 131 4545 Fragments arranged by size: 4775 4545 131 SmlI C'TyrA_G Cuts at: 820 1017 1943 6203 7555 8456 8718 820 Size: 197 926 4260 1352 901 262 1553 Fragments arranged by size: 4260 1553 1352 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 7242 493 Size: 6749 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9451 SphI G_CATG'C Cuts at: 1132 6809 1132 Size: 5677 3774 SspI AAT'ATT Cuts at: 5 52 2076 7375 5 Size: 47 2024 5299 2081 Fragments arranged by size: 5299 2081 2024 47 StuI AGG'CCT Cuts at: 1517 5339 1517 Size: 3822 5629 StyI C'CwwG_G Cuts at: 513 1065 2004 2101 2790 3179 3467 4976 5086 Size: 552 939 97 689 389 288 1509 110 Cuts at: 5086 5227 6890 513 Size: 141 1663 3074 Fragments arranged by size: 3074 1663 1509 939 689 552 389 288 141 110 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2232 2401 2949 7478 7495 7648 7833 Size: 1255 652 169 548 4529 17 153 185 Cuts at: 7833 8278 325 Size: 445 1498 Fragments arranged by size: 4529 1498 1255 652 548 445 185 169 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2301 3069 6527 Size: 274 80 33 51 1443 324 768 3458 Cuts at: 6527 7697 9409 96 Size: 1170 1712 138 Fragments arranged by size: 3458 1712 1443 1170 768 324 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2196 2228 2239 2271 3138 3170 9411 9443 2196 Size: 32 11 32 867 32 6241 32 2204 Fragments arranged by size: 6241 2204 867 32 32 32 32 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9451 XbaI T'CTAG_A Cuts at: 6787 6787 Size: 9451 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4619 4619 Size: 9451 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9451 XmaI C'CCGG_G Cuts at: 1870 6645 6776 1870 Size: 4775 131 4545 Fragments arranged by size: 4775 4545 131 XmnI GAAnn'nnTTC Cuts at: 2487 2636 4281 7580 2487 Size: 149 1645 3299 4358 Fragments arranged by size: 4358 3299 1645 149 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7259 276 Size: 53 83 186 936 5725 2468 Fragments arranged by size: 5725 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BplI BsiWI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI