(Linear) (Six-base) MAPSORT of: KIEE3502.seq Check: 153 from: 1 to: 4746 KIEE3502.orf With 182 enzymes: SgfI * July 3, 2012 17:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 59 4746 Size: 59 4687 AccI GT'mk_AC Cuts at: 0 2651 4746 Size: 2651 2095 Acc65I G'GTAC_C Cuts at: 0 1545 1575 4746 Size: 1545 30 3171 Fragments arranged by size: 3171 1545 30 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 469 587 773 1201 1868 2315 2762 3569 Size: 469 118 186 428 667 447 447 807 Cuts at: 3569 3916 3933 4106 4213 4263 4454 4621 4746 Size: 347 17 173 107 50 191 167 125 Fragments arranged by size: 807 667 469 447 447 428 347 191 186 173 167 125 118 107 50 17 AflIII A'CryG_T Cuts at: 0 960 3605 4097 4746 Size: 960 2645 492 649 Fragments arranged by size: 2645 960 649 492 AgeI A'CCGG_T Cuts at: 0 4157 4746 Size: 4157 589 AhdI GACnn_n'nnGTC Cuts at: 0 3260 4098 4746 Size: 3260 838 648 Fragments arranged by size: 3260 838 648 AleI CACnn'nnGTG Cuts at: 0 3950 4746 Size: 3950 796 AlwNI CAG_nnn'CTG Cuts at: 0 1263 1717 3358 3373 4084 4318 4746 Size: 1263 454 1641 15 711 234 428 Fragments arranged by size: 1641 1263 711 454 428 234 15 ApaLI G'TGCA_C Cuts at: 0 1271 4635 4746 Size: 1271 3364 111 Fragments arranged by size: 3364 1271 111 ApoI r'AATT_y Cuts at: 0 888 1555 4746 Size: 888 667 3191 Fragments arranged by size: 3191 888 667 AvaI C'yCGr_G Cuts at: 0 1328 4640 4746 Size: 1328 3312 106 Fragments arranged by size: 3312 1328 106 AvrII C'CTAG_G Cuts at: 0 1462 4746 Size: 1462 3284 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1805 1838 4746 Size: 1805 33 2908 Fragments arranged by size: 2908 1805 33 BamHI G'GATC_C Cuts at: 0 57 4746 Size: 57 4689 BanI G'GyrC_C Cuts at: 0 365 657 1302 1398 1458 1545 1575 1632 Size: 365 292 645 96 60 87 30 57 Cuts at: 1632 1662 1947 2177 2721 2958 4023 4746 Size: 30 285 230 544 237 1065 723 Fragments arranged by size: 1065 723 645 544 365 292 285 237 230 96 87 60 57 30 30 BanII G_rGCy'C Cuts at: 0 762 3222 4181 4727 4746 Size: 762 2460 959 546 19 Fragments arranged by size: 2460 959 762 546 19 BbeI G_GCGC'C Cuts at: 0 2181 2962 4746 Size: 2181 781 1784 Fragments arranged by size: 2181 1784 781 BbsI GAAGACnn'nnnn_ Cuts at: 0 142 3747 3904 4746 Size: 142 3605 157 842 Fragments arranged by size: 3605 842 157 142 BbvCI CC'TCA_GC Cuts at: 0 2423 4746 Size: 2423 2323 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 386 420 4746 Size: 386 34 4326 Fragments arranged by size: 4326 386 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1500 1806 4746 Size: 1500 306 2940 Fragments arranged by size: 2940 1500 306 BclI T'GATC_A Cuts at: 0 2583 2838 2877 3375 4068 4218 4746 Size: 2583 255 39 498 693 150 528 Fragments arranged by size: 2583 693 528 498 255 150 39 BfrBI ATG'CAT Cuts at: 0 4018 4064 4746 Size: 4018 46 682 Fragments arranged by size: 4018 682 46 BglI GCCn_nnn'nGGC Cuts at: 0 1179 4746 Size: 1179 3567 BglII A'GATC_T Cuts at: 0 2359 3632 4746 Size: 2359 1273 1114 Fragments arranged by size: 2359 1273 1114 BlpI GC'TnA_GC Cuts at: 0 492 4284 4746 Size: 492 3792 462 Fragments arranged by size: 3792 492 462 Bme1580I G_kGCm'C Cuts at: 0 600 1275 1461 1950 4026 4639 4746 Size: 600 675 186 489 2076 613 107 Fragments arranged by size: 2076 675 613 600 489 186 107 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 39 1669 4746 Size: 39 1630 3077 Fragments arranged by size: 3077 1630 39 Bpu10I CC'TnA_GC Cuts at: 0 1277 1298 1352 2423 3536 4746 Size: 1277 21 54 1071 1113 1210 Fragments arranged by size: 1277 1210 1113 1071 54 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 4183 4746 Size: 4183 563 BsaI GGTCTCn'nnnn_ Cuts at: 0 2124 2435 2558 3270 4161 4746 Size: 2124 311 123 712 891 585 Fragments arranged by size: 2124 891 712 585 311 123 BsaBI GATnn'nnATC Cuts at: 0 3790 4295 4746 Size: 3790 505 451 Fragments arranged by size: 3790 505 451 BsaHI Gr'CG_yC Cuts at: 0 2178 2959 4746 Size: 2178 781 1787 Fragments arranged by size: 2178 1787 781 BsaWI w'CCGG_w Cuts at: 0 2699 4157 4746 Size: 2699 1458 589 Fragments arranged by size: 2699 1458 589 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 234 264 4746 Size: 234 30 4482 Fragments arranged by size: 4482 234 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 755 3325 4746 Size: 755 2570 1421 Fragments arranged by size: 2570 1421 755 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 416 1688 3113 3344 4746 Size: 416 1272 1425 231 1402 Fragments arranged by size: 1425 1402 1272 416 231 BsiHKAI G_wGCw'C Cuts at: 0 1275 1433 1826 4181 4639 4727 4746 Size: 1275 158 393 2355 458 88 19 Fragments arranged by size: 2355 1275 458 393 158 88 19 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3290 4746 Size: 3290 1456 Bsp1286I G_dGCh'C Cuts at: 0 600 762 1275 1433 1461 1826 1950 3222 Size: 600 162 513 158 28 365 124 1272 Cuts at: 3222 4026 4181 4639 4727 4746 Size: 804 155 458 88 19 Fragments arranged by size: 1272 804 600 513 458 365 162 158 155 124 88 28 19 BspEI T'CCGG_A Cuts at: 0 2699 4746 Size: 2699 2047 BspHI T'CATG_A Cuts at: 0 129 3378 4700 4746 Size: 129 3249 1322 46 Fragments arranged by size: 3249 1322 129 46 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 59 391 1464 3288 4598 4746 Size: 59 332 1073 1824 1310 148 Fragments arranged by size: 1824 1310 1073 332 148 59 BsrDI GCAATG_nn' Cuts at: 0 2859 4746 Size: 2859 1887 BsrFI r'CCGG_y Cuts at: 0 4157 4746 Size: 4157 589 BsrGI T'GTAC_A Cuts at: 0 4522 4746 Size: 4522 224 BssSI C'ACGA_G Cuts at: 0 1102 4746 Size: 1102 3644 BstAPI GCAn_nnn'nTGC Cuts at: 0 1133 4746 Size: 1133 3613 BstBI TT'CG_AA Cuts at: 0 3198 4746 Size: 3198 1548 BstXI CCAn_nnnn'nTGG Cuts at: 0 747 4587 4746 Size: 747 3840 159 Fragments arranged by size: 3840 747 159 BstYI r'GATC_y Cuts at: 0 39 57 264 2359 3632 4475 4746 Size: 39 18 207 2095 1273 843 271 Fragments arranged by size: 2095 1273 843 271 207 39 18 BstZ17I GTA'TAC Cuts at: 0 2652 4746 Size: 2652 2094 Bsu36I CC'TnA_GG Cuts at: 0 662 1859 3524 4746 Size: 662 1197 1665 1222 Fragments arranged by size: 1665 1222 1197 662 BtgI C'CryG_G Cuts at: 0 3081 4746 Size: 3081 1665 BtsI GCAGTG_nn' Cuts at: 0 1252 3159 3174 4046 4746 Size: 1252 1907 15 872 700 Fragments arranged by size: 1907 1252 872 700 15 ClaI AT'CG_AT Cuts at: 0 4307 4746 Size: 4307 439 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1517 1552 1790 1825 3761 3796 4746 Size: 1517 35 238 35 1936 35 950 Fragments arranged by size: 1936 1517 950 238 35 35 35 DraI TTT'AAA Cuts at: 0 446 2625 4746 Size: 446 2179 2121 Fragments arranged by size: 2179 2121 446 DraIII CAC_nnn'GTG Cuts at: 0 1791 4746 Size: 1791 2955 DrdI GACnn_nn'nnGTC Cuts at: 0 31 4746 Size: 31 4715 EarI CTCTTCn'nnn_ Cuts at: 0 254 802 1190 2415 2738 3156 3203 3268 Size: 254 548 388 1225 323 418 47 65 Cuts at: 3268 4471 4715 4746 Size: 1203 244 31 Fragments arranged by size: 1225 1203 548 418 388 323 254 244 65 47 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3739 4746 Size: 3739 1007 EcoICRI GAG'CTC Cuts at: 0 4179 4725 4746 Size: 4179 546 21 Fragments arranged by size: 4179 546 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 39 469 587 773 1201 1669 1868 2315 Size: 39 430 118 186 428 468 199 447 Cuts at: 2315 2762 3569 3916 3933 4106 4213 4263 4454 Size: 447 807 347 17 173 107 50 191 Cuts at: 4454 4621 4746 Size: 167 125 Fragments arranged by size: 807 468 447 447 430 428 347 199 191 186 173 167 125 118 107 50 39 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 194 3452 3837 4746 Size: 194 3258 385 909 Fragments arranged by size: 3258 909 385 194 EcoO109I rG'GnC_Cy Cuts at: 0 7 684 1087 1602 4746 Size: 7 677 403 515 3144 Fragments arranged by size: 3144 677 515 403 7 EcoRV GAT'ATC Cuts at: 0 2199 4297 4746 Size: 2199 2098 449 Fragments arranged by size: 2199 2098 449 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 4293 4674 4746 Size: 4293 381 72 Fragments arranged by size: 4293 381 72 FspI TGC'GCA Cuts at: 0 4675 4746 Size: 4675 71 HaeII r_GCGC'y Cuts at: 0 2181 2962 4746 Size: 2181 781 1784 Fragments arranged by size: 2181 1784 781 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 156 188 420 452 706 738 1912 1944 Size: 156 32 232 32 254 32 1174 32 Cuts at: 1944 1986 2018 2038 2070 2242 2274 2794 2826 Size: 42 32 20 32 172 32 520 32 Cuts at: 2826 3246 3278 4084 4116 4286 4300 4312 4318 Size: 420 32 806 32 170 14 12 6 Cuts at: 4318 4332 4344 4486 4518 4746 Size: 14 12 142 32 228 Fragments arranged by size: 1174 806 520 420 254 232 228 172 170 156 142 42 32 32 32 32 32 32 32 32 32 32 32 20 14 14 12 12 6 HincII GTy'rAC Cuts at: 0 2601 2714 3561 3932 4239 4746 Size: 2601 113 847 371 307 507 Fragments arranged by size: 2601 847 507 371 307 113 HindIII A'AGCT_T Cuts at: 0 2140 4746 Size: 2140 2606 HpaI GTT'AAC Cuts at: 0 2714 3561 3932 4746 Size: 2714 847 371 814 Fragments arranged by size: 2714 847 814 371 KasI G'GCGC_C Cuts at: 0 2177 2958 4746 Size: 2177 781 1788 Fragments arranged by size: 2177 1788 781 KpnI G_GTAC'C Cuts at: 0 1549 1579 4746 Size: 1549 30 3167 Fragments arranged by size: 3167 1549 30 MfeI C'AATT_G Cuts at: 0 1895 3986 4746 Size: 1895 2091 760 Fragments arranged by size: 2091 1895 760 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 85 3984 4234 4746 Size: 85 3899 250 512 Fragments arranged by size: 3899 512 250 85 MslI CAynn'nnrTG Cuts at: 0 745 2846 2909 3383 3608 3950 4013 4561 Size: 745 2101 63 474 225 342 63 548 Cuts at: 4561 4746 Size: 185 Fragments arranged by size: 2101 745 548 474 342 225 185 63 63 MspA1I CmG'CkG Cuts at: 0 154 363 839 2427 4144 4746 Size: 154 209 476 1588 1717 602 Fragments arranged by size: 1717 1588 602 476 209 154 NarI GG'CG_CC Cuts at: 0 2178 2959 4746 Size: 2178 781 1787 Fragments arranged by size: 2178 1787 781 NcoI C'CATG_G Cuts at: 0 3081 4746 Size: 3081 1665 NsiI A_TGCA'T Cuts at: 0 4020 4066 4746 Size: 4020 46 680 Fragments arranged by size: 4020 680 46 NspI r_CATG'y Cuts at: 0 921 964 3139 3587 3609 4101 4746 Size: 921 43 2175 448 22 492 645 Fragments arranged by size: 2175 921 645 492 448 43 22 PciI A'CATG_T Cuts at: 0 960 3605 4097 4746 Size: 960 2645 492 649 Fragments arranged by size: 2645 960 649 492 PflMI CCAn_nnn'nTGG Cuts at: 0 842 3322 4541 4746 Size: 842 2480 1219 205 Fragments arranged by size: 2480 1219 842 205 PfoI T'CCnGG_A Cuts at: 0 3264 4746 Size: 3264 1482 PpuMI rG'GwC_Cy Cuts at: 0 7 684 1087 4746 Size: 7 677 403 3659 Fragments arranged by size: 3659 677 403 7 PshAI GACnn'nnGTC Cuts at: 0 3282 4746 Size: 3282 1464 PsiI TTA'TAA Cuts at: 0 3733 4746 Size: 3733 1013 PstI C_TGCA'G Cuts at: 0 1259 1475 4746 Size: 1259 216 3271 Fragments arranged by size: 3271 1259 216 PvuII CAG'CTG Cuts at: 0 154 4144 4746 Size: 154 3990 602 Fragments arranged by size: 3990 602 154 SacI G_AGCT'C Cuts at: 0 4181 4727 4746 Size: 4181 546 19 Fragments arranged by size: 4181 546 19 SapI GCTCTTCn'nnn_ Cuts at: 0 1190 4715 4746 Size: 1190 3525 31 Fragments arranged by size: 3525 1190 31 ScaI AGT'ACT Cuts at: 0 298 4746 Size: 298 4448 SexAI A'CCwGG_T Cuts at: 0 1541 1628 2870 3832 4746 Size: 1541 87 1242 962 914 Fragments arranged by size: 1541 1242 962 914 87 SfcI C'TryA_G Cuts at: 0 1225 1255 1345 1441 1471 3045 3304 4508 Size: 1225 30 90 96 30 1574 259 1204 Cuts at: 4508 4746 Size: 238 Fragments arranged by size: 1574 1225 1204 259 238 96 90 30 30 SfoI GGC'GCC Cuts at: 0 2179 2960 4746 Size: 2179 781 1786 Fragments arranged by size: 2179 1786 781 SmaI CCC'GGG Cuts at: 0 4642 4746 Size: 4642 104 SmlI C'TyrA_G Cuts at: 0 4198 4746 Size: 4198 548 SspI AAT'ATT Cuts at: 0 71 4746 Size: 71 4675 StuI AGG'CCT Cuts at: 0 3334 4746 Size: 3334 1412 StyI C'CwwG_G Cuts at: 0 96 785 1174 1462 2971 3081 3222 4746 Size: 96 689 389 288 1509 110 141 1524 Fragments arranged by size: 1524 1509 689 389 288 141 110 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 227 396 944 4746 Size: 227 169 548 3802 Fragments arranged by size: 3802 548 227 169 TatI w'GTAC_w Cuts at: 0 296 1064 4522 4746 Size: 296 768 3458 224 Fragments arranged by size: 3458 768 296 224 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 191 223 234 266 1133 1165 4746 Size: 191 32 11 32 867 32 3581 Fragments arranged by size: 3581 867 191 32 32 32 11 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2614 4746 Size: 2614 2132 XmaI C'CCGG_G Cuts at: 0 4640 4746 Size: 4640 106 XmnI GAAnn'nnTTC Cuts at: 0 482 631 2276 4746 Size: 482 149 1645 2470 Fragments arranged by size: 2470 1645 482 149 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AgeI AhdI AleI AlwNI ApaLI ApoI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MmeI MslI MspA1I NarI NcoI NsiI NspI PciI PflMI PfoI PpuMI PshAI PsiI PstI PvuII SacI SapI ScaI SexAI SfcI SfoI SmaI SmlI SspI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AlfI AloI ApaI AscI AseI AsiSI BmgBI BmrI BmtI BplI BsaAI BseYI BsiEI BsiWI BsmI BsrBI BssHII BstEII BtgZI EaeI EagI EcoRI FseI FspAI MluI MscI NaeI NdeI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI ZraI