(Circular) (Six-base) MAPSORT of: pFN21AE3451.seq Check: 6945 from: 1 to: 8668 pFN21AE3451 8668 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3893 6478 278 Size: 53 83 186 936 2357 2585 2468 Fragments arranged by size: 2585 2468 2357 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6011 1937 Size: 4074 4594 Acc65I G'GTAC_C Cuts at: 5989 5989 Size: 8668 AclI AA'CG_TT Cuts at: 1563 6795 7168 1563 Size: 5232 373 3063 Fragments arranged by size: 5232 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2807 3216 6731 8115 8383 1632 Size: 1175 409 3515 1384 268 1917 Fragments arranged by size: 3515 1917 1384 1175 409 268 AfeI AGC'GCT Cuts at: 4870 4870 Size: 8668 AflII C'TTAA_G Cuts at: 820 1017 2027 820 Size: 197 1010 7461 Fragments arranged by size: 7461 1010 197 AflIII A'CryG_T Cuts at: 1203 3543 7567 8544 1203 Size: 2340 4024 977 1327 Fragments arranged by size: 4024 2340 1327 977 AgeI A'CCGG_T Cuts at: 7499 7499 Size: 8668 AhdI GACnn_n'nnGTC Cuts at: 2671 7397 2671 Size: 4726 3942 AleI CACnn'nnGTG Cuts at: 1166 3096 1166 Size: 1930 6738 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2414 5376 5516 1184 Size: 1230 2962 140 4336 Fragments arranged by size: 4336 2962 1230 140 AlwNI CAG_nnn'CTG Cuts at: 3044 3493 3554 3680 3811 3850 5269 5764 6411 Size: 449 61 126 131 39 1419 495 647 Cuts at: 6411 7983 3044 Size: 1572 3729 Fragments arranged by size: 3729 1572 1419 647 495 449 131 126 61 39 ApaI G_GGCC'C Cuts at: 3155 4930 5008 5657 5848 3155 Size: 1775 78 649 191 5975 Fragments arranged by size: 5975 1775 649 191 78 ApaLI G'TGCA_C Cuts at: 4796 6725 7881 4796 Size: 1929 1156 5583 Fragments arranged by size: 5583 1929 1156 ApoI r'AATT_y Cuts at: 1493 5977 6203 6355 8464 1493 Size: 4484 226 152 2109 1697 Fragments arranged by size: 4484 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7222 160 Size: 872 6190 1606 Fragments arranged by size: 6190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8668 AvaI C'yCGr_G Cuts at: 1870 1943 3128 3179 3716 3737 3789 3937 4696 Size: 73 1185 51 537 21 52 148 759 Cuts at: 4696 4929 5010 5807 5823 5841 5870 5993 1870 Size: 233 81 797 16 18 29 123 4545 Fragments arranged by size: 4545 1185 797 759 537 233 148 123 81 73 52 51 29 21 18 16 BamHI G'GATC_C Cuts at: 5998 6365 5998 Size: 367 8301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2375 2945 3592 3698 4003 Size: 325 217 627 588 570 647 106 305 Cuts at: 4003 4412 4583 4658 5858 5989 7444 618 Size: 409 171 75 1200 131 1455 1842 Fragments arranged by size: 1842 1455 1200 647 627 588 570 409 325 305 217 171 131 106 75 BanII G_rGCy'C Cuts at: 1391 3155 3660 3687 3717 3777 4502 4689 4930 Size: 1764 505 27 30 60 725 187 241 Cuts at: 4930 4991 5008 5204 5211 5254 5390 5657 5668 Size: 61 17 196 7 43 136 267 11 Cuts at: 5668 5783 5848 5879 5987 1391 Size: 115 65 31 108 4072 Fragments arranged by size: 4072 1764 725 505 267 241 196 187 136 115 108 65 61 60 43 31 30 27 17 11 7 BbeI G_GCGC'C Cuts at: 4007 4007 Size: 8668 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4824 928 Size: 971 2925 4772 Fragments arranged by size: 4772 2925 971 BbvCI CC'TCA_GC Cuts at: 3603 4605 3603 Size: 1002 7666 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6859 6893 1903 Size: 34 50 34 4838 34 3678 Fragments arranged by size: 4838 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5180 6560 7770 5180 Size: 1380 1210 6078 Fragments arranged by size: 6078 1380 1210 BclI T'GATC_A Cuts at: 1548 2754 2868 1548 Size: 1206 114 7348 Fragments arranged by size: 7348 1206 114 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3133 4469 4562 Size: 107 122 71 631 605 1461 1336 93 Cuts at: 4562 4699 5327 5484 7279 136 Size: 137 628 157 1795 1525 Fragments arranged by size: 1795 1525 1461 1336 631 628 605 157 137 122 107 93 71 BglII A'GATC_T Cuts at: 2145 2415 8663 2145 Size: 270 6248 2150 Fragments arranged by size: 6248 2150 270 BlpI GC'TnA_GC Cuts at: 2387 3681 3863 5910 6085 2387 Size: 1294 182 2047 175 4970 Fragments arranged by size: 4970 2047 1294 182 175 Bme1580I G_kGCm'C Cuts at: 1790 3155 3316 3701 4417 4586 4800 4930 5008 Size: 1365 161 385 716 169 214 130 78 Cuts at: 5008 5657 5848 6729 7885 1790 Size: 649 191 881 1156 2573 Fragments arranged by size: 2573 1365 1156 881 716 649 385 214 191 169 161 130 78 BmgBI CAC'GTC Cuts at: 1318 3544 1318 Size: 2226 6442 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2941 3150 3299 Size: 416 53 83 399 24 1524 209 149 Cuts at: 3299 3332 6399 7357 442 Size: 33 3067 958 1753 Fragments arranged by size: 3067 1753 1524 958 416 399 209 149 83 53 33 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8668 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4516 4915 5392 5800 5918 4516 Size: 399 477 408 118 7266 Fragments arranged by size: 7266 477 408 399 118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 2740 3657 4351 4505 4910 4961 5114 Size: 1076 425 917 694 154 405 51 153 Cuts at: 5114 5326 5564 5888 7328 1239 Size: 212 238 324 1440 2579 Fragments arranged by size: 2579 1440 1076 917 694 425 405 324 238 212 154 153 51 Bpu10I CC'TnA_GC Cuts at: 3572 3603 4605 3572 Size: 31 1002 7635 Fragments arranged by size: 7635 1002 31 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2174 2210 2231 3644 4661 6793 7658 7956 2174 Size: 36 21 1413 1017 2132 865 298 2886 Fragments arranged by size: 2886 2132 1413 1017 865 298 36 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4068 5363 7331 882 Size: 617 2569 1295 1968 2219 Fragments arranged by size: 2569 2219 1968 1295 617 BsaAI yAC'GTr Cuts at: 493 1204 2089 6459 493 Size: 711 885 4370 2702 Fragments arranged by size: 4370 2702 885 711 BsaBI GATnn'nnATC Cuts at: 3242 8662 3242 Size: 5420 3248 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3035 3890 4004 5134 Size: 53 83 186 936 1502 855 114 1130 Cuts at: 5134 5591 5829 6475 6857 7492 275 Size: 457 238 646 382 635 1451 Fragments arranged by size: 1502 1451 1130 936 855 646 635 457 382 238 186 114 83 53 BsaWI w'CCGG_w Cuts at: 2775 3222 3234 3357 4119 4398 4835 7101 7499 Size: 447 12 123 762 279 437 2266 398 Cuts at: 7499 7773 7920 8331 2775 Size: 274 147 411 3112 Fragments arranged by size: 3112 2266 762 447 437 411 398 279 274 147 123 12 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8443 Fragments arranged by size: 8443 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3221 3281 3439 3463 3620 3623 3725 3734 Size: 2035 60 158 24 157 3 102 9 Cuts at: 3734 3755 3833 4223 4226 4229 4232 4277 4292 Size: 21 78 390 3 3 3 45 15 Cuts at: 4292 4295 4307 4310 4313 4322 4325 4328 4402 Size: 3 12 3 3 9 3 3 74 Cuts at: 4402 4508 4593 4753 4874 4934 5204 5329 5363 Size: 106 85 160 121 60 270 125 34 Cuts at: 5363 5408 1186 Size: 45 4446 Fragments arranged by size: 4446 2035 390 270 160 158 157 125 121 106 102 85 78 74 60 60 45 45 34 24 21 15 12 9 9 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2205 2404 2985 3148 3296 3686 3763 3843 3858 Size: 199 581 163 148 390 77 80 15 Cuts at: 3858 4348 4620 4714 4871 4882 5514 5548 5686 Size: 490 272 94 157 11 632 34 138 Cuts at: 5686 5847 5957 7871 8367 2205 Size: 161 110 1914 496 2506 Fragments arranged by size: 2506 1914 632 581 496 490 390 272 199 163 161 157 148 138 110 94 80 77 34 15 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2296 2441 3917 1163 Size: 573 560 145 1476 5914 Fragments arranged by size: 5914 1476 573 560 145 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 5258 6118 6879 7907 1540 Size: 275 186 21 3236 860 761 1028 2301 Fragments arranged by size: 3236 2301 1028 860 761 275 186 21 BsiHKAI G_wGCw'C Cuts at: 2247 2393 2775 2972 3093 3660 4394 4800 6729 Size: 146 382 197 121 567 734 406 1929 Cuts at: 6729 6814 7885 2247 Size: 85 1071 3030 Fragments arranged by size: 3030 1929 1071 734 567 406 382 197 146 121 85 BsmI GAATG_Cn' Cuts at: 3115 6179 6272 3115 Size: 3064 93 5511 Fragments arranged by size: 5511 3064 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3644 3881 3644 Size: 237 8431 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2247 2393 2775 2972 3093 3155 3316 Size: 399 457 146 382 197 121 62 161 Cuts at: 3316 3660 3687 3701 3717 3777 4394 4417 4502 Size: 344 27 14 16 60 617 23 85 Cuts at: 4502 4586 4689 4800 4930 4991 5008 5204 5211 Size: 84 103 111 130 61 17 196 7 Cuts at: 5211 5254 5390 5657 5668 5783 5848 5879 5987 Size: 43 136 267 11 115 65 31 108 Cuts at: 5987 6729 6814 7885 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 617 457 399 382 344 267 197 196 161 146 136 130 121 115 111 108 103 85 85 84 65 62 61 60 43 31 27 23 17 16 14 11 7 BspEI T'CCGG_A Cuts at: 3234 4119 8331 3234 Size: 885 4212 3571 Fragments arranged by size: 4212 3571 885 BspHI T'CATG_A Cuts at: 6557 8287 6557 Size: 1730 6938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2172 2209 2268 2361 3060 3834 6023 844 Size: 1328 37 59 93 699 774 2189 3489 Fragments arranged by size: 3489 2189 1328 774 699 93 59 37 BsrBI CCG'CTC Cuts at: 1394 1823 3588 3754 4615 5236 5442 6555 1394 Size: 429 1765 166 861 621 206 1113 3507 Fragments arranged by size: 3507 1765 1113 861 621 429 206 166 BsrDI GCAATG_nn' Cuts at: 65 1248 2396 2826 2907 4800 5740 7163 7337 Size: 1183 1148 430 81 1893 940 1423 174 Cuts at: 7337 65 Size: 1396 Fragments arranged by size: 1893 1423 1396 1183 1148 940 430 174 81 BsrFI r'CCGG_y Cuts at: 1700 1810 2077 5416 5836 7312 7499 1700 Size: 110 267 3339 420 1476 187 2869 Fragments arranged by size: 3339 2869 1476 420 267 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8668 BssHII G'CGCG_C Cuts at: 1925 5729 5914 1925 Size: 3804 185 4679 Fragments arranged by size: 4679 3804 185 BssSI C'ACGA_G Cuts at: 2965 4032 6728 7740 2965 Size: 1067 2696 1012 3893 Fragments arranged by size: 3893 2696 1067 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2779 3835 4144 794 Size: 1985 1056 309 5318 Fragments arranged by size: 5318 1985 1056 309 BstBI TT'CG_AA Cuts at: 7472 7472 Size: 8668 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8668 BstXI CCAn_nnnn'nTGG Cuts at: 2089 3875 4429 4510 5855 2089 Size: 1786 554 81 1345 4902 Fragments arranged by size: 4902 1786 1345 554 81 BstYI r'GATC_y Cuts at: 1972 2145 2415 3439 5531 5998 6365 6750 6767 Size: 173 270 1024 2092 467 367 385 17 Cuts at: 6767 8219 8305 8317 8663 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2092 1977 1452 1024 467 385 367 346 270 173 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3189 4185 4491 5212 5323 3189 Size: 996 306 721 111 6534 Fragments arranged by size: 6534 996 721 306 111 BtgI C'CryG_G Cuts at: 513 1065 2157 2487 2562 3393 3702 3999 4467 Size: 552 1092 330 75 831 309 297 468 Cuts at: 4467 4626 5536 5644 513 Size: 159 910 108 3537 Fragments arranged by size: 3537 1092 910 831 552 468 330 309 297 159 108 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3888 487 Size: 683 56 2662 5267 Fragments arranged by size: 5267 2662 683 56 BtsI GCAGTG_nn' Cuts at: 2015 2709 3731 4378 4551 5042 5607 6184 6978 Size: 694 1022 647 173 491 565 577 794 Cuts at: 6978 6998 2015 Size: 20 3685 Fragments arranged by size: 3685 1022 794 694 647 577 565 491 173 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2029 2064 529 Size: 35 1465 35 7133 Fragments arranged by size: 7133 1465 35 35 DraI TTT'AAA Cuts at: 5972 5972 Size: 8668 DraIII CAC_nnn'GTG Cuts at: 2446 2446 Size: 8668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7675 809 Size: 507 6359 1802 Fragments arranged by size: 6359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2079 2238 Size: 54 1425 50 275 18 189 60 159 Cuts at: 2238 2346 2592 3532 3728 3877 4470 5031 5115 Size: 108 246 940 196 149 593 561 84 Cuts at: 5115 5226 5255 5604 5641 6115 7004 8633 8 Size: 111 29 349 37 474 889 1629 43 Fragments arranged by size: 1629 1425 940 889 593 561 474 349 275 246 196 189 159 149 111 108 84 60 54 50 43 37 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2019 5255 6115 1537 Size: 275 207 3236 860 4090 Fragments arranged by size: 4090 3236 860 275 207 EarI CTCTTCn'nnn_ Cuts at: 1353 2684 2819 3744 4200 4227 4236 4254 4263 Size: 1331 135 925 456 27 9 18 9 Cuts at: 4263 4269 4284 4299 5285 5432 6598 1353 Size: 6 15 15 986 147 1166 3423 Fragments arranged by size: 3423 1331 1166 986 925 456 147 135 27 18 15 15 9 9 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3413 3830 5612 5713 7255 7629 Size: 1064 138 1824 417 1782 101 1542 374 Cuts at: 7629 7775 387 Size: 146 1280 Fragments arranged by size: 1824 1782 1542 1280 1064 417 374 146 138 101 EcoICRI GAG'CTC Cuts at: 3658 3658 Size: 8668 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2740 2807 3216 3657 4351 4505 Size: 393 683 425 67 409 441 694 154 Cuts at: 4505 4910 4961 5114 5326 5564 5888 6731 7328 Size: 405 51 153 212 238 324 843 597 Cuts at: 7328 8115 8383 1239 Size: 787 268 1524 Fragments arranged by size: 1524 843 787 694 683 597 441 425 409 405 393 324 268 238 212 154 153 67 51 EcoNI CCTnn'n_nnAGG Cuts at: 4707 5431 4707 Size: 724 7944 EcoO109I rG'GnC_Cy Cuts at: 2221 3049 3135 3152 3185 3292 3568 3823 3941 Size: 828 86 17 33 107 276 255 118 Cuts at: 3941 4162 4352 4480 4700 4788 4926 5004 5005 Size: 221 190 128 220 88 138 78 1 Cuts at: 5005 5266 5328 5682 5812 5888 2221 Size: 261 62 354 130 76 5001 Fragments arranged by size: 5001 828 354 276 261 255 221 220 190 138 130 128 118 107 88 86 78 76 62 33 17 1 EcoRI G'AATT_C Cuts at: 5977 6355 5977 Size: 378 8290 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2434 2434 Size: 8668 FspI TGC'GCA Cuts at: 7174 7174 Size: 8668 HaeII r_GCGC'y Cuts at: 1678 3812 4007 4872 4914 4937 5359 5693 7815 Size: 2134 195 865 42 23 422 334 2122 Cuts at: 7815 1678 Size: 2531 Fragments arranged by size: 2531 2134 2122 865 422 334 195 42 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2657 2689 3413 3445 5186 Size: 32 135 32 1085 32 724 32 1741 Cuts at: 5186 5218 7309 7341 7383 7415 1373 Size: 32 2091 32 42 32 2626 Fragments arranged by size: 2626 2091 1741 1085 724 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3220 6012 6258 669 Size: 988 281 1282 2792 246 3079 Fragments arranged by size: 3079 2792 1282 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8668 HpaI GTT'AAC Cuts at: 6258 6258 Size: 8668 KasI G'GCGC_C Cuts at: 4003 4003 Size: 8668 KpnI G_GTAC'C Cuts at: 5993 5993 Size: 8668 MfeI C'AATT_G Cuts at: 6267 6267 Size: 8668 MluI A'CGCG_T Cuts at: 8544 8544 Size: 8668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3945 7782 7966 1305 Size: 2640 3837 184 2007 Fragments arranged by size: 3837 2640 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5033 5117 10 Size: 54 1425 343 3201 84 3561 Fragments arranged by size: 3561 3201 1425 343 84 54 MslI CAynn'nnrTG Cuts at: 518 1166 2690 2876 3096 3542 5498 6626 6985 Size: 648 1524 186 220 446 1956 1128 359 Cuts at: 6985 7144 518 Size: 159 2042 Fragments arranged by size: 2042 1956 1524 1128 648 446 359 220 186 159 MspA1I CmG'CkG Cuts at: 1606 2713 3598 3862 4325 4639 5092 5423 5584 Size: 1107 885 264 463 314 453 331 161 Cuts at: 5584 5646 6084 6761 7909 8154 1606 Size: 62 438 677 1148 245 2120 Fragments arranged by size: 2120 1148 1107 885 677 463 453 438 331 314 264 245 161 62 NaeI GCC'GGC Cuts at: 2079 5418 5838 2079 Size: 3339 420 4909 Fragments arranged by size: 4909 3339 420 NarI GG'CG_CC Cuts at: 4004 4004 Size: 8668 NcoI C'CATG_G Cuts at: 513 1065 2157 2487 4467 5536 513 Size: 552 1092 330 1980 1069 3645 Fragments arranged by size: 3645 1980 1092 1069 552 330 NdeI CA'TA_TG Cuts at: 387 387 Size: 8668 NgoMIV G'CCGG_C Cuts at: 2077 5416 5836 2077 Size: 3339 420 4909 Fragments arranged by size: 4909 3339 420 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8668 NotI GC'GGCC_GC Cuts at: 2019 6115 2019 Size: 4096 4572 NspI r_CATG'y Cuts at: 1132 1229 2689 2797 3805 4144 5497 6026 7571 Size: 97 1460 108 1008 339 1353 529 1545 Cuts at: 7571 1132 Size: 2229 Fragments arranged by size: 2229 1545 1460 1353 1008 529 339 108 97 PasI CC'CwG_GG Cuts at: 1348 5597 5657 1348 Size: 4249 60 4359 Fragments arranged by size: 4359 4249 60 PciI A'CATG_T Cuts at: 7567 7567 Size: 8668 PflMI CCAn_nnn'nTGG Cuts at: 1108 2446 3044 1108 Size: 1338 598 6732 Fragments arranged by size: 6732 1338 598 PfoI T'CCnGG_A Cuts at: 2817 3057 2817 Size: 240 8428 PmeI GTTT'AAAC Cuts at: 5972 5972 Size: 8668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2595 2627 6737 6769 8250 8282 2595 Size: 32 4110 32 1481 32 2981 Fragments arranged by size: 4110 2981 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2221 3049 3185 3292 3823 3941 4162 4480 4788 Size: 828 136 107 531 118 221 318 308 Cuts at: 4788 5266 5812 5888 2221 Size: 478 546 76 5001 Fragments arranged by size: 5001 828 546 531 478 318 308 221 136 118 107 76 PshAI GACnn'nnGTC Cuts at: 1613 3215 1613 Size: 1602 7066 PsiI TTA'TAA Cuts at: 6238 6238 Size: 8668 PspOMI G'GGCC_C Cuts at: 3151 4926 5004 5653 5844 3151 Size: 1775 78 649 191 5975 Fragments arranged by size: 5975 1775 649 191 78 PstI C_TGCA'G Cuts at: 830 3831 4324 5589 6020 830 Size: 3001 493 1265 431 3478 Fragments arranged by size: 3478 3001 1265 493 431 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8668 PvuII CAG'CTG Cuts at: 2713 3862 4639 5584 2713 Size: 1149 777 945 5797 Fragments arranged by size: 5797 1149 945 777 SacI G_AGCT'C Cuts at: 3660 3660 Size: 8668 SacII CC_GC'GG Cuts at: 5647 5647 Size: 8668 SalI G'TCGA_C Cuts at: 1936 6010 1936 Size: 4074 4594 SanDI GG'GwC_CC Cuts at: 3292 3941 5812 3292 Size: 649 1871 6148 Fragments arranged by size: 6148 1871 649 SapI GCTCTTCn'nnn_ Cuts at: 3744 5432 3744 Size: 1688 6980 SbfI CC_TGCA'GG Cuts at: 6020 6020 Size: 8668 ScaI AGT'ACT Cuts at: 6916 8628 6916 Size: 1712 6956 SfcI C'TryA_G Cuts at: 826 1045 2509 3080 3827 4320 5585 6016 7151 Size: 219 1464 571 747 493 1265 431 1135 Cuts at: 7151 7832 8023 826 Size: 681 191 1471 Fragments arranged by size: 1471 1464 1265 1135 747 681 571 493 431 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 4469 5327 4469 Size: 858 7810 SfoI GGC'GCC Cuts at: 4005 4005 Size: 8668 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8668 SmaI CCC'GGG Cuts at: 1872 3130 3181 3791 4698 5012 5809 5825 5843 Size: 1258 51 610 907 314 797 16 18 Cuts at: 5843 5872 5995 1872 Size: 29 123 4545 Fragments arranged by size: 4545 1258 907 797 610 314 123 51 29 18 16 SmlI C'TyrA_G Cuts at: 820 1017 1943 2027 2189 2225 2246 3659 4676 Size: 197 926 84 162 36 21 1413 1017 Cuts at: 4676 6772 7673 7935 820 Size: 2096 901 262 1553 Fragments arranged by size: 2096 1553 1413 1017 926 901 262 197 162 84 36 21 SnaBI TAC'GTA Cuts at: 493 6459 493 Size: 5966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8668 SphI G_CATG'C Cuts at: 1132 3805 5497 6026 1132 Size: 2673 1692 529 3774 Fragments arranged by size: 3774 2673 1692 529 SrfI GCCC'GGGC Cuts at: 5843 5843 Size: 8668 SspI AAT'ATT Cuts at: 5 52 6592 5 Size: 47 6540 2081 Fragments arranged by size: 6540 2081 47 StuI AGG'CCT Cuts at: 1517 2924 3467 1517 Size: 1407 543 6718 Fragments arranged by size: 6718 1407 543 StyI C'CwwG_G Cuts at: 513 1065 2157 2487 4335 4467 5536 6107 513 Size: 552 1092 330 1848 132 1069 571 3074 Fragments arranged by size: 3074 1848 1092 1069 571 552 330 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3534 6695 6712 6865 7050 7495 325 Size: 1255 1954 3161 17 153 185 445 1498 Fragments arranged by size: 3161 1954 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2550 6914 8626 Size: 274 80 33 51 1443 573 4364 1712 Cuts at: 8626 96 Size: 138 Fragments arranged by size: 4364 1712 1443 573 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2258 2290 8628 8660 2258 Size: 32 6338 32 2266 Fragments arranged by size: 6338 2266 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8668 XbaI T'CTAG_A Cuts at: 6004 6004 Size: 8668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2179 2401 2443 3019 3535 2179 Size: 222 42 576 516 7312 Fragments arranged by size: 7312 576 516 222 42 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8668 XmaI C'CCGG_G Cuts at: 1870 3128 3179 3789 4696 5010 5807 5823 5841 Size: 1258 51 610 907 314 797 16 18 Cuts at: 5841 5870 5993 1870 Size: 29 123 4545 Fragments arranged by size: 4545 1258 907 797 610 314 123 51 29 18 16 XmnI GAAnn'nnTTC Cuts at: 6797 6797 Size: 8668 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3891 6476 276 Size: 53 83 186 936 2357 2585 2468 Fragments arranged by size: 2585 2468 2357 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SwaI