(Circular) (Six-base) MAPSORT of: pFN21AE4294.seq Check: 4754 from: 1 to: 10750 pFN21AE4294 10750 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5886 6896 7731 5886 Size: 1010 835 8905 Fragments arranged by size: 8905 1010 835 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6201 8560 278 Size: 53 83 186 936 4665 2359 2468 Fragments arranged by size: 4665 2468 2359 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2808 8093 1937 Size: 871 5285 4594 Fragments arranged by size: 5285 4594 871 Acc65I G'GTAC_C Cuts at: 5787 8071 5787 Size: 2284 8466 AclI AA'CG_TT Cuts at: 1563 8877 9250 1563 Size: 7314 373 3063 Fragments arranged by size: 7314 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3272 3380 3734 3831 6185 7696 8813 10197 Size: 1640 108 354 97 2354 1511 1117 1384 Cuts at: 10197 10465 1632 Size: 268 1917 Fragments arranged by size: 2354 1917 1640 1511 1384 1117 354 268 108 97 AfeI AGC'GCT Cuts at: 3596 5816 3596 Size: 2220 8530 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10553 AflIII A'CryG_T Cuts at: 1203 7359 9649 10626 1203 Size: 6156 2290 977 1327 Fragments arranged by size: 6156 2290 1327 977 AgeI A'CCGG_T Cuts at: 9581 9581 Size: 10750 AhdI GACnn_n'nnGTC Cuts at: 4684 6166 9479 4684 Size: 1482 3313 5955 Fragments arranged by size: 5955 3313 1482 AleI CACnn'nnGTG Cuts at: 1166 6653 6695 7913 1166 Size: 5487 42 1218 4003 Fragments arranged by size: 5487 4003 1218 42 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2723 1184 Size: 1539 9211 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6461 6493 6461 Size: 32 10718 AlwNI CAG_nnn'CTG Cuts at: 2275 4516 4612 4927 5656 7489 7723 8493 10065 Size: 2241 96 315 729 1833 234 770 1572 Cuts at: 10065 2275 Size: 2960 Fragments arranged by size: 2960 2241 1833 1572 770 729 315 234 96 ApaI G_GGCC'C Cuts at: 3994 5524 5785 3994 Size: 1530 261 8959 Fragments arranged by size: 8959 1530 261 ApaLI G'TGCA_C Cuts at: 7918 8807 9963 7918 Size: 889 1156 8705 Fragments arranged by size: 8705 1156 889 ApoI r'AATT_y Cuts at: 1493 3626 5831 6919 8059 8285 8437 10546 1493 Size: 2133 2205 1088 1140 226 152 2109 1697 Fragments arranged by size: 2205 2133 2109 1697 1140 1088 226 152 AseI AT'TA_AT Cuts at: 160 1032 9304 160 Size: 872 8272 1606 Fragments arranged by size: 8272 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10750 AvaI C'yCGr_G Cuts at: 1870 1943 2214 6118 7854 8075 1870 Size: 73 271 3904 1736 221 4545 Fragments arranged by size: 4545 3904 1736 271 221 73 AvrII C'CTAG_G Cuts at: 6268 6268 Size: 10750 BamHI G'GATC_C Cuts at: 8080 8447 8080 Size: 367 10383 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2210 2496 4715 5025 Size: 325 217 627 315 108 286 2219 310 Cuts at: 5025 5765 5787 5874 6263 6983 7260 7423 7817 Size: 740 22 87 389 720 277 163 394 Cuts at: 7817 7947 7973 8071 9526 618 Size: 130 26 98 1455 1842 Fragments arranged by size: 2219 1842 1455 740 720 627 394 389 325 315 310 286 277 217 163 130 108 98 87 26 22 BanII G_rGCy'C Cuts at: 1391 2358 2853 3732 3765 3994 4044 4823 5274 Size: 967 495 879 33 229 50 779 451 Cuts at: 5274 5524 5785 6012 7191 8069 1391 Size: 250 261 227 1179 878 4072 Fragments arranged by size: 4072 1179 967 879 878 779 495 451 261 250 229 227 50 33 BbeI G_GCGC'C Cuts at: 2214 6267 7821 7977 2214 Size: 4053 1554 156 4987 Fragments arranged by size: 4987 4053 1554 156 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2631 3819 4866 5583 7062 928 Size: 971 732 1188 1047 717 1479 4616 Fragments arranged by size: 4616 1479 1188 1047 971 732 717 BbvCI CC'TCA_GC Cuts at: 5870 5870 Size: 10750 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2843 2877 4483 4517 8941 8975 1903 Size: 34 906 34 1606 34 4424 34 3678 Fragments arranged by size: 4424 3678 1606 906 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6862 8642 9852 6862 Size: 1780 1210 7760 Fragments arranged by size: 7760 1780 1210 BclI T'GATC_A Cuts at: 1548 3927 4848 5112 5428 1548 Size: 2379 921 264 316 6870 Fragments arranged by size: 6870 2379 921 316 264 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2381 4121 4568 Size: 107 122 71 631 605 709 1740 447 Cuts at: 4568 5757 7898 9361 136 Size: 1189 2141 1463 1525 Fragments arranged by size: 2141 1740 1525 1463 1189 709 631 605 447 122 107 71 BglII A'GATC_T Cuts at: 3972 7170 10745 3972 Size: 3198 3575 3977 Fragments arranged by size: 3977 3575 3198 BlpI GC'TnA_GC Cuts at: 3888 6020 8167 3888 Size: 2132 2147 6471 Fragments arranged by size: 6471 2147 2132 Bme1580I G_kGCm'C Cuts at: 1790 2499 3994 5496 5524 5785 6284 6842 6986 Size: 709 1495 1502 28 261 499 558 144 Cuts at: 6986 7324 7481 7922 8811 9967 1790 Size: 338 157 441 889 1156 2573 Fragments arranged by size: 2573 1502 1495 1156 889 709 558 499 441 338 261 157 144 28 BmgBI CAC'GTC Cuts at: 1318 4468 4489 4603 5320 5413 7450 1318 Size: 3150 21 114 717 93 2037 4618 Fragments arranged by size: 4618 3150 2037 717 114 93 21 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5532 6407 7073 Size: 416 53 83 399 24 4115 875 666 Cuts at: 7073 8481 9439 442 Size: 1408 958 1753 Fragments arranged by size: 4115 1753 1408 958 875 666 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10750 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7453 7453 Size: 10750 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 2324 3223 3502 3989 4028 4196 4351 Size: 1007 78 899 279 487 39 168 155 Cuts at: 4351 4649 5723 5738 6026 6398 6752 6858 7187 Size: 298 1074 15 288 372 354 106 329 Cuts at: 7187 7205 7823 7981 8045 9410 1239 Size: 18 618 158 64 1365 2579 Fragments arranged by size: 2579 1365 1074 1007 899 618 487 372 354 329 298 288 279 168 158 155 106 78 64 39 18 15 Bpu10I CC'TnA_GC Cuts at: 3771 4547 5870 3771 Size: 776 1323 8651 Fragments arranged by size: 8651 1323 776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2444 3457 3459 4045 6812 7558 8875 9740 10038 Size: 1013 2 586 2767 746 1317 865 298 Cuts at: 10038 2444 Size: 3156 Fragments arranged by size: 3156 2767 1317 1013 865 746 586 298 2 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2193 7700 7746 9413 882 Size: 617 694 5507 46 1667 2219 Fragments arranged by size: 5507 2219 1667 694 617 46 BsaAI yAC'GTr Cuts at: 493 1204 4165 4473 5491 8541 493 Size: 711 2961 308 1018 3050 2702 Fragments arranged by size: 3050 2961 2702 1018 711 308 BsaBI GATnn'nnATC Cuts at: 3929 5111 7490 10744 3929 Size: 1182 2379 3254 3935 Fragments arranged by size: 3935 3254 2379 1182 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2211 4649 5471 6198 Size: 53 83 186 936 678 2438 822 727 Cuts at: 6198 6264 7818 7974 8557 8939 9574 275 Size: 66 1554 156 583 382 635 1451 Fragments arranged by size: 2438 1554 1451 936 822 727 678 635 583 382 186 156 83 66 53 BsaWI w'CCGG_w Cuts at: 2916 9183 9581 9855 10002 10413 2916 Size: 6267 398 274 147 411 3253 Fragments arranged by size: 6267 3253 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3679 3709 5597 5627 6986 Size: 30 165 30 2276 30 1888 30 1359 Cuts at: 6986 7016 7145 7175 7505 7535 1178 Size: 30 129 30 330 30 4393 Fragments arranged by size: 4393 2276 1888 1359 330 165 129 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2243 4208 6689 7262 7484 7528 8024 Size: 886 171 1965 2481 573 222 44 496 Cuts at: 8024 1186 Size: 3912 Fragments arranged by size: 3912 2481 1965 886 573 496 222 171 44 BseYI C'CCAG_C Cuts at: 2030 2253 2371 2451 2696 2744 3775 3784 4186 Size: 223 118 80 245 48 1031 9 402 Cuts at: 4186 5523 6510 7343 7474 7583 7591 7821 7968 Size: 1337 987 833 131 109 8 230 147 Cuts at: 7968 8014 9953 10449 2030 Size: 46 1939 496 2331 Fragments arranged by size: 2331 1939 1337 1031 987 833 496 402 245 230 223 147 131 118 109 80 48 46 9 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2432 2984 4495 4625 5579 5921 6119 Size: 573 696 552 1511 130 954 342 198 Cuts at: 6119 6378 6494 6746 7246 1163 Size: 259 116 252 500 4667 Fragments arranged by size: 4667 1511 954 696 573 552 500 342 259 252 198 130 116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6536 8200 8961 9989 1540 Size: 275 186 4535 1664 761 1028 2301 Fragments arranged by size: 4535 2301 1664 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2853 3732 3765 3889 4044 5724 6012 6210 6366 Size: 879 33 124 155 1680 288 198 156 Cuts at: 6366 7016 7922 8811 8896 9967 2853 Size: 650 906 889 85 1071 3636 Fragments arranged by size: 3636 1680 1071 906 889 879 650 288 198 156 155 124 85 33 BsmI GAATG_Cn' Cuts at: 8261 8354 8261 Size: 93 10657 BsmBI CGTCTCn'nnnn_ Cuts at: 2084 2084 Size: 10750 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2358 2499 2853 3732 3765 3889 3994 Size: 399 568 141 354 879 33 124 105 Cuts at: 3994 4044 4823 5274 5496 5524 5724 5785 6012 Size: 50 779 451 222 28 200 61 227 Cuts at: 6012 6210 6284 6366 6842 6986 7016 7191 7324 Size: 198 74 82 476 144 30 175 133 Cuts at: 7324 7481 7922 8069 8811 8896 9967 1391 Size: 157 441 147 742 85 1071 2174 Fragments arranged by size: 2174 1071 879 779 742 568 476 451 441 399 354 227 222 200 198 175 157 147 144 141 133 124 105 85 82 74 61 50 33 30 28 BspEI T'CCGG_A Cuts at: 2916 10413 2916 Size: 7497 3253 BspHI T'CATG_A Cuts at: 5673 6498 8639 10369 5673 Size: 825 2141 1730 6054 Fragments arranged by size: 6054 2141 1730 825 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2229 3497 3888 4689 5142 5886 6141 6896 Size: 1385 1268 391 801 453 744 255 755 Cuts at: 6896 7731 8105 844 Size: 835 374 3489 Fragments arranged by size: 3489 1385 1268 835 801 755 744 453 391 374 255 BsrBI CCG'CTC Cuts at: 1394 1823 4353 5602 5992 6019 8637 1394 Size: 429 2530 1249 390 27 2618 3507 Fragments arranged by size: 3507 2618 2530 1249 429 390 27 BsrDI GCAATG_nn' Cuts at: 65 1248 2628 3835 3873 7135 7836 9245 9419 Size: 1183 1380 1207 38 3262 701 1409 174 Cuts at: 9419 65 Size: 1396 Fragments arranged by size: 3262 1409 1396 1380 1207 1183 701 174 38 BsrFI r'CCGG_y Cuts at: 1700 1810 4051 4111 4712 5854 8019 9394 9581 Size: 110 2241 60 601 1142 2165 1375 187 Cuts at: 9581 1700 Size: 2869 Fragments arranged by size: 2869 2241 2165 1375 1142 601 187 110 60 BsrGI T'GTAC_A Cuts at: 96 7461 96 Size: 7365 3385 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10750 BssSI C'ACGA_G Cuts at: 8810 9822 8810 Size: 1012 9738 BstAPI GCAn_nnn'nTGC Cuts at: 794 2419 5491 5944 7876 794 Size: 1625 3072 453 1932 3668 Fragments arranged by size: 3668 3072 1932 1625 453 BstBI TT'CG_AA Cuts at: 3108 9554 3108 Size: 6446 4304 BstEII G'GTnAC_C Cuts at: 1184 2141 1184 Size: 957 9793 BstXI CCAn_nnnn'nTGG Cuts at: 2872 3270 3967 7096 7285 2872 Size: 398 697 3129 189 6337 Fragments arranged by size: 6337 3129 697 398 189 BstYI r'GATC_y Cuts at: 1972 2299 3265 3511 3972 4803 6779 7170 7485 Size: 327 966 246 461 831 1976 391 315 Cuts at: 7485 7737 8080 8447 8832 8849 10301 10387 10399 Size: 252 343 367 385 17 1452 86 12 Cuts at: 10399 10745 1972 Size: 346 1977 Fragments arranged by size: 1977 1976 1452 966 831 461 391 385 367 346 343 327 315 252 246 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4751 4865 7352 7428 4751 Size: 114 2487 76 8073 Fragments arranged by size: 8073 2487 114 76 BtgI C'CryG_G Cuts at: 513 1065 2004 2085 2203 2358 2376 4080 4200 Size: 552 939 81 118 155 18 1704 120 Cuts at: 4200 4254 4744 5014 5391 6651 6972 7089 7206 Size: 54 490 270 377 1260 321 117 117 Cuts at: 7206 7695 7893 513 Size: 489 198 3370 Fragments arranged by size: 3370 1704 1260 939 552 490 489 377 321 270 198 155 120 118 117 117 81 54 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 7030 7426 487 Size: 683 56 5804 396 3811 Fragments arranged by size: 5804 3811 683 396 56 BtsI GCAGTG_nn' Cuts at: 5767 8266 9060 9080 5767 Size: 2499 794 20 7437 Fragments arranged by size: 7437 2499 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10750 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3464 3499 4236 4271 529 Size: 35 2900 35 737 35 7008 Fragments arranged by size: 7008 2900 737 35 35 35 DraI TTT'AAA Cuts at: 8054 8054 Size: 10750 DraIII CAC_nnn'GTG Cuts at: 4035 7944 4035 Size: 3909 6841 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9757 809 Size: 507 8441 1802 Fragments arranged by size: 8441 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2604 4113 4122 Size: 54 1425 50 275 18 774 1509 9 Cuts at: 4122 4203 4833 4929 5592 5688 5805 6897 7410 Size: 81 630 96 663 96 117 1092 513 Cuts at: 7410 7953 8197 9086 10715 8 Size: 543 244 889 1629 43 Fragments arranged by size: 1629 1509 1425 1092 889 774 663 630 543 513 275 244 117 96 96 81 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 8197 1537 Size: 275 6385 4090 Fragments arranged by size: 6385 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3710 4902 5702 8680 1353 Size: 2357 1192 800 2978 3423 Fragments arranged by size: 3423 2978 2357 1192 800 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5138 6434 9337 9711 9857 387 Size: 1064 138 3549 1296 2903 374 146 1280 Fragments arranged by size: 3549 2903 1296 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2851 3730 3763 4042 6010 2851 Size: 879 33 279 1968 7591 Fragments arranged by size: 7591 1968 879 279 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 2324 3223 3272 3380 3502 3734 Size: 393 614 78 899 49 108 122 232 Cuts at: 3734 3831 3989 4028 4196 4351 4649 5723 5738 Size: 97 158 39 168 155 298 1074 15 Cuts at: 5738 6026 6185 6398 6752 6858 7187 7205 7696 Size: 288 159 213 354 106 329 18 491 Cuts at: 7696 7823 7981 8045 8813 9410 10197 10465 1239 Size: 127 158 64 768 597 787 268 1524 Fragments arranged by size: 1524 1074 899 787 768 614 597 491 393 354 329 298 288 268 232 213 168 159 158 158 155 127 122 108 106 97 78 64 49 39 18 15 EcoNI CCTnn'n_nnAGG Cuts at: 2225 4556 5750 6719 7669 7805 2225 Size: 2331 1194 969 950 136 5170 Fragments arranged by size: 5170 2331 1194 969 950 136 EcoO109I rG'GnC_Cy Cuts at: 2218 2438 2655 2902 3857 3938 3990 3991 4156 Size: 220 217 247 955 81 52 1 165 Cuts at: 4156 4243 4678 6298 6568 6712 7339 7566 7765 Size: 87 435 1620 270 144 627 227 199 Cuts at: 7765 2218 Size: 5203 Fragments arranged by size: 5203 1620 955 627 435 270 247 227 220 217 199 165 144 87 81 52 1 EcoRI G'AATT_C Cuts at: 6919 8059 8437 6919 Size: 1140 378 9232 Fragments arranged by size: 9232 1140 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3666 5083 3666 Size: 1417 9333 FspI TGC'GCA Cuts at: 9256 9256 Size: 10750 HaeII r_GCGC'y Cuts at: 1678 2214 3598 4187 4585 5818 5969 6267 6391 Size: 536 1384 589 398 1233 151 298 124 Cuts at: 6391 6497 7821 7977 9897 1678 Size: 106 1324 156 1920 2531 Fragments arranged by size: 2531 1920 1384 1324 1233 589 536 398 298 156 151 124 106 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4670 4702 5597 5629 6072 Size: 32 135 32 3098 32 895 32 443 Cuts at: 6072 6104 6152 6184 6298 6330 6332 6362 6364 Size: 32 48 32 114 32 2 30 2 Cuts at: 6364 6394 6986 7018 7145 7177 7505 7537 7773 Size: 30 592 32 127 32 328 32 236 Cuts at: 7773 7805 9391 9423 9465 9497 1373 Size: 32 1586 32 42 32 2626 Fragments arranged by size: 3098 2626 1586 895 592 443 328 236 135 127 114 48 42 32 32 32 32 32 32 32 32 32 32 32 32 32 30 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 4132 7102 8094 8340 669 Size: 988 281 2194 2970 992 246 3079 Fragments arranged by size: 3079 2970 2194 992 988 281 246 HindIII A'AGCT_T Cuts at: 748 3297 748 Size: 2549 8201 HpaI GTT'AAC Cuts at: 8340 8340 Size: 10750 KasI G'GCGC_C Cuts at: 2210 6263 7817 7973 2210 Size: 4053 1554 156 4987 Fragments arranged by size: 4987 4053 1554 156 KpnI G_GTAC'C Cuts at: 5791 8075 5791 Size: 2284 8466 MfeI C'AATT_G Cuts at: 8349 8349 Size: 10750 MluI A'CGCG_T Cuts at: 10626 10626 Size: 10750 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2958 5340 6150 7692 9864 10048 1305 Size: 1653 2382 810 1542 2172 184 2007 Fragments arranged by size: 2382 2172 2007 1653 1542 810 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4124 4205 4835 4931 10 Size: 54 1425 343 2292 81 630 96 5829 Fragments arranged by size: 5829 2292 1425 630 343 96 81 54 MslI CAynn'nnrTG Cuts at: 518 1166 4407 6182 6587 6653 6695 6722 7094 Size: 648 3241 1775 405 66 42 27 372 Cuts at: 7094 7913 8708 9067 9226 518 Size: 819 795 359 159 2042 Fragments arranged by size: 3241 2042 1775 819 795 648 405 372 359 159 66 42 27 MspA1I CmG'CkG Cuts at: 1606 2066 2205 2257 2455 3457 3784 3802 3892 Size: 460 139 52 198 1002 327 18 90 Cuts at: 3892 4456 4627 4636 5647 6538 6878 6999 7107 Size: 564 171 9 1011 891 340 121 108 Cuts at: 7107 7315 7825 8166 8843 9991 10236 1606 Size: 208 510 341 677 1148 245 2120 Fragments arranged by size: 2120 1148 1011 1002 891 677 564 510 460 341 340 327 245 208 198 171 139 121 108 90 52 18 9 NaeI GCC'GGC Cuts at: 4053 8021 4053 Size: 3968 6782 NarI GG'CG_CC Cuts at: 2211 6264 7818 7974 2211 Size: 4053 1554 156 4987 Fragments arranged by size: 4987 4053 1554 156 NcoI C'CATG_G Cuts at: 513 1065 2004 2085 5391 7089 7206 7695 513 Size: 552 939 81 3306 1698 117 489 3568 Fragments arranged by size: 3568 3306 1698 939 552 489 117 81 NdeI CA'TA_TG Cuts at: 387 387 Size: 10750 NgoMIV G'CCGG_C Cuts at: 4051 8019 4051 Size: 3968 6782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10750 NotI GC'GGCC_GC Cuts at: 8197 8197 Size: 10750 NspI r_CATG'y Cuts at: 1132 1229 2017 2038 5935 6088 6838 7363 7645 Size: 97 788 21 3897 153 750 525 282 Cuts at: 7645 8108 9653 1132 Size: 463 1545 2229 Fragments arranged by size: 3897 2229 1545 788 750 525 463 282 153 97 21 PasI CC'CwG_GG Cuts at: 1348 2802 2898 3270 3403 6076 6634 6743 1348 Size: 1454 96 372 133 2673 558 109 5355 Fragments arranged by size: 5355 2673 1454 558 372 133 109 96 PciI A'CATG_T Cuts at: 7359 9649 7359 Size: 2290 8460 PflMI CCAn_nnn'nTGG Cuts at: 1108 3129 4035 4516 4927 5259 5803 1108 Size: 2021 906 481 411 332 544 6055 Fragments arranged by size: 6055 2021 906 544 481 411 332 PfoI T'CCnGG_A Cuts at: 2755 3040 3599 3756 3966 4731 5700 6680 2755 Size: 285 559 157 210 765 969 980 6825 Fragments arranged by size: 6825 980 969 765 559 285 210 157 PmeI GTTT'AAAC Cuts at: 8054 8054 Size: 10750 PmlI CAC'GTG Cuts at: 4473 4473 Size: 10750 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8819 8851 10332 10364 8819 Size: 32 1481 32 9205 Fragments arranged by size: 9205 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2218 2438 3938 4678 6568 7339 7566 7765 2218 Size: 220 1500 740 1890 771 227 199 5203 Fragments arranged by size: 5203 1890 1500 771 740 227 220 199 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10750 PsiI TTA'TAA Cuts at: 8320 8320 Size: 10750 PspOMI G'GGCC_C Cuts at: 3990 5520 5781 3990 Size: 1530 261 8959 Fragments arranged by size: 8959 1530 261 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4907 4939 4907 Size: 32 10718 PstI C_TGCA'G Cuts at: 830 2152 2447 3494 3841 4557 4686 4800 5139 Size: 1322 295 1047 347 716 129 114 339 Cuts at: 5139 5652 5949 6492 7178 7728 8102 830 Size: 513 297 543 686 550 374 3478 Fragments arranged by size: 3478 1322 1047 716 686 550 543 513 374 347 339 297 295 129 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10750 PvuII CAG'CTG Cuts at: 2066 2257 3457 3784 3802 3892 4456 4627 4636 Size: 191 1200 327 18 90 564 171 9 Cuts at: 4636 6878 6999 7315 2066 Size: 2242 121 316 5501 Fragments arranged by size: 5501 2242 1200 564 327 316 191 171 121 90 18 9 SacI G_AGCT'C Cuts at: 2853 3732 3765 4044 6012 2853 Size: 879 33 279 1968 7591 Fragments arranged by size: 7591 1968 879 279 33 SacII CC_GC'GG Cuts at: 2206 2206 Size: 10750 SalI G'TCGA_C Cuts at: 1936 8092 1936 Size: 6156 4594 SanDI GG'GwC_CC Cuts at: 3938 7339 7765 3938 Size: 3401 426 6923 Fragments arranged by size: 6923 3401 426 SapI GCTCTTCn'nnn_ Cuts at: 3710 4902 5702 3710 Size: 1192 800 8758 Fragments arranged by size: 8758 1192 800 SbfI CC_TGCA'GG Cuts at: 3494 4557 7728 8102 3494 Size: 1063 3171 374 6142 Fragments arranged by size: 6142 3171 1063 374 ScaI AGT'ACT Cuts at: 2720 4916 5564 8998 10710 2720 Size: 2196 648 3434 1712 2760 Fragments arranged by size: 3434 2760 2196 1712 648 SexAI A'CCwGG_T Cuts at: 2434 6927 2434 Size: 4493 6257 SfcI C'TryA_G Cuts at: 826 1045 2148 2443 3149 3490 3837 4553 4682 Size: 219 1103 295 706 341 347 716 129 Cuts at: 4682 4796 5135 5221 5648 5945 6488 7174 7724 Size: 114 339 86 427 297 543 686 550 Cuts at: 7724 8098 9233 9914 10105 826 Size: 374 1135 681 191 1471 Fragments arranged by size: 1471 1135 1103 716 706 686 681 550 543 427 374 347 341 339 297 295 219 191 129 114 86 SfiI GGCCn_nnn'nGGCC Cuts at: 4121 4121 Size: 10750 SfoI GGC'GCC Cuts at: 2212 6265 7819 7975 2212 Size: 4053 1554 156 4987 Fragments arranged by size: 4987 4053 1554 156 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10750 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10750 SmaI CCC'GGG Cuts at: 1872 2216 8077 1872 Size: 344 5861 4545 Fragments arranged by size: 5861 4545 344 SmlI C'TyrA_G Cuts at: 820 1017 1943 2423 3436 3474 4060 6827 7573 Size: 197 926 480 1013 38 586 2767 746 Cuts at: 7573 8854 9755 10017 820 Size: 1281 901 262 1553 Fragments arranged by size: 2767 1553 1281 1013 926 901 746 586 480 262 197 38 SnaBI TAC'GTA Cuts at: 493 8541 493 Size: 8048 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10750 SphI G_CATG'C Cuts at: 1132 5935 8108 1132 Size: 4803 2173 3774 Fragments arranged by size: 4803 3774 2173 SspI AAT'ATT Cuts at: 5 52 2637 4813 8674 5 Size: 47 2585 2176 3861 2081 Fragments arranged by size: 3861 2585 2176 2081 47 StuI AGG'CCT Cuts at: 1517 2234 6313 6863 1517 Size: 717 4079 550 5404 Fragments arranged by size: 5404 4079 717 550 StyI C'CwwG_G Cuts at: 513 1065 2004 2085 2478 4068 4239 5391 6268 Size: 552 939 81 393 1590 171 1152 877 Cuts at: 6268 6435 6864 7089 7206 7284 7377 7695 8189 Size: 167 429 225 117 78 93 318 494 Cuts at: 8189 513 Size: 3074 Fragments arranged by size: 3074 1590 1152 939 877 552 494 429 393 318 225 171 167 117 93 81 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4815 5027 5693 6107 8777 8794 8947 Size: 1255 3235 212 666 414 2670 17 153 Cuts at: 8947 9132 9577 325 Size: 185 445 1498 Fragments arranged by size: 3235 2670 1498 1255 666 445 414 212 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2037 2718 4914 Size: 274 80 33 51 1443 60 681 2196 Cuts at: 4914 5562 6645 7461 8996 10708 96 Size: 648 1083 816 1535 1712 138 Fragments arranged by size: 2196 1712 1535 1443 1083 816 681 648 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3679 3711 5402 5434 10710 10742 3679 Size: 32 1691 32 5276 32 3687 Fragments arranged by size: 5276 3687 1691 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3689 5411 5582 1316 Size: 2373 1722 171 6484 Fragments arranged by size: 6484 2373 1722 171 XbaI T'CTAG_A Cuts at: 8086 8086 Size: 10750 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3038 3310 4004 4052 4303 5698 5759 3038 Size: 272 694 48 251 1395 61 8029 Fragments arranged by size: 8029 1395 694 272 251 61 48 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10750 XmaI C'CCGG_G Cuts at: 1870 2214 8075 1870 Size: 344 5861 4545 Fragments arranged by size: 5861 4545 344 XmnI GAAnn'nnTTC Cuts at: 2570 5903 8879 2570 Size: 3333 2976 4441 Fragments arranged by size: 4441 3333 2976 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6199 8558 276 Size: 53 83 186 936 4665 2359 2468 Fragments arranged by size: 4665 2468 2359 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspXI RsrII SrfI SwaI