(Circular) (Six-base) MAPSORT of: pFN21AE3717.seq Check: 7452 from: 1 to: 11851 pFN21AE3717 11851 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2921 8723 9661 278 Size: 53 83 186 936 1385 5802 938 2468 Fragments arranged by size: 5802 2468 1385 938 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2709 4559 5208 5629 5911 8309 8766 9194 Size: 772 1850 649 421 282 2398 457 428 Cuts at: 9194 1937 Size: 4594 Fragments arranged by size: 4594 2398 1850 772 649 457 428 421 282 Acc65I G'GTAC_C Cuts at: 3158 9172 3158 Size: 6014 5837 AclI AA'CG_TT Cuts at: 1563 9978 10351 1563 Size: 8415 373 3063 Fragments arranged by size: 8415 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2813 2993 3146 3375 3846 3852 3912 5217 Size: 1181 180 153 229 471 6 60 1305 Cuts at: 5217 6162 6171 6336 6357 6933 7743 8463 9914 Size: 945 9 165 21 576 810 720 1451 Cuts at: 9914 11298 11566 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1451 1384 1305 1181 945 810 720 576 471 268 229 180 165 153 60 21 9 6 AflII C'TTAA_G Cuts at: 820 1017 6022 8558 820 Size: 197 5005 2536 4113 Fragments arranged by size: 5005 4113 2536 197 AflIII A'CryG_T Cuts at: 1203 10750 11727 1203 Size: 9547 977 1327 Fragments arranged by size: 9547 1327 977 AgeI A'CCGG_T Cuts at: 10682 10682 Size: 11851 AhdI GACnn_n'nnGTC Cuts at: 8194 10580 8194 Size: 2386 9465 AleI CACnn'nnGTG Cuts at: 1166 4464 4985 6176 6455 1166 Size: 3298 521 1191 279 6562 Fragments arranged by size: 6562 3298 1191 521 279 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 11851 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4407 4439 5028 5060 5666 5698 7402 7434 4407 Size: 32 589 32 606 32 1704 32 8824 Fragments arranged by size: 8824 1704 606 589 32 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2423 4621 5852 6074 6380 7559 7814 8272 9594 Size: 2198 1231 222 306 1179 255 458 1322 Cuts at: 9594 11166 2423 Size: 1572 3108 Fragments arranged by size: 3108 2198 1572 1322 1231 1179 458 306 255 222 ApaI G_GGCC'C Cuts at: 2456 8779 8840 2456 Size: 6323 61 5467 Fragments arranged by size: 6323 5467 61 ApaLI G'TGCA_C Cuts at: 5214 9908 11064 5214 Size: 4694 1156 6001 Fragments arranged by size: 6001 4694 1156 ApoI r'AATT_y Cuts at: 1493 5367 5447 9085 9160 9386 9538 11647 1493 Size: 3874 80 3638 75 226 152 2109 1697 Fragments arranged by size: 3874 3638 2109 1697 226 152 80 75 AseI AT'TA_AT Cuts at: 160 1032 3695 10405 160 Size: 872 2663 6710 1606 Fragments arranged by size: 6710 2663 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11851 AvaI C'yCGr_G Cuts at: 1870 1943 4005 4660 5471 8778 9176 1870 Size: 73 2062 655 811 3307 398 4545 Fragments arranged by size: 4545 3307 2062 811 655 398 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3911 3944 4847 4880 3911 Size: 33 903 33 10882 Fragments arranged by size: 10882 903 33 33 BamHI G'GATC_C Cuts at: 3983 9181 9548 3983 Size: 5198 367 6286 Fragments arranged by size: 6286 5198 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2015 2169 2985 3158 7607 Size: 325 217 627 228 154 816 173 4449 Cuts at: 7607 8326 8609 9172 10627 618 Size: 719 283 563 1455 1842 Fragments arranged by size: 4449 1842 1455 816 719 627 563 325 283 228 217 173 154 BanII G_rGCy'C Cuts at: 1391 2361 2456 5873 7018 8117 8779 8840 9170 Size: 970 95 3417 1145 1099 662 61 330 Cuts at: 9170 1391 Size: 4072 Fragments arranged by size: 4072 3417 1145 1099 970 662 330 95 61 BbeI G_GCGC'C Cuts at: 2019 2173 2019 Size: 154 11697 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2819 2999 3985 5697 8028 8220 8380 Size: 971 920 180 986 1712 2331 192 160 Cuts at: 8380 928 Size: 4399 Fragments arranged by size: 4399 2331 1712 986 971 920 192 180 160 BbvCI CC'TCA_GC Cuts at: 4221 4800 4221 Size: 579 11272 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 10042 10076 1903 Size: 34 8105 34 3678 Fragments arranged by size: 8105 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6357 6812 9743 10953 6357 Size: 455 2931 1210 7255 Fragments arranged by size: 7255 2931 1210 455 BclI T'GATC_A Cuts at: 1548 3652 5611 7428 1548 Size: 2104 1959 1817 5971 Fragments arranged by size: 5971 2104 1959 1817 BfrBI ATG'CAT Cuts at: 3381 3381 Size: 11851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3087 6700 7799 Size: 107 122 71 631 605 1415 3613 1099 Cuts at: 7799 10462 136 Size: 2663 1525 Fragments arranged by size: 3613 2663 1525 1415 1099 631 605 122 107 71 BglII A'GATC_T Cuts at: 3294 11846 3294 Size: 8552 3299 BlpI GC'TnA_GC Cuts at: 6530 7017 8111 8703 9268 6530 Size: 487 1094 592 565 9113 Fragments arranged by size: 9113 1094 592 565 487 Bme1580I G_kGCm'C Cuts at: 1790 2456 5218 5944 6138 7612 7780 8331 8738 Size: 666 2762 726 194 1474 168 551 407 Cuts at: 8738 8779 8823 8840 8969 9912 11068 1790 Size: 41 44 17 129 943 1156 2573 Fragments arranged by size: 2762 2573 1474 1156 943 726 666 551 407 194 168 129 44 41 17 BmgBI CAC'GTC Cuts at: 1318 5939 1318 Size: 4621 7230 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2129 2492 3933 Size: 416 53 83 399 24 712 363 1441 Cuts at: 3933 4421 5823 7208 7360 7824 8327 9582 10540 Size: 488 1402 1385 152 464 503 1255 958 Cuts at: 10540 442 Size: 1753 Fragments arranged by size: 1753 1441 1402 1385 1255 958 712 503 488 464 416 399 363 152 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3834 1056 Size: 2778 9073 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2055 4350 5349 2055 Size: 2295 999 8557 Fragments arranged by size: 8557 2295 999 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2074 2303 2400 2645 4282 4839 6057 6177 Size: 835 229 97 245 1637 557 1218 120 Cuts at: 6177 6402 6684 7032 7058 8557 8622 10511 1239 Size: 225 282 348 26 1499 65 1889 2579 Fragments arranged by size: 2579 1889 1637 1499 1218 835 557 348 282 245 229 225 120 97 65 26 Bpu10I CC'TnA_GC Cuts at: 4221 4800 6411 4221 Size: 579 1611 9661 Fragments arranged by size: 9661 1611 579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2651 3263 4120 4159 4320 4341 5733 7503 9976 Size: 612 857 39 161 21 1392 1770 2473 Cuts at: 9976 10841 11139 2651 Size: 865 298 3363 Fragments arranged by size: 3363 2473 1770 1392 865 857 612 298 161 39 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2366 2621 3099 5385 6200 6990 7034 Size: 617 867 255 478 2286 815 790 44 Cuts at: 7034 10514 882 Size: 3480 2219 Fragments arranged by size: 3480 2286 2219 867 815 790 617 478 255 44 BsaAI yAC'GTr Cuts at: 493 1204 3518 9642 493 Size: 711 2314 6124 2702 Fragments arranged by size: 6124 2702 2314 711 BsaBI GATnn'nnATC Cuts at: 2963 3912 8250 11845 2963 Size: 949 4338 3595 2969 Fragments arranged by size: 4338 3595 2969 949 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2016 2170 2918 8720 Size: 53 83 186 936 483 154 748 5802 Cuts at: 8720 9658 10040 10675 275 Size: 938 382 635 1451 Fragments arranged by size: 5802 1451 938 936 748 635 483 382 186 154 83 53 BsaWI w'CCGG_w Cuts at: 2353 7282 8084 10284 10682 10956 11103 11514 2353 Size: 4929 802 2200 398 274 147 411 2690 Fragments arranged by size: 4929 2690 2200 802 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3013 3043 3447 3477 4237 Size: 30 165 30 1610 30 404 30 760 Cuts at: 4237 4267 4479 4509 5028 5058 5382 5412 5956 Size: 30 212 30 519 30 324 30 544 Cuts at: 5956 5986 6532 6562 6657 6687 7264 7294 7548 Size: 30 546 30 95 30 577 30 254 Cuts at: 7548 7578 1178 Size: 30 5451 Fragments arranged by size: 5451 1610 760 577 546 544 519 404 324 254 212 165 95 30 30 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2546 3206 3749 4245 4539 5489 7929 Size: 988 372 660 543 496 294 950 2440 Cuts at: 7929 8161 1186 Size: 232 4876 Fragments arranged by size: 4876 2440 988 950 660 543 496 372 294 232 BseYI C'CCAG_C Cuts at: 2313 2318 2406 2474 2505 4389 5055 5097 5181 Size: 5 88 68 31 1884 666 42 84 Cuts at: 5181 7325 7371 8350 8442 8493 9031 11054 11550 Size: 2144 46 979 92 51 538 2023 496 Cuts at: 11550 2313 Size: 2614 Fragments arranged by size: 2614 2144 2023 1884 979 666 538 496 92 88 84 68 51 46 42 31 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2262 3273 4289 4299 5009 6624 8493 Size: 573 526 1011 1016 10 710 1615 1869 Cuts at: 8493 8847 1163 Size: 354 4167 Fragments arranged by size: 4167 1869 1615 1016 1011 710 573 526 354 10 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2133 2211 9301 10062 11090 1540 Size: 275 186 132 78 7090 761 1028 2301 Fragments arranged by size: 7090 2301 1028 761 275 186 132 78 BsiHKAI G_wGCw'C Cuts at: 2235 2350 4471 5218 7018 7064 8117 9912 9997 Size: 115 2121 747 1800 46 1053 1795 85 Cuts at: 9997 11068 2235 Size: 1071 3018 Fragments arranged by size: 3018 2121 1800 1795 1071 1053 747 115 85 46 BsmI GAATG_Cn' Cuts at: 5727 9362 9455 5727 Size: 3635 93 8123 Fragments arranged by size: 8123 3635 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2350 2361 2456 4471 5218 5873 Size: 399 445 115 11 95 2015 747 655 Cuts at: 5873 5944 6138 7018 7064 7612 7780 8117 8331 Size: 71 194 880 46 548 168 337 214 Cuts at: 8331 8738 8779 8823 8840 8969 9170 9912 9997 Size: 407 41 44 17 129 201 742 85 Cuts at: 9997 11068 1391 Size: 1071 2174 Fragments arranged by size: 2174 2015 1071 880 747 742 655 548 445 407 399 337 214 201 194 168 129 115 95 85 71 46 44 41 17 11 BspEI T'CCGG_A Cuts at: 11514 11514 Size: 11851 BspHI T'CATG_A Cuts at: 9740 11470 9740 Size: 1730 10121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3769 6268 7304 8280 9147 9206 844 Size: 2925 2499 1036 976 867 59 3489 Fragments arranged by size: 3489 2925 2499 1036 976 867 59 BsrBI CCG'CTC Cuts at: 1394 1823 4495 9738 1394 Size: 429 2672 5243 3507 Fragments arranged by size: 5243 3507 2672 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5257 6030 10346 10520 65 Size: 1183 4009 773 4316 174 1396 Fragments arranged by size: 4316 4009 1396 1183 773 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2065 2147 2542 3077 3836 10495 10682 Size: 110 255 82 395 535 759 6659 187 Cuts at: 10682 1700 Size: 2869 Fragments arranged by size: 6659 2869 759 535 395 255 187 110 82 BsrGI T'GTAC_A Cuts at: 96 6841 96 Size: 6745 5106 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11851 BssSI C'ACGA_G Cuts at: 9911 10923 9911 Size: 1012 10839 BstAPI GCAn_nnn'nTGC Cuts at: 794 7607 794 Size: 6813 5038 BstBI TT'CG_AA Cuts at: 10655 10655 Size: 11851 BstEII G'GTnAC_C Cuts at: 1184 8453 1184 Size: 7269 4582 BstXI CCAn_nnnn'nTGG Cuts at: 2281 2638 4597 8513 2281 Size: 357 1959 3916 5619 Fragments arranged by size: 5619 3916 1959 357 BstYI r'GATC_y Cuts at: 1972 2994 3294 3983 4422 7387 9181 9548 9933 Size: 1022 300 689 439 2965 1794 367 385 Cuts at: 9933 9950 11402 11488 11500 11846 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2965 1977 1794 1452 1022 689 439 385 367 346 300 86 17 12 BstZ17I GTA'TAC Cuts at: 8310 8310 Size: 11851 Bsu36I CC'TnA_GG Cuts at: 3900 3900 Size: 11851 BtgI C'CryG_G Cuts at: 513 1065 2106 2127 2200 2328 2637 2736 3663 Size: 552 1041 21 73 128 309 99 927 Cuts at: 3663 4117 4767 6567 7461 513 Size: 454 650 1800 894 4903 Fragments arranged by size: 4903 1800 1041 927 894 650 552 454 309 128 99 73 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 11112 Fragments arranged by size: 11112 683 56 BtsI GCAGTG_nn' Cuts at: 2274 9367 10161 10181 2274 Size: 7093 794 20 3944 Fragments arranged by size: 7093 3944 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11851 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4332 4367 7848 7883 529 Size: 35 3768 35 3481 35 4497 Fragments arranged by size: 4497 3768 3481 35 35 35 DraI TTT'AAA Cuts at: 2800 2953 9155 2800 Size: 153 6202 5496 Fragments arranged by size: 6202 5496 153 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 10858 809 Size: 507 9542 1802 Fragments arranged by size: 9542 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2208 2656 Size: 54 1425 50 275 18 300 78 448 Cuts at: 2656 9049 9298 10187 11816 8 Size: 6393 249 889 1629 43 Fragments arranged by size: 6393 1629 1425 889 448 300 275 249 78 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2130 2208 9298 1537 Size: 275 318 78 7090 4090 Fragments arranged by size: 7090 4090 318 275 78 EarI CTCTTCn'nnn_ Cuts at: 1353 2364 2613 3753 4897 6138 6658 7762 9781 Size: 1011 249 1140 1144 1241 520 1104 2019 Cuts at: 9781 1353 Size: 3423 Fragments arranged by size: 3423 2019 1241 1144 1140 1104 1011 520 249 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2234 10438 10812 10958 387 Size: 1064 138 645 8204 374 146 1280 Fragments arranged by size: 8204 1280 1064 645 374 146 138 EcoICRI GAG'CTC Cuts at: 7016 8115 7016 Size: 1099 10752 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2074 2303 2400 2645 2813 2993 3146 Size: 393 442 229 97 245 168 180 153 Cuts at: 3146 3375 3846 3852 3912 4282 4839 5217 6057 Size: 229 471 6 60 370 557 378 840 Cuts at: 6057 6162 6171 6177 6336 6357 6402 6684 6933 Size: 105 9 6 159 21 45 282 249 Cuts at: 6933 7032 7058 7743 8463 8557 8622 9914 10511 Size: 99 26 685 720 94 65 1292 597 Cuts at: 10511 11298 11566 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1292 840 787 720 685 597 557 471 442 393 378 370 282 268 249 245 229 229 180 168 159 153 105 99 97 94 65 60 45 26 21 9 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 6666 7347 6666 Size: 681 11170 EcoO109I rG'GnC_Cy Cuts at: 2453 5957 6407 6764 7352 7532 8269 8836 8837 Size: 3504 450 357 588 180 737 567 1 Cuts at: 8837 2453 Size: 5467 Fragments arranged by size: 5467 3504 737 588 567 450 357 180 1 EcoRI G'AATT_C Cuts at: 9160 9538 9160 Size: 378 11473 EcoRV GAT'ATC Cuts at: 2614 8203 2614 Size: 5589 6262 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6525 7985 6525 Size: 1460 10391 FspI TGC'GCA Cuts at: 10357 10357 Size: 11851 HaeII r_GCGC'y Cuts at: 1678 2019 2173 2574 9009 10998 1678 Size: 341 154 401 6435 1989 2531 Fragments arranged by size: 6435 2531 1989 401 341 154 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3286 3318 3396 3428 3674 Size: 32 135 32 1714 32 78 32 246 Cuts at: 3674 3706 3975 4007 4147 4179 4237 4269 4479 Size: 32 269 32 140 32 58 32 210 Cuts at: 4479 4511 5513 5545 6584 6616 7631 7663 7790 Size: 32 1002 32 1039 32 1015 32 127 Cuts at: 7790 7822 8180 8212 10492 10524 10566 10598 1373 Size: 32 358 32 2280 32 42 32 2626 Fragments arranged by size: 2626 2280 1714 1039 1015 1002 358 269 246 210 140 135 127 78 58 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3214 3970 9195 9441 669 Size: 988 281 1276 756 5225 246 3079 Fragments arranged by size: 5225 3079 1276 988 756 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 11851 HpaI GTT'AAC Cuts at: 9441 9441 Size: 11851 KasI G'GCGC_C Cuts at: 2015 2169 2015 Size: 154 11697 KpnI G_GTAC'C Cuts at: 3162 9176 3162 Size: 6014 5837 MfeI C'AATT_G Cuts at: 7400 8770 9450 7400 Size: 1370 680 9801 Fragments arranged by size: 9801 1370 680 MluI A'CGCG_T Cuts at: 11727 11727 Size: 11851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4718 4920 7496 7813 9082 9159 10965 11149 Size: 3413 202 2576 317 1269 77 1806 184 Cuts at: 11149 1305 Size: 2007 Fragments arranged by size: 3413 2576 2007 1806 1269 317 202 184 77 MscI TGG'CCA Cuts at: 10 64 1489 1832 9051 10 Size: 54 1425 343 7219 2810 Fragments arranged by size: 7219 2810 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4464 4595 4985 5223 6176 6455 8025 Size: 648 3298 131 390 238 953 279 1570 Cuts at: 8025 8885 9809 10168 10327 518 Size: 860 924 359 159 2042 Fragments arranged by size: 3298 2042 1570 953 924 860 648 390 359 279 238 159 131 MspA1I CmG'CkG Cuts at: 1606 2129 2330 2423 2559 3718 4367 4604 4652 Size: 523 201 93 136 1159 649 237 48 Cuts at: 4652 4720 6422 6517 7336 8110 8354 8545 9267 Size: 68 1702 95 819 774 244 191 722 Cuts at: 9267 9944 11092 11337 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1702 1159 1148 819 774 722 677 649 523 245 244 237 201 191 136 95 93 68 48 NaeI GCC'GGC Cuts at: 2067 2544 2067 Size: 477 11374 NarI GG'CG_CC Cuts at: 2016 2170 2016 Size: 154 11697 NcoI C'CATG_G Cuts at: 513 1065 3663 4117 4767 7461 513 Size: 552 2598 454 650 2694 4903 Fragments arranged by size: 4903 2694 2598 650 552 454 NdeI CA'TA_TG Cuts at: 387 4033 387 Size: 3646 8205 NgoMIV G'CCGG_C Cuts at: 2065 2542 2065 Size: 477 11374 NheI G'CTAG_C Cuts at: 1052 3830 1052 Size: 2778 9073 NotI GC'GGCC_GC Cuts at: 2130 2208 9298 2130 Size: 78 7090 4683 Fragments arranged by size: 7090 4683 78 NsiI A_TGCA'T Cuts at: 3383 3383 Size: 11851 NspI r_CATG'y Cuts at: 1132 1229 3241 7025 9209 10754 1132 Size: 97 2012 3784 2184 1545 2229 Fragments arranged by size: 3784 2229 2184 2012 1545 97 PacI TTA_AT'TAA Cuts at: 3699 3699 Size: 11851 PasI CC'CwG_GG Cuts at: 1348 2432 4076 1348 Size: 1084 1644 9123 Fragments arranged by size: 9123 1644 1084 PciI A'CATG_T Cuts at: 10750 10750 Size: 11851 PflMI CCAn_nnn'nTGG Cuts at: 1108 2185 9064 1108 Size: 1077 6879 3895 Fragments arranged by size: 6879 3895 1077 PfoI T'CCnGG_A Cuts at: 6661 6943 7348 6661 Size: 282 405 11164 Fragments arranged by size: 11164 405 282 PmeI GTTT'AAAC Cuts at: 9155 9155 Size: 11851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5028 5060 7486 7518 9920 9952 11433 11465 5028 Size: 32 2426 32 2402 32 1481 32 5414 Fragments arranged by size: 5414 2426 2402 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5957 6407 6764 7352 8269 5957 Size: 450 357 588 917 9539 Fragments arranged by size: 9539 917 588 450 357 PshAI GACnn'nnGTC Cuts at: 1613 3111 3975 1613 Size: 1498 864 9489 Fragments arranged by size: 9489 1498 864 PsiI TTA'TAA Cuts at: 9421 9421 Size: 11851 PspOMI G'GGCC_C Cuts at: 2452 8775 8836 2452 Size: 6323 61 5467 Fragments arranged by size: 6323 5467 61 PstI C_TGCA'G Cuts at: 830 2763 4707 5149 6265 6385 8277 8926 9203 Size: 1933 1944 442 1116 120 1892 649 277 Cuts at: 9203 830 Size: 3478 Fragments arranged by size: 3478 1944 1933 1892 1116 649 442 277 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11851 PvuII CAG'CTG Cuts at: 4367 4604 4652 4720 6422 6517 7336 8110 8354 Size: 237 48 68 1702 95 819 774 244 Cuts at: 8354 8545 4367 Size: 191 7673 Fragments arranged by size: 7673 1702 819 774 244 237 191 95 68 48 SacI G_AGCT'C Cuts at: 7018 8117 7018 Size: 1099 10752 SacII CC_GC'GG Cuts at: 2130 2331 2130 Size: 201 11650 SalI G'TCGA_C Cuts at: 1936 9193 1936 Size: 7257 4594 SapI GCTCTTCn'nnn_ Cuts at: 2613 2613 Size: 11851 SbfI CC_TGCA'GG Cuts at: 9203 9203 Size: 11851 ScaI AGT'ACT Cuts at: 4956 5119 6228 6394 6541 7129 10099 11811 4956 Size: 163 1109 166 147 588 2970 1712 4996 Fragments arranged by size: 4996 2970 1712 1109 588 166 163 147 SfcI C'TryA_G Cuts at: 826 1045 2759 3923 4062 4703 4923 5145 5205 Size: 219 1714 1164 139 641 220 222 60 Cuts at: 5205 5859 6261 6381 6423 7854 8273 8922 9199 Size: 654 402 120 42 1431 419 649 277 Cuts at: 9199 10334 11015 11206 826 Size: 1135 681 191 1471 Fragments arranged by size: 1714 1471 1431 1164 1135 681 654 649 641 419 402 277 222 220 219 191 139 120 60 42 SfoI GGC'GCC Cuts at: 2017 2171 2017 Size: 154 11697 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11851 SgrAI Cr'CCGG_yG Cuts at: 1700 2542 1700 Size: 842 11009 SmaI CCC'GGG Cuts at: 1872 4662 9178 1872 Size: 2790 4516 4545 Fragments arranged by size: 4545 4516 2790 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 3242 4005 4135 4174 4335 Size: 197 926 723 576 763 130 39 161 Cuts at: 4335 4356 5748 6022 7518 8558 9955 10856 11118 Size: 21 1392 274 1496 1040 1397 901 262 Cuts at: 11118 820 Size: 1553 Fragments arranged by size: 1553 1496 1397 1392 1040 926 901 763 723 576 274 262 197 161 130 39 21 SnaBI TAC'GTA Cuts at: 493 3518 9642 493 Size: 3025 6124 2702 Fragments arranged by size: 6124 3025 2702 SpeI A'CTAG_T Cuts at: 152 7048 7547 152 Size: 6896 499 4456 Fragments arranged by size: 6896 4456 499 SphI G_CATG'C Cuts at: 1132 3241 7025 9209 1132 Size: 2109 3784 2184 3774 Fragments arranged by size: 3784 3774 2184 2109 SspI AAT'ATT Cuts at: 5 52 2842 4882 9775 5 Size: 47 2790 2040 4893 2081 Fragments arranged by size: 4893 2790 2081 2040 47 StuI AGG'CCT Cuts at: 1517 5921 1517 Size: 4404 7447 StyI C'CwwG_G Cuts at: 513 1065 2574 2631 3663 4117 4408 4767 4786 Size: 552 1509 57 1032 454 291 359 19 Cuts at: 4786 6810 7461 7713 7827 8424 9290 513 Size: 2024 651 252 114 597 866 3074 Fragments arranged by size: 3074 2024 1509 1032 866 651 597 552 454 359 291 252 114 57 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2171 3101 4752 5015 5409 8891 9878 Size: 1255 591 930 1651 263 394 3482 987 Cuts at: 9878 9895 10048 10233 10678 325 Size: 17 153 185 445 1498 Fragments arranged by size: 3482 1651 1498 1255 987 930 591 445 394 263 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2163 4954 5117 Size: 274 80 33 51 1443 186 2791 163 Cuts at: 5117 6226 6335 6392 6539 6611 6841 7127 10097 Size: 1109 109 57 147 72 230 286 2970 Cuts at: 10097 11809 96 Size: 1712 138 Fragments arranged by size: 2970 2791 1712 1443 1109 286 274 230 186 163 147 138 109 80 72 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5956 5988 6285 6317 6657 6689 7062 7094 11811 Size: 32 297 32 340 32 373 32 4717 Cuts at: 11811 11843 5956 Size: 32 5964 Fragments arranged by size: 5964 4717 373 340 297 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11851 XbaI T'CTAG_A Cuts at: 6967 7381 9187 6967 Size: 414 1806 9631 Fragments arranged by size: 9631 1806 414 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6175 6175 Size: 11851 XhoI C'TCGA_G Cuts at: 1943 4005 1943 Size: 2062 9789 XmaI C'CCGG_G Cuts at: 1870 4660 9176 1870 Size: 2790 4516 4545 Fragments arranged by size: 4545 4516 2790 XmnI GAAnn'nnTTC Cuts at: 8419 9980 8419 Size: 1561 10290 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2919 8721 9659 276 Size: 53 83 186 936 1385 5802 938 2468 Fragments arranged by size: 5802 2468 1385 938 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BsiWI BsmBI DraIII FseI FspAI NruI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI