Restriction map for
pFN21AE2571
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccI AccIII AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I AspEI AviII AvrII
BamHI BbeI BbrPI BcgI BclI
BfrI BlnI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoRI EcoT22I
FbaI FseI FspI HindIII KasI
KspI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
PvuI PvuII RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 2844 GGTACC G^GTACC
AccII 173 CGCG CG^CG
AflIII 1067 ACATGT A^C[GA][CT]GT
AocI 339 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2832 AGCGCT AGC^GCT
ApaI 459 GGGCCC GGGCC^C
Asp718I 2844 GGTACC G^GTACC
AspI 1138 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2656 GCTGAGG CCTCAGC(-5/-2)
BglI 3917 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 451 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 451 GCTGAGC GC^T[ACGT]AGC
BsaAI 2441 CACGTA [CT]AC^GT[GA]
BsaWI 1217 ACCGGA [AT]^CCGG[AT]
BsmBI 2129 GAGACG CGTCTC(1/5)
BsmI 2780 GAATGC GAATGC(1/-1)
BspHI 377 TCATGA T^CATGA
BspLU11I 1067 ACATGT A^CATGT
BsrBI 2161 GAGCGG CCGCTC(-3/-3)
BssSI 1993 CACGAG CACGAG(-5/-1)
BstUI 173 CGCG CG^CG
Bsu36I 339 CCTGAGG CC^T[ACGT]AGG
CelII 451 GCTGAGC GC^T[ACGT]AGC
DraI 2148 TTTAAA TTT^AAA
DraIII 983 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2832 AGCGCT AGC^GCT
Eco81I 339 CCTGAGG CC^T[ACGT]AGG
EcoRV 2485 GATATC GAT^ATC
HpaI 195 GTTAAC GTT^AAC
KpnI 2844 GGTACC GGTAC^C
MspA1I 3002 CAGCGG C[AC]G^C[GT]G
MvnI 173 CGCG CG^CG
NspI 1067 ACATGT [GA]CATG^[CT]
PaeR7I 190 CTCGAG C^TCGAG
PflFI 1138 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 614 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1671 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 377 TCATGA T^CATGA
SapI 1153 GCTCTTC GCTCTTC(1/4)
SspI 1175 AATATT AAT^ATT
StuI 2021 AGGCCT AGG^CCT
Tth111I 1138 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2823 TCTAGA T^CTAGA
XhoI 190 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AclI 2193 AACGTT AA^CGTT
2675 AACGTT
AcyI 223 GGCGTC G[GA]^CG[CT]C
291 GGCGTC
AlwI 678 GGATC GGATC(4/5)
1377 GGATC
AlwNI 424 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1383 CAGCAACTG
BanI 331 GGCACC G^G[CT][GA]CC
2844 GGTACC
BcnI 3227 CCCGG CC^[GC]GG
3944 CCGGG
BglII 710 AGATCT A^GATCT
2724 AGATCT
BsaBI 1278 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3913 GATGGCCATC
BsaHI 223 GGCGTC G[GA]^CG[CT]C
291 GGCGTC
BsgI 1835 CTGCAC GTGCAG(16/14)
270 GTGCAG
BspMI 334 ACCTGC ACCTGC(4/8)
1979 ACCTGC
BstEII 1677 GGTCACC G^GT[ACGT]ACC
3099 GGTGACC
BstPI 1677 GGTCACC G^GT[ACGT]ACC
3099 GGTGACC
EcoO65I 1677 GGTCACC G^GT[ACGT]ACC
3099 GGTGACC
EcoT14I 392 CCATGG C^C[AT][AT]GG
2342 CCATGG
Hin1I 223 GGCGTC G[GA]^CG[CT]C
291 GGCGTC
HincII 195 GTTAAC GT[CT]^[GA]AC
3126 GTCAAC
HindII 195 GTTAAC GT[CT]^[GA]AC
3126 GTCAAC
MamI 1278 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3913 GATGGCCATC
NciI 3227 CCCGG CC^[GC]GG
3944 CCGGG
NcoI 392 CCATGG C^CATGG
2342 CCATGG
Psp1406I 2193 AACGTT AA^CGTT
2675 AACGTT
StyI 392 CCATGG C^C[AT][AT]GG
2342 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BciVI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PflMI PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII