(Circular) (Six-base) MAPSORT of: pFN21AE2571.seq Check: 2214 from: 1 to: 8683 pFN21AE2571 8683 bp With 182 enzymes: SgfI * April 14, 2015 11:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2348 2348 Size: 8683 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6493 278 Size: 53 83 186 936 4957 2468 Fragments arranged by size: 4957 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6026 1937 Size: 4089 4594 Acc65I G'GTAC_C Cuts at: 4849 6004 4849 Size: 1155 7528 AclI AA'CG_TT Cuts at: 1563 4199 4681 6810 7183 1563 Size: 2636 482 2129 373 3063 Fragments arranged by size: 3063 2636 2129 482 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2191 4953 5769 6746 8130 8398 1632 Size: 559 2762 816 977 1384 268 1917 Fragments arranged by size: 2762 1917 1384 977 816 559 268 AfeI AGC'GCT Cuts at: 4839 4839 Size: 8683 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8486 AflIII A'CryG_T Cuts at: 1203 3072 7582 8559 1203 Size: 1869 4510 977 1327 Fragments arranged by size: 4510 1869 1327 977 AgeI A'CCGG_T Cuts at: 7514 7514 Size: 8683 AhdI GACnn_n'nnGTC Cuts at: 7412 7412 Size: 8683 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5519 1184 Size: 4335 4348 AlwNI CAG_nnn'CTG Cuts at: 2434 3393 6426 7998 2434 Size: 959 3033 1572 3119 Fragments arranged by size: 3119 3033 1572 959 ApaI G_GGCC'C Cuts at: 2468 2468 Size: 8683 ApaLI G'TGCA_C Cuts at: 6740 7896 6740 Size: 1156 7527 ApoI r'AATT_y Cuts at: 1493 2079 2587 2935 3559 4210 5087 5992 6218 Size: 586 508 348 624 651 877 905 226 Cuts at: 6218 6370 8479 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 905 877 651 624 586 508 348 226 152 AseI AT'TA_AT Cuts at: 160 1032 7237 160 Size: 872 6205 1606 Fragments arranged by size: 6205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8683 AvaI C'yCGr_G Cuts at: 1870 1943 2195 2303 4906 6008 1870 Size: 73 252 108 2603 1102 4545 Fragments arranged by size: 4545 2603 1102 252 108 73 BamHI G'GATC_C Cuts at: 6013 6380 6013 Size: 367 8316 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2336 4849 6004 7459 618 Size: 325 217 627 549 2513 1155 1455 1842 Fragments arranged by size: 2513 1842 1455 1155 627 549 325 217 BanII G_rGCy'C Cuts at: 1391 2021 2468 3160 3324 4907 5612 5633 6002 Size: 630 447 692 164 1583 705 21 369 Cuts at: 6002 1391 Size: 4072 Fragments arranged by size: 4072 1583 705 692 630 447 369 164 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3205 3963 4872 928 Size: 971 1306 758 909 4739 Fragments arranged by size: 4739 1306 971 909 758 BbvCI CC'TCA_GC Cuts at: 4662 4662 Size: 8683 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6874 6908 1903 Size: 34 4937 34 3678 Fragments arranged by size: 4937 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2545 4729 4784 6575 7785 2545 Size: 2184 55 1791 1210 3443 Fragments arranged by size: 3443 2184 1791 1210 55 BclI T'GATC_A Cuts at: 1548 1548 Size: 8683 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5928 7294 136 Size: 107 122 71 631 605 4256 1366 1525 Fragments arranged by size: 4256 1525 1366 631 605 122 107 71 BglII A'GATC_T Cuts at: 2715 4729 8678 2715 Size: 2014 3949 2720 Fragments arranged by size: 3949 2720 2014 BlpI GC'TnA_GC Cuts at: 2457 6100 2457 Size: 3643 5040 Bme1580I G_kGCm'C Cuts at: 1790 2468 6744 7900 1790 Size: 678 4276 1156 2573 Fragments arranged by size: 4276 2573 1156 678 BmgBI CAC'GTC Cuts at: 1318 2311 4616 1318 Size: 993 2305 5385 Fragments arranged by size: 5385 2305 993 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5655 6414 7372 Size: 416 53 83 399 24 4238 759 958 Cuts at: 7372 442 Size: 1753 Fragments arranged by size: 4238 1753 958 759 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8683 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2035 3080 3179 4945 2035 Size: 1045 99 1766 5773 Fragments arranged by size: 5773 1766 1045 99 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2355 2411 2888 3187 4317 5581 5893 7343 Size: 1116 56 477 299 1130 1264 312 1450 Cuts at: 7343 1239 Size: 2579 Fragments arranged by size: 2579 1450 1264 1130 1116 477 312 299 56 Bpu10I CC'TnA_GC Cuts at: 3318 3806 4662 3318 Size: 488 856 7339 Fragments arranged by size: 7339 856 488 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2740 3402 3656 3909 4361 6808 7673 7971 2740 Size: 662 254 253 452 2447 865 298 3452 Fragments arranged by size: 3452 2447 865 662 452 298 254 253 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 5060 5607 7346 882 Size: 617 2772 789 547 1739 2219 Fragments arranged by size: 2772 2219 1739 789 617 547 BsaAI yAC'GTr Cuts at: 493 1204 4448 6474 493 Size: 711 3244 2026 2702 Fragments arranged by size: 3244 2702 2026 711 BsaBI GATnn'nnATC Cuts at: 3287 5922 8677 3287 Size: 2635 2755 3293 Fragments arranged by size: 3293 2755 2635 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2229 2297 6490 6872 Size: 53 83 186 936 696 68 4193 382 Cuts at: 6872 7507 275 Size: 635 1451 Fragments arranged by size: 4193 1451 936 696 635 382 186 83 68 53 BsaWI w'CCGG_w Cuts at: 3222 7116 7514 7788 7935 8346 3222 Size: 3894 398 274 147 411 3559 Fragments arranged by size: 3894 3559 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2109 2139 1178 Size: 30 165 30 706 30 7722 Fragments arranged by size: 7722 706 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 4679 4715 5184 5272 5390 1186 Size: 841 2652 36 469 88 118 4479 Fragments arranged by size: 4479 2652 841 469 118 88 36 BseYI C'CCAG_C Cuts at: 3090 4065 5817 5822 5862 7886 8382 3090 Size: 975 1752 5 40 2024 496 3391 Fragments arranged by size: 3391 2024 1752 975 496 40 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2296 3825 1163 Size: 573 560 1529 6021 Fragments arranged by size: 6021 1529 573 560 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6133 6894 7922 1540 Size: 275 186 4132 761 1028 2301 Fragments arranged by size: 4132 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2021 2812 3160 3195 3418 4907 5352 5612 6744 Size: 791 348 35 223 1489 445 260 1132 Cuts at: 6744 6829 7900 2021 Size: 85 1071 2804 Fragments arranged by size: 2804 1489 1132 1071 791 445 348 260 223 85 35 BsmI GAATG_Cn' Cuts at: 4791 6194 6287 4791 Size: 1403 93 7187 Fragments arranged by size: 7187 1403 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4128 4128 Size: 8683 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2468 2812 3160 3195 3324 3418 Size: 399 231 447 344 348 35 129 94 Cuts at: 3418 4907 5352 5612 5633 6002 6744 6829 7900 Size: 1489 445 260 21 369 742 85 1071 Cuts at: 7900 1391 Size: 2174 Fragments arranged by size: 2174 1489 1071 742 447 445 399 369 348 344 260 231 129 94 85 35 21 BspEI T'CCGG_A Cuts at: 8346 8346 Size: 8683 BspHI T'CATG_A Cuts at: 2382 6572 8302 2382 Size: 4190 1730 2763 Fragments arranged by size: 4190 2763 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2348 3993 6038 844 Size: 1504 1645 2045 3489 Fragments arranged by size: 3489 2045 1645 1504 BsrBI CCG'CTC Cuts at: 1394 1823 4168 6570 1394 Size: 429 2345 2402 3507 Fragments arranged by size: 3507 2402 2345 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2634 3435 4579 7178 7352 65 Size: 1183 1386 801 1144 2599 174 1396 Fragments arranged by size: 2599 1396 1386 1183 1144 801 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7327 7514 1700 Size: 110 5517 187 2869 Fragments arranged by size: 5517 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8683 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8683 BssSI C'ACGA_G Cuts at: 3998 6743 7755 3998 Size: 2745 1012 4926 Fragments arranged by size: 4926 2745 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8683 BstBI TT'CG_AA Cuts at: 7487 7487 Size: 8683 BstEII G'GTnAC_C Cuts at: 1184 3682 5104 1184 Size: 2498 1422 4763 Fragments arranged by size: 4763 2498 1422 BstXI CCAn_nnnn'nTGG Cuts at: 2293 2746 4226 2293 Size: 453 1480 6750 Fragments arranged by size: 6750 1480 453 BstYI r'GATC_y Cuts at: 1972 2683 2715 3382 4729 6013 6380 6765 6782 Size: 711 32 667 1347 1284 367 385 17 Cuts at: 6782 8234 8320 8332 8678 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1347 1284 711 667 385 367 346 86 32 17 12 Bsu36I CC'TnA_GG Cuts at: 2345 2345 Size: 8683 BtgI C'CryG_G Cuts at: 513 1065 2397 2451 3166 4347 513 Size: 552 1332 54 715 1181 4849 Fragments arranged by size: 4849 1332 1181 715 552 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7944 Fragments arranged by size: 7944 683 56 BtsI GCAGTG_nn' Cuts at: 3785 3837 5503 6199 6993 7013 3785 Size: 52 1666 696 794 20 5455 Fragments arranged by size: 5455 1666 794 696 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8648 DraI TTT'AAA Cuts at: 4155 5987 4155 Size: 1832 6851 DraIII CAC_nnn'GTG Cuts at: 2993 2993 Size: 8683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7690 809 Size: 507 6374 1802 Fragments arranged by size: 6374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2802 3777 5803 Size: 54 1425 50 275 18 972 975 2026 Cuts at: 5803 5920 6130 7019 8648 8 Size: 117 210 889 1629 43 Fragments arranged by size: 2026 1629 1425 975 972 889 275 210 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6130 1537 Size: 275 4318 4090 Fragments arranged by size: 4318 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3165 4763 4839 4929 5397 6613 1353 Size: 1812 1598 76 90 468 1216 3423 Fragments arranged by size: 3423 1812 1598 1216 468 90 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5485 7270 7644 7790 387 Size: 1064 138 3896 1785 374 146 1280 Fragments arranged by size: 3896 1785 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2019 3158 4905 5610 2019 Size: 1139 1747 705 5092 Fragments arranged by size: 5092 1747 1139 705 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2191 2355 2411 2888 3187 4317 4953 Size: 393 559 164 56 477 299 1130 636 Cuts at: 4953 5581 5769 5893 6746 7343 8130 8398 1239 Size: 628 188 124 853 597 787 268 1524 Fragments arranged by size: 1524 1130 853 787 636 628 597 559 477 393 299 268 188 164 124 56 EcoNI CCTnn'n_nnAGG Cuts at: 2344 4040 4889 2344 Size: 1696 849 6138 Fragments arranged by size: 6138 1696 849 EcoO109I rG'GnC_Cy Cuts at: 2464 2465 2620 2464 Size: 1 155 8527 Fragments arranged by size: 8527 155 1 EcoRI G'AATT_C Cuts at: 5992 6370 5992 Size: 378 8305 EcoRV GAT'ATC Cuts at: 4492 4492 Size: 8683 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5771 5771 Size: 8683 FspI TGC'GCA Cuts at: 7189 7189 Size: 8683 HaeII r_GCGC'y Cuts at: 1678 2416 2435 4841 7830 1678 Size: 738 19 2406 2989 2531 Fragments arranged by size: 2989 2531 2406 738 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3050 3082 3389 3421 3617 Size: 32 135 32 1478 32 307 32 196 Cuts at: 3617 3649 4125 4142 4157 4174 4559 4591 4615 Size: 32 476 17 15 17 385 32 24 Cuts at: 4615 4647 5720 5752 7324 7356 7398 7430 1373 Size: 32 1073 32 1572 32 42 32 2626 Fragments arranged by size: 2626 1572 1478 1073 476 385 307 196 135 42 32 32 32 32 32 32 32 32 32 32 24 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 2202 5133 6027 6273 669 Size: 988 281 264 2931 894 246 3079 Fragments arranged by size: 3079 2931 988 894 281 264 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8683 HpaI GTT'AAC Cuts at: 2202 6273 2202 Size: 4071 4612 KpnI G_GTAC'C Cuts at: 4853 6008 4853 Size: 1155 7528 MfeI C'AATT_G Cuts at: 6282 6282 Size: 8683 MluI A'CGCG_T Cuts at: 8559 8559 Size: 8683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2085 2742 4452 7797 7981 1305 Size: 780 657 1710 3345 184 2007 Fragments arranged by size: 3345 2007 1710 780 657 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2804 3779 5805 5922 10 Size: 54 1425 343 972 975 2026 117 2771 Fragments arranged by size: 2771 2026 1425 975 972 343 117 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 2291 4224 4694 6641 7000 7159 Size: 648 1119 6 1933 470 1947 359 159 Cuts at: 7159 518 Size: 2042 Fragments arranged by size: 2042 1947 1933 1119 648 470 359 159 6 MspA1I CmG'CkG Cuts at: 1606 5009 6099 6776 7924 8169 1606 Size: 3403 1090 677 1148 245 2120 Fragments arranged by size: 3403 2120 1148 1090 677 245 NcoI C'CATG_G Cuts at: 513 1065 2397 4347 513 Size: 552 1332 1950 4849 Fragments arranged by size: 4849 1950 1332 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8683 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8683 NotI GC'GGCC_GC Cuts at: 6130 6130 Size: 8683 NspI r_CATG'y Cuts at: 1132 1229 3076 6041 7586 1132 Size: 97 1847 2965 1545 2229 Fragments arranged by size: 2965 2229 1847 1545 97 PasI CC'CwG_GG Cuts at: 1348 2031 3703 1348 Size: 683 1672 6328 Fragments arranged by size: 6328 1672 683 PciI A'CATG_T Cuts at: 3072 7582 3072 Size: 4510 4173 PflMI CCAn_nnn'nTGG Cuts at: 1108 2452 5127 5491 1108 Size: 1344 2675 364 4300 Fragments arranged by size: 4300 2675 1344 364 PfoI T'CCnGG_A Cuts at: 4143 4563 4143 Size: 420 8263 PmeI GTTT'AAAC Cuts at: 5987 5987 Size: 8683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6752 6784 8265 8297 6752 Size: 32 1481 32 7138 Fragments arranged by size: 7138 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2620 2620 Size: 8683 PshAI GACnn'nnGTC Cuts at: 1613 3680 1613 Size: 2067 6616 PsiI TTA'TAA Cuts at: 6253 6253 Size: 8683 PspOMI G'GGCC_C Cuts at: 2464 2464 Size: 8683 PspXI vC'TCGA_Gb Cuts at: 2195 2195 Size: 8683 PstI C_TGCA'G Cuts at: 830 3645 3792 4041 5649 6035 830 Size: 2815 147 249 1608 386 3478 Fragments arranged by size: 3478 2815 1608 386 249 147 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8683 SacI G_AGCT'C Cuts at: 2021 3160 4907 5612 2021 Size: 1139 1747 705 5092 Fragments arranged by size: 5092 1747 1139 705 SalI G'TCGA_C Cuts at: 1936 6025 1936 Size: 4089 4594 SapI GCTCTTCn'nnn_ Cuts at: 3165 3165 Size: 8683 SbfI CC_TGCA'GG Cuts at: 6035 6035 Size: 8683 ScaI AGT'ACT Cuts at: 6931 8643 6931 Size: 1712 6971 SfcI C'TryA_G Cuts at: 826 1045 3641 3788 4037 5645 6031 7166 7847 Size: 219 2596 147 249 1608 386 1135 681 Cuts at: 7847 8038 826 Size: 191 1471 Fragments arranged by size: 2596 1608 1471 1135 681 386 249 219 191 147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8683 SmaI CCC'GGG Cuts at: 1872 6010 1872 Size: 4138 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2195 2719 3417 3671 3924 4340 Size: 197 926 252 524 698 254 253 416 Cuts at: 4340 6787 7688 7950 820 Size: 2447 901 262 1553 Fragments arranged by size: 2447 1553 926 901 698 524 416 262 254 253 252 197 SnaBI TAC'GTA Cuts at: 493 6474 493 Size: 5981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8683 SphI G_CATG'C Cuts at: 1132 6041 1132 Size: 4909 3774 SspI AAT'ATT Cuts at: 5 52 3182 6607 5 Size: 47 3130 3425 2081 Fragments arranged by size: 3425 3130 2081 47 StuI AGG'CCT Cuts at: 1517 4028 1517 Size: 2511 6172 StyI C'CwwG_G Cuts at: 513 1065 2397 4347 6122 513 Size: 552 1332 1950 1775 3074 Fragments arranged by size: 3074 1950 1775 1332 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 4079 5015 6710 6727 6880 7065 Size: 1255 444 2055 936 1695 17 153 185 Cuts at: 7065 7510 325 Size: 445 1498 Fragments arranged by size: 2055 1695 1498 1255 936 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2536 3610 4096 Size: 274 80 33 51 1443 559 1074 486 Cuts at: 4096 6929 8641 96 Size: 2833 1712 138 Fragments arranged by size: 2833 1712 1443 1074 559 486 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5191 5223 8643 8675 5191 Size: 32 3420 32 5199 Fragments arranged by size: 5199 3420 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3146 1316 Size: 1830 6853 XbaI T'CTAG_A Cuts at: 4828 6019 4828 Size: 1191 7492 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3109 4693 5662 3109 Size: 1584 969 6130 Fragments arranged by size: 6130 1584 969 XhoI C'TCGA_G Cuts at: 1943 2195 1943 Size: 252 8431 XmaI C'CCGG_G Cuts at: 1870 6008 1870 Size: 4138 4545 XmnI GAAnn'nnTTC Cuts at: 6812 6812 Size: 8683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6491 276 Size: 53 83 186 936 4957 2468 Fragments arranged by size: 4957 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI