(Linear) (Six-base) MAPSORT of: KIEE2571.seq Check: 1020 from: 1 to: 3978 KIEE2571.orf With 182 enzymes: SgfI * April 14, 2015 09:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 343 3978 Size: 343 3635 Acc65I G'GTAC_C Cuts at: 0 2844 3978 Size: 2844 1134 AclI AA'CG_TT Cuts at: 0 2194 2676 3978 Size: 2194 482 1302 Fragments arranged by size: 2194 1302 482 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 186 2948 3764 3978 Size: 186 2762 816 214 Fragments arranged by size: 2762 816 214 186 AfeI AGC'GCT Cuts at: 0 2834 3978 Size: 2834 1144 AflIII A'CryG_T Cuts at: 0 1067 3978 Size: 1067 2911 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3514 3978 Size: 3514 464 AlwNI CAG_nnn'CTG Cuts at: 0 429 1388 3978 Size: 429 959 2590 Fragments arranged by size: 2590 959 429 ApaI G_GGCC'C Cuts at: 0 463 3978 Size: 463 3515 ApoI r'AATT_y Cuts at: 0 74 582 930 1554 2205 3082 3978 Size: 74 508 348 624 651 877 896 Fragments arranged by size: 896 877 651 624 508 348 74 AvaI C'yCGr_G Cuts at: 0 190 298 2901 3978 Size: 190 108 2603 1077 Fragments arranged by size: 2603 1077 190 108 BanI G'GyrC_C Cuts at: 0 331 2844 3978 Size: 331 2513 1134 Fragments arranged by size: 2513 1134 331 BanII G_rGCy'C Cuts at: 0 16 463 1155 1319 2902 3607 3628 3978 Size: 16 447 692 164 1583 705 21 350 Fragments arranged by size: 1583 705 692 447 350 164 21 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 1200 1958 2867 3978 Size: 1200 758 909 1111 Fragments arranged by size: 1200 1111 909 758 BbvCI CC'TCA_GC Cuts at: 0 2657 3978 Size: 2657 1321 BciVI GTATCCnnnnn_n' Cuts at: 0 540 2724 2779 3978 Size: 540 2184 55 1199 Fragments arranged by size: 2184 1199 540 55 BglI GCCn_nnn'nGGC Cuts at: 0 3923 3978 Size: 3923 55 BglII A'GATC_T Cuts at: 0 710 2724 3978 Size: 710 2014 1254 Fragments arranged by size: 2014 1254 710 BlpI GC'TnA_GC Cuts at: 0 452 3978 Size: 452 3526 Bme1580I G_kGCm'C Cuts at: 0 463 3978 Size: 463 3515 BmgBI CAC'GTC Cuts at: 0 306 2611 3978 Size: 306 2305 1367 Fragments arranged by size: 2305 1367 306 BmrI ACTGGGnnnn_n' Cuts at: 0 3650 3978 Size: 3650 328 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 30 1075 1174 2940 3978 Size: 30 1045 99 1766 1038 Fragments arranged by size: 1766 1045 1038 99 30 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 350 406 883 1182 2312 3576 3888 3978 Size: 350 56 477 299 1130 1264 312 90 Fragments arranged by size: 1264 1130 477 350 312 299 90 56 Bpu10I CC'TnA_GC Cuts at: 0 1313 1801 2657 3978 Size: 1313 488 856 1321 Fragments arranged by size: 1321 1313 856 488 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 735 1397 1651 1904 2356 3978 Size: 735 662 254 253 452 1622 Fragments arranged by size: 1622 735 662 452 254 253 BsaI GGTCTCn'nnnn_ Cuts at: 0 2266 3055 3602 3978 Size: 2266 789 547 376 Fragments arranged by size: 2266 789 547 376 BsaAI yAC'GTr Cuts at: 0 2443 3978 Size: 2443 1535 BsaBI GATnn'nnATC Cuts at: 0 1282 3917 3978 Size: 1282 2635 61 Fragments arranged by size: 2635 1282 61 BsaHI Gr'CG_yC Cuts at: 0 224 292 3978 Size: 224 68 3686 Fragments arranged by size: 3686 224 68 BsaWI w'CCGG_w Cuts at: 0 1217 3978 Size: 1217 2761 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 104 134 3978 Size: 104 30 3844 Fragments arranged by size: 3844 104 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 22 2674 2710 3179 3267 3385 3978 Size: 22 2652 36 469 88 118 593 Fragments arranged by size: 2652 593 469 118 88 36 22 BseYI C'CCAG_C Cuts at: 0 1085 2060 3812 3817 3857 3978 Size: 1085 975 1752 5 40 121 Fragments arranged by size: 1752 1085 975 121 40 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 291 1820 3978 Size: 291 1529 2158 Fragments arranged by size: 2158 1529 291 BsiHKAI G_wGCw'C Cuts at: 0 16 807 1155 1190 1413 2902 3347 3607 Size: 16 791 348 35 223 1489 445 260 Cuts at: 3607 3978 Size: 371 Fragments arranged by size: 1489 791 445 371 348 260 223 35 16 BsmI GAATG_Cn' Cuts at: 0 2786 3978 Size: 2786 1192 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2123 3978 Size: 2123 1855 Bsp1286I G_dGCh'C Cuts at: 0 16 463 807 1155 1190 1319 1413 2902 Size: 16 447 344 348 35 129 94 1489 Cuts at: 2902 3347 3607 3628 3978 Size: 445 260 21 350 Fragments arranged by size: 1489 447 445 350 348 344 260 129 94 35 21 16 BspHI T'CATG_A Cuts at: 0 377 3978 Size: 377 3601 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 343 1988 3978 Size: 343 1645 1990 Fragments arranged by size: 1990 1645 343 BsrBI CCG'CTC Cuts at: 0 2163 3978 Size: 2163 1815 BsrDI GCAATG_nn' Cuts at: 0 629 1430 2574 3978 Size: 629 801 1144 1404 Fragments arranged by size: 1404 1144 801 629 BssSI C'ACGA_G Cuts at: 0 1993 3978 Size: 1993 1985 BstEII G'GTnAC_C Cuts at: 0 1677 3099 3978 Size: 1677 1422 879 Fragments arranged by size: 1677 1422 879 BstXI CCAn_nnnn'nTGG Cuts at: 0 288 741 2221 3978 Size: 288 453 1480 1757 Fragments arranged by size: 1757 1480 453 288 BstYI r'GATC_y Cuts at: 0 678 710 1377 2724 3978 Size: 678 32 667 1347 1254 Fragments arranged by size: 1347 1254 678 667 32 Bsu36I CC'TnA_GG Cuts at: 0 340 3978 Size: 340 3638 BtgI C'CryG_G Cuts at: 0 392 446 1161 2342 3978 Size: 392 54 715 1181 1636 Fragments arranged by size: 1636 1181 715 392 54 BtsI GCAGTG_nn' Cuts at: 0 1780 1832 3498 3978 Size: 1780 52 1666 480 Fragments arranged by size: 1780 1666 480 52 DraI TTT'AAA Cuts at: 0 2150 3978 Size: 2150 1828 DraIII CAC_nnn'GTG Cuts at: 0 988 3978 Size: 988 2990 EaeI y'GGCC_r Cuts at: 0 797 1772 3798 3915 3978 Size: 797 975 2026 117 63 Fragments arranged by size: 2026 975 797 117 63 EarI CTCTTCn'nnn_ Cuts at: 0 1160 2758 2834 2924 3392 3978 Size: 1160 1598 76 90 468 586 Fragments arranged by size: 1598 1160 586 468 90 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3480 3978 Size: 3480 498 EcoICRI GAG'CTC Cuts at: 0 14 1153 2900 3605 3978 Size: 14 1139 1747 705 373 Fragments arranged by size: 1747 1139 705 373 14 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 186 350 406 883 1182 2312 2948 3576 Size: 186 164 56 477 299 1130 636 628 Cuts at: 3576 3764 3888 3978 Size: 188 124 90 Fragments arranged by size: 1130 636 628 477 299 188 186 164 124 90 56 EcoNI CCTnn'n_nnAGG Cuts at: 0 339 2035 2884 3978 Size: 339 1696 849 1094 Fragments arranged by size: 1696 1094 849 339 EcoO109I rG'GnC_Cy Cuts at: 0 459 460 615 3978 Size: 459 1 155 3363 Fragments arranged by size: 3363 459 155 1 EcoRV GAT'ATC Cuts at: 0 2487 3978 Size: 2487 1491 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 3766 3978 Size: 3766 212 HaeII r_GCGC'y Cuts at: 0 411 430 2836 3978 Size: 411 19 2406 1142 Fragments arranged by size: 2406 1142 411 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1045 1077 1384 1416 1612 1644 2120 2137 Size: 1045 32 307 32 196 32 476 17 Cuts at: 2137 2152 2169 2554 2586 2610 2642 3715 3747 Size: 15 17 385 32 24 32 1073 32 Cuts at: 3747 3978 Size: 231 Fragments arranged by size: 1073 1045 476 385 307 231 196 32 32 32 32 32 32 24 17 17 15 HincII GTy'rAC Cuts at: 0 197 3128 3978 Size: 197 2931 850 Fragments arranged by size: 2931 850 197 HpaI GTT'AAC Cuts at: 0 197 3978 Size: 197 3781 KpnI G_GTAC'C Cuts at: 0 2848 3978 Size: 2848 1130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 80 737 2447 3978 Size: 80 657 1710 1531 Fragments arranged by size: 1710 1531 657 80 MscI TGG'CCA Cuts at: 0 799 1774 3800 3917 3978 Size: 799 975 2026 117 61 Fragments arranged by size: 2026 975 799 117 61 MslI CAynn'nnrTG Cuts at: 0 280 286 2219 2689 3978 Size: 280 6 1933 470 1289 Fragments arranged by size: 1933 1289 470 280 6 MspA1I CmG'CkG Cuts at: 0 3004 3978 Size: 3004 974 NcoI C'CATG_G Cuts at: 0 392 2342 3978 Size: 392 1950 1636 Fragments arranged by size: 1950 1636 392 NspI r_CATG'y Cuts at: 0 1071 3978 Size: 1071 2907 PasI CC'CwG_GG Cuts at: 0 26 1698 3978 Size: 26 1672 2280 Fragments arranged by size: 2280 1672 26 PciI A'CATG_T Cuts at: 0 1067 3978 Size: 1067 2911 PflMI CCAn_nnn'nTGG Cuts at: 0 447 3122 3486 3978 Size: 447 2675 364 492 Fragments arranged by size: 2675 492 447 364 PfoI T'CCnGG_A Cuts at: 0 2138 2558 3978 Size: 2138 420 1420 Fragments arranged by size: 2138 1420 420 PpuMI rG'GwC_Cy Cuts at: 0 615 3978 Size: 615 3363 PshAI GACnn'nnGTC Cuts at: 0 1675 3978 Size: 1675 2303 PspOMI G'GGCC_C Cuts at: 0 459 3978 Size: 459 3519 PspXI vC'TCGA_Gb Cuts at: 0 190 3978 Size: 190 3788 PstI C_TGCA'G Cuts at: 0 1640 1787 2036 3644 3978 Size: 1640 147 249 1608 334 Fragments arranged by size: 1640 1608 334 249 147 SacI G_AGCT'C Cuts at: 0 16 1155 2902 3607 3978 Size: 16 1139 1747 705 371 Fragments arranged by size: 1747 1139 705 371 16 SapI GCTCTTCn'nnn_ Cuts at: 0 1160 3978 Size: 1160 2818 SfcI C'TryA_G Cuts at: 0 1636 1783 2032 3640 3978 Size: 1636 147 249 1608 338 Fragments arranged by size: 1636 1608 338 249 147 SmlI C'TyrA_G Cuts at: 0 190 714 1412 1666 1919 2335 3978 Size: 190 524 698 254 253 416 1643 Fragments arranged by size: 1643 698 524 416 254 253 190 SspI AAT'ATT Cuts at: 0 1177 3978 Size: 1177 2801 StuI AGG'CCT Cuts at: 0 2023 3978 Size: 2023 1955 StyI C'CwwG_G Cuts at: 0 392 2342 3978 Size: 392 1950 1636 Fragments arranged by size: 1950 1636 392 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 19 2074 3010 3978 Size: 19 2055 936 968 Fragments arranged by size: 2055 968 936 19 TatI w'GTAC_w Cuts at: 0 531 1605 2091 3978 Size: 531 1074 486 1887 Fragments arranged by size: 1887 1074 531 486 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3186 3218 3978 Size: 3186 32 760 Fragments arranged by size: 3186 760 32 Tth111I GACn'n_nGTC Cuts at: 0 1141 3978 Size: 1141 2837 XbaI T'CTAG_A Cuts at: 0 2823 3978 Size: 2823 1155 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1104 2688 3657 3978 Size: 1104 1584 969 321 Fragments arranged by size: 1584 1104 969 321 XhoI C'TCGA_G Cuts at: 0 190 3978 Size: 190 3788 Enzymes that do cut: AarI Acc65I AclI AcuI AfeI AflIII AlfI AlwNI ApaI ApoI AvaI BanI BanII BbsI BbvCI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HpaI KpnI MmeI MscI MslI MspA1I NcoI NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PspXI PstI SacI SapI SfcI SmlI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI Enzymes that do not cut: AatII AccI AflII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BclI BfrBI BmtI BsiEI BsiWI BspEI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI ClaI CspCI DrdI EagI EcoRI FseI FspI FspAI HindIII KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PsiI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI XmaI XmnI ZraI