################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AviII AvrII BbeI BbrPI BcgI BciVI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DsaI EagI EclXI Eco47III Eco52I Eco81I EcoRV EcoT14I FauI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SspBI SspI StyI SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 691 CGCG CG^CG AflII 982 CTTAAG C^TTAAG AhdI 1079 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 735 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1079 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 757 GAGCTC G[AT]GC[AT]^C AvaI 428 CCCGGG C^[CT]CG[GA]G BamHI 1460 GGATCC G^GATCC BbvCI 591 CCTCAGC CCTCAGC(-5/-2) BclI 1443 TGATCA T^GATCA BfrI 982 CTTAAG C^TTAAG BsiHKAI 757 GAGCTC G[AT]GC[AT]^C BsmAI 1508 GAGAC GTCTC(1/5) BsoBI 428 CCCGGG C^[CT]CG[GA]G BspHI 1398 TCATGA T^CATGA BsrDI 1186 CATTGC GCAATG(2/0) BstEII 703 GGTTACC G^GT[ACGT]ACC BstPI 703 GGTTACC G^GT[ACGT]ACC BstUI 691 CGCG CG^CG BstXI 1380 CCAGTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 498 GCGC GCG^C DrdI 674 GACTAAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1079 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 703 GGTTACC G^GT[ACGT]ACC EcoRI 847 GAATTC G^AATTC EcoT22I 610 ATGCAT ATGCA^T FbaI 1443 TGATCA T^GATCA FseI 381 GGCCGGCC GGCCGG^CC HaeII 497 GGCGCT [GA]GCGC^[CT] HhaI 498 GCGC GCG^C HinP1I 498 GCGC G^CGC HindIII 226 AAGCTT A^AGCTT MslI 1435 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 691 CGCG CG^CG NaeI 382 GCCGGC GCC^GGC NgoMIV 382 GCCGGC G^CCGGC NsiI 610 ATGCAT ATGCA^T PflMI 796 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1079 GACTC GAGTC(4/5) PstI 552 CTGCAG CTGCA^G RcaI 1398 TCATGA T^CATGA SacI 757 GAGCTC GAGCT^C SmaI 428 CCCGGG CCC^GGG SpeI 346 ACTAGT A^CTAGT Van91I 796 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 120 TCTAGA T^CTAGA XmaCI 428 CCCGGG C^CCGGG XmaI 428 CCCGGG C^CCGGG XmnI 735 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 93 GAATTT [GA]^AATT[CT] 847 GAATTC AfaI 818 GTAC GT^AC 979 GTAC ApoI 93 GAATTT [GA]^AATT[CT] 847 GAATTC AspI 355 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 674 GACTAAGTC BalI 188 TGGCCA TGG^CCA 917 TGGCCA BanI 835 GGCACC G^G[CT][GA]CC 1014 GGCACC BanII 579 GAGCCC G[GA]GC[CT]^C 757 GAGCTC BbsI 361 GTCTTC GAAGAC(2/6) 1087 GTCTTC BpuAI 361 GTCTTC GAAGAC(2/6) 1087 GTCTTC BsrFI 382 GCCGGC [GA]^CCGG[CT] 707 ACCGGC BstYI 1048 AGATCC [GA]^GATC[CT] 1460 GGATCC Cfr10I 382 GCCGGC [GA]^CCGG[CT] 707 ACCGGC EcoNI 141 CCTTTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1032 CCTCTGTCAGG HgaI 605 GACGC GACGC(5/10) 692 GCGTC MflI 1048 AGATCC [GA]^GATC[CT] 1460 GGATCC MluNI 188 TGGCCA TGG^CCA 917 TGGCCA MscI 188 TGGCCA TGG^CCA 917 TGGCCA MseI 170 TTAA T^TAA 983 TTAA NspI 370 GCATGC [GA]CATG^[CT] 608 GCATGC PflFI 355 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 674 GACTAAGTC RsaI 818 GTAC GT^AC 979 GTAC SapI 735 GAAGAGC GCTCTTC(1/4) 1251 GAAGAGC SfcI 510 CTATAG C^T[GA][CT]AG 552 CTGCAG SphI 370 GCATGC GCATG^C 608 GCATGC TaqI 23 TCGA T^CGA 312 TCGA Tru9I 170 TTAA T^TAA 983 TTAA Tsp509I 94 AATT ^AATT 848 AATT Tth111I 355 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 674 GACTAAGTC TthHB8I 23 TCGA T^CGA 312 TCGA XhoII 1048 AGATCC [GA]^GATC[CT] 1460 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PvuII Sau3AI Sau96I ScrFI SfaNI SmlI StuI TfiI TseI Tsp45I TspRI