Restriction map for
pFN21AE2231
(Circular) (Six-base) MAPSORT of: pFN21AE2231.seq Check: 7694 from: 1 to: 6253
pFN21AE2231 6253 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4063 278
Size: 53 83 186 936 2527 2468
Fragments arranged by size:
2527 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3596 1937
Size: 1659 4594
Acc65I G'GTAC_C
Cuts at: 3574 3574
Size: 6253
AclI AA'CG_TT
Cuts at: 1563 4380 4753 1563
Size: 2817 373 3063
Fragments arranged by size:
3063 2817 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 4316 5700 5968 1632
Size: 407 2277 1384 268 1917
Fragments arranged by size:
2277 1917 1384 407 268
AflII C'TTAA_G
Cuts at: 820 1017 2987 820
Size: 197 1970 4086
Fragments arranged by size:
4086 1970 197
AflIII A'CryG_T
Cuts at: 1203 5152 6129 1203
Size: 3949 977 1327
Fragments arranged by size:
3949 1327 977
AgeI A'CCGG_T
Cuts at: 5084 5084
Size: 6253
AhdI GACnn_n'nnGTC
Cuts at: 3089 4982 3089
Size: 1893 4360
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6253
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2941 2973 2941
Size: 32 6221
AlwNI CAG_nnn'CTG
Cuts at: 3996 5568 3996
Size: 1572 4681
ApaLI G'TGCA_C
Cuts at: 4310 5466 4310
Size: 1156 5097
ApoI r'AATT_y
Cuts at: 1493 2098 2852 3562 3788 3940 6049 1493
Size: 605 754 710 226 152 2109 1697
Fragments arranged by size:
2109 1697 754 710 605 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4807 160
Size: 872 3775 1606
Fragments arranged by size:
3775 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
AvaI C'yCGr_G
Cuts at: 1870 1943 2433 3578 1870
Size: 73 490 1145 4545
Fragments arranged by size:
4545 1145 490 73
BamHI G'GATC_C
Cuts at: 3465 3583 3950 3465
Size: 118 367 5768
Fragments arranged by size:
5768 367 118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2840 3019 3574 5029 618
Size: 325 217 627 1053 179 555 1455 1842
Fragments arranged by size:
1842 1455 1053 627 555 325 217 179
BanII G_rGCy'C
Cuts at: 1391 2588 2766 3572 1391
Size: 1197 178 806 4072
Fragments arranged by size:
4072 1197 806 178
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2359 3085 928
Size: 971 460 726 4096
Fragments arranged by size:
4096 971 726 460
BbvCI CC'TCA_GC
Cuts at: 2597 2597
Size: 6253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4444 4478 1903
Size: 34 2507 34 3678
Fragments arranged by size:
3678 2507 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4145 5355 4145
Size: 1210 5043
BclI T'GATC_A
Cuts at: 1548 3448 1548
Size: 1900 4353
BfrBI ATG'CAT
Cuts at: 2617 2617
Size: 6253
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4864 136
Size: 107 122 71 631 605 3192 1525
Fragments arranged by size:
3192 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6248 6248
Size: 6253
BlpI GC'TnA_GC
Cuts at: 3670 3670
Size: 6253
Bme1580I G_kGCm'C
Cuts at: 1790 2399 4314 5470 1790
Size: 609 1915 1156 2573
Fragments arranged by size:
2573 1915 1156 609
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6253
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2325 3984 4942
Size: 416 53 83 399 24 908 1659 958
Cuts at: 4942 442
Size: 1753
Fragments arranged by size:
1753 1659 958 908 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2065 3127 3340 4913 1239
Size: 826 1062 213 1573 2579
Fragments arranged by size:
2579 1573 1062 826 213
Bpu10I CC'TnA_GC
Cuts at: 2597 2597
Size: 6253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2255 3064 4378 5243 5541 2255
Size: 809 1314 865 298 2967
Fragments arranged by size:
2967 1314 865 809 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4916 882
Size: 617 3417 2219
Fragments arranged by size:
3417 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4044 493
Size: 711 2840 2702
Fragments arranged by size:
2840 2702 711
BsaBI GATnn'nnATC
Cuts at: 6247 6247
Size: 6253
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4060 4442 5077 275
Size: 53 83 186 936 2527 382 635 1451
Fragments arranged by size:
2527 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4686 5084 5358 5505 5916 4686
Size: 398 274 147 411 5023
Fragments arranged by size:
5023 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6028
Fragments arranged by size:
6028 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6253
BseYI C'CCAG_C
Cuts at: 2410 2414 2456 3422 5456 5952 2410
Size: 4 42 966 2034 496 2711
Fragments arranged by size:
2711 2034 966 496 42 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5680
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3703 4464 5492 1540
Size: 275 186 1702 761 1028 2301
Fragments arranged by size:
2301 1702 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2766 4314 4399 5470 2766
Size: 1548 85 1071 3549
Fragments arranged by size:
3549 1548 1071 85
BsmI GAATG_Cn'
Cuts at: 3764 3857 3764
Size: 93 6160
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2399 2588 2766 3572 4314 4399 5470
Size: 399 609 189 178 806 742 85 1071
Cuts at: 5470 1391
Size: 2174
Fragments arranged by size:
2174 1071 806 742 609 399 189 178
85
BspEI T'CCGG_A
Cuts at: 5916 5916
Size: 6253
BspHI T'CATG_A
Cuts at: 3403 4142 5872 3403
Size: 739 1730 3784
Fragments arranged by size:
3784 1730 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3608 844
Size: 2764 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4140 1394
Size: 429 2317 3507
Fragments arranged by size:
3507 2317 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3190 4748 4922 65
Size: 1183 1942 1558 174 1396
Fragments arranged by size:
1942 1558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2387 2712 4897 5084 1700
Size: 110 577 325 2185 187 2869
Fragments arranged by size:
2869 2185 577 325 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6253
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6253
BssSI C'ACGA_G
Cuts at: 4313 5325 4313
Size: 1012 5241
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6253
BstBI TT'CG_AA
Cuts at: 5057 5057
Size: 6253
BstEII G'GTnAC_C
Cuts at: 1184 2708 1184
Size: 1524 4729
BstXI CCAn_nnnn'nTGG
Cuts at: 3392 3392
Size: 6253
BstYI r'GATC_y
Cuts at: 1972 3053 3465 3583 3950 4335 4352 5804 5890
Size: 1081 412 118 367 385 17 1452 86
Cuts at: 5890 5902 6248 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1081 412 385 367 346 118
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5701
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2851 487
Size: 683 56 1625 3889
Fragments arranged by size:
3889 1625 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 3537 3769 4563 4583 2100
Size: 1437 232 794 20 3770
Fragments arranged by size:
3770 1437 794 232 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6253
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6218
DraI TTT'AAA
Cuts at: 3557 3557
Size: 6253
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2685 5260 809
Size: 507 1369 2575 1802
Fragments arranged by size:
2575 1802 1369 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2389 2922
Size: 54 1425 50 275 18 363 196 533
Cuts at: 2922 3700 4589 6218 8
Size: 778 889 1629 43
Fragments arranged by size:
1629 1425 889 778 533 363 275 196
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3700 1537
Size: 275 1888 4090
Fragments arranged by size:
4090 1888 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2106 2238 2735 3251 3478 4183 1353
Size: 753 132 497 516 227 705 3423
Fragments arranged by size:
3423 753 705 516 497 227 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4840 5214 5360 387
Size: 1064 138 3251 374 146 1280
Fragments arranged by size:
3251 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2764 2764
Size: 6253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2065 3127 3340 4316 4913 5700
Size: 393 407 26 1062 213 976 597 787
Cuts at: 5700 5968 1239
Size: 268 1524
Fragments arranged by size:
1524 1062 976 787 597 407 393 268
213 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 3041 2150
Size: 891 5362
EcoO109I rG'GnC_Cy
Cuts at: 2143 2430 2631 2928 2957 3012 3304 3340 2143
Size: 287 201 297 29 55 292 36 5056
Fragments arranged by size:
5056 297 292 287 201 55 36 29
EcoRI G'AATT_C
Cuts at: 2852 3562 3940 2852
Size: 710 378 5165
Fragments arranged by size:
5165 710 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3323 3323
Size: 6253
FseI GG_CCGG'CC
Cuts at: 2391 2391
Size: 6253
FspI TGC'GCA
Cuts at: 4759 4759
Size: 6253
HaeII r_GCGC'y
Cuts at: 1678 2506 5400 1678
Size: 828 2894 2531
Fragments arranged by size:
2894 2531 828
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 2250 2282 3038
Size: 32 135 32 575 32 71 32 756
Cuts at: 3038 3070 3075 3107 4894 4926 4968 5000 1373
Size: 32 5 32 1787 32 42 32 2626
Fragments arranged by size:
2626 1787 756 575 135 71 42 32
32 32 32 32 32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3597 3843 669
Size: 988 281 1659 246 3079
Fragments arranged by size:
3079 1659 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2231 748
Size: 1483 4770
HpaI GTT'AAC
Cuts at: 3843 3843
Size: 6253
KpnI G_GTAC'C
Cuts at: 3578 3578
Size: 6253
MfeI C'AATT_G
Cuts at: 3852 3852
Size: 6253
MluI A'CGCG_T
Cuts at: 6129 6129
Size: 6253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 3081 5367 5551 1305
Size: 840 936 2286 184 2007
Fragments arranged by size:
2286 2007 936 840 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2195 2924 10
Size: 54 1425 343 363 729 3339
Fragments arranged by size:
3339 1425 729 363 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3444 4211 4570 4729 518
Size: 648 2278 767 359 159 2042
Fragments arranged by size:
2278 2042 767 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2414 2556 3669 4346 5494 5739 1606
Size: 411 397 142 1113 677 1148 245 2120
Fragments arranged by size:
2120 1148 1113 677 411 397 245 142
NaeI GCC'GGC
Cuts at: 2389 2389
Size: 6253
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5701
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6253
NgoMIV G'CCGG_C
Cuts at: 2387 2387
Size: 6253
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6253
NotI GC'GGCC_GC
Cuts at: 3700 3700
Size: 6253
NsiI A_TGCA'T
Cuts at: 2619 2619
Size: 6253
NspI r_CATG'y
Cuts at: 1132 1229 2379 2617 3611 5156 1132
Size: 97 1150 238 994 1545 2229
Fragments arranged by size:
2229 1545 1150 994 238 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6253
PciI A'CATG_T
Cuts at: 5152 5152
Size: 6253
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2807 1108
Size: 1699 4554
PfoI T'CCnGG_A
Cuts at: 2748 2748
Size: 6253
PmeI GTTT'AAAC
Cuts at: 3557 3557
Size: 6253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4322 4354 5835 5867 4322
Size: 32 1481 32 4708
Fragments arranged by size:
4708 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2143 2430 2928 2957 3012 3340 2143
Size: 287 498 29 55 328 5056
Fragments arranged by size:
5056 498 328 287 55 29
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6253
PsiI TTA'TAA
Cuts at: 3823 3823
Size: 6253
PstI C_TGCA'G
Cuts at: 830 2561 3605 830
Size: 1731 1044 3478
Fragments arranged by size:
3478 1731 1044
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6253
PvuII CAG'CTG
Cuts at: 2017 2414 2556 2017
Size: 397 142 5714
Fragments arranged by size:
5714 397 142
SacI G_AGCT'C
Cuts at: 2766 2766
Size: 6253
SalI G'TCGA_C
Cuts at: 1936 3595 1936
Size: 1659 4594
SapI GCTCTTCn'nnn_
Cuts at: 2735 3251 2735
Size: 516 5737
SbfI CC_TGCA'GG
Cuts at: 3605 3605
Size: 6253
ScaI AGT'ACT
Cuts at: 4501 6213 4501
Size: 1712 4541
SfcI C'TryA_G
Cuts at: 826 1045 2515 2557 3601 4736 5417 5608 826
Size: 219 1470 42 1044 1135 681 191 1471
Fragments arranged by size:
1471 1470 1135 1044 681 219 191 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6253
SmaI CCC'GGG
Cuts at: 1872 2435 3580 1872
Size: 563 1145 4545
Fragments arranged by size:
4545 1145 563
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 2987 3079 4357 5258 5520
Size: 197 926 291 753 92 1278 901 262
Cuts at: 5520 820
Size: 1553
Fragments arranged by size:
1553 1278 926 901 753 291 262 197
92
SnaBI TAC'GTA
Cuts at: 493 4044 493
Size: 3551 2702
SpeI A'CTAG_T
Cuts at: 152 2351 152
Size: 2199 4054
SphI G_CATG'C
Cuts at: 1132 2379 2617 3611 1132
Size: 1247 238 994 3774
Fragments arranged by size:
3774 1247 994 238
SspI AAT'ATT
Cuts at: 5 52 4177 5
Size: 47 4125 2081
Fragments arranged by size:
4125 2081 47
StuI AGG'CCT
Cuts at: 1517 2289 3489 3503 1517
Size: 772 1200 14 4267
Fragments arranged by size:
4267 1200 772 14
StyI C'CwwG_G
Cuts at: 513 1065 3692 513
Size: 552 2627 3074
Fragments arranged by size:
3074 2627 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2120 2858 2878 3037 4280 4297 4450
Size: 1255 540 738 20 159 1243 17 153
Cuts at: 4450 4635 5080 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1243 738 540 445 185 159
153 20 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2822 2983 4499
Size: 274 80 33 51 1443 845 161 1516
Cuts at: 4499 6211 96
Size: 1712 138
Fragments arranged by size:
1712 1516 1443 845 274 161 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6213 6245 6213
Size: 32 6221
Tth111I GACn'n_nGTC
Cuts at: 1316 2363 2682 1316
Size: 1047 319 4887
Fragments arranged by size:
4887 1047 319
XbaI T'CTAG_A
Cuts at: 2125 3589 2125
Size: 1464 4789
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6253
XmaI C'CCGG_G
Cuts at: 1870 2433 3578 1870
Size: 563 1145 4545
Fragments arranged by size:
4545 1145 563
XmnI GAAnn'nnTTC
Cuts at: 2744 4382 2744
Size: 1638 4615
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4061 276
Size: 53 83 186 936 2527 2468
Fragments arranged by size:
2527 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FspAI KasI
NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI