(Linear) (Six-base) MAPSORT of: KIEE2231.seq Check: 7295 from: 1 to: 1548 KIEE2231.orf With 182 enzymes: SgfI * July 3, 2012 17:17 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 1548 Size: 34 1514 AflII C'TTAA_G Cuts at: 0 982 1548 Size: 982 566 AhdI GACnn_n'nnGTC Cuts at: 0 1084 1548 Size: 1084 464 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 936 968 1548 Size: 936 32 580 Fragments arranged by size: 936 580 32 ApoI r'AATT_y Cuts at: 0 93 847 1548 Size: 93 754 701 Fragments arranged by size: 754 701 93 AvaI C'yCGr_G Cuts at: 0 428 1548 Size: 428 1120 BamHI G'GATC_C Cuts at: 0 1460 1548 Size: 1460 88 BanI G'GyrC_C Cuts at: 0 835 1014 1548 Size: 835 179 534 Fragments arranged by size: 835 534 179 BanII G_rGCy'C Cuts at: 0 583 761 1548 Size: 583 178 787 Fragments arranged by size: 787 583 178 BbsI GAAGACnn'nnnn_ Cuts at: 0 354 1080 1548 Size: 354 726 468 Fragments arranged by size: 726 468 354 BbvCI CC'TCA_GC Cuts at: 0 592 1548 Size: 592 956 BclI T'GATC_A Cuts at: 0 1443 1548 Size: 1443 105 BfrBI ATG'CAT Cuts at: 0 612 1548 Size: 612 936 Bme1580I G_kGCm'C Cuts at: 0 394 1548 Size: 394 1154 BmrI ACTGGGnnnn_n' Cuts at: 0 320 1548 Size: 320 1228 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 1122 1335 1548 Size: 60 1062 213 213 Fragments arranged by size: 1062 213 213 60 Bpu10I CC'TnA_GC Cuts at: 0 592 1548 Size: 592 956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 1059 1548 Size: 250 809 489 Fragments arranged by size: 809 489 250 BseYI C'CCAG_C Cuts at: 0 405 409 451 1417 1548 Size: 405 4 42 966 131 Fragments arranged by size: 966 405 131 42 4 BsiHKAI G_wGCw'C Cuts at: 0 761 1548 Size: 761 787 Bsp1286I G_dGCh'C Cuts at: 0 394 583 761 1548 Size: 394 189 178 787 Fragments arranged by size: 787 394 189 178 BspHI T'CATG_A Cuts at: 0 1398 1548 Size: 1398 150 BsrDI GCAATG_nn' Cuts at: 0 1185 1548 Size: 1185 363 BsrFI r'CCGG_y Cuts at: 0 382 707 1548 Size: 382 325 841 Fragments arranged by size: 841 382 325 BstEII G'GTnAC_C Cuts at: 0 703 1548 Size: 703 845 BstXI CCAn_nnnn'nTGG Cuts at: 0 1387 1548 Size: 1387 161 BstYI r'GATC_y Cuts at: 0 1048 1460 1548 Size: 1048 412 88 Fragments arranged by size: 1048 412 88 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 846 1548 Size: 846 702 BtsI GCAGTG_nn' Cuts at: 0 95 1532 1548 Size: 95 1437 16 Fragments arranged by size: 1437 95 16 DrdI GACnn_nn'nnGTC Cuts at: 0 680 1548 Size: 680 868 EaeI y'GGCC_r Cuts at: 0 188 384 917 1548 Size: 188 196 533 631 Fragments arranged by size: 631 533 196 188 EarI CTCTTCn'nnn_ Cuts at: 0 101 233 730 1246 1473 1548 Size: 101 132 497 516 227 75 Fragments arranged by size: 516 497 227 132 101 75 EcoICRI GAG'CTC Cuts at: 0 759 1548 Size: 759 789 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 60 1122 1335 1548 Size: 34 26 1062 213 213 Fragments arranged by size: 1062 213 213 34 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 145 1036 1548 Size: 145 891 512 Fragments arranged by size: 891 512 145 EcoO109I rG'GnC_Cy Cuts at: 0 138 425 626 923 952 1007 1299 1335 Size: 138 287 201 297 29 55 292 36 Cuts at: 1335 1548 Size: 213 Fragments arranged by size: 297 292 287 213 201 138 55 36 29 EcoRI G'AATT_C Cuts at: 0 847 1548 Size: 847 701 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1318 1548 Size: 1318 230 FseI GG_CCGG'CC Cuts at: 0 386 1548 Size: 386 1162 HaeII r_GCGC'y Cuts at: 0 501 1548 Size: 501 1047 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 142 174 245 277 1033 1065 1070 1102 Size: 142 32 71 32 756 32 5 32 Cuts at: 1102 1548 Size: 446 Fragments arranged by size: 756 446 142 71 32 32 32 32 5 HindIII A'AGCT_T Cuts at: 0 226 1548 Size: 226 1322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 140 1076 1548 Size: 140 936 472 Fragments arranged by size: 936 472 140 MscI TGG'CCA Cuts at: 0 190 919 1548 Size: 190 729 629 Fragments arranged by size: 729 629 190 MslI CAynn'nnrTG Cuts at: 0 1439 1548 Size: 1439 109 MspA1I CmG'CkG Cuts at: 0 12 409 551 1548 Size: 12 397 142 997 Fragments arranged by size: 997 397 142 12 NaeI GCC'GGC Cuts at: 0 384 1548 Size: 384 1164 NgoMIV G'CCGG_C Cuts at: 0 382 1548 Size: 382 1166 NsiI A_TGCA'T Cuts at: 0 614 1548 Size: 614 934 NspI r_CATG'y Cuts at: 0 374 612 1548 Size: 374 238 936 Fragments arranged by size: 936 374 238 PflMI CCAn_nnn'nTGG Cuts at: 0 802 1548 Size: 802 746 PfoI T'CCnGG_A Cuts at: 0 743 1548 Size: 743 805 PpuMI rG'GwC_Cy Cuts at: 0 138 425 923 952 1007 1335 1548 Size: 138 287 498 29 55 328 213 Fragments arranged by size: 498 328 287 213 138 55 29 PstI C_TGCA'G Cuts at: 0 556 1548 Size: 556 992 PvuII CAG'CTG Cuts at: 0 12 409 551 1548 Size: 12 397 142 997 Fragments arranged by size: 997 397 142 12 SacI G_AGCT'C Cuts at: 0 761 1548 Size: 761 787 SapI GCTCTTCn'nnn_ Cuts at: 0 730 1246 1548 Size: 730 516 302 Fragments arranged by size: 730 516 302 SfcI C'TryA_G Cuts at: 0 510 552 1548 Size: 510 42 996 Fragments arranged by size: 996 510 42 SmaI CCC'GGG Cuts at: 0 430 1548 Size: 430 1118 SmlI C'TyrA_G Cuts at: 0 229 982 1074 1548 Size: 229 753 92 474 Fragments arranged by size: 753 474 229 92 SpeI A'CTAG_T Cuts at: 0 346 1548 Size: 346 1202 SphI G_CATG'C Cuts at: 0 374 612 1548 Size: 374 238 936 Fragments arranged by size: 936 374 238 StuI AGG'CCT Cuts at: 0 284 1484 1498 1548 Size: 284 1200 14 50 Fragments arranged by size: 1200 284 50 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 115 853 873 1032 1548 Size: 115 738 20 159 516 Fragments arranged by size: 738 516 159 115 20 TatI w'GTAC_w Cuts at: 0 817 978 1548 Size: 817 161 570 Fragments arranged by size: 817 570 161 Tth111I GACn'n_nGTC Cuts at: 0 358 677 1548 Size: 358 319 871 Fragments arranged by size: 871 358 319 XbaI T'CTAG_A Cuts at: 0 120 1548 Size: 120 1428 XmaI C'CCGG_G Cuts at: 0 428 1548 Size: 428 1120 XmnI GAAnn'nnTTC Cuts at: 0 739 1548 Size: 739 809 Enzymes that do cut: AcuI AflII AhdI AloI ApoI AvaI BamHI BanI BanII BbsI BbvCI BclI BfrBI Bme1580I BmrI BpmI Bpu10I BpuEI BseYI BsiHKAI Bsp1286I BspHI BsrDI BsrFI BstEII BstXI BstYI BtgZI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NaeI NgoMIV NsiI NspI PflMI PfoI PpuMI PstI PvuII SacI SapI SfcI SmaI SmlI SpeI SphI StuI TaqII TatI Tth111I XbaI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BglI BglII BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII EagI EciI EcoRV FspI FspAI HincII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SrfI SspI StyI SwaI TstI XcmI XhoI ZraI