Restriction map for
pFN21AE3824
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccIII AgeI ApaI
AscI Asp718I AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BglI
BlnI BsaAI BseAI BsiWI BspDI
BspEI BsrBI BssHII Bst1107I BstAPI
BstEII BstPI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoNI EcoO65I
FseI FspI KpnI MluI MroI
NaeI NdeI NgoMIV NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SalI SexAI SfiI SgrAI SnaBI
SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 5224 GTAGAC GT^[AC][GT]AC
AccII 2644 CGCG CG^CG
AclI 742 AACGTT AA^CGTT
AflII 6447 CTTAAG C^TTAAG
AhdI 2408 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 6583 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 2408 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 2797 GAGCTC G[GA]GC[CT]^C
BbeI 5819 GGCGCC GGCGC^C
BfrI 6447 CTTAAG C^TTAAG
BsaBI 5900 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 6326 GTGCAG GTGCAG(16/14)
BsiEI 26 CGGTCG CG[GA][CT]^CG
BsmBI 4270 GAGACG CGTCTC(1/5)
Bsp1407I 1344 TGTACA T^GTACA
BspHI 2604 TCATGA T^CATGA
BspLU11I 6052 ACATGT A^CATGT
BsrGI 1344 TGTACA T^GTACA
BssSI 14 CTCGTG CACGAG(-5/-1)
BstBI 2543 TTCGAA TT^CGAA
BstUI 2644 CGCG CG^CG
DrdI 6422 GACCTTCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2408 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI 6516 GCGTC GACGC(5/10)
HincII 2193 GTTAAC GT[CT]^[GA]AC
HindII 2193 GTTAAC GT[CT]^[GA]AC
HpaI 2193 GTTAAC GTT^AAC
KasI 5819 GGCGCC G^GCGCC
KspI 2643 CCGCGG CCGC^GG
MamI 5900 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2644 CGCG CG^CG
NarI 5819 GGCGCC GG^CGCC
NspV 2543 TTCGAA TT^CGAA
PaeR7I 5456 CTCGAG C^TCGAG
PpuMI 6642 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 742 AACGTT AA^CGTT
RcaI 2604 TCATGA T^CATGA
SacI 2797 GAGCTC GAGCT^C
SacII 2643 CCGCGG CCGC^GG
SapI 2933 GCTCTTC GCTCTTC(1/4)
SbfI 7212 CCTGCAGG CCTGCA^GG
SfoI 5819 GGCGCC GGC^GCC
SfuI 2543 TTCGAA TT^CGAA
SmaI 6082 CCCGGG CCC^GGG
SpeI 2259 ACTAGT A^CTAGT
SphI 3635 GCATGC GCATG^C
Sse8387I 7212 CCTGCAGG CCTGCA^GG
SspBI 1344 TGTACA T^GTACA
StuI 241 AGGCCT AGG^CCT
XhoI 5456 CTCGAG C^TCGAG
XmaCI 6082 CCCGGG C^CCGGG
XmaI 6082 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 5819 GGCGCC G[GA]^CG[CT]C
6515 GGCGTC
AflIII 568 ACACGT A^C[GA][CT]GT
6052 ACATGT
Alw44I 3308 GTGCAC G^TGCAC
4017 GTGCAC
AocI 1123 CCTGAGG CC^T[ACGT]AGG
2023 CCTCAGG
Aor51HI 5345 AGCGCT AGC^GCT
7121 AGCGCT
ApaLI 3308 GTGCAC G^TGCAC
4017 GTGCAC
AvaI 5456 CTCGAG C^[CT]CG[GA]G
6082 CCCGGG
BanI 3436 GGTGCC G^G[CT][GA]CC
5819 GGCGCC
BciVI 1166 GGATAC GTATCC(6/5)
7636 GTATCC
BlpI 2048 GCTTAGC GC^T[ACGT]AGC
3664 GCTCAGC
Bpu1102I 2048 GCTTAGC GC^T[ACGT]AGC
3664 GCTCAGC
BsaHI 5819 GGCGCC G[GA]^CG[CT]C
6515 GGCGTC
BsaWI 24 TCCGGT [AT]^CCGG[AT]
4467 TCCGGT
BsoBI 5456 CTCGAG C^[CT]CG[GA]G
6082 CCCGGG
BsrFI 4040 ACCGGC [GA]^CCGG[CT]
4684 GCCGGT
Bsu36I 1123 CCTGAGG CC^T[ACGT]AGG
2023 CCTCAGG
CelII 2048 GCTTAGC GC^T[ACGT]AGC
3664 GCTCAGC
Cfr10I 4040 ACCGGC [GA]^CCGG[CT]
4684 GCCGGT
DraI 690 TTTAAA TTT^AAA
5100 TTTAAA
DraII 320 AGGGCCT [GA]G^G[ACGT]CC[CT]
6642 AGGTCCT
Eco47III 5345 AGCGCT AGC^GCT
7121 AGCGCT
Eco81I 1123 CCTGAGG CC^T[ACGT]AGG
2023 CCTCAGG
EcoO109I 320 AGGGCCT [GA]G^G[ACGT]CC[CT]
6642 AGGTCCT
FauI 1702 CCCGC CCCGC(4/6)
4649 CCCGC
Hin1I 5819 GGCGCC G[GA]^CG[CT]C
6515 GGCGTC
MfeI 2732 CAATTG C^AATTG
7320 CAATTG
MunI 2732 CAATTG C^AATTG
7320 CAATTG
NcoI 524 CCATGG C^CATGG
2967 CCATGG
SspI 996 AATATT AAT^ATT
4168 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AspHI
AvaII BalI BbsI BbvI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraIII
DsaI EaeI EarI EcoRI EcoRII
EcoRV EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI