(Circular) (Six-base) MAPSORT of: pFN21AE3824.seq Check: 7011 from: 1 to: 12367 pFN21AE3824 12367 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 7484 7484 Size: 12367 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 10177 278 Size: 53 83 186 936 8641 2468 Fragments arranged by size: 8641 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7230 9710 1937 Size: 5293 2480 4594 Fragments arranged by size: 5293 4594 2480 Acc65I G'GTAC_C Cuts at: 9688 9688 Size: 12367 AclI AA'CG_TT Cuts at: 1563 2748 10494 10867 1563 Size: 1185 7746 373 3063 Fragments arranged by size: 7746 3063 1185 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2634 4163 4493 5238 5594 6213 6924 6929 Size: 1002 1529 330 745 356 619 711 5 Cuts at: 6929 8130 9504 10430 11814 12082 1632 Size: 1201 1374 926 1384 268 1917 Fragments arranged by size: 1917 1529 1384 1374 1201 1002 926 745 711 619 356 330 268 5 AfeI AGC'GCT Cuts at: 7352 9128 7352 Size: 1776 10591 AflII C'TTAA_G Cuts at: 820 1017 8452 820 Size: 197 7435 4735 Fragments arranged by size: 7435 4735 197 AflIII A'CryG_T Cuts at: 1203 2573 8057 11266 12243 1203 Size: 1370 5484 3209 977 1327 Fragments arranged by size: 5484 3209 1370 1327 977 AgeI A'CCGG_T Cuts at: 11198 11198 Size: 12367 AhdI GACnn_n'nnGTC Cuts at: 4418 11096 4418 Size: 6678 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 12367 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 12367 AlwNI CAG_nnn'CTG Cuts at: 8593 10110 11682 8593 Size: 1517 1572 9278 Fragments arranged by size: 9278 1572 1517 ApaLI G'TGCA_C Cuts at: 5313 6022 10424 11580 5313 Size: 709 4402 1156 6100 Fragments arranged by size: 6100 4402 1156 709 ApoI r'AATT_y Cuts at: 1493 2128 2394 2433 2552 2684 2950 3036 3729 Size: 635 266 39 119 132 266 86 693 Cuts at: 3729 3760 5802 6341 6709 7067 7454 7700 9066 Size: 31 2042 539 368 358 387 246 1366 Cuts at: 9066 9087 9676 9902 10054 12163 1493 Size: 21 589 226 152 2109 1697 Fragments arranged by size: 2109 2042 1697 1366 693 635 589 539 387 368 358 266 266 246 226 152 132 119 86 39 31 21 AseI AT'TA_AT Cuts at: 160 1032 2730 4149 6177 6389 6498 10921 160 Size: 872 1698 1419 2028 212 109 4423 1606 Fragments arranged by size: 4423 2028 1698 1606 1419 872 212 109 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12367 AvaI C'yCGr_G Cuts at: 1870 1943 7461 8087 9692 1870 Size: 73 5518 626 1605 4545 Fragments arranged by size: 5518 4545 1605 626 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 7186 7219 7186 Size: 33 12334 BamHI G'GATC_C Cuts at: 9697 10064 9697 Size: 367 12000 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5441 7824 9688 11143 618 Size: 325 217 627 3654 2383 1864 1455 1842 Fragments arranged by size: 3654 2383 1864 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4806 9686 1391 Size: 3415 4880 4072 Fragments arranged by size: 4880 4072 3415 BbeI G_GCGC'C Cuts at: 7828 7828 Size: 12367 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3206 4449 5819 5850 8588 8736 8883 Size: 971 1307 1243 1370 31 2738 148 147 Cuts at: 8883 9295 928 Size: 412 4000 Fragments arranged by size: 4000 2738 1370 1307 1243 971 412 148 147 31 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 10558 10592 1903 Size: 34 8621 34 3678 Fragments arranged by size: 8621 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3165 9652 10259 11469 3165 Size: 6487 607 1210 4063 Fragments arranged by size: 6487 4063 1210 607 BclI T'GATC_A Cuts at: 1548 2238 3400 5581 7162 7795 8130 8464 1548 Size: 690 1162 2181 1581 633 335 334 5451 Fragments arranged by size: 5451 2181 1581 1162 690 633 335 334 BfrBI ATG'CAT Cuts at: 7307 8002 8222 7307 Size: 695 220 11452 Fragments arranged by size: 11452 695 220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 10978 136 Size: 107 122 71 631 605 9306 1525 Fragments arranged by size: 9306 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4375 7170 9232 12362 4375 Size: 2795 2062 3130 4380 Fragments arranged by size: 4380 3130 2795 2062 BlpI GC'TnA_GC Cuts at: 4054 5670 9784 4054 Size: 1616 4114 6637 Fragments arranged by size: 6637 4114 1616 Bme1580I G_kGCm'C Cuts at: 1790 3598 5317 5608 6026 6996 8327 10428 11584 Size: 1808 1719 291 418 970 1331 2101 1156 Cuts at: 11584 1790 Size: 2573 Fragments arranged by size: 2573 2101 1808 1719 1331 1156 970 418 291 BmgBI CAC'GTC Cuts at: 1318 2576 1318 Size: 1258 11109 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 7423 9332 10098 Size: 416 53 83 399 24 6006 1909 766 Cuts at: 10098 11056 442 Size: 958 1753 Fragments arranged by size: 6006 1909 1753 958 766 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12367 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3789 3789 Size: 12367 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2622 5046 8339 9441 11027 1239 Size: 1383 2424 3293 1102 1586 2579 Fragments arranged by size: 3293 2579 2424 1586 1383 1102 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2581 3488 8479 8515 10492 11357 11655 2581 Size: 907 4991 36 1977 865 298 3293 Fragments arranged by size: 4991 3293 1977 907 865 298 36 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3894 4725 9336 11030 882 Size: 617 2395 831 4611 1694 2219 Fragments arranged by size: 4611 2395 2219 1694 831 617 BsaAI yAC'GTr Cuts at: 493 1204 10158 493 Size: 711 8954 2702 Fragments arranged by size: 8954 2702 711 BsaBI GATnn'nnATC Cuts at: 7909 12361 7909 Size: 4452 7915 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7825 8521 10174 10556 Size: 53 83 186 936 6292 696 1653 382 Cuts at: 10556 11191 275 Size: 635 1451 Fragments arranged by size: 6292 1653 1451 936 696 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2029 6472 10800 11198 11472 11619 12030 2029 Size: 4443 4328 398 274 147 411 2366 Fragments arranged by size: 4443 4328 2366 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3125 3155 3342 3372 3608 Size: 30 165 30 1722 30 187 30 236 Cuts at: 3608 3638 4703 4733 4945 4975 5112 5142 1178 Size: 30 1065 30 212 30 137 30 8403 Fragments arranged by size: 8403 1722 1065 236 212 187 165 137 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3146 4017 4370 8073 8754 9446 9449 1186 Size: 1960 871 353 3703 681 692 3 4104 Fragments arranged by size: 4104 3703 1960 871 692 681 353 3 BseYI C'CCAG_C Cuts at: 3934 8925 9565 11570 12066 3934 Size: 4991 640 2005 496 4235 Fragments arranged by size: 4991 4235 2005 640 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 8352 1163 Size: 573 6616 5178 Fragments arranged by size: 6616 5178 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 9817 10578 11606 1540 Size: 275 186 33 7783 761 1028 2301 Fragments arranged by size: 7783 2301 1028 761 275 186 33 BsiHKAI G_wGCw'C Cuts at: 4027 4806 5317 6026 6095 7611 9613 10428 10513 Size: 779 511 709 69 1516 2002 815 85 Cuts at: 10513 11584 4027 Size: 1071 4810 Fragments arranged by size: 4810 2002 1516 1071 815 779 709 511 85 69 BsmI GAATG_Cn' Cuts at: 3067 4069 4643 5422 5616 7849 8016 8742 9878 Size: 1002 574 779 194 2233 167 726 1136 Cuts at: 9878 9971 3067 Size: 93 5463 Fragments arranged by size: 5463 2233 1136 1002 779 726 574 194 167 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6269 6269 Size: 12367 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3598 4027 4806 5317 5608 6026 6095 Size: 399 1808 429 779 511 291 418 69 Cuts at: 6095 6996 7611 8327 9613 9686 10428 10513 11584 Size: 901 615 716 1286 73 742 85 1071 Cuts at: 11584 1391 Size: 2174 Fragments arranged by size: 2174 1808 1286 1071 901 779 742 716 615 511 429 418 399 291 85 73 69 BspEI T'CCGG_A Cuts at: 12030 12030 Size: 12367 BspHI T'CATG_A Cuts at: 4609 10256 11986 4609 Size: 5647 1730 4990 Fragments arranged by size: 5647 4990 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2206 7484 8617 8965 9722 844 Size: 1362 5278 1133 348 757 3489 Fragments arranged by size: 5278 3489 1362 1133 757 348 BsrBI CCG'CTC Cuts at: 1394 1823 10254 1394 Size: 429 8431 3507 Fragments arranged by size: 8431 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2545 2792 5833 6838 8728 9232 9355 Size: 1183 1297 247 3041 1005 1890 504 123 Cuts at: 9355 10862 11036 65 Size: 1507 174 1396 Fragments arranged by size: 3041 1890 1507 1396 1297 1183 1005 504 247 174 123 BsrFI r'CCGG_y Cuts at: 1700 1810 6045 6689 11011 11198 1700 Size: 110 4235 644 4322 187 2869 Fragments arranged by size: 4322 4235 2869 644 187 110 BsrGI T'GTAC_A Cuts at: 96 3349 96 Size: 3253 9114 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12367 BssSI C'ACGA_G Cuts at: 2019 10427 11439 2019 Size: 8408 1012 2947 Fragments arranged by size: 8408 2947 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 12367 BstBI TT'CG_AA Cuts at: 4549 11171 4549 Size: 6622 5745 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 12367 BstXI CCAn_nnnn'nTGG Cuts at: 2661 4117 7667 7766 9512 9545 2661 Size: 1456 3550 99 1746 33 5483 Fragments arranged by size: 5483 3550 1746 1456 99 33 BstYI r'GATC_y Cuts at: 1972 2641 2671 3292 3648 4138 4375 4828 4958 Size: 669 30 621 356 490 237 453 130 Cuts at: 4958 7170 9016 9232 9515 9697 10064 10449 10466 Size: 2212 1846 216 283 182 367 385 17 Cuts at: 10466 11918 12004 12016 12362 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2212 1977 1846 1452 669 621 490 453 385 367 356 346 283 237 216 182 130 86 30 17 12 Bsu36I CC'TnA_GG Cuts at: 3129 4029 3129 Size: 900 11467 BtgI C'CryG_G Cuts at: 513 1065 2529 4648 4972 8194 513 Size: 552 1464 2119 324 3222 4686 Fragments arranged by size: 4686 3222 2119 1464 552 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2091 487 Size: 683 56 865 10763 Fragments arranged by size: 10763 865 683 56 BtsI GCAGTG_nn' Cuts at: 2418 5039 5595 9264 9637 9883 10677 10697 2418 Size: 2621 556 3669 373 246 794 20 4088 Fragments arranged by size: 4088 3669 2621 794 556 373 246 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 12367 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 12332 DraI TTT'AAA Cuts at: 2697 7107 9671 2697 Size: 4410 2564 5393 Fragments arranged by size: 5393 4410 2564 DraIII CAC_nnn'GTG Cuts at: 3523 4494 9389 3523 Size: 971 4895 6501 Fragments arranged by size: 6501 4895 971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8433 11374 809 Size: 507 7117 2941 1802 Fragments arranged by size: 7117 2941 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2220 2484 9601 Size: 54 1425 50 275 18 390 264 7117 Cuts at: 9601 9814 10703 12332 8 Size: 213 889 1629 43 Fragments arranged by size: 7117 1629 1425 889 390 275 264 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 9814 1537 Size: 275 8002 4090 Fragments arranged by size: 8002 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2811 3132 4945 6267 8734 8772 10297 1353 Size: 1458 321 1813 1322 2467 38 1525 3423 Fragments arranged by size: 3423 2467 1813 1525 1458 1322 321 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 10954 11328 11474 387 Size: 1064 138 9365 374 146 1280 Fragments arranged by size: 9365 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4804 4804 Size: 12367 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2622 2634 4163 4493 5046 5238 5594 Size: 393 990 12 1529 330 553 192 356 Cuts at: 5594 6213 6924 6929 8130 8339 9441 9504 10430 Size: 619 711 5 1201 209 1102 63 926 Cuts at: 10430 11027 11814 12082 1239 Size: 597 787 268 1524 Fragments arranged by size: 1529 1524 1201 1102 990 926 787 711 619 597 553 393 356 330 268 209 192 63 12 5 EcoO109I rG'GnC_Cy Cuts at: 2326 8648 2326 Size: 6322 6045 EcoRI G'AATT_C Cuts at: 6709 9066 9087 9676 10054 6709 Size: 2357 21 589 378 9022 Fragments arranged by size: 9022 2357 589 378 21 EcoRV GAT'ATC Cuts at: 3090 3574 5679 6863 3090 Size: 484 2105 1184 8594 Fragments arranged by size: 8594 2105 1184 484 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3992 4177 3992 Size: 185 12182 FspI TGC'GCA Cuts at: 10873 10873 Size: 12367 HaeII r_GCGC'y Cuts at: 1678 3755 7354 7828 9130 11514 1678 Size: 2077 3599 474 1302 2384 2531 Fragments arranged by size: 3599 2531 2384 2077 1302 474 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2057 2089 2633 2665 3466 Size: 32 135 32 485 32 544 32 801 Cuts at: 3466 3498 4404 4436 4703 4735 4746 4778 5318 Size: 32 906 32 267 32 11 32 540 Cuts at: 5318 5350 6785 6817 11008 11040 11082 11114 1373 Size: 32 1435 32 4191 32 42 32 2626 Fragments arranged by size: 4191 2626 1435 906 801 544 540 485 267 135 42 32 32 32 32 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 4200 9711 9957 669 Size: 988 281 2262 5511 246 3079 Fragments arranged by size: 5511 3079 2262 988 281 246 HindIII A'AGCT_T Cuts at: 748 5471 5520 7101 7110 8455 9411 748 Size: 4723 49 1581 9 1345 956 3704 Fragments arranged by size: 4723 3704 1581 1345 956 49 9 HpaI GTT'AAC Cuts at: 4200 9957 4200 Size: 5757 6610 KasI G'GCGC_C Cuts at: 7824 7824 Size: 12367 KpnI G_GTAC'C Cuts at: 9692 9692 Size: 12367 MfeI C'AATT_G Cuts at: 4737 9325 9966 4737 Size: 4588 641 7138 Fragments arranged by size: 7138 4588 641 MluI A'CGCG_T Cuts at: 12243 12243 Size: 12367 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2014 2113 2208 3410 5927 6628 11481 11665 Size: 709 99 95 1202 2517 701 4853 184 Cuts at: 11665 1305 Size: 2007 Fragments arranged by size: 4853 2517 2007 1202 709 701 184 99 95 MscI TGG'CCA Cuts at: 10 64 1489 1832 2222 2486 9603 10 Size: 54 1425 343 390 264 7117 2774 Fragments arranged by size: 7117 2774 1425 390 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 2578 3117 3555 3831 4728 4986 5635 Size: 648 1412 539 438 276 897 258 649 Cuts at: 5635 5752 9160 9543 10325 10684 10843 518 Size: 117 3408 383 782 359 159 2042 Fragments arranged by size: 3408 2042 1412 897 782 649 648 539 438 383 359 276 258 159 117 MspA1I CmG'CkG Cuts at: 1606 3152 4012 4650 6458 8707 8715 9783 10460 Size: 1546 860 638 1808 2249 8 1068 677 Cuts at: 10460 11608 11853 1606 Size: 1148 245 2120 Fragments arranged by size: 2249 2120 1808 1546 1148 1068 860 677 638 245 8 NarI GG'CG_CC Cuts at: 7825 7825 Size: 12367 NcoI C'CATG_G Cuts at: 513 1065 2529 4972 513 Size: 552 1464 2443 7908 Fragments arranged by size: 7908 2443 1464 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 12367 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12367 NotI GC'GGCC_GC Cuts at: 9814 9814 Size: 12367 NsiI A_TGCA'T Cuts at: 7309 8004 8224 7309 Size: 695 220 11452 Fragments arranged by size: 11452 695 220 NspI r_CATG'y Cuts at: 1132 1229 2352 5644 8061 9725 11270 1132 Size: 97 1123 3292 2417 1664 1545 2229 Fragments arranged by size: 3292 2417 2229 1664 1545 1123 97 PasI CC'CwG_GG Cuts at: 1348 6843 1348 Size: 5495 6872 PciI A'CATG_T Cuts at: 8057 11266 8057 Size: 3209 9158 PflMI CCAn_nnn'nTGG Cuts at: 1108 6647 8211 9299 1108 Size: 5539 1564 1088 4176 Fragments arranged by size: 5539 4176 1564 1088 PfoI T'CCnGG_A Cuts at: 2968 4963 8422 2968 Size: 1995 3459 6913 Fragments arranged by size: 6913 3459 1995 PmeI GTTT'AAAC Cuts at: 9671 9671 Size: 12367 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10436 10468 11949 11981 10436 Size: 32 1481 32 10822 Fragments arranged by size: 10822 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 8648 8648 Size: 12367 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 12367 PsiI TTA'TAA Cuts at: 9937 9937 Size: 12367 PstI C_TGCA'G Cuts at: 830 4837 8885 9222 9719 830 Size: 4007 4048 337 497 3478 Fragments arranged by size: 4048 4007 3478 497 337 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12367 PvuII CAG'CTG Cuts at: 3152 4012 6458 8707 3152 Size: 860 2446 2249 6812 Fragments arranged by size: 6812 2446 2249 860 SacI G_AGCT'C Cuts at: 4806 4806 Size: 12367 SacII CC_GC'GG Cuts at: 4651 4651 Size: 12367 SalI G'TCGA_C Cuts at: 1936 9709 1936 Size: 7773 4594 SapI GCTCTTCn'nnn_ Cuts at: 4945 4945 Size: 12367 SbfI CC_TGCA'GG Cuts at: 9222 9719 9222 Size: 497 11870 ScaI AGT'ACT Cuts at: 2825 5130 8484 10615 12327 2825 Size: 2305 3354 2131 1712 2865 Fragments arranged by size: 3354 2865 2305 2131 1712 SfcI C'TryA_G Cuts at: 826 1045 3567 4371 4833 6428 8166 8881 9218 Size: 219 2522 804 462 1595 1738 715 337 Cuts at: 9218 9715 10850 11531 11722 826 Size: 497 1135 681 191 1471 Fragments arranged by size: 2522 1738 1595 1471 1135 804 715 681 497 462 337 219 191 SfoI GGC'GCC Cuts at: 7826 7826 Size: 12367 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12367 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12367 SmaI CCC'GGG Cuts at: 1872 8089 9694 1872 Size: 6217 1605 4545 Fragments arranged by size: 6217 4545 1605 SmlI C'TyrA_G Cuts at: 820 1017 1943 2596 3467 7461 8452 8458 8494 Size: 197 926 653 871 3994 991 6 36 Cuts at: 8494 10471 11372 11634 820 Size: 1977 901 262 1553 Fragments arranged by size: 3994 1977 1553 991 926 901 871 653 262 197 36 6 SnaBI TAC'GTA Cuts at: 493 10158 493 Size: 9665 2702 SpeI A'CTAG_T Cuts at: 152 4264 152 Size: 4112 8255 SphI G_CATG'C Cuts at: 1132 5644 9725 1132 Size: 4512 4081 3774 Fragments arranged by size: 4512 4081 3774 SspI AAT'ATT Cuts at: 5 52 3003 6175 10291 5 Size: 47 2951 3172 4116 2081 Fragments arranged by size: 4116 3172 2951 2081 47 StuI AGG'CCT Cuts at: 1517 2248 1517 Size: 731 11636 StyI C'CwwG_G Cuts at: 513 1065 2529 4402 4972 6295 7177 9657 9806 Size: 552 1464 1873 570 1323 882 2480 149 Cuts at: 9806 513 Size: 3074 Fragments arranged by size: 3074 2480 1873 1464 1323 882 570 552 149 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2245 6676 7592 9114 10394 10411 10564 Size: 1255 665 4431 916 1522 1280 17 153 Cuts at: 10564 10749 11194 325 Size: 185 445 1498 Fragments arranged by size: 4431 1522 1498 1280 1255 916 665 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2823 3349 5128 Size: 274 80 33 51 1443 846 526 1779 Cuts at: 5128 5380 6918 7776 8016 8482 9312 10613 12325 Size: 252 1538 858 240 466 830 1301 1712 Cuts at: 12325 96 Size: 138 Fragments arranged by size: 1779 1712 1538 1443 1301 858 846 830 526 466 274 252 240 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3125 3157 3633 3665 4945 4977 8694 8726 12327 Size: 32 476 32 1280 32 3717 32 3601 Cuts at: 12327 12359 3125 Size: 32 3133 Fragments arranged by size: 3717 3601 3133 1280 476 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 12367 XbaI T'CTAG_A Cuts at: 9703 9703 Size: 12367 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2063 2564 5005 5201 6034 6670 7667 7766 8569 Size: 501 2441 196 833 636 997 99 803 Cuts at: 8569 2063 Size: 5861 Fragments arranged by size: 5861 2441 997 833 803 636 501 196 99 XhoI C'TCGA_G Cuts at: 1943 7461 1943 Size: 5518 6849 XmaI C'CCGG_G Cuts at: 1870 8087 9692 1870 Size: 6217 1605 4545 Fragments arranged by size: 6217 4545 1605 XmnI GAAnn'nnTTC Cuts at: 2847 3678 4545 6198 7013 8313 10496 2847 Size: 831 867 1653 815 1300 2183 4718 Fragments arranged by size: 4718 2183 1653 1300 867 831 815 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 10175 276 Size: 53 83 186 936 8641 2468 Fragments arranged by size: 8641 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbvCI Bpu10I BsiWI BstZ17I EcoNI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI