Restriction map for
pFN21AE3824
(Linear) (Six-base) MAPSORT of: KIEE3824.seq Check: 8543 from: 1 to: 7662
KIEE3824.orf
With 182 enzymes: SgfI *
November 2, 2012 16:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 5479 7662
Size: 5479 2183
AccI GT'mk_AC
Cuts at: 0 5225 7662
Size: 5225 2437
AclI AA'CG_TT
Cuts at: 0 743 7662
Size: 743 6919
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 2158 2488 3233 3589 4208 4919 4924
Size: 629 1529 330 745 356 619 711 5
Cuts at: 4924 6125 7499 7662
Size: 1201 1374 163
Fragments arranged by size:
1529 1374 1201 745 711 629 619 356
330 163 5
AfeI AGC'GCT
Cuts at: 0 5347 7123 7662
Size: 5347 1776 539
Fragments arranged by size:
5347 1776 539
AflII C'TTAA_G
Cuts at: 0 6447 7662
Size: 6447 1215
AflIII A'CryG_T
Cuts at: 0 568 6052 7662
Size: 568 5484 1610
Fragments arranged by size:
5484 1610 568
AhdI GACnn_n'nnGTC
Cuts at: 0 2413 7662
Size: 2413 5249
AlwNI CAG_nnn'CTG
Cuts at: 0 6588 7662
Size: 6588 1074
ApaLI G'TGCA_C
Cuts at: 0 3308 4017 7662
Size: 3308 709 3645
Fragments arranged by size:
3645 3308 709
ApoI r'AATT_y
Cuts at: 0 123 389 428 547 679 945 1031 1724
Size: 123 266 39 119 132 266 86 693
Cuts at: 1724 1755 3797 4336 4704 5062 5449 5695 7061
Size: 31 2042 539 368 358 387 246 1366
Cuts at: 7061 7082 7662
Size: 21 580
Fragments arranged by size:
2042 1366 693 580 539 387 368 358
266 266 246 132 123 119 86 39
31 21
AseI AT'TA_AT
Cuts at: 0 725 2144 4172 4384 4493 7662
Size: 725 1419 2028 212 109 3169
Fragments arranged by size:
3169 2028 1419 725 212 109
AvaI C'yCGr_G
Cuts at: 0 5456 6082 7662
Size: 5456 626 1580
Fragments arranged by size:
5456 1580 626
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5181 5214 7662
Size: 5181 33 2448
Fragments arranged by size:
5181 2448 33
BanI G'GyrC_C
Cuts at: 0 3436 5819 7662
Size: 3436 2383 1843
Fragments arranged by size:
3436 2383 1843
BanII G_rGCy'C
Cuts at: 0 2801 7662
Size: 2801 4861
BbeI G_GCGC'C
Cuts at: 0 5823 7662
Size: 5823 1839
BbsI GAAGACnn'nnnn_
Cuts at: 0 1201 2444 3814 3845 6583 6731 6878 7290
Size: 1201 1243 1370 31 2738 148 147 412
Cuts at: 7290 7662
Size: 372
Fragments arranged by size:
2738 1370 1243 1201 412 372 148 147
31
BciVI GTATCCnnnnn_n'
Cuts at: 0 1160 7647 7662
Size: 1160 6487 15
Fragments arranged by size:
6487 1160 15
BclI T'GATC_A
Cuts at: 0 233 1395 3576 5157 5790 6125 6459 7662
Size: 233 1162 2181 1581 633 335 334 1203
Fragments arranged by size:
2181 1581 1203 1162 633 335 334 233
BfrBI ATG'CAT
Cuts at: 0 5302 5997 6217 7662
Size: 5302 695 220 1445
Fragments arranged by size:
5302 1445 695 220
BglII A'GATC_T
Cuts at: 0 2370 5165 7227 7662
Size: 2370 2795 2062 435
Fragments arranged by size:
2795 2370 2062 435
BlpI GC'TnA_GC
Cuts at: 0 2049 3665 7662
Size: 2049 1616 3997
Fragments arranged by size:
3997 2049 1616
Bme1580I G_kGCm'C
Cuts at: 0 1593 3312 3603 4021 4991 6322 7662
Size: 1593 1719 291 418 970 1331 1340
Fragments arranged by size:
1719 1593 1340 1331 970 418 291
BmgBI CAC'GTC
Cuts at: 0 571 7662
Size: 571 7091
BmrI ACTGGGnnnn_n'
Cuts at: 0 5418 7327 7662
Size: 5418 1909 335
Fragments arranged by size:
5418 1909 335
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1784 7662
Size: 1784 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 3041 6334 7436 7662
Size: 617 2424 3293 1102 226
Fragments arranged by size:
3293 2424 1102 617 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 1483 6474 6510 7662
Size: 576 907 4991 36 1152
Fragments arranged by size:
4991 1152 907 576 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 1889 2720 7331 7662
Size: 1889 831 4611 331
Fragments arranged by size:
4611 1889 831 331
BsaBI GATnn'nnATC
Cuts at: 0 5904 7662
Size: 5904 1758
BsaHI Gr'CG_yC
Cuts at: 0 5820 6516 7662
Size: 5820 696 1146
Fragments arranged by size:
5820 1146 696
BsaWI w'CCGG_w
Cuts at: 0 24 4467 7662
Size: 24 4443 3195
Fragments arranged by size:
4443 3195 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1120 1150 1337 1367 1603 1633 2698 2728
Size: 1120 30 187 30 236 30 1065 30
Cuts at: 2728 2940 2970 3107 3137 7662
Size: 212 30 137 30 4525
Fragments arranged by size:
4525 1120 1065 236 212 187 137 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1141 2012 2365 6068 6749 7441 7444 7662
Size: 1141 871 353 3703 681 692 3 218
Fragments arranged by size:
3703 1141 871 692 681 353 218 3
BseYI C'CCAG_C
Cuts at: 0 1929 6920 7560 7662
Size: 1929 4991 640 102
Fragments arranged by size:
4991 1929 640 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6347 7662
Size: 6347 1315
BsiEI CG_ry'CG
Cuts at: 0 29 7662
Size: 29 7633
BsiHKAI G_wGCw'C
Cuts at: 0 2022 2801 3312 4021 4090 5606 7608 7662
Size: 2022 779 511 709 69 1516 2002 54
Fragments arranged by size:
2022 2002 1516 779 709 511 69 54
BsmI GAATG_Cn'
Cuts at: 0 1062 2064 2638 3417 3611 5844 6011 6737
Size: 1062 1002 574 779 194 2233 167 726
Cuts at: 6737 7662
Size: 925
Fragments arranged by size:
2233 1062 1002 925 779 726 574 194
167
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4264 7662
Size: 4264 3398
Bsp1286I G_dGCh'C
Cuts at: 0 1593 2022 2801 3312 3603 4021 4090 4991
Size: 1593 429 779 511 291 418 69 901
Cuts at: 4991 5606 6322 7608 7662
Size: 615 716 1286 54
Fragments arranged by size:
1593 1286 901 779 716 615 511 429
418 291 69 54
BspHI T'CATG_A
Cuts at: 0 2604 7662
Size: 2604 5058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 5479 6612 6960 7662
Size: 201 5278 1133 348 702
Fragments arranged by size:
5278 1133 702 348 201
BsrDI GCAATG_nn'
Cuts at: 0 540 787 3828 4833 6723 7227 7350 7662
Size: 540 247 3041 1005 1890 504 123 312
Fragments arranged by size:
3041 1890 1005 540 504 312 247 123
BsrFI r'CCGG_y
Cuts at: 0 4040 4684 7662
Size: 4040 644 2978
Fragments arranged by size:
4040 2978 644
BsrGI T'GTAC_A
Cuts at: 0 1344 7662
Size: 1344 6318
BssSI C'ACGA_G
Cuts at: 0 14 7662
Size: 14 7648
BstBI TT'CG_AA
Cuts at: 0 2544 7662
Size: 2544 5118
BstXI CCAn_nnnn'nTGG
Cuts at: 0 656 2112 5662 5761 7507 7540 7662
Size: 656 1456 3550 99 1746 33 122
Fragments arranged by size:
3550 1746 1456 656 122 99 33
BstYI r'GATC_y
Cuts at: 0 636 666 1287 1643 2133 2370 2823 2953
Size: 636 30 621 356 490 237 453 130
Cuts at: 2953 5165 7011 7227 7510 7662
Size: 2212 1846 216 283 152
Fragments arranged by size:
2212 1846 636 621 490 453 356 283
237 216 152 130 30
Bsu36I CC'TnA_GG
Cuts at: 0 1124 2024 7662
Size: 1124 900 5638
Fragments arranged by size:
5638 1124 900
BtgI C'CryG_G
Cuts at: 0 524 2643 2967 6189 7662
Size: 524 2119 324 3222 1473
Fragments arranged by size:
3222 2119 1473 524 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 86 7662
Size: 86 7576
BtsI GCAGTG_nn'
Cuts at: 0 413 3034 3590 7259 7632 7662
Size: 413 2621 556 3669 373 30
Fragments arranged by size:
3669 2621 556 413 373 30
DraI TTT'AAA
Cuts at: 0 692 5102 7662
Size: 692 4410 2560
Fragments arranged by size:
4410 2560 692
DraIII CAC_nnn'GTG
Cuts at: 0 1518 2489 7384 7662
Size: 1518 971 4895 278
Fragments arranged by size:
4895 1518 971 278
DrdI GACnn_nn'nnGTC
Cuts at: 0 6428 7662
Size: 6428 1234
EaeI y'GGCC_r
Cuts at: 0 215 479 7596 7662
Size: 215 264 7117 66
Fragments arranged by size:
7117 264 215 66
EarI CTCTTCn'nnn_
Cuts at: 0 806 1127 2940 4262 6729 6767 7662
Size: 806 321 1813 1322 2467 38 895
Fragments arranged by size:
2467 1813 1322 895 806 321 38
EcoICRI GAG'CTC
Cuts at: 0 2799 7662
Size: 2799 4863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 629 2158 2488 3041 3233 3589 4208
Size: 617 12 1529 330 553 192 356 619
Cuts at: 4208 4919 4924 6125 6334 7436 7499 7662
Size: 711 5 1201 209 1102 63 163
Fragments arranged by size:
1529 1201 1102 711 619 617 553 356
330 209 192 163 63 12 5
EcoO109I rG'GnC_Cy
Cuts at: 0 321 6643 7662
Size: 321 6322 1019
Fragments arranged by size:
6322 1019 321
EcoRI G'AATT_C
Cuts at: 0 4704 7061 7082 7662
Size: 4704 2357 21 580
Fragments arranged by size:
4704 2357 580 21
EcoRV GAT'ATC
Cuts at: 0 1085 1569 3674 4858 7662
Size: 1085 484 2105 1184 2804
Fragments arranged by size:
2804 2105 1184 1085 484
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1987 2172 7662
Size: 1987 185 5490
Fragments arranged by size:
5490 1987 185
HaeII r_GCGC'y
Cuts at: 0 1750 5349 5823 7125 7662
Size: 1750 3599 474 1302 537
Fragments arranged by size:
3599 1750 1302 537 474
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 628 660 1461 1493 2399 2431
Size: 52 32 544 32 801 32 906 32
Cuts at: 2431 2698 2730 2741 2773 3313 3345 4780 4812
Size: 267 32 11 32 540 32 1435 32
Cuts at: 4812 7662
Size: 2850
Fragments arranged by size:
2850 1435 906 801 544 540 267 52
32 32 32 32 32 32 32 32
11
HincII GTy'rAC
Cuts at: 0 2195 7662
Size: 2195 5467
HindIII A'AGCT_T
Cuts at: 0 3466 3515 5096 5105 6450 7406 7662
Size: 3466 49 1581 9 1345 956 256
Fragments arranged by size:
3466 1581 1345 956 256 49 9
HpaI GTT'AAC
Cuts at: 0 2195 7662
Size: 2195 5467
KasI G'GCGC_C
Cuts at: 0 5819 7662
Size: 5819 1843
MfeI C'AATT_G
Cuts at: 0 2732 7320 7662
Size: 2732 4588 342
Fragments arranged by size:
4588 2732 342
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 108 203 1405 3922 4623 7662
Size: 9 99 95 1202 2517 701 3039
Fragments arranged by size:
3039 2517 1202 701 99 95 9
MscI TGG'CCA
Cuts at: 0 217 481 7598 7662
Size: 217 264 7117 64
Fragments arranged by size:
7117 264 217 64
MslI CAynn'nnrTG
Cuts at: 0 573 1112 1550 1826 2723 2981 3630 3747
Size: 573 539 438 276 897 258 649 117
Cuts at: 3747 7155 7538 7662
Size: 3408 383 124
Fragments arranged by size:
3408 897 649 573 539 438 383 276
258 124 117
MspA1I CmG'CkG
Cuts at: 0 1147 2007 2645 4453 6702 6710 7662
Size: 1147 860 638 1808 2249 8 952
Fragments arranged by size:
2249 1808 1147 952 860 638 8
NarI GG'CG_CC
Cuts at: 0 5820 7662
Size: 5820 1842
NcoI C'CATG_G
Cuts at: 0 524 2967 7662
Size: 524 2443 4695
Fragments arranged by size:
4695 2443 524
NsiI A_TGCA'T
Cuts at: 0 5304 5999 6219 7662
Size: 5304 695 220 1443
Fragments arranged by size:
5304 1443 695 220
NspI r_CATG'y
Cuts at: 0 347 3639 6056 7662
Size: 347 3292 2417 1606
Fragments arranged by size:
3292 2417 1606 347
PasI CC'CwG_GG
Cuts at: 0 4838 7662
Size: 4838 2824
PciI A'CATG_T
Cuts at: 0 6052 7662
Size: 6052 1610
PflMI CCAn_nnn'nTGG
Cuts at: 0 4642 6206 7294 7662
Size: 4642 1564 1088 368
Fragments arranged by size:
4642 1564 1088 368
PfoI T'CCnGG_A
Cuts at: 0 963 2958 6417 7662
Size: 963 1995 3459 1245
Fragments arranged by size:
3459 1995 1245 963
PpuMI rG'GwC_Cy
Cuts at: 0 6643 7662
Size: 6643 1019
PstI C_TGCA'G
Cuts at: 0 2832 6880 7217 7662
Size: 2832 4048 337 445
Fragments arranged by size:
4048 2832 445 337
PvuII CAG'CTG
Cuts at: 0 1147 2007 4453 6702 7662
Size: 1147 860 2446 2249 960
Fragments arranged by size:
2446 2249 1147 960 860
SacI G_AGCT'C
Cuts at: 0 2801 7662
Size: 2801 4861
SacII CC_GC'GG
Cuts at: 0 2646 7662
Size: 2646 5016
SapI GCTCTTCn'nnn_
Cuts at: 0 2940 7662
Size: 2940 4722
SbfI CC_TGCA'GG
Cuts at: 0 7217 7662
Size: 7217 445
ScaI AGT'ACT
Cuts at: 0 820 3125 6479 7662
Size: 820 2305 3354 1183
Fragments arranged by size:
3354 2305 1183 820
SfcI C'TryA_G
Cuts at: 0 1562 2366 2828 4423 6161 6876 7213 7662
Size: 1562 804 462 1595 1738 715 337 449
Fragments arranged by size:
1738 1595 1562 804 715 462 449 337
SfoI GGC'GCC
Cuts at: 0 5821 7662
Size: 5821 1841
SmaI CCC'GGG
Cuts at: 0 6084 7662
Size: 6084 1578
SmlI C'TyrA_G
Cuts at: 0 591 1462 5456 6447 6453 6489 7662
Size: 591 871 3994 991 6 36 1173
Fragments arranged by size:
3994 1173 991 871 591 36 6
SpeI A'CTAG_T
Cuts at: 0 2259 7662
Size: 2259 5403
SphI G_CATG'C
Cuts at: 0 3639 7662
Size: 3639 4023
SspI AAT'ATT
Cuts at: 0 998 4170 7662
Size: 998 3172 3492
Fragments arranged by size:
3492 3172 998
StuI AGG'CCT
Cuts at: 0 243 7662
Size: 243 7419
StyI C'CwwG_G
Cuts at: 0 524 2397 2967 4290 5172 7652 7662
Size: 524 1873 570 1323 882 2480 10
Fragments arranged by size:
2480 1873 1323 882 570 524 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 240 4671 5587 7109 7662
Size: 240 4431 916 1522 553
Fragments arranged by size:
4431 1522 916 553 240
TatI w'GTAC_w
Cuts at: 0 818 1344 3123 3375 4913 5771 6011 6477
Size: 818 526 1779 252 1538 858 240 466
Cuts at: 6477 7307 7662
Size: 830 355
Fragments arranged by size:
1779 1538 858 830 818 526 466 355
252 240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1120 1152 1628 1660 2940 2972 6689 6721
Size: 1120 32 476 32 1280 32 3717 32
Cuts at: 6721 7662
Size: 941
Fragments arranged by size:
3717 1280 1120 941 476 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 58 559 3000 3196 4029 4665 5662 5761
Size: 58 501 2441 196 833 636 997 99
Cuts at: 5761 6564 7662
Size: 803 1098
Fragments arranged by size:
2441 1098 997 833 803 636 501 196
99 58
XhoI C'TCGA_G
Cuts at: 0 5456 7662
Size: 5456 2206
XmaI C'CCGG_G
Cuts at: 0 6082 7662
Size: 6082 1580
XmnI GAAnn'nnTTC
Cuts at: 0 842 1673 2540 4193 5008 6308 7662
Size: 842 831 867 1653 815 1300 1354
Fragments arranged by size:
1653 1354 1300 867 842 831 815
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AflIII AhdI
AlwNI ApaLI ApoI AseI AvaI BaeI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI
MfeI MmeI MscI MslI MspA1I NarI NcoI NsiI
NspI PasI PciI PflMI PfoI PpuMI PstI PvuII
SacI SacII SapI SbfI ScaI SfcI SfoI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AgeI AleI AlfI AloI ApaI AscI
AsiSI AvrII BamHI BbvCI BcgI BglI BmtI Bpu10I
BsaAI BsiWI BspEI BsrBI BssHII BstAPI BstEII BstZ17I
ClaI CspCI EagI EciI EcoNI FseI FspI FspAI
KpnI MluI NaeI NdeI NgoMIV NheI NotI NruI
PacI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SanDI SexAI SfiI SgfI
SgrAI SnaBI SrfI SwaI Tth111I XbaI ZraI