Restriction map for
pFN21AE5838
##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AclI AflII Alw44I
AocI ApaLI AscI AseI AsnI
Asp718I AvrII BamHI BbeI BbvCI
BcgI BfrI BlnI BsiEI BsiWI
Bst1107I BstEII BstPI BstZ17I Bsu36I
CpoI EagI EclXI Eco52I Eco81I
EcoNI EcoO65I FseI KasI KpnI
KspI MluI NarI NheI NotI
NruI PacI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SexAI
SfoI SgrAI SmaI SnaBI SspI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1399 GTCGAC GT^[AC][GT]AC
AgeI 2558 ACCGGT A^CCGGT
Aor51HI 1730 AGCGCT AGC^GCT
AviII 2300 TGCGCA TGC^GCA
BanI 4608 GGTGCC G^G[CT][GA]CC
BclI 3443 TGATCA T^GATCA
Bsp1407I 1571 TGTACA T^GTACA
BspDI 3360 ATCGAT AT^CGAT
BsrGI 1571 TGTACA T^GTACA
BssHII 4899 GCGCGC G^CGCGC
BssSI 3502 CACGAG CACGAG(-5/-1)
BstBI 926 TTCGAA TT^CGAA
ClaI 3360 ATCGAT AT^CGAT
DraI 4069 TTTAAA TTT^AAA
DraIII 4656 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1730 AGCGCT AGC^GCT
EcoRV 3363 GATATC GAT^ATC
FbaI 3443 TGATCA T^GATCA
FspI 2300 TGCGCA TGC^GCA
HpaI 667 GTTAAC GTT^AAC
MfeI 4682 CAATTG C^AATTG
MunI 4682 CAATTG C^AATTG
NdeI 4937 CATATG CA^TATG
NspV 926 TTCGAA TT^CGAA
PinAI 2558 ACCGGT A^CCGGT
SalI 1399 GTCGAC G^TCGAC
SbfI 3949 CCTGCAGG CCTGCA^GG
ScaI 2816 AGTACT AGT^ACT
SfuI 926 TTCGAA TT^CGAA
SpeI 4102 ACTAGT A^CTAGT
Sse8387I 3949 CCTGCAGG CCTGCA^GG
SspBI 1571 TGTACA T^GTACA
XbaI 4533 TCTAGA T^CTAGA
XcmI 1991 CCAAGGCCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 128 TCCGGA T^CCGGA
1736 TCCGGA
AcyI 269 GGCGTC G[GA]^CG[CT]C
3871 GACGCC
AhdI 355 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5056 GACAGCCTGTC
ApaI 1588 GGGCCC GGGCC^C
2982 GGGCCC
AspEI 355 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5056 GACAGCCTGTC
BbrPI 2199 CACGTG CAC^GTG
2250 CACGTG
BciVI 2644 GGATAC GTATCC(6/5)
3650 GGATAC
BcnI 3513 CCCGG CC^[GC]GG
4095 CCGGG
BglI 1996 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3271 GCCATCATGGC
BglII 1418 AGATCT A^GATCT
4408 AGATCT
BlpI 1222 GCTCAGC GC^T[ACGT]AGC
1376 GCTTAGC
Bpu1102I 1222 GCTCAGC GC^T[ACGT]AGC
1376 GCTTAGC
BsaAI 2199 CACGTG [CT]AC^GT[GA]
2250 CACGTG
BsaBI 1665 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2712 GATCGTAATC
BsaHI 269 GGCGTC G[GA]^CG[CT]C
3871 GACGCC
BsaI 3205 GAGACC GGTCTC(1/5)
3961 GAGACC
BseAI 128 TCCGGA T^CCGGA
1736 TCCGGA
BsgI 3864 CTGCAC GTGCAG(16/14)
2253 GTGCAG
BsmBI 2875 CGTCTC CGTCTC(1/5)
2947 CGTCTC
BsmI 4441 GAATGC GAATGC(1/-1)
864 GCATTC
BspEI 128 TCCGGA T^CCGGA
1736 TCCGGA
BspLU11I 2276 ACATGT A^CATGT
3680 ACATGT
BspMI 1637 ACCTGC ACCTGC(4/8)
3952 GCAGGT
BsrBI 3843 CCGCTC CCGCTC(-3/-3)
3820 GAGCGG
CelII 1222 GCTCAGC GC^T[ACGT]AGC
1376 GCTTAGC
DrdI 2902 GACGGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4201 GACATCTATGTC
Eam1105I 355 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5056 GACAGCCTGTC
EcoRI 2333 GAATTC G^AATTC
2590 GAATTC
EcoT22I 862 ATGCAT ATGCA^T
3591 ATGCAT
FauI 1700 GCGGG CCCGC(4/6)
3822 GCGGG
HaeII 1730 AGCGCT [GA]GCGC^[CT]
3803 AGCGCC
HgaI 3871 GACGC GACGC(5/10)
270 GCGTC
Hin1I 269 GGCGTC G[GA]^CG[CT]C
3871 GACGCC
MamI 1665 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2712 GATCGTAATC
MroI 128 TCCGGA T^CCGGA
1736 TCCGGA
NaeI 2288 GCCGGC GCC^GGC
3267 GCCGGC
NciI 3513 CCCGG CC^[GC]GG
4095 CCGGG
NgoMIV 2288 GCCGGC G^CCGGC
3267 GCCGGC
NsiI 862 ATGCAT ATGCA^T
3591 ATGCAT
PaeR7I 725 CTCGAG C^TCGAG
3846 CTCGAG
PmaCI 2199 CACGTG CAC^GTG
2250 CACGTG
PmlI 2199 CACGTG CAC^GTG
2250 CACGTG
SfiI 1995 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3270 GGCCATCATGGCC
StuI 2036 AGGCCT AGG^CCT
2313 AGGCCT
XhoI 725 CTCGAG C^TCGAG
3846 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AspI AvaI AvaII BalI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII
XmnI