(Circular) (Six-base) MAPSORT of: pFN21AE5838.seq Check: 9887 from: 1 to: 9826 pFN21AE5838 9826 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5948 5948 Size: 9826 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7636 278 Size: 53 83 186 936 6100 2468 Fragments arranged by size: 6100 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3405 7169 1937 Size: 1468 3764 4594 Fragments arranged by size: 4594 3764 1468 Acc65I G'GTAC_C Cuts at: 7147 7147 Size: 9826 AclI AA'CG_TT Cuts at: 1563 7125 7953 8326 1563 Size: 5562 828 373 3063 Fragments arranged by size: 5562 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2198 2306 4205 4882 5440 5596 5652 6408 Size: 566 108 1899 677 558 156 56 756 Cuts at: 6408 7889 9273 9541 1632 Size: 1481 1384 268 1917 Fragments arranged by size: 1917 1899 1481 1384 756 677 566 558 268 156 108 56 AfeI AGC'GCT Cuts at: 3737 3737 Size: 9826 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9629 AflIII A'CryG_T Cuts at: 1203 4254 4281 5685 8725 9702 1203 Size: 3051 27 1404 3040 977 1327 Fragments arranged by size: 3051 3040 1404 1327 977 27 AgeI A'CCGG_T Cuts at: 4563 8657 4563 Size: 4094 5732 AhdI GACnn_n'nnGTC Cuts at: 2365 7066 8555 2365 Size: 4701 1489 3636 Fragments arranged by size: 4701 3636 1489 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9826 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4283 1184 Size: 3099 6727 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5615 5647 5615 Size: 32 9794 AlwNI CAG_nnn'CTG Cuts at: 3235 4081 4270 5485 6152 7569 9141 3235 Size: 846 189 1215 667 1417 1572 3920 Fragments arranged by size: 3920 1572 1417 1215 846 667 189 ApaI G_GGCC'C Cuts at: 3597 4991 3597 Size: 1394 8432 ApaLI G'TGCA_C Cuts at: 7883 9039 7883 Size: 1156 8670 ApoI r'AATT_y Cuts at: 1493 2077 4338 4595 7135 7361 7513 9622 1493 Size: 584 2261 257 2540 226 152 2109 1697 Fragments arranged by size: 2540 2261 2109 1697 584 257 226 152 AseI AT'TA_AT Cuts at: 160 1032 8380 160 Size: 872 7348 1606 Fragments arranged by size: 7348 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9826 AvaI C'yCGr_G Cuts at: 1870 1943 2730 5214 5851 7151 1870 Size: 73 787 2484 637 1300 4545 Fragments arranged by size: 4545 2484 1300 787 637 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6291 6324 6291 Size: 33 9793 BamHI G'GATC_C Cuts at: 7156 7523 7156 Size: 367 9459 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6613 7147 8602 618 Size: 325 217 627 4826 534 1455 1842 Fragments arranged by size: 4826 1842 1455 627 534 325 217 BanII G_rGCy'C Cuts at: 1391 2625 3597 3921 4125 4491 4991 5568 6234 Size: 1234 972 324 204 366 500 577 666 Cuts at: 6234 7145 1391 Size: 911 4072 Fragments arranged by size: 4072 1234 972 911 666 577 500 366 324 204 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2941 3206 5781 6193 928 Size: 971 1042 265 2575 412 4561 Fragments arranged by size: 4561 2575 1042 971 412 265 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 8017 8051 1903 Size: 34 50 34 5996 34 3678 Fragments arranged by size: 5996 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4643 5649 7718 8928 4643 Size: 1006 2069 1210 5541 Fragments arranged by size: 5541 2069 1210 1006 BclI T'GATC_A Cuts at: 1548 5448 1548 Size: 3900 5926 BfrBI ATG'CAT Cuts at: 2869 5598 2869 Size: 2729 7097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4007 5282 8437 Size: 107 122 71 631 605 2335 1275 3155 Cuts at: 8437 136 Size: 1525 Fragments arranged by size: 3155 2335 1525 1275 631 605 122 107 71 BglII A'GATC_T Cuts at: 3423 6413 9821 3423 Size: 2990 3408 3428 Fragments arranged by size: 3428 3408 2990 BlpI GC'TnA_GC Cuts at: 3228 3382 7243 3228 Size: 154 3861 5811 Fragments arranged by size: 5811 3861 154 Bme1580I G_kGCm'C Cuts at: 1790 3597 4991 7887 9043 1790 Size: 1807 1394 2896 1156 2573 Fragments arranged by size: 2896 2573 1807 1394 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9826 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2301 7557 8515 Size: 416 53 83 399 24 884 5256 958 Cuts at: 8515 442 Size: 1753 Fragments arranged by size: 5256 1753 958 884 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9826 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2573 2667 2685 2945 3482 3544 4213 4510 Size: 1334 94 18 260 537 62 669 297 Cuts at: 4510 5720 5726 5825 5843 6288 6732 8486 1239 Size: 1210 6 99 18 445 444 1754 2579 Fragments arranged by size: 2579 1754 1334 1210 669 537 445 444 297 260 99 94 62 18 18 6 Bpu10I CC'TnA_GC Cuts at: 2046 6089 2046 Size: 4043 5783 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2585 3841 4307 5552 6571 6666 7951 8816 9114 Size: 1256 466 1245 1019 95 1285 865 298 Cuts at: 9114 2585 Size: 3297 Fragments arranged by size: 3297 1285 1256 1245 1019 865 466 298 95 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5204 5960 8489 882 Size: 617 3705 756 2529 2219 Fragments arranged by size: 3705 2529 2219 756 617 BsaAI yAC'GTr Cuts at: 493 1204 4206 4257 7617 493 Size: 711 3002 51 3360 2702 Fragments arranged by size: 3360 3002 2702 711 51 BsaBI GATnn'nnATC Cuts at: 3674 4721 9820 3674 Size: 1047 5099 3680 Fragments arranged by size: 5099 3680 1047 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2275 5877 7633 8015 Size: 53 83 186 936 742 3602 1756 382 Cuts at: 8015 8650 275 Size: 635 1451 Fragments arranged by size: 3602 1756 1451 936 742 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2133 3741 4563 8259 8657 8931 9078 9489 2133 Size: 1608 822 3696 398 274 147 411 2470 Fragments arranged by size: 3696 2470 1608 822 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2484 2514 2907 2937 5672 Size: 30 165 30 1081 30 393 30 2735 Cuts at: 5672 5702 5957 5987 6383 6413 6714 6744 1178 Size: 30 255 30 396 30 301 30 4260 Fragments arranged by size: 4260 2735 1081 396 393 301 255 165 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2100 2492 3998 5120 5315 5978 6812 6941 Size: 914 392 1506 1122 195 663 834 129 Cuts at: 6941 7090 1186 Size: 149 3922 Fragments arranged by size: 3922 1506 1122 914 834 663 392 195 149 129 BseYI C'CCAG_C Cuts at: 2240 3796 6280 6782 9029 9525 2240 Size: 1556 2484 502 2247 496 2541 Fragments arranged by size: 2541 2484 2247 1556 502 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4279 5854 1163 Size: 573 2543 1575 5135 Fragments arranged by size: 5135 2543 1575 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7276 8037 9065 1540 Size: 275 186 5275 761 1028 2301 Fragments arranged by size: 5275 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3921 4125 4491 5568 5724 6234 7887 7972 9043 Size: 204 366 1077 156 510 1653 85 1071 Cuts at: 9043 3921 Size: 4704 Fragments arranged by size: 4704 1653 1077 1071 510 366 204 156 85 BsmI GAATG_Cn' Cuts at: 2869 6452 7337 7430 2869 Size: 3583 885 93 5265 Fragments arranged by size: 5265 3583 885 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4886 4958 4886 Size: 72 9754 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2625 3597 3921 4125 4491 4991 5568 Size: 399 835 972 324 204 366 500 577 Cuts at: 5568 5724 6234 7145 7887 7972 9043 1391 Size: 156 510 911 742 85 1071 2174 Fragments arranged by size: 2174 1071 972 911 835 742 577 510 500 399 366 324 204 156 85 BspEI T'CCGG_A Cuts at: 2133 3741 9489 2133 Size: 1608 5748 2470 Fragments arranged by size: 5748 2470 1608 BspHI T'CATG_A Cuts at: 2091 3939 4036 5409 7715 9445 2091 Size: 1848 97 1373 2306 1730 2472 Fragments arranged by size: 2472 2306 1848 1730 1373 97 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3651 5948 7181 844 Size: 2807 2297 1233 3489 Fragments arranged by size: 3489 2807 2297 1233 BsrBI CCG'CTC Cuts at: 1394 1823 5827 5850 7713 1394 Size: 429 4004 23 1863 3507 Fragments arranged by size: 4004 3507 1863 429 23 BsrDI GCAATG_nn' Cuts at: 65 1248 2040 2643 4503 5328 5600 6828 8321 Size: 1183 792 603 1860 825 272 1228 1493 Cuts at: 8321 8495 65 Size: 174 1396 Fragments arranged by size: 1860 1493 1396 1228 1183 825 792 603 272 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3448 4293 4563 5272 8470 8657 1700 Size: 110 1638 845 270 709 3198 187 2869 Fragments arranged by size: 3198 2869 1638 845 709 270 187 110 BsrGI T'GTAC_A Cuts at: 96 3576 96 Size: 3480 6346 BssHII G'CGCG_C Cuts at: 1925 6904 1925 Size: 4979 4847 BssSI C'ACGA_G Cuts at: 5507 7886 8898 5507 Size: 2379 1012 6435 Fragments arranged by size: 6435 2379 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4020 6152 6347 794 Size: 3226 2132 195 4273 Fragments arranged by size: 4273 3226 2132 195 BstBI TT'CG_AA Cuts at: 2932 8630 2932 Size: 5698 4128 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9826 BstXI CCAn_nnnn'nTGG Cuts at: 2016 2294 2444 4003 5803 6163 2016 Size: 278 150 1559 1800 360 5679 Fragments arranged by size: 5679 1800 1559 360 278 150 BstYI r'GATC_y Cuts at: 1972 3423 3455 6413 7156 7523 7908 7925 9377 Size: 1451 32 2958 743 367 385 17 1452 Cuts at: 9377 9463 9475 9821 1972 Size: 86 12 346 1977 Fragments arranged by size: 2958 1977 1452 1451 743 385 367 346 86 32 17 12 BtgI C'CryG_G Cuts at: 513 1065 2015 2563 3990 4029 4221 5748 5796 Size: 552 950 548 1427 39 192 1527 48 Cuts at: 5796 6402 513 Size: 606 3937 Fragments arranged by size: 3937 1527 1427 950 606 552 548 192 48 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2715 3394 4231 4828 487 Size: 683 56 1489 679 837 597 5485 Fragments arranged by size: 5485 1489 837 683 679 597 56 BtsI GCAGTG_nn' Cuts at: 7342 8136 8156 7342 Size: 794 20 9012 Fragments arranged by size: 9012 794 20 ClaI AT'CG_AT Cuts at: 1554 5366 1554 Size: 3812 6014 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9791 DraI TTT'AAA Cuts at: 6076 7130 6076 Size: 1054 8772 DraIII CAC_nnn'GTG Cuts at: 6666 6666 Size: 9826 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4913 6212 8833 809 Size: 507 3597 1299 2621 1802 Fragments arranged by size: 3597 2621 1802 1299 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2446 2813 3446 Size: 54 1425 50 275 18 616 367 633 Cuts at: 3446 3993 4105 4158 4697 5124 5274 5793 6165 Size: 547 112 53 539 427 150 519 372 Cuts at: 6165 7273 8162 9791 8 Size: 1108 889 1629 43 Fragments arranged by size: 1629 1425 1108 889 633 616 547 539 519 427 372 367 275 150 112 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7273 1537 Size: 275 5461 4090 Fragments arranged by size: 5461 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2794 3767 3852 4470 4536 4667 5552 5583 Size: 1441 973 85 618 66 131 885 31 Cuts at: 5583 6537 6953 7756 1353 Size: 954 416 803 3423 Fragments arranged by size: 3423 1441 973 954 885 803 618 416 131 85 66 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3165 8413 8787 8933 387 Size: 1064 138 1576 5248 374 146 1280 Fragments arranged by size: 5248 1576 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3919 4123 4489 5566 6232 3919 Size: 204 366 1077 666 7513 Fragments arranged by size: 7513 1077 666 366 204 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2306 2573 2667 2685 2945 3482 Size: 393 566 108 267 94 18 260 537 Cuts at: 3482 3544 4205 4213 4510 4882 5440 5596 5652 Size: 62 661 8 297 372 558 156 56 Cuts at: 5652 5720 5726 5825 5843 6288 6408 6732 7889 Size: 68 6 99 18 445 120 324 1157 Cuts at: 7889 8486 9273 9541 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1157 787 661 597 566 558 537 445 393 372 324 297 268 267 260 156 120 108 99 94 68 62 56 18 18 8 6 EcoO109I rG'GnC_Cy Cuts at: 2569 2788 3023 3594 4000 5856 6084 2569 Size: 219 235 571 406 1856 228 6311 Fragments arranged by size: 6311 1856 571 406 235 228 219 EcoRI G'AATT_C Cuts at: 4338 4595 7135 7513 4338 Size: 257 2540 378 6651 Fragments arranged by size: 6651 2540 378 257 EcoRV GAT'ATC Cuts at: 5370 5370 Size: 9826 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2626 5108 5152 6033 2626 Size: 2482 44 881 6419 Fragments arranged by size: 6419 2482 881 44 FspI TGC'GCA Cuts at: 4307 8332 4307 Size: 4025 5801 HaeII r_GCGC'y Cuts at: 1678 3739 5812 8973 1678 Size: 2061 2073 3161 2531 Fragments arranged by size: 3161 2531 2073 2061 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2211 2243 2351 2383 2705 Size: 32 135 32 639 32 108 32 322 Cuts at: 2705 2737 2793 2825 3393 3425 4864 4896 5096 Size: 32 56 32 568 32 1439 32 200 Cuts at: 5096 5128 5176 5208 5250 5282 5672 5704 5957 Size: 32 48 32 42 32 390 32 253 Cuts at: 5957 5989 6383 6398 6415 6430 7052 7084 8467 Size: 32 394 15 17 15 622 32 1383 Cuts at: 8467 8499 8541 8573 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1439 1383 639 622 568 394 390 322 253 200 135 108 56 48 42 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 2674 3406 4063 5320 5710 7170 Size: 988 281 736 732 657 1257 390 1460 Cuts at: 7170 7416 669 Size: 246 3079 Fragments arranged by size: 3079 1460 1257 988 736 732 657 390 281 246 HindIII A'AGCT_T Cuts at: 748 2546 6292 7105 748 Size: 1798 3746 813 3469 Fragments arranged by size: 3746 3469 1798 813 HpaI GTT'AAC Cuts at: 2674 7416 2674 Size: 4742 5084 KpnI G_GTAC'C Cuts at: 7151 7151 Size: 9826 MfeI C'AATT_G Cuts at: 6687 7425 6687 Size: 738 9088 MluI A'CGCG_T Cuts at: 9702 9702 Size: 9826 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2478 2514 3456 4846 5397 5862 6011 6042 Size: 1173 36 942 1390 551 465 149 31 Cuts at: 6042 6900 8940 9124 1305 Size: 858 2040 184 2007 Fragments arranged by size: 2040 2007 1390 1173 942 858 551 465 184 149 36 31 MscI TGG'CCA Cuts at: 10 64 1489 1832 2448 3995 4107 4160 5126 Size: 54 1425 343 616 1547 112 53 966 Cuts at: 5126 5795 6167 10 Size: 669 372 3669 Fragments arranged by size: 3669 1547 1425 966 669 616 372 343 112 54 53 MslI CAynn'nnrTG Cuts at: 518 1166 2468 3248 4280 4286 4496 5045 5468 Size: 648 1302 780 1032 6 210 549 423 Cuts at: 5468 5690 6196 6401 7784 8143 8302 518 Size: 222 506 205 1383 359 159 2042 Fragments arranged by size: 2042 1383 1302 1032 780 648 549 506 423 359 222 210 205 159 6 MspA1I CmG'CkG Cuts at: 1606 3181 4111 4156 5239 5770 6994 7242 7919 Size: 1575 930 45 1083 531 1224 248 677 Cuts at: 7919 9067 9312 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1575 1224 1148 1083 930 677 531 248 245 45 NaeI GCC'GGC Cuts at: 4295 5274 4295 Size: 979 8847 NcoI C'CATG_G Cuts at: 513 1065 2563 3990 4029 6402 513 Size: 552 1498 1427 39 2373 3937 Fragments arranged by size: 3937 2373 1498 1427 552 39 NdeI CA'TA_TG Cuts at: 387 6943 387 Size: 6556 3270 NgoMIV G'CCGG_C Cuts at: 4293 5272 4293 Size: 979 8847 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9826 NotI GC'GGCC_GC Cuts at: 7273 7273 Size: 9826 NsiI A_TGCA'T Cuts at: 2871 5600 2871 Size: 2729 7097 NspI r_CATG'y Cuts at: 1132 1229 2294 2869 3541 4285 4306 4393 5146 Size: 97 1065 575 672 744 21 87 753 Cuts at: 5146 5689 7184 8729 1132 Size: 543 1495 1545 2229 Fragments arranged by size: 2229 1545 1495 1065 753 744 672 575 543 97 87 21 PasI CC'CwG_GG Cuts at: 1348 2763 4741 6459 1348 Size: 1415 1978 1718 4715 Fragments arranged by size: 4715 1978 1718 1415 PciI A'CATG_T Cuts at: 4281 5685 8725 4281 Size: 1404 3040 5382 Fragments arranged by size: 5382 3040 1404 PflMI CCAn_nnn'nTGG Cuts at: 1108 2526 3184 5749 1108 Size: 1418 658 2565 5185 Fragments arranged by size: 5185 2565 1418 658 PfoI T'CCnGG_A Cuts at: 2192 4125 4526 2192 Size: 1933 401 7492 Fragments arranged by size: 7492 1933 401 PmeI GTTT'AAAC Cuts at: 7130 7130 Size: 9826 PmlI CAC'GTG Cuts at: 4206 4257 4206 Size: 51 9775 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3211 3243 7895 7927 9408 9440 3211 Size: 32 4652 32 1481 32 3597 Fragments arranged by size: 4652 3597 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2788 3023 5856 6084 2788 Size: 235 2833 228 6530 Fragments arranged by size: 6530 2833 235 228 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9826 PsiI TTA'TAA Cuts at: 2214 7396 2214 Size: 5182 4644 PspOMI G'GGCC_C Cuts at: 3593 4987 3593 Size: 1394 8432 PspXI vC'TCGA_Gb Cuts at: 5851 5851 Size: 9826 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4922 4954 5123 5155 4922 Size: 32 169 32 9593 Fragments arranged by size: 9593 169 32 32 PstI C_TGCA'G Cuts at: 830 2577 4215 4818 5959 6148 7178 830 Size: 1747 1638 603 1141 189 1030 3478 Fragments arranged by size: 3478 1747 1638 1141 1030 603 189 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9826 PvuII CAG'CTG Cuts at: 3181 4111 4156 5239 5770 3181 Size: 930 45 1083 531 7237 Fragments arranged by size: 7237 1083 930 531 45 SacI G_AGCT'C Cuts at: 3921 4125 4491 5568 6234 3921 Size: 204 366 1077 666 7513 Fragments arranged by size: 7513 1077 666 366 204 SalI G'TCGA_C Cuts at: 1936 3404 7168 1936 Size: 1468 3764 4594 Fragments arranged by size: 4594 3764 1468 SapI GCTCTTCn'nnn_ Cuts at: 4667 5583 6953 4667 Size: 916 1370 7540 Fragments arranged by size: 7540 1370 916 SbfI CC_TGCA'GG Cuts at: 5959 7178 5959 Size: 1219 8607 ScaI AGT'ACT Cuts at: 4823 8074 9786 4823 Size: 3251 1712 4863 Fragments arranged by size: 4863 3251 1712 SfcI C'TryA_G Cuts at: 826 1045 2573 3655 4211 4793 4814 5955 6120 Size: 219 1528 1082 556 582 21 1141 165 Cuts at: 6120 6144 7174 8309 8990 9181 826 Size: 24 1030 1135 681 191 1471 Fragments arranged by size: 1528 1471 1141 1135 1082 1030 681 582 556 219 191 165 24 21 SfiI GGCCn_nnn'nGGCC Cuts at: 4007 5282 4007 Size: 1275 8551 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9826 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9826 SmaI CCC'GGG Cuts at: 1872 7153 1872 Size: 5281 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2600 2730 3820 4322 5567 5851 Size: 197 926 657 130 1090 502 1245 284 Cuts at: 5851 6586 6681 7930 8831 9093 820 Size: 735 95 1249 901 262 1553 Fragments arranged by size: 1553 1249 1245 1090 926 901 735 657 502 284 262 197 130 95 SnaBI TAC'GTA Cuts at: 493 7617 493 Size: 7124 2702 SpeI A'CTAG_T Cuts at: 152 6107 152 Size: 5955 3871 SphI G_CATG'C Cuts at: 1132 2869 3541 4306 7184 1132 Size: 1737 672 765 2878 3774 Fragments arranged by size: 3774 2878 1737 765 672 SspI AAT'ATT Cuts at: 5 52 7750 5 Size: 47 7698 2081 Fragments arranged by size: 7698 2081 47 StuI AGG'CCT Cuts at: 1517 4043 4320 1517 Size: 2526 277 7023 Fragments arranged by size: 7023 2526 277 StyI C'CwwG_G Cuts at: 513 1065 2437 2520 2563 3990 3996 4029 6162 Size: 552 1372 83 43 1427 6 33 2133 Cuts at: 6162 6274 6402 7265 513 Size: 112 128 863 3074 Fragments arranged by size: 3074 2133 1427 1372 863 552 128 112 83 43 33 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2972 4624 4721 6016 6367 6449 7853 Size: 1255 1392 1652 97 1295 351 82 1404 Cuts at: 7853 7870 8023 8208 8653 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1652 1498 1404 1392 1295 1255 445 351 185 153 97 82 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3576 4821 5031 Size: 274 80 33 51 1443 1599 1245 210 Cuts at: 5031 5575 8072 9784 96 Size: 544 2497 1712 138 Fragments arranged by size: 2497 1712 1599 1443 1245 544 274 210 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2907 2939 3015 3047 6714 6746 9786 9818 2907 Size: 32 76 32 3667 32 3040 32 2915 Fragments arranged by size: 3667 3040 2915 76 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2827 5860 5971 1316 Size: 1511 3033 111 5171 Fragments arranged by size: 5171 3033 1511 111 XbaI T'CTAG_A Cuts at: 6538 7162 6538 Size: 624 9202 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4003 4003 Size: 9826 XhoI C'TCGA_G Cuts at: 1943 2730 5851 1943 Size: 787 3121 5918 Fragments arranged by size: 5918 3121 787 XmaI C'CCGG_G Cuts at: 1870 7151 1870 Size: 5281 4545 XmnI GAAnn'nnTTC Cuts at: 3203 5135 5546 5927 6013 6962 7955 3203 Size: 1932 411 381 86 949 993 5074 Fragments arranged by size: 5074 1932 993 949 411 381 86 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7634 276 Size: 53 83 186 936 6100 2468 Fragments arranged by size: 6100 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbeI BbvCI BplI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NarI NruI PacI RsrII SacII SanDI SexAI SfoI SrfI SwaI