Restriction map for
pFN21AE5838
(Linear) (Six-base) MAPSORT of: KIEE5838.seq Check: 2228 from: 1 to: 5121
KIEE5838.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3943 5121
Size: 3943 1178
AccI GT'mk_AC
Cuts at: 0 1400 5121
Size: 1400 3721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 301 2200 2877 3435 3591 3647 4403
Size: 193 108 1899 677 558 156 56 756
Cuts at: 4403 5121
Size: 718
Fragments arranged by size:
1899 756 718 677 558 193 156 108
56
AfeI AGC'GCT
Cuts at: 0 1732 5121
Size: 1732 3389
AflIII A'CryG_T
Cuts at: 0 2249 2276 3680 5121
Size: 2249 27 1404 1441
Fragments arranged by size:
2249 1441 1404 27
AgeI A'CCGG_T
Cuts at: 0 2558 5121
Size: 2558 2563
AhdI GACnn_n'nnGTC
Cuts at: 0 360 5061 5121
Size: 360 4701 60
Fragments arranged by size:
4701 360 60
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2278 5121
Size: 2278 2843
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3610 3642 5121
Size: 3610 32 1479
Fragments arranged by size:
3610 1479 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1230 2076 2265 3480 4147 5121
Size: 1230 846 189 1215 667 974
Fragments arranged by size:
1230 1215 974 846 667 189
ApaI G_GGCC'C
Cuts at: 0 1592 2986 5121
Size: 1592 1394 2135
Fragments arranged by size:
2135 1592 1394
ApoI r'AATT_y
Cuts at: 0 72 2333 2590 5121
Size: 72 2261 257 2531
Fragments arranged by size:
2531 2261 257 72
AvaI C'yCGr_G
Cuts at: 0 725 3209 3846 5121
Size: 725 2484 637 1275
Fragments arranged by size:
2484 1275 725 637
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4286 4319 5121
Size: 4286 33 802
Fragments arranged by size:
4286 802 33
BanI G'GyrC_C
Cuts at: 0 4608 5121
Size: 4608 513
BanII G_rGCy'C
Cuts at: 0 620 1592 1916 2120 2486 2986 3563 4229
Size: 620 972 324 204 366 500 577 666
Cuts at: 4229 5121
Size: 892
Fragments arranged by size:
972 892 666 620 577 500 366 324
204
BbsI GAAGACnn'nnnn_
Cuts at: 0 936 1201 3776 4188 5121
Size: 936 265 2575 412 933
Fragments arranged by size:
2575 936 933 412 265
BciVI GTATCCnnnnn_n'
Cuts at: 0 2638 3644 5121
Size: 2638 1006 1477
Fragments arranged by size:
2638 1477 1006
BclI T'GATC_A
Cuts at: 0 3443 5121
Size: 3443 1678
BfrBI ATG'CAT
Cuts at: 0 864 3593 5121
Size: 864 2729 1528
Fragments arranged by size:
2729 1528 864
BglI GCCn_nnn'nGGC
Cuts at: 0 2002 3277 5121
Size: 2002 1275 1844
Fragments arranged by size:
2002 1844 1275
BglII A'GATC_T
Cuts at: 0 1418 4408 5121
Size: 1418 2990 713
Fragments arranged by size:
2990 1418 713
BlpI GC'TnA_GC
Cuts at: 0 1223 1377 5121
Size: 1223 154 3744
Fragments arranged by size:
3744 1223 154
Bme1580I G_kGCm'C
Cuts at: 0 1592 2986 5121
Size: 1592 1394 2135
Fragments arranged by size:
2135 1592 1394
BmrI ACTGGGnnnn_n'
Cuts at: 0 296 5121
Size: 296 4825
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 662 680 940 1477 1539 2208 2505
Size: 568 94 18 260 537 62 669 297
Cuts at: 2505 3715 3721 3820 3838 4283 4727 5121
Size: 1210 6 99 18 445 444 394
Fragments arranged by size:
1210 669 568 537 445 444 394 297
260 99 94 62 18 18 6
Bpu10I CC'TnA_GC
Cuts at: 0 41 4084 5121
Size: 41 4043 1037
Fragments arranged by size:
4043 1037 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1836 2302 3547 4566 4661 5121
Size: 580 1256 466 1245 1019 95 460
Fragments arranged by size:
1256 1245 1019 580 466 460 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 3199 3955 5121
Size: 3199 756 1166
Fragments arranged by size:
3199 1166 756
BsaAI yAC'GTr
Cuts at: 0 2201 2252 5121
Size: 2201 51 2869
Fragments arranged by size:
2869 2201 51
BsaBI GATnn'nnATC
Cuts at: 0 1669 2716 5121
Size: 1669 1047 2405
Fragments arranged by size:
2405 1669 1047
BsaHI Gr'CG_yC
Cuts at: 0 270 3872 5121
Size: 270 3602 1249
Fragments arranged by size:
3602 1249 270
BsaWI w'CCGG_w
Cuts at: 0 128 1736 2558 5121
Size: 128 1608 822 2563
Fragments arranged by size:
2563 1608 822 128
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 479 509 902 932 3667 3697 3952 3982
Size: 479 30 393 30 2735 30 255 30
Cuts at: 3982 4378 4408 4709 4739 5121
Size: 396 30 301 30 382
Fragments arranged by size:
2735 479 396 393 382 301 255 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 95 487 1993 3115 3310 3973 4807 4936
Size: 95 392 1506 1122 195 663 834 129
Cuts at: 4936 5085 5121
Size: 149 36
Fragments arranged by size:
1506 1122 834 663 392 195 149 129
95 36
BseYI C'CCAG_C
Cuts at: 0 235 1791 4275 4777 5121
Size: 235 1556 2484 502 344
Fragments arranged by size:
2484 1556 502 344 235
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2274 3849 5121
Size: 2274 1575 1272
Fragments arranged by size:
2274 1575 1272
BsiHKAI G_wGCw'C
Cuts at: 0 1916 2120 2486 3563 3719 4229 5121
Size: 1916 204 366 1077 156 510 892
Fragments arranged by size:
1916 1077 892 510 366 204 156
BsmI GAATG_Cn'
Cuts at: 0 864 4447 5121
Size: 864 3583 674
Fragments arranged by size:
3583 864 674
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2881 2953 5121
Size: 2881 72 2168
Fragments arranged by size:
2881 2168 72
Bsp1286I G_dGCh'C
Cuts at: 0 620 1592 1916 2120 2486 2986 3563 3719
Size: 620 972 324 204 366 500 577 156
Cuts at: 3719 4229 5121
Size: 510 892
Fragments arranged by size:
972 892 620 577 510 500 366 324
204 156
BspEI T'CCGG_A
Cuts at: 0 128 1736 5121
Size: 128 1608 3385
Fragments arranged by size:
3385 1608 128
BspHI T'CATG_A
Cuts at: 0 86 1934 2031 3404 5121
Size: 86 1848 97 1373 1717
Fragments arranged by size:
1848 1717 1373 97 86
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1646 3943 5121
Size: 1646 2297 1178
Fragments arranged by size:
2297 1646 1178
BsrBI CCG'CTC
Cuts at: 0 3822 3845 5121
Size: 3822 23 1276
Fragments arranged by size:
3822 1276 23
BsrDI GCAATG_nn'
Cuts at: 0 35 638 2498 3323 3595 4823 5121
Size: 35 603 1860 825 272 1228 298
Fragments arranged by size:
1860 1228 825 603 298 272 35
BsrFI r'CCGG_y
Cuts at: 0 1443 2288 2558 3267 5121
Size: 1443 845 270 709 1854
Fragments arranged by size:
1854 1443 845 709 270
BsrGI T'GTAC_A
Cuts at: 0 1571 5121
Size: 1571 3550
BssHII G'CGCG_C
Cuts at: 0 4899 5121
Size: 4899 222
BssSI C'ACGA_G
Cuts at: 0 3502 5121
Size: 3502 1619
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2015 4147 4342 5121
Size: 2015 2132 195 779
Fragments arranged by size:
2132 2015 779 195
BstBI TT'CG_AA
Cuts at: 0 927 5121
Size: 927 4194
BstXI CCAn_nnnn'nTGG
Cuts at: 0 11 289 439 1998 3798 4158 5121
Size: 11 278 150 1559 1800 360 963
Fragments arranged by size:
1800 1559 963 360 278 150 11
BstYI r'GATC_y
Cuts at: 0 1418 1450 4408 5121
Size: 1418 32 2958 713
Fragments arranged by size:
2958 1418 713 32
BtgI C'CryG_G
Cuts at: 0 10 558 1985 2024 2216 3743 3791 4397
Size: 10 548 1427 39 192 1527 48 606
Cuts at: 4397 5121
Size: 724
Fragments arranged by size:
1527 1427 724 606 548 192 48 39
10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 710 1389 2226 2823 5121
Size: 710 679 837 597 2298
Fragments arranged by size:
2298 837 710 679 597
ClaI AT'CG_AT
Cuts at: 0 3361 5121
Size: 3361 1760
DraI TTT'AAA
Cuts at: 0 4071 5121
Size: 4071 1050
DraIII CAC_nnn'GTG
Cuts at: 0 4661 5121
Size: 4661 460
DrdI GACnn_nn'nnGTC
Cuts at: 0 2908 4207 5121
Size: 2908 1299 914
Fragments arranged by size:
2908 1299 914
EaeI y'GGCC_r
Cuts at: 0 441 808 1441 1988 2100 2153 2692 3119
Size: 441 367 633 547 112 53 539 427
Cuts at: 3119 3269 3788 4160 5121
Size: 150 519 372 961
Fragments arranged by size:
961 633 547 539 519 441 427 372
367 150 112 53
EarI CTCTTCn'nnn_
Cuts at: 0 789 1762 1847 2465 2531 2662 3547 3578
Size: 789 973 85 618 66 131 885 31
Cuts at: 3578 4532 4948 5121
Size: 954 416 173
Fragments arranged by size:
973 954 885 789 618 416 173 131
85 66 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1160 5121
Size: 1160 3961
EcoICRI GAG'CTC
Cuts at: 0 1914 2118 2484 3561 4227 5121
Size: 1914 204 366 1077 666 894
Fragments arranged by size:
1914 1077 894 666 366 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 301 568 662 680 940 1477 1539
Size: 193 108 267 94 18 260 537 62
Cuts at: 1539 2200 2208 2505 2877 3435 3591 3647 3715
Size: 661 8 297 372 558 156 56 68
Cuts at: 3715 3721 3820 3838 4283 4403 4727 5121
Size: 6 99 18 445 120 324 394
Fragments arranged by size:
661 558 537 445 394 372 324 297
267 260 193 156 120 108 99 94
68 62 56 18 18 8 6
EcoO109I rG'GnC_Cy
Cuts at: 0 564 783 1018 1589 1995 3851 4079 5121
Size: 564 219 235 571 406 1856 228 1042
Fragments arranged by size:
1856 1042 571 564 406 235 228 219
EcoRI G'AATT_C
Cuts at: 0 2333 2590 5121
Size: 2333 257 2531
Fragments arranged by size:
2531 2333 257
EcoRV GAT'ATC
Cuts at: 0 3365 5121
Size: 3365 1756
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 621 3103 3147 4028 5121
Size: 621 2482 44 881 1093
Fragments arranged by size:
2482 1093 881 621 44
FspI TGC'GCA
Cuts at: 0 2302 5121
Size: 2302 2819
HaeII r_GCGC'y
Cuts at: 0 1734 3807 5121
Size: 1734 2073 1314
Fragments arranged by size:
2073 1734 1314
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 206 238 346 378 700 732 788 820
Size: 206 32 108 32 322 32 56 32
Cuts at: 820 1388 1420 2859 2891 3091 3123 3171 3203
Size: 568 32 1439 32 200 32 48 32
Cuts at: 3203 3245 3277 3667 3699 3952 3984 4378 4393
Size: 42 32 390 32 253 32 394 15
Cuts at: 4393 4410 4425 5047 5079 5121
Size: 17 15 622 32 42
Fragments arranged by size:
1439 622 568 394 390 322 253 206
200 108 56 48 42 42 32 32
32 32 32 32 32 32 32 32
32 32 17 15 15
HincII GTy'rAC
Cuts at: 0 669 1401 2058 3315 3705 5121
Size: 669 732 657 1257 390 1416
Fragments arranged by size:
1416 1257 732 669 657 390
HindIII A'AGCT_T
Cuts at: 0 541 4287 5100 5121
Size: 541 3746 813 21
Fragments arranged by size:
3746 813 541 21
HpaI GTT'AAC
Cuts at: 0 669 5121
Size: 669 4452
MfeI C'AATT_G
Cuts at: 0 4682 5121
Size: 4682 439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 509 1451 2841 3392 3857 4006 4037
Size: 473 36 942 1390 551 465 149 31
Cuts at: 4037 4895 5121
Size: 858 226
Fragments arranged by size:
1390 942 858 551 473 465 226 149
36 31
MscI TGG'CCA
Cuts at: 0 443 1990 2102 2155 3121 3790 4162 5121
Size: 443 1547 112 53 966 669 372 959
Fragments arranged by size:
1547 966 959 669 443 372 112 53
MslI CAynn'nnrTG
Cuts at: 0 463 1243 2275 2281 2491 3040 3463 3685
Size: 463 780 1032 6 210 549 423 222
Cuts at: 3685 4191 4396 5121
Size: 506 205 725
Fragments arranged by size:
1032 780 725 549 506 463 423 222
210 205 6
MspA1I CmG'CkG
Cuts at: 0 1176 2106 2151 3234 3765 4989 5121
Size: 1176 930 45 1083 531 1224 132
Fragments arranged by size:
1224 1176 1083 930 531 132 45
NaeI GCC'GGC
Cuts at: 0 2290 3269 5121
Size: 2290 979 1852
Fragments arranged by size:
2290 1852 979
NcoI C'CATG_G
Cuts at: 0 558 1985 2024 4397 5121
Size: 558 1427 39 2373 724
Fragments arranged by size:
2373 1427 724 558 39
NdeI CA'TA_TG
Cuts at: 0 4938 5121
Size: 4938 183
NgoMIV G'CCGG_C
Cuts at: 0 2288 3267 5121
Size: 2288 979 1854
Fragments arranged by size:
2288 1854 979
NsiI A_TGCA'T
Cuts at: 0 866 3595 5121
Size: 866 2729 1526
Fragments arranged by size:
2729 1526 866
NspI r_CATG'y
Cuts at: 0 289 864 1536 2280 2301 2388 3141 3684
Size: 289 575 672 744 21 87 753 543
Cuts at: 3684 5121
Size: 1437
Fragments arranged by size:
1437 753 744 672 575 543 289 87
21
PasI CC'CwG_GG
Cuts at: 0 758 2736 4454 5121
Size: 758 1978 1718 667
Fragments arranged by size:
1978 1718 758 667
PciI A'CATG_T
Cuts at: 0 2276 3680 5121
Size: 2276 1404 1441
Fragments arranged by size:
2276 1441 1404
PflMI CCAn_nnn'nTGG
Cuts at: 0 521 1179 3744 5121
Size: 521 658 2565 1377
Fragments arranged by size:
2565 1377 658 521
PfoI T'CCnGG_A
Cuts at: 0 187 2120 2521 5121
Size: 187 1933 401 2600
Fragments arranged by size:
2600 1933 401 187
PmlI CAC'GTG
Cuts at: 0 2201 2252 5121
Size: 2201 51 2869
Fragments arranged by size:
2869 2201 51
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1206 1238 5121
Size: 1206 32 3883
Fragments arranged by size:
3883 1206 32
PpuMI rG'GwC_Cy
Cuts at: 0 783 1018 3851 4079 5121
Size: 783 235 2833 228 1042
Fragments arranged by size:
2833 1042 783 235 228
PsiI TTA'TAA
Cuts at: 0 209 5121
Size: 209 4912
PspOMI G'GGCC_C
Cuts at: 0 1588 2982 5121
Size: 1588 1394 2139
Fragments arranged by size:
2139 1588 1394
PspXI vC'TCGA_Gb
Cuts at: 0 3846 5121
Size: 3846 1275
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2917 2949 3118 3150 5121
Size: 2917 32 169 32 1971
Fragments arranged by size:
2917 1971 169 32 32
PstI C_TGCA'G
Cuts at: 0 572 2210 2813 3954 4143 5121
Size: 572 1638 603 1141 189 978
Fragments arranged by size:
1638 1141 978 603 572 189
PvuII CAG'CTG
Cuts at: 0 1176 2106 2151 3234 3765 5121
Size: 1176 930 45 1083 531 1356
Fragments arranged by size:
1356 1176 1083 930 531 45
SacI G_AGCT'C
Cuts at: 0 1916 2120 2486 3563 4229 5121
Size: 1916 204 366 1077 666 892
Fragments arranged by size:
1916 1077 892 666 366 204
SalI G'TCGA_C
Cuts at: 0 1399 5121
Size: 1399 3722
SapI GCTCTTCn'nnn_
Cuts at: 0 2662 3578 4948 5121
Size: 2662 916 1370 173
Fragments arranged by size:
2662 1370 916 173
SbfI CC_TGCA'GG
Cuts at: 0 3954 5121
Size: 3954 1167
ScaI AGT'ACT
Cuts at: 0 2818 5121
Size: 2818 2303
SfcI C'TryA_G
Cuts at: 0 568 1650 2206 2788 2809 3950 4115 4139
Size: 568 1082 556 582 21 1141 165 24
Cuts at: 4139 5121
Size: 982
Fragments arranged by size:
1141 1082 982 582 568 556 165 24
21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2002 3277 5121
Size: 2002 1275 1844
Fragments arranged by size:
2002 1844 1275
SmlI C'TyrA_G
Cuts at: 0 595 725 1815 2317 3562 3846 4581 4676
Size: 595 130 1090 502 1245 284 735 95
Cuts at: 4676 5121
Size: 445
Fragments arranged by size:
1245 1090 735 595 502 445 284 130
95
SpeI A'CTAG_T
Cuts at: 0 4102 5121
Size: 4102 1019
SphI G_CATG'C
Cuts at: 0 864 1536 2301 5121
Size: 864 672 765 2820
Fragments arranged by size:
2820 864 765 672
StuI AGG'CCT
Cuts at: 0 2038 2315 5121
Size: 2038 277 2806
Fragments arranged by size:
2806 2038 277
StyI C'CwwG_G
Cuts at: 0 432 515 558 1985 1991 2024 4157 4269
Size: 432 83 43 1427 6 33 2133 112
Cuts at: 4269 4397 5121
Size: 128 724
Fragments arranged by size:
2133 1427 724 432 128 112 83 43
33 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 967 2619 2716 4011 4362 4444 5121
Size: 967 1652 97 1295 351 82 677
Fragments arranged by size:
1652 1295 967 677 351 97 82
TatI w'GTAC_w
Cuts at: 0 1571 2816 3026 3570 5121
Size: 1571 1245 210 544 1551
Fragments arranged by size:
1571 1551 1245 544 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 902 934 1010 1042 4709 4741 5121
Size: 902 32 76 32 3667 32 380
Fragments arranged by size:
3667 902 380 76 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 822 3855 3966 5121
Size: 822 3033 111 1155
Fragments arranged by size:
3033 1155 822 111
XbaI T'CTAG_A
Cuts at: 0 4533 5121
Size: 4533 588
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1998 5121
Size: 1998 3123
XhoI C'TCGA_G
Cuts at: 0 725 3846 5121
Size: 725 3121 1275
Fragments arranged by size:
3121 1275 725
XmnI GAAnn'nnTTC
Cuts at: 0 1198 3130 3541 3922 4008 4957 5121
Size: 1198 1932 411 381 86 949 164
Fragments arranged by size:
1932 1198 949 411 381 164 86
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AhdI AlfI
AloI AlwNI ApaI ApoI AvaI BaeI BanI BanII
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
ClaI DraI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PstI PvuII SacI SalI SapI SbfI ScaI
SfcI SfiI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AleI ApaLI AscI AseI
AsiSI AvrII BamHI BbeI BbvCI BcgI BmgBI BmtI
BplI BsiEI BsiWI BstEII BstZ17I Bsu36I BtsI CspCI
EagI EcoNI FseI FspAI KasI KpnI MluI NarI
NheI NotI NruI PacI PmeI PshAI PvuI RsrII
SacII SanDI SexAI SfoI SgfI SgrAI SmaI SnaBI
SrfI SspI SwaI XmaI ZraI