################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HindIII HinfI HpaI KasI Ksp632I KspI MamI MfeI MluI MroI MseI MslI MspA1I MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 171 GGTACC G^GTACC AcyI 481 GGCGTC G[GA]^CG[CT]C AlwNI 302 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 346 AGCGCT AGC^GCT Asp718I 171 GGTACC G^GTACC AvaII 473 GGTCC G^G[AT]CC BalI 2 TGGCCA TGG^CCA BanI 171 GGTACC G^G[CT][GA]CC BbsI 325 GTCTTC GAAGAC(2/6) BfaI 118 CTAG C^TAG BpmI 583 CTGGAG CTGGAG(16/14) BpuAI 325 GTCTTC GAAGAC(2/6) BsaHI 481 GGCGTC G[GA]^CG[CT]C BsgI 250 CTGCAC GTGCAG(16/14) BsiYI 526 CCTGAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 526 CCTGAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 158 GAGAC GTCTC(1/5) BsmBI 158 GAGACG CGTCTC(1/5) BsmFI 474 GTCCC GGGAC(10/14) Bsp1407I 76 TGTACA T^GTACA BspMI 174 ACCTGC ACCTGC(4/8) BsrDI 512 GCAATG GCAATG(2/0) BsrFI 495 GCCGGC [GA]^CCGG[CT] BsrGI 76 TGTACA T^GTACA CfoI 347 GCGC GCG^C Cfr10I 495 GCCGGC [GA]^CCGG[CT] Cfr13I 473 GGTCC G^G[ACGT]CC DrdI 670 GACAATAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 346 AGCGCT AGC^GCT EcoRI 590 GAATTC G^AATTC HaeII 346 AGCGCT [GA]GCGC^[CT] HhaI 347 GCGC GCG^C Hin1I 481 GGCGTC G[GA]^CG[CT]C HinP1I 347 GCGC G^CGC HincII 667 GTTGAC GT[CT]^[GA]AC HindII 667 GTTGAC GT[CT]^[GA]AC KpnI 171 GGTACC GGTAC^C MaeI 118 CTAG C^TAG MaeIII 289 GTGAC ^GT[ACGT]AC MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA NaeI 495 GCCGGC GCC^GGC NgoMIV 495 GCCGGC G^CCGGC Sau96I 473 GGTCC G^G[ACGT]CC SspBI 76 TGTACA T^GTACA TaqI 156 TCGA T^CGA Tsp45I 289 GTGAC ^GT[GC]AC TthHB8I 156 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 590 GAATTC [GA]^AATT[CT] 623 GAATTT AflIII 81 ACACGT A^C[GA][CT]GT 83 ACGTGT ApoI 590 GAATTC [GA]^AATT[CT] 623 GAATTT AspHI 265 GAGCTC G[AT]GC[AT]^C 742 GAGCTC BanII 265 GAGCTC G[GA]GC[CT]^C 742 GAGCTC BbrPI 82 CACGTG CAC^GTG 253 CACGTG BbvI 222 GCAGC GCAGC(8/12) 301 GCAGC BsaAI 82 CACGTG [CT]AC^GT[GA] 253 CACGTG BseRI 423 CTCCTC GAGGAG(10/8) 262 GAGGAG BsiHKAI 265 GAGCTC G[AT]GC[AT]^C 742 GAGCTC BstYI 362 AGATCC [GA]^GATC[CT] 384 AGATCC EaeI 2 TGGCCA [CT]^GGCC[GA] 429 CGGCCA Fnu4HI 222 GCAGC GC^[ACGT]GC 301 GCAGC HgaI 200 GCGTC GACGC(5/10) 482 GCGTC HphI 165 GGTGA GGTGA(8/7) 462 GGTGA ItaI 222 GCAGC GC^[ACGT]GC 301 GCAGC MflI 362 AGATCC [GA]^GATC[CT] 384 AGATCC PmaCI 82 CACGTG CAC^GTG 253 CACGTG PmlI 82 CACGTG CAC^GTG 253 CACGTG SacI 265 GAGCTC GAGCT^C 742 GAGCTC SmlI 238 CTTGAG C^T[CT][GA]AG 739 CTTGAG TseI 222 GCAGC G^C[AT]GC 301 GCAGC Tsp509I 591 AATT ^AATT 624 AATT TspRI 306 CACTG CA[GC]TG[ACGT][ACGT]^ 352 CAGTG XhoII 362 AGATCC [GA]^GATC[CT] 384 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AfaI AluI AlwI BmyI BsaJI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI Cac8I DdeI DpnII EcoRII FokI HaeIII HapII HpaII MaeII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI