(Circular) (Six-base) MAPSORT of: pFN21AE4041.seq Check: 6378 from: 1 to: 5494 pFN21AE4041 5494 bp With 182 enzymes: SgfI * March 28, 2012 13:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3304 278 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2837 1937 Size: 900 4594 Acc65I G'GTAC_C Cuts at: 2176 2815 2176 Size: 639 4855 AclI AA'CG_TT Cuts at: 1563 3621 3994 1563 Size: 2058 373 3063 Fragments arranged by size: 3063 2058 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3557 4941 5209 1632 Size: 1925 1384 268 1917 Fragments arranged by size: 1925 1917 1384 268 AfeI AGC'GCT Cuts at: 2353 2353 Size: 5494 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5297 AflIII A'CryG_T Cuts at: 1203 2086 2088 4393 5370 1203 Size: 883 2 2305 977 1327 Fragments arranged by size: 2305 1327 977 883 2 AgeI A'CCGG_T Cuts at: 4325 4325 Size: 5494 AhdI GACnn_n'nnGTC Cuts at: 4223 4223 Size: 5494 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5494 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5494 AlwNI CAG_nnn'CTG Cuts at: 2312 3237 4809 2312 Size: 925 1572 2997 Fragments arranged by size: 2997 1572 925 ApaLI G'TGCA_C Cuts at: 3551 4707 3551 Size: 1156 4338 ApoI r'AATT_y Cuts at: 1493 2595 2628 2803 3029 3181 5290 1493 Size: 1102 33 175 226 152 2109 1697 Fragments arranged by size: 2109 1697 1102 226 175 152 33 AseI AT'TA_AT Cuts at: 160 1032 4048 160 Size: 872 3016 1606 Fragments arranged by size: 3016 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 AvaI C'yCGr_G Cuts at: 1870 1943 2819 1870 Size: 73 876 4545 Fragments arranged by size: 4545 876 73 BamHI G'GATC_C Cuts at: 2824 3191 2824 Size: 367 5127 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2176 2815 4270 618 Size: 325 217 627 389 639 1455 1842 Fragments arranged by size: 1842 1455 639 627 389 325 217 BanII G_rGCy'C Cuts at: 1391 2274 2751 2813 1391 Size: 883 477 62 4072 Fragments arranged by size: 4072 883 477 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2323 928 Size: 971 424 4099 Fragments arranged by size: 4099 971 424 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3685 3719 1903 Size: 34 1748 34 3678 Fragments arranged by size: 3678 1748 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3386 4596 3386 Size: 1210 4284 BclI T'GATC_A Cuts at: 1548 1548 Size: 5494 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4105 136 Size: 107 122 71 631 605 2433 1525 Fragments arranged by size: 2433 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5489 5489 Size: 5494 BlpI GC'TnA_GC Cuts at: 2911 2911 Size: 5494 Bme1580I G_kGCm'C Cuts at: 1790 2153 3555 4711 1790 Size: 363 1402 1156 2573 Fragments arranged by size: 2573 1402 1156 363 BmgBI CAC'GTC Cuts at: 1318 2167 1318 Size: 849 4645 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2455 2786 3225 Size: 416 53 83 399 24 1038 331 439 Cuts at: 3225 4183 442 Size: 958 1753 Fragments arranged by size: 1753 1038 958 439 416 399 331 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2609 4154 1239 Size: 1370 1545 2579 Fragments arranged by size: 2579 1545 1370 Bpu10I CC'TnA_GC Cuts at: 2532 2532 Size: 5494 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2264 2765 3619 4484 4782 2264 Size: 501 854 865 298 2976 Fragments arranged by size: 2976 865 854 501 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4157 882 Size: 617 2658 2219 Fragments arranged by size: 2658 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2089 2260 3285 493 Size: 711 885 171 1025 2702 Fragments arranged by size: 2702 1025 885 711 171 BsaBI GATnn'nnATC Cuts at: 5488 5488 Size: 5494 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2487 3301 3683 4318 Size: 53 83 186 936 954 814 382 635 Cuts at: 4318 275 Size: 1451 Fragments arranged by size: 1451 954 936 814 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3927 4325 4599 4746 5157 3927 Size: 398 274 147 411 4264 Fragments arranged by size: 4264 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2711 2741 1178 Size: 30 165 30 1308 30 3931 Fragments arranged by size: 3931 1308 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2282 2419 1186 Size: 1096 137 4261 Fragments arranged by size: 4261 1096 137 BseYI C'CCAG_C Cuts at: 4697 5193 4697 Size: 496 4998 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2240 1163 Size: 573 504 4417 Fragments arranged by size: 4417 573 504 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2944 3705 4733 1540 Size: 275 186 943 761 1028 2301 Fragments arranged by size: 2301 1028 943 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2274 2751 3555 3640 4711 2274 Size: 477 804 85 1071 3057 Fragments arranged by size: 3057 1071 804 477 85 BsmI GAATG_Cn' Cuts at: 3005 3098 3005 Size: 93 5401 BsmBI CGTCTCn'nnnn_ Cuts at: 2157 2157 Size: 5494 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2153 2274 2751 2813 3555 3640 4711 Size: 399 363 121 477 62 742 85 1071 Cuts at: 4711 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 477 399 363 121 85 62 BspEI T'CCGG_A Cuts at: 5157 5157 Size: 5494 BspHI T'CATG_A Cuts at: 3383 5113 3383 Size: 1730 3764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2188 2849 844 Size: 1344 661 3489 Fragments arranged by size: 3489 1344 661 BsrBI CCG'CTC Cuts at: 1394 1823 3381 1394 Size: 429 1558 3507 Fragments arranged by size: 3507 1558 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2524 3989 4163 65 Size: 1183 1276 1465 174 1396 Fragments arranged by size: 1465 1396 1276 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2500 4138 4325 1700 Size: 110 690 1638 187 2869 Fragments arranged by size: 2869 1638 690 187 110 BsrGI T'GTAC_A Cuts at: 96 2081 96 Size: 1985 3509 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5494 BssSI C'ACGA_G Cuts at: 3554 4566 3554 Size: 1012 4482 BstAPI GCAn_nnn'nTGC Cuts at: 794 2254 2312 2573 794 Size: 1460 58 261 3715 Fragments arranged by size: 3715 1460 261 58 BstBI TT'CG_AA Cuts at: 4298 4298 Size: 5494 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5494 BstYI r'GATC_y Cuts at: 1972 2367 2389 2824 3191 3576 3593 5045 5131 Size: 395 22 435 367 385 17 1452 86 Cuts at: 5131 5143 5489 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 435 395 385 367 346 86 22 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4003 Fragments arranged by size: 4003 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2159 487 Size: 683 56 933 3822 Fragments arranged by size: 3822 933 683 56 BtsI GCAGTG_nn' Cuts at: 2310 3010 3804 3824 2310 Size: 700 794 20 3980 Fragments arranged by size: 3980 794 700 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5494 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5459 DraI TTT'AAA Cuts at: 2798 2798 Size: 5494 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2681 4501 809 Size: 507 1365 1820 1802 Fragments arranged by size: 1820 1802 1365 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2434 2941 Size: 54 1425 50 275 18 177 427 507 Cuts at: 2941 3830 5459 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 507 427 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2941 1537 Size: 275 1129 4090 Fragments arranged by size: 4090 1129 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3424 1353 Size: 2071 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4081 4455 4601 387 Size: 1064 138 2492 374 146 1280 Fragments arranged by size: 2492 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2272 2749 2272 Size: 477 5017 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2609 3557 4154 4941 5209 1239 Size: 393 977 948 597 787 268 1524 Fragments arranged by size: 1524 977 948 787 597 393 268 EcoRI G'AATT_C Cuts at: 2595 2803 3181 2595 Size: 208 378 4908 Fragments arranged by size: 4908 378 208 FspI TGC'GCA Cuts at: 4000 4000 Size: 5494 HaeII r_GCGC'y Cuts at: 1678 2355 4641 1678 Size: 677 2286 2531 Fragments arranged by size: 2531 2286 677 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 2374 2406 2456 Size: 32 135 32 470 32 300 32 50 Cuts at: 2456 2488 4135 4167 4209 4241 1373 Size: 32 1647 32 42 32 2626 Fragments arranged by size: 2626 1647 470 300 135 50 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2674 2838 3084 669 Size: 988 281 736 164 246 3079 Fragments arranged by size: 3079 988 736 281 246 164 HindIII A'AGCT_T Cuts at: 748 748 Size: 5494 HpaI GTT'AAC Cuts at: 3084 3084 Size: 5494 KpnI G_GTAC'C Cuts at: 2180 2819 2180 Size: 639 4855 MfeI C'AATT_G Cuts at: 3093 3093 Size: 5494 MluI A'CGCG_T Cuts at: 5370 5370 Size: 5494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4608 4792 1305 Size: 3303 184 2007 Fragments arranged by size: 3303 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3495 Fragments arranged by size: 3495 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3452 3811 3970 518 Size: 648 2286 359 159 2042 Fragments arranged by size: 2286 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2910 3587 4735 4980 1606 Size: 1304 677 1148 245 2120 Fragments arranged by size: 2120 1304 1148 677 245 NaeI GCC'GGC Cuts at: 2502 2502 Size: 5494 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4003 Fragments arranged by size: 4003 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5494 NgoMIV G'CCGG_C Cuts at: 2500 2500 Size: 5494 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5494 NotI GC'GGCC_GC Cuts at: 2941 2941 Size: 5494 NspI r_CATG'y Cuts at: 1132 1229 2852 4397 1132 Size: 97 1623 1545 2229 Fragments arranged by size: 2229 1623 1545 97 PasI CC'CwG_GG Cuts at: 1348 2482 1348 Size: 1134 4360 PciI A'CATG_T Cuts at: 4393 4393 Size: 5494 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5494 PfoI T'CCnGG_A Cuts at: 2634 2634 Size: 5494 PmeI GTTT'AAAC Cuts at: 2798 2798 Size: 5494 PmlI CAC'GTG Cuts at: 2089 2260 2089 Size: 171 5323 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3563 3595 5076 5108 3563 Size: 32 1481 32 3949 Fragments arranged by size: 3949 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5494 PsiI TTA'TAA Cuts at: 3064 3064 Size: 5494 PstI C_TGCA'G Cuts at: 830 2846 830 Size: 2016 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5494 SacI G_AGCT'C Cuts at: 2274 2751 2274 Size: 477 5017 SalI G'TCGA_C Cuts at: 1936 2836 1936 Size: 900 4594 SbfI CC_TGCA'GG Cuts at: 2846 2846 Size: 5494 ScaI AGT'ACT Cuts at: 3742 5454 3742 Size: 1712 3782 SfcI C'TryA_G Cuts at: 826 1045 2842 3977 4658 4849 826 Size: 219 1797 1135 681 191 1471 Fragments arranged by size: 1797 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5494 SmaI CCC'GGG Cuts at: 1872 2821 1872 Size: 949 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2243 2744 3598 4499 4761 820 Size: 197 926 300 501 854 901 262 1553 Fragments arranged by size: 1553 926 901 854 501 300 262 197 SnaBI TAC'GTA Cuts at: 493 3285 493 Size: 2792 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5494 SphI G_CATG'C Cuts at: 1132 2852 1132 Size: 1720 3774 SspI AAT'ATT Cuts at: 5 52 3418 5 Size: 47 3366 2081 Fragments arranged by size: 3366 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5494 StyI C'CwwG_G Cuts at: 513 1065 2004 2933 513 Size: 552 939 929 3074 Fragments arranged by size: 3074 939 929 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2597 3521 3538 3691 3876 4321 325 Size: 1255 1017 924 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1017 924 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2081 3740 5452 Size: 274 80 33 51 1443 104 1659 1712 Cuts at: 5452 96 Size: 138 Fragments arranged by size: 1712 1659 1443 274 138 104 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2711 2743 5454 5486 2711 Size: 32 2711 32 2719 Fragments arranged by size: 2719 2711 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5494 XbaI T'CTAG_A Cuts at: 2830 2830 Size: 5494 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5494 XmaI C'CCGG_G Cuts at: 1870 2819 1870 Size: 949 4545 XmnI GAAnn'nnTTC Cuts at: 3623 3623 Size: 5494 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3302 276 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI