Restriction map for
pFN21AE4041
(Linear) (Six-base) MAPSORT of: KIEE4041.seq Check: 7089 from: 1 to: 789
KIEE4041.orf
With 182 enzymes: SgfI *
March 22, 2012 17:14 ..
Acc65I G'GTAC_C
Cuts at: 0 171 789
Size: 171 618
AfeI AGC'GCT
Cuts at: 0 348 789
Size: 348 441
AflIII A'CryG_T
Cuts at: 0 81 83 789
Size: 81 2 706
Fragments arranged by size:
706 81 2
AlwNI CAG_nnn'CTG
Cuts at: 0 307 789
Size: 307 482
ApoI r'AATT_y
Cuts at: 0 590 623 789
Size: 590 33 166
Fragments arranged by size:
590 166 33
BanI G'GyrC_C
Cuts at: 0 171 789
Size: 171 618
BanII G_rGCy'C
Cuts at: 0 269 746 789
Size: 269 477 43
Fragments arranged by size:
477 269 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 318 789
Size: 318 471
Bme1580I G_kGCm'C
Cuts at: 0 148 789
Size: 148 641
BmgBI CAC'GTC
Cuts at: 0 162 789
Size: 162 627
BmrI ACTGGGnnnn_n'
Cuts at: 0 450 789
Size: 450 339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 789
Size: 604 185
Bpu10I CC'TnA_GC
Cuts at: 0 527 789
Size: 527 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 760 789
Size: 259 501 29
Fragments arranged by size:
501 259 29
BsaAI yAC'GTr
Cuts at: 0 84 255 789
Size: 84 171 534
Fragments arranged by size:
534 171 84
BsaHI Gr'CG_yC
Cuts at: 0 482 789
Size: 482 307
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 706 736 789
Size: 706 30 53
Fragments arranged by size:
706 53 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 414 789
Size: 277 137 375
Fragments arranged by size:
375 277 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 789
Size: 235 554
BsiHKAI G_wGCw'C
Cuts at: 0 269 746 789
Size: 269 477 43
Fragments arranged by size:
477 269 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 152 789
Size: 152 637
Bsp1286I G_dGCh'C
Cuts at: 0 148 269 746 789
Size: 148 121 477 43
Fragments arranged by size:
477 148 121 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 183 789
Size: 183 606
BsrDI GCAATG_nn'
Cuts at: 0 519 789
Size: 519 270
BsrFI r'CCGG_y
Cuts at: 0 495 789
Size: 495 294
BsrGI T'GTAC_A
Cuts at: 0 76 789
Size: 76 713
BstAPI GCAn_nnn'nTGC
Cuts at: 0 249 307 568 789
Size: 249 58 261 221
Fragments arranged by size:
261 249 221 58
BstYI r'GATC_y
Cuts at: 0 362 384 789
Size: 362 22 405
Fragments arranged by size:
405 362 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 154 789
Size: 154 635
BtsI GCAGTG_nn'
Cuts at: 0 305 789
Size: 305 484
DrdI GACnn_nn'nnGTC
Cuts at: 0 676 789
Size: 676 113
EaeI y'GGCC_r
Cuts at: 0 2 429 789
Size: 2 427 360
Fragments arranged by size:
427 360 2
EcoICRI GAG'CTC
Cuts at: 0 267 744 789
Size: 267 477 45
Fragments arranged by size:
477 267 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 789
Size: 604 185
EcoRI G'AATT_C
Cuts at: 0 590 789
Size: 590 199
HaeII r_GCGC'y
Cuts at: 0 350 789
Size: 350 439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 369 401 451 483 789
Size: 37 32 300 32 50 32 306
Fragments arranged by size:
306 300 50 37 32 32 32
HincII GTy'rAC
Cuts at: 0 669 789
Size: 669 120
KpnI G_GTAC'C
Cuts at: 0 175 789
Size: 175 614
MscI TGG'CCA
Cuts at: 0 4 789
Size: 4 785
NaeI GCC'GGC
Cuts at: 0 497 789
Size: 497 292
NgoMIV G'CCGG_C
Cuts at: 0 495 789
Size: 495 294
PasI CC'CwG_GG
Cuts at: 0 477 789
Size: 477 312
PfoI T'CCnGG_A
Cuts at: 0 629 789
Size: 629 160
PmlI CAC'GTG
Cuts at: 0 84 255 789
Size: 84 171 534
Fragments arranged by size:
534 171 84
SacI G_AGCT'C
Cuts at: 0 269 746 789
Size: 269 477 43
Fragments arranged by size:
477 269 43
SmlI C'TyrA_G
Cuts at: 0 238 739 789
Size: 238 501 50
Fragments arranged by size:
501 238 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 592 789
Size: 592 197
TatI w'GTAC_w
Cuts at: 0 76 789
Size: 76 713
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 706 738 789
Size: 706 32 51
Fragments arranged by size:
706 51 32
Enzymes that do cut:
Acc65I AfeI AflIII AlwNI ApoI BanI BanII BbsI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI
BsrFI BsrGI BstAPI BstYI BtgZI BtsI DrdI EaeI
EcoICRI Eco57MI EcoRI HaeII Hin4I HincII KpnI MscI
NaeI NgoMIV PasI PfoI PmlI SacI SmlI TaqII
TatI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmtI BplI BsaI
BsaBI BsaWI BseYI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraI DraIII EagI EarI EciI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI MfeI MluI MmeI MslI MspA1I NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI