Restriction map for
pFN21AE6752
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BfrI
BglI BlnI BsaHI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspEI BspHI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Bsu36I Cfr10I
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp509I Tth111I XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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ApaI 748 GGGCCC GGGCC^C
Asp700I 229 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 322 GGCACC G^G[CT][GA]CC
BbrPI 985 CACGTG CAC^GTG
BclI 1098 TGATCA T^GATCA
BglII 176 AGATCT A^GATCT
BsaAI 985 CACGTG [CT]AC^GT[GA]
BsaBI 1710 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1739 TCCGGT [AT]^CCGG[AT]
BsgI 1255 GTGCAG GTGCAG(16/14)
BsmI 340 GCATTC GAATGC(1/-1)
BspDI 1207 ATCGAT AT^CGAT
BsrBI 691 GAGCGG CCGCTC(-3/-3)
BstAPI 699 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 912 GGTGACC G^GT[ACGT]ACC
BstPI 912 GGTGACC G^GT[ACGT]ACC
BstXI 1050 CCACACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1207 ATCGAT AT^CGAT
EcoNI 1245 CCTCACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 912 GGTGACC G^GT[ACGT]ACC
EcoT22I 1599 ATGCAT ATGCA^T
FauI 1551 GCGGG CCCGC(4/6)
FbaI 1098 TGATCA T^GATCA
HaeII 1554 GGCGCT [GA]GCGC^[CT]
HindIII 722 AAGCTT A^AGCTT
MamI 1710 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 245 CCATGG C^CATGG
NsiI 1599 ATGCAT ATGCA^T
PaeR7I 1849 CTCGAG C^TCGAG
PmaCI 985 CACGTG CAC^GTG
PmlI 985 CACGTG CAC^GTG
PpuMI 1143 GGGTCCC [GA]G^G[AT]CC[CT]
SapI 1542 GCTCTTC GCTCTTC(1/4)
SexAI 992 ACCTGGT A^CC[AT]GGT
SmlI 1849 CTCGAG C^T[CT][GA]AG
XbaI 120 TCTAGA T^CTAGA
XhoI 1849 CTCGAG C^TCGAG
XmnI 229 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AvaI 751 CCCGAG C^[CT]CG[GA]G
1849 CTCGAG
BpmI 663 CTGGAG CTGGAG(16/14)
970 CTGGAG
BsaI 34 GAGACC GGTCTC(1/5)
757 GGTCTC
BsoBI 751 CCCGAG C^[CT]CG[GA]G
1849 CTCGAG
BspMI 415 GCAGGT ACCTGC(4/8)
1272 GCAGGT
CfoI 1555 GCGC GCG^C
1833 GCGC
DraII 1143 GGGTCCC [GA]G^G[ACGT]CC[CT]
1301 AGGCCCT
EcoO109I 1143 GGGTCCC [GA]G^G[ACGT]CC[CT]
1301 AGGCCCT
EcoT14I 245 CCATGG C^C[AT][AT]GG
1589 CCAAGG
HhaI 1555 GCGC GCG^C
1833 GCGC
HinP1I 1555 GCGC G^CGC
1833 GCGC
MaeII 986 ACGT A^CGT
1868 ACGT
MseI 362 TTAA T^TAA
1115 TTAA
PflMI 1299 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1509 CCACATGTTGG
PleI 218 GACTC GAGTC(4/5)
542 GACTC
PstI 1567 CTGCAG CTGCA^G
1775 CTGCAG
SacI 902 GAGCTC GAGCT^C
1129 GAGCTC
StyI 245 CCATGG C^C[AT][AT]GG
1589 CCAAGG
TfiI 229 GAATC G^A[AT]TC
801 GATTC
Tru9I 362 TTAA T^TAA
1115 TTAA
Van91I 1299 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1509 CCACATGTTGG
XcmI 245 CCATGGTACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
797 CCAAGATTCTGCTGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaII BalI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI Bpu1102I BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp45I TspRI
TthHB8I XhoII