(Circular) (Six-base) MAPSORT of: pFN21AE6752.seq Check: 9836 from: 1 to: 6580 pFN21AE6752 6580 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2411 3268 2411 Size: 857 5723 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4390 278 Size: 53 83 186 936 2854 2468 Fragments arranged by size: 2854 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3923 1937 Size: 1986 4594 Acc65I G'GTAC_C Cuts at: 3901 3901 Size: 6580 AclI AA'CG_TT Cuts at: 1563 4707 5080 1563 Size: 3144 373 3063 Fragments arranged by size: 3144 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2306 2513 3371 4643 6027 6295 1632 Size: 674 207 858 1272 1384 268 1917 Fragments arranged by size: 1917 1384 1272 858 674 268 207 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6383 AflIII A'CryG_T Cuts at: 1203 2029 2991 3114 3516 5479 6456 1203 Size: 826 962 123 402 1963 977 1327 Fragments arranged by size: 1963 1327 977 962 826 402 123 AgeI A'CCGG_T Cuts at: 5411 5411 Size: 6580 AhdI GACnn_n'nnGTC Cuts at: 5309 5309 Size: 6580 AleI CACnn'nnGTG Cuts at: 1166 3257 1166 Size: 2091 4489 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2190 1184 Size: 1006 5574 AlwNI CAG_nnn'CTG Cuts at: 2141 3016 3310 3646 4323 5895 2141 Size: 875 294 336 677 1572 2826 Fragments arranged by size: 2826 1572 875 677 336 294 ApaI G_GGCC'C Cuts at: 2757 2757 Size: 6580 ApaLI G'TGCA_C Cuts at: 4637 5793 4637 Size: 1156 5424 ApoI r'AATT_y Cuts at: 1493 3889 4115 4267 6376 1493 Size: 2396 226 152 2109 1697 Fragments arranged by size: 2396 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5134 160 Size: 872 4102 1606 Fragments arranged by size: 4102 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 AvaI C'yCGr_G Cuts at: 1870 1943 2756 3854 3905 1870 Size: 73 813 1098 51 4545 Fragments arranged by size: 4545 1098 813 73 51 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3207 3240 3207 Size: 33 6547 BamHI G'GATC_C Cuts at: 3910 4277 3910 Size: 367 6213 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2327 3901 5356 618 Size: 325 217 627 540 1574 1455 1842 Fragments arranged by size: 1842 1574 1455 627 540 325 217 BanII G_rGCy'C Cuts at: 1391 2757 2911 3138 3899 1391 Size: 1366 154 227 761 4072 Fragments arranged by size: 4072 1366 761 227 154 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2596 2670 3217 3296 928 Size: 971 697 74 547 79 4212 Fragments arranged by size: 4212 971 697 547 79 74 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4771 4805 1903 Size: 34 50 34 2750 34 3678 Fragments arranged by size: 3678 2750 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4472 5682 4472 Size: 1210 5370 BclI T'GATC_A Cuts at: 1548 3103 1548 Size: 1555 5025 BfrBI ATG'CAT Cuts at: 3606 3606 Size: 6580 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5191 136 Size: 107 122 71 631 605 3519 1525 Fragments arranged by size: 3519 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2181 6575 2181 Size: 4394 2186 BlpI GC'TnA_GC Cuts at: 3132 3443 3731 3997 3132 Size: 311 288 266 5715 Fragments arranged by size: 5715 311 288 266 Bme1580I G_kGCm'C Cuts at: 1790 2330 2757 4641 5797 1790 Size: 540 427 1884 1156 2573 Fragments arranged by size: 2573 1884 1156 540 427 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6580 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3148 4311 5269 Size: 416 53 83 399 24 1731 1163 958 Cuts at: 5269 442 Size: 1753 Fragments arranged by size: 1753 1731 1163 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6580 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2689 2996 5240 1239 Size: 1450 307 2244 2579 Fragments arranged by size: 2579 2244 1450 307 Bpu10I CC'TnA_GC Cuts at: 3765 3765 Size: 6580 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4705 5570 5868 4705 Size: 865 298 5417 Fragments arranged by size: 5417 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2033 2768 5243 882 Size: 617 534 735 2475 2219 Fragments arranged by size: 2475 2219 735 617 534 BsaAI yAC'GTr Cuts at: 493 1204 2992 4371 493 Size: 711 1788 1379 2702 Fragments arranged by size: 2702 1788 1379 711 BsaBI GATnn'nnATC Cuts at: 3719 6574 3719 Size: 2855 3725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4387 4769 5404 275 Size: 53 83 186 936 2854 382 635 1451 Fragments arranged by size: 2854 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3744 5013 5411 5685 5832 6243 3744 Size: 1269 398 274 147 411 4081 Fragments arranged by size: 4081 1269 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6355 Fragments arranged by size: 6355 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 2764 3608 1186 Size: 1021 557 844 4158 Fragments arranged by size: 4158 1021 844 557 BseYI C'CCAG_C Cuts at: 2264 3639 3821 5783 6279 2264 Size: 1375 182 1962 496 2565 Fragments arranged by size: 2565 1962 1375 496 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3281 1163 Size: 573 1545 4462 Fragments arranged by size: 4462 1545 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4030 4791 5819 1540 Size: 275 186 2029 761 1028 2301 Fragments arranged by size: 2301 2029 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2739 2911 3138 3484 3752 3817 4641 4726 5797 Size: 172 227 346 268 65 824 85 1071 Cuts at: 5797 2739 Size: 3522 Fragments arranged by size: 3522 1071 824 346 268 227 172 85 65 BsmI GAATG_Cn' Cuts at: 2345 4091 4184 2345 Size: 1746 93 4741 Fragments arranged by size: 4741 1746 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2330 2739 2757 2911 3138 3484 3752 Size: 399 540 409 18 154 227 346 268 Cuts at: 3752 3817 3899 4641 4726 5797 1391 Size: 65 82 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 540 409 399 346 268 227 154 85 82 65 18 BspEI T'CCGG_A Cuts at: 6243 6243 Size: 6580 BspHI T'CATG_A Cuts at: 4469 6199 4469 Size: 1730 4850 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2411 3268 3935 844 Size: 1567 857 667 3489 Fragments arranged by size: 3489 1567 857 667 BsrBI CCG'CTC Cuts at: 1394 1823 2698 4467 1394 Size: 429 875 1769 3507 Fragments arranged by size: 3507 1769 875 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2174 2415 2856 3024 5075 5249 65 Size: 1183 926 241 441 168 2051 174 1396 Fragments arranged by size: 2051 1396 1183 926 441 241 174 168 BsrFI r'CCGG_y Cuts at: 1700 1810 5224 5411 1700 Size: 110 3414 187 2869 Fragments arranged by size: 3414 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6580 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6580 BssSI C'ACGA_G Cuts at: 4640 5652 4640 Size: 1012 5568 BstAPI GCAn_nnn'nTGC Cuts at: 794 2710 794 Size: 1916 4664 BstBI TT'CG_AA Cuts at: 5384 5384 Size: 6580 BstEII G'GTnAC_C Cuts at: 1184 2917 1184 Size: 1733 4847 BstXI CCAn_nnnn'nTGG Cuts at: 3062 3062 Size: 6580 BstYI r'GATC_y Cuts at: 1972 2122 2181 3501 3858 3910 4277 4662 4679 Size: 150 59 1320 357 52 367 385 17 Cuts at: 4679 6131 6217 6229 6575 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1320 385 367 357 346 150 86 59 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2250 3085 3324 513 Size: 552 1185 835 239 3769 Fragments arranged by size: 3769 1185 835 552 239 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3706 487 Size: 683 56 2480 3361 Fragments arranged by size: 3361 2480 683 56 BtsI GCAGTG_nn' Cuts at: 3389 3840 4096 4890 4910 3389 Size: 451 256 794 20 5059 Fragments arranged by size: 5059 794 451 256 20 ClaI AT'CG_AT Cuts at: 1554 3213 1554 Size: 1659 4921 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6545 DraI TTT'AAA Cuts at: 3884 3884 Size: 6580 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5587 809 Size: 507 4271 1802 Fragments arranged by size: 4271 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2335 2643 3386 Size: 54 1425 50 275 18 505 308 743 Cuts at: 3386 3564 4027 4916 6545 8 Size: 178 463 889 1629 43 Fragments arranged by size: 1629 1425 889 743 505 463 308 275 178 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4027 1537 Size: 275 2215 4090 Fragments arranged by size: 4090 2215 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2510 2951 3554 4510 1353 Size: 1157 441 603 956 3423 Fragments arranged by size: 3423 1157 956 603 441 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5167 5541 5687 387 Size: 1064 138 3578 374 146 1280 Fragments arranged by size: 3578 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2909 3136 2909 Size: 227 6353 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2306 2513 2689 2996 3371 4643 5240 Size: 393 674 207 176 307 375 1272 597 Cuts at: 5240 6027 6295 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1272 787 674 597 393 375 307 268 207 176 EcoNI CCTnn'n_nnAGG Cuts at: 3254 3254 Size: 6580 EcoO109I rG'GnC_Cy Cuts at: 3149 3307 3149 Size: 158 6422 EcoRI G'AATT_C Cuts at: 3889 4267 3889 Size: 378 6202 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3376 3376 Size: 6580 FspI TGC'GCA Cuts at: 5086 5086 Size: 6580 HaeII r_GCGC'y Cuts at: 1678 3563 5727 1678 Size: 1885 2164 2531 Fragments arranged by size: 2531 2164 1885 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2107 2139 2368 2400 2511 Size: 32 135 32 535 32 229 32 111 Cuts at: 2511 2543 5221 5253 5295 5327 1373 Size: 32 2678 32 42 32 2626 Fragments arranged by size: 2678 2626 535 229 135 111 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3924 4170 669 Size: 988 281 1986 246 3079 Fragments arranged by size: 3079 1986 988 281 246 HindIII A'AGCT_T Cuts at: 748 2727 748 Size: 1979 4601 HpaI GTT'AAC Cuts at: 4170 4170 Size: 6580 KpnI G_GTAC'C Cuts at: 3905 3905 Size: 6580 MfeI C'AATT_G Cuts at: 4179 4179 Size: 6580 MluI A'CGCG_T Cuts at: 6456 6456 Size: 6580 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2707 3501 5694 5878 1305 Size: 1402 794 2193 184 2007 Fragments arranged by size: 2193 2007 1402 794 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2645 3388 3566 10 Size: 54 1425 343 813 743 178 3024 Fragments arranged by size: 3024 1425 813 743 343 178 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2540 2951 3257 4538 4897 5056 Size: 648 1191 183 411 306 1281 359 159 Cuts at: 5056 518 Size: 2042 Fragments arranged by size: 2042 1281 1191 648 411 359 306 183 159 MspA1I CmG'CkG Cuts at: 1606 2084 2374 2600 2769 3577 3996 4673 5821 Size: 478 290 226 169 808 419 677 1148 Cuts at: 5821 6066 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 808 677 478 419 290 245 226 169 NcoI C'CATG_G Cuts at: 513 1065 2250 513 Size: 552 1185 4843 Fragments arranged by size: 4843 1185 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6580 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6580 NotI GC'GGCC_GC Cuts at: 4027 4027 Size: 6580 NsiI A_TGCA'T Cuts at: 3608 3608 Size: 6580 NspI r_CATG'y Cuts at: 1132 1229 2033 3118 3520 3938 5483 1132 Size: 97 804 1085 402 418 1545 2229 Fragments arranged by size: 2229 1545 1085 804 418 402 97 PasI CC'CwG_GG Cuts at: 1348 3016 1348 Size: 1668 4912 PciI A'CATG_T Cuts at: 2029 3114 3516 5479 2029 Size: 1085 402 1963 3130 Fragments arranged by size: 3130 1963 1085 402 PflMI CCAn_nnn'nTGG Cuts at: 1108 3310 3520 1108 Size: 2202 210 4168 Fragments arranged by size: 4168 2202 210 PfoI T'CCnGG_A Cuts at: 2187 2777 2880 2187 Size: 590 103 5887 Fragments arranged by size: 5887 590 103 PmeI GTTT'AAAC Cuts at: 3884 3884 Size: 6580 PmlI CAC'GTG Cuts at: 2992 2992 Size: 6580 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4649 4681 6162 6194 4649 Size: 32 1481 32 5035 Fragments arranged by size: 5035 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3149 3149 Size: 6580 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6580 PsiI TTA'TAA Cuts at: 4150 4150 Size: 6580 PspOMI G'GGCC_C Cuts at: 2753 2753 Size: 6580 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3699 3731 3699 Size: 32 6548 PstI C_TGCA'G Cuts at: 830 3576 3784 3932 830 Size: 2746 208 148 3478 Fragments arranged by size: 3478 2746 208 148 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6580 PvuII CAG'CTG Cuts at: 2084 2374 2600 2084 Size: 290 226 6064 Fragments arranged by size: 6064 290 226 SacI G_AGCT'C Cuts at: 2911 3138 2911 Size: 227 6353 SalI G'TCGA_C Cuts at: 1936 3922 1936 Size: 1986 4594 SanDI GG'GwC_CC Cuts at: 3149 3149 Size: 6580 SapI GCTCTTCn'nnn_ Cuts at: 3554 3554 Size: 6580 SbfI CC_TGCA'GG Cuts at: 3932 3932 Size: 6580 ScaI AGT'ACT Cuts at: 4828 6540 4828 Size: 1712 4868 SexAI A'CCwGG_T Cuts at: 2997 2997 Size: 6580 SfcI C'TryA_G Cuts at: 826 1045 2227 2923 3267 3572 3780 3928 5063 Size: 219 1182 696 344 305 208 148 1135 Cuts at: 5063 5744 5935 826 Size: 681 191 1471 Fragments arranged by size: 1471 1182 1135 696 681 344 305 219 208 191 148 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6580 SmaI CCC'GGG Cuts at: 1872 3907 1872 Size: 2035 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3854 4684 5585 5847 820 Size: 197 926 1911 830 901 262 1553 Fragments arranged by size: 1911 1553 926 901 830 262 197 SnaBI TAC'GTA Cuts at: 493 4371 493 Size: 3878 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6580 SphI G_CATG'C Cuts at: 1132 3938 1132 Size: 2806 3774 SspI AAT'ATT Cuts at: 5 52 4504 5 Size: 47 4452 2081 Fragments arranged by size: 4452 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6580 StyI C'CwwG_G Cuts at: 513 1065 2250 3594 4019 513 Size: 552 1185 1344 425 3074 Fragments arranged by size: 3074 1344 1185 552 425 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3445 4607 4624 4777 4962 5407 325 Size: 1255 1865 1162 17 153 185 445 1498 Fragments arranged by size: 1865 1498 1255 1162 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3473 4826 6538 Size: 274 80 33 51 1443 1496 1353 1712 Cuts at: 6538 96 Size: 138 Fragments arranged by size: 1712 1496 1443 1353 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2773 2805 6540 6572 2773 Size: 32 3735 32 2781 Fragments arranged by size: 3735 2781 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6580 XbaI T'CTAG_A Cuts at: 2125 3916 2125 Size: 1791 4789 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 2809 2257 Size: 552 6028 XhoI C'TCGA_G Cuts at: 1943 3854 1943 Size: 1911 4669 XmaI C'CCGG_G Cuts at: 1870 3905 1870 Size: 2035 4545 XmnI GAAnn'nnTTC Cuts at: 2238 4709 2238 Size: 2471 4109 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4388 276 Size: 53 83 186 936 2854 2468 Fragments arranged by size: 2854 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI RsrII SacII SfiI SfoI SrfI SwaI