(Linear) (Six-base) MAPSORT of: KIEE6752.seq Check: 849 from: 1 to: 1875 KIEE6752.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 406 1263 1875 Size: 406 857 612 Fragments arranged by size: 857 612 406 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 508 1366 1875 Size: 301 207 858 509 Fragments arranged by size: 858 509 301 207 AflIII A'CryG_T Cuts at: 0 24 986 1109 1511 1875 Size: 24 962 123 402 364 Fragments arranged by size: 962 402 364 123 24 AleI CACnn'nnGTG Cuts at: 0 1252 1875 Size: 1252 623 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 185 1875 Size: 185 1690 AlwNI CAG_nnn'CTG Cuts at: 0 136 1011 1305 1641 1875 Size: 136 875 294 336 234 Fragments arranged by size: 875 336 294 234 136 ApaI G_GGCC'C Cuts at: 0 752 1875 Size: 752 1123 AvaI C'yCGr_G Cuts at: 0 751 1849 1875 Size: 751 1098 26 Fragments arranged by size: 1098 751 26 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1202 1235 1875 Size: 1202 33 640 Fragments arranged by size: 1202 640 33 BanI G'GyrC_C Cuts at: 0 322 1875 Size: 322 1553 BanII G_rGCy'C Cuts at: 0 752 906 1133 1875 Size: 752 154 227 742 Fragments arranged by size: 752 742 227 154 BbsI GAAGACnn'nnnn_ Cuts at: 0 591 665 1212 1291 1875 Size: 591 74 547 79 584 Fragments arranged by size: 591 584 547 79 74 BclI T'GATC_A Cuts at: 0 1098 1875 Size: 1098 777 BfrBI ATG'CAT Cuts at: 0 1601 1875 Size: 1601 274 BglII A'GATC_T Cuts at: 0 176 1875 Size: 176 1699 BlpI GC'TnA_GC Cuts at: 0 1127 1438 1726 1875 Size: 1127 311 288 149 Fragments arranged by size: 1127 311 288 149 Bme1580I G_kGCm'C Cuts at: 0 325 752 1875 Size: 325 427 1123 Fragments arranged by size: 1123 427 325 BmrI ACTGGGnnnn_n' Cuts at: 0 1143 1875 Size: 1143 732 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 684 991 1875 Size: 684 307 884 Fragments arranged by size: 884 684 307 Bpu10I CC'TnA_GC Cuts at: 0 1760 1875 Size: 1760 115 BsaI GGTCTCn'nnnn_ Cuts at: 0 28 763 1875 Size: 28 735 1112 Fragments arranged by size: 1112 735 28 BsaAI yAC'GTr Cuts at: 0 987 1875 Size: 987 888 BsaBI GATnn'nnATC Cuts at: 0 1714 1875 Size: 1714 161 BsaWI w'CCGG_w Cuts at: 0 1739 1875 Size: 1739 136 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 202 759 1603 1875 Size: 202 557 844 272 Fragments arranged by size: 844 557 272 202 BseYI C'CCAG_C Cuts at: 0 259 1634 1816 1875 Size: 259 1375 182 59 Fragments arranged by size: 1375 259 182 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1276 1875 Size: 1276 599 BsiHKAI G_wGCw'C Cuts at: 0 734 906 1133 1479 1747 1812 1875 Size: 734 172 227 346 268 65 63 Fragments arranged by size: 734 346 268 227 172 65 63 BsmI GAATG_Cn' Cuts at: 0 340 1875 Size: 340 1535 Bsp1286I G_dGCh'C Cuts at: 0 325 734 752 906 1133 1479 1747 1812 Size: 325 409 18 154 227 346 268 65 Cuts at: 1812 1875 Size: 63 Fragments arranged by size: 409 346 325 268 227 154 65 63 18 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 406 1263 1875 Size: 406 857 612 Fragments arranged by size: 857 612 406 BsrBI CCG'CTC Cuts at: 0 693 1875 Size: 693 1182 BsrDI GCAATG_nn' Cuts at: 0 169 410 851 1019 1875 Size: 169 241 441 168 856 Fragments arranged by size: 856 441 241 169 168 BstAPI GCAn_nnn'nTGC Cuts at: 0 705 1875 Size: 705 1170 BstEII G'GTnAC_C Cuts at: 0 912 1875 Size: 912 963 BstXI CCAn_nnnn'nTGG Cuts at: 0 1057 1875 Size: 1057 818 BstYI r'GATC_y Cuts at: 0 117 176 1496 1853 1875 Size: 117 59 1320 357 22 Fragments arranged by size: 1320 357 117 59 22 BtgI C'CryG_G Cuts at: 0 245 1080 1319 1875 Size: 245 835 239 556 Fragments arranged by size: 835 556 245 239 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1701 1875 Size: 1701 174 BtsI GCAGTG_nn' Cuts at: 0 1384 1835 1875 Size: 1384 451 40 Fragments arranged by size: 1384 451 40 ClaI AT'CG_AT Cuts at: 0 1208 1875 Size: 1208 667 EaeI y'GGCC_r Cuts at: 0 330 638 1381 1559 1875 Size: 330 308 743 178 316 Fragments arranged by size: 743 330 316 308 178 EarI CTCTTCn'nnn_ Cuts at: 0 505 946 1549 1875 Size: 505 441 603 326 Fragments arranged by size: 603 505 441 326 EcoICRI GAG'CTC Cuts at: 0 904 1131 1875 Size: 904 227 744 Fragments arranged by size: 904 744 227 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 508 684 991 1366 1875 Size: 301 207 176 307 375 509 Fragments arranged by size: 509 375 307 301 207 176 EcoNI CCTnn'n_nnAGG Cuts at: 0 1249 1875 Size: 1249 626 EcoO109I rG'GnC_Cy Cuts at: 0 1144 1302 1875 Size: 1144 158 573 Fragments arranged by size: 1144 573 158 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1371 1875 Size: 1371 504 HaeII r_GCGC'y Cuts at: 0 1558 1875 Size: 1558 317 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 102 134 363 395 506 538 1875 Size: 102 32 229 32 111 32 1337 Fragments arranged by size: 1337 229 111 102 32 32 32 HindIII A'AGCT_T Cuts at: 0 722 1875 Size: 722 1153 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 702 1496 1875 Size: 702 794 379 Fragments arranged by size: 794 702 379 MscI TGG'CCA Cuts at: 0 640 1383 1561 1875 Size: 640 743 178 314 Fragments arranged by size: 743 640 314 178 MslI CAynn'nnrTG Cuts at: 0 352 535 946 1252 1875 Size: 352 183 411 306 623 Fragments arranged by size: 623 411 352 306 183 MspA1I CmG'CkG Cuts at: 0 79 369 595 764 1572 1875 Size: 79 290 226 169 808 303 Fragments arranged by size: 808 303 290 226 169 79 NcoI C'CATG_G Cuts at: 0 245 1875 Size: 245 1630 NsiI A_TGCA'T Cuts at: 0 1603 1875 Size: 1603 272 NspI r_CATG'y Cuts at: 0 28 1113 1515 1875 Size: 28 1085 402 360 Fragments arranged by size: 1085 402 360 28 PasI CC'CwG_GG Cuts at: 0 1011 1875 Size: 1011 864 PciI A'CATG_T Cuts at: 0 24 1109 1511 1875 Size: 24 1085 402 364 Fragments arranged by size: 1085 402 364 24 PflMI CCAn_nnn'nTGG Cuts at: 0 1305 1515 1875 Size: 1305 210 360 Fragments arranged by size: 1305 360 210 PfoI T'CCnGG_A Cuts at: 0 182 772 875 1875 Size: 182 590 103 1000 Fragments arranged by size: 1000 590 182 103 PmlI CAC'GTG Cuts at: 0 987 1875 Size: 987 888 PpuMI rG'GwC_Cy Cuts at: 0 1144 1875 Size: 1144 731 PspOMI G'GGCC_C Cuts at: 0 748 1875 Size: 748 1127 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1694 1726 1875 Size: 1694 32 149 Fragments arranged by size: 1694 149 32 PstI C_TGCA'G Cuts at: 0 1571 1779 1875 Size: 1571 208 96 Fragments arranged by size: 1571 208 96 PvuII CAG'CTG Cuts at: 0 79 369 595 1875 Size: 79 290 226 1280 Fragments arranged by size: 1280 290 226 79 SacI G_AGCT'C Cuts at: 0 906 1133 1875 Size: 906 227 742 Fragments arranged by size: 906 742 227 SanDI GG'GwC_CC Cuts at: 0 1144 1875 Size: 1144 731 SapI GCTCTTCn'nnn_ Cuts at: 0 1549 1875 Size: 1549 326 SexAI A'CCwGG_T Cuts at: 0 992 1875 Size: 992 883 SfcI C'TryA_G Cuts at: 0 222 918 1262 1567 1775 1875 Size: 222 696 344 305 208 100 Fragments arranged by size: 696 344 305 222 208 100 SmlI C'TyrA_G Cuts at: 0 1849 1875 Size: 1849 26 StyI C'CwwG_G Cuts at: 0 245 1589 1875 Size: 245 1344 286 Fragments arranged by size: 1344 286 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1440 1875 Size: 1440 435 TatI w'GTAC_w Cuts at: 0 1468 1875 Size: 1468 407 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 768 800 1875 Size: 768 32 1075 Fragments arranged by size: 1075 768 32 XbaI T'CTAG_A Cuts at: 0 120 1875 Size: 120 1755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 252 804 1875 Size: 252 552 1071 Fragments arranged by size: 1071 552 252 XhoI C'TCGA_G Cuts at: 0 1849 1875 Size: 1849 26 XmnI GAAnn'nnTTC Cuts at: 0 233 1875 Size: 233 1642 Enzymes that do cut: AarI AcuI AflIII AleI AlfI AlwNI ApaI AvaI BaeI BanI BanII BbsI BclI BfrBI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PsrI PstI PvuII SacI SanDI SapI SexAI SfcI SmlI StyI TaqII TatI TstI XbaI XcmI XhoI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbeI BbvCI BcgI BciVI BglI BmgBI BmtI BplI BpuEI BsaHI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PmeI PpiI PshAI PsiI PspXI PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XmaI ZraI