Restriction map for
pFN21AE5733
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# NO CUT ENZYMES ( 100 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbrPI
BciVI BclI BfrI BglII BlnI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI MamI MfeI
MluI MroI MslI MunI NaeI
NdeI NgoMIV NotI NruI NsiI
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SfiI SgrAI
SmlI SnaBI SpeI SphI SspBI
StuI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1375 GACGTC GACGT^C
AccI 1699 GTCTAC GT^[AC][GT]AC
AflIII 221 ACGTGT A^C[GA][CT]GT
AgeI 143 ACCGGT A^CCGGT
Asp700I 1152 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1069 TGGCCA TGG^CCA
BamHI 1748 GGATCC G^GATCC
BbeI 1916 GGCGCC GGCGC^C
BcgI 582 CGATCCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 707 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1860 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1860 GCTGAGC GC^T[ACGT]AGC
BsaAI 1627 TACGTG [CT]AC^GT[GA]
BsgI 736 GTGCAG GTGCAG(16/14)
BsmBI 218 GAGACG CGTCTC(1/5)
BstBI 192 TTCGAA TT^CGAA
BstXI 1588 CCATCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1860 GCTGAGC GC^T[ACGT]AGC
DraI 1179 TTTAAA TTT^AAA
DrdI 1276 GACTACAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1069 TGGCCA [CT]^GGCC[GA]
EcoNI 495 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1916 GGCGCC [GA]GCGC^[CT]
HindIII 1390 AAGCTT A^AGCTT
KasI 1916 GGCGCC G^GCGCC
KspI 213 CCGCGG CCGC^GG
MluNI 1069 TGGCCA TGG^CCA
MscI 1069 TGGCCA TGG^CCA
NarI 1916 GGCGCC GG^CGCC
NheI 469 GCTAGC G^CTAGC
NspI 171 GCATGT [GA]CATG^[CT]
NspV 192 TTCGAA TT^CGAA
PinAI 143 ACCGGT A^CCGGT
PpuMI 299 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 610 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1614 GAGCTC GAGCT^C
SacII 213 CCGCGG CCGC^GG
SapI 1903 GAAGAGC GCTCTTC(1/4)
SbfI 1689 CCTGCAGG CCTGCA^GG
SexAI 1673 ACCTGGT A^CC[AT]GGT
SfaNI 859 GATGC GCATC(5/9)
SfoI 1916 GGCGCC GGC^GCC
SfuI 192 TTCGAA TT^CGAA
Sse8387I 1689 CCTGCAGG CCTGCA^GG
XmnI 1152 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
ApaI 790 GGGCCC GGGCC^C
831 GGGCCC
AspHI 1614 GAGCTC G[AT]GC[AT]^C
1723 GTGCTC
BbvCI 598 GCTGAGG CCTCAGC(-5/-2)
1831 GCTGAGG
BfaI 470 CTAG C^TAG
1036 CTAG
BsaI 1642 GAGACC GGTCTC(1/5)
146 GGTCTC
BsiHKAI 1614 GAGCTC G[AT]GC[AT]^C
1723 GTGCTC
BsmI 682 GAATGC GAATGC(1/-1)
1031 GAATGC
BspMI 1559 ACCTGC ACCTGC(4/8)
1688 ACCTGC
BsrBI 1482 CCGCTC CCGCTC(-3/-3)
1906 GAGCGG
BsrFI 143 ACCGGT [GA]^CCGG[CT]
1913 ACCGGC
BstYI 487 GGATCT [GA]^GATC[CT]
1748 GGATCC
Cfr10I 143 ACCGGT [GA]^CCGG[CT]
1913 ACCGGC
HphI 1680 GGTGA GGTGA(8/7)
1271 TCACC
MaeI 470 CTAG C^TAG
1036 CTAG
MaeIII 805 GTGAC ^GT[ACGT]AC
1630 GTGAC
MflI 487 GGATCT [GA]^GATC[CT]
1748 GGATCC
MseI 1172 TTAA T^TAA
1180 TTAA
SmaI 775 CCCGGG CCC^GGG
1496 CCCGGG
SspI 1325 AATATT AAT^ATT
1881 AATATT
Tru9I 1172 TTAA T^TAA
1180 TTAA
Tsp45I 805 GTGAC ^GT[GC]AC
1630 GTGAC
Tsp509I 424 AATT ^AATT
940 AATT
XcmI 390 CCAAGATGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1508 CCAACCAGAAGCTGG
XhoII 487 GGATCT [GA]^GATC[CT]
1748 GGATCC
XmaCI 775 CCCGGG C^CCGGG
1496 CCCGGG
XmaI 775 CCCGGG C^CCGGG
1496 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI Ksp632I MaeII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TaqI TfiI TseI TspRI
TthHB8I Van91I