Restriction map for
pFN21AE5733
(Circular) (Six-base) MAPSORT of: pFN21AE5733.seq Check: 2567 from: 1 to: 6631
pFN21AE5733 6631 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3573 3573
Size: 6631
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3384 4441 278
Size: 53 83 186 936 1848 1057 2468
Fragments arranged by size:
2468 1848 1057 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3705 3974 1937
Size: 1768 269 4594
Fragments arranged by size:
4594 1768 269
Acc65I G'GTAC_C
Cuts at: 3952 3952
Size: 6631
AclI AA'CG_TT
Cuts at: 1563 4758 5131 1563
Size: 3195 373 3063
Fragments arranged by size:
3195 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4694 6078 6346 1632
Size: 3062 1384 268 1917
Fragments arranged by size:
3062 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6434
AflIII A'CryG_T
Cuts at: 1203 2226 5530 6507 1203
Size: 1023 3304 977 1327
Fragments arranged by size:
3304 1327 1023 977
AgeI A'CCGG_T
Cuts at: 2148 5462 2148
Size: 3314 3317
AhdI GACnn_n'nnGTC
Cuts at: 5360 5360
Size: 6631
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6631
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6631
AlwNI CAG_nnn'CTG
Cuts at: 2657 2681 2902 3523 4374 5946 2657
Size: 24 221 621 851 1572 3342
Fragments arranged by size:
3342 1572 851 621 221 24
ApaI G_GGCC'C
Cuts at: 2799 2840 2799
Size: 41 6590
ApaLI G'TGCA_C
Cuts at: 4688 5844 4688
Size: 1156 5475
ApoI r'AATT_y
Cuts at: 1493 3940 4166 4318 6427 1493
Size: 2447 226 152 2109 1697
Fragments arranged by size:
2447 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5185 160
Size: 872 4153 1606
Fragments arranged by size:
4153 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
AvaI C'yCGr_G
Cuts at: 1870 1943 2309 2780 3422 3501 3782 3956 1870
Size: 73 366 471 642 79 281 174 4545
Fragments arranged by size:
4545 642 471 366 281 174 79 73
BamHI G'GATC_C
Cuts at: 3753 3961 4328 3753
Size: 208 367 6056
Fragments arranged by size:
6056 367 208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2390 3441 3921 3952
Size: 325 217 627 251 352 1051 480 31
Cuts at: 3952 5407 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1051 627 480 352 325 251
217 31
BanII G_rGCy'C
Cuts at: 1391 2105 2340 2664 2754 2799 2840 3422 3432
Size: 714 235 324 90 45 41 582 10
Cuts at: 3432 3623 3950 1391
Size: 191 327 4072
Fragments arranged by size:
4072 714 582 327 324 235 191 90
45 41 10
BbeI G_GCGC'C
Cuts at: 3925 3925
Size: 6631
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2870 2913 2976 2985 928
Size: 971 971 43 63 9 4574
Fragments arranged by size:
4574 971 971 63 43 9
BbvCI CC'TCA_GC
Cuts at: 2604 3837 2604
Size: 1233 5398
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2576 2610 4822 4856 1903
Size: 34 639 34 2212 34 3678
Fragments arranged by size:
3678 2212 639 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4523 5733 4523
Size: 1210 5421
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6631
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2718 5242 136
Size: 107 122 71 631 605 1046 2524 1525
Fragments arranged by size:
2524 1525 1046 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6626 6626
Size: 6631
BlpI GC'TnA_GC
Cuts at: 3866 4048 3866
Size: 182 6449
Bme1580I G_kGCm'C
Cuts at: 1790 2395 2799 2840 3446 4692 5848 1790
Size: 605 404 41 606 1246 1156 2573
Fragments arranged by size:
2573 1246 1156 606 605 404 41
BmgBI CAC'GTC
Cuts at: 1318 2227 3772 1318
Size: 909 1545 4177
Fragments arranged by size:
4177 1545 909
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4362 5320 442
Size: 416 53 83 399 24 2945 958 1753
Fragments arranged by size:
2945 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2478 1056
Size: 1422 5209
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 6631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2403 2703 2768 2924 2984 3607 3677 3716
Size: 1164 300 65 156 60 623 70 39
Cuts at: 3716 5291 1239
Size: 1575 2579
Fragments arranged by size:
2579 1575 1164 623 300 156 70 65
60 39
Bpu10I CC'TnA_GC
Cuts at: 2339 2604 2658 3617 3837 2339
Size: 265 54 959 220 5133
Fragments arranged by size:
5133 959 265 220 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4756 5621 5919 4756
Size: 865 298 5468
Fragments arranged by size:
5468 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2157 3641 5294 882
Size: 617 658 1484 1653 2219
Fragments arranged by size:
2219 1653 1484 658 617
BsaAI yAC'GTr
Cuts at: 493 1204 3634 4422 493
Size: 711 2430 788 2702
Fragments arranged by size:
2702 2430 788 711
BsaBI GATnn'nnATC
Cuts at: 6625 6625
Size: 6631
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3381 3702 3922 4438
Size: 53 83 186 936 1848 321 220 516
Cuts at: 4438 4820 5455 275
Size: 382 635 1451
Fragments arranged by size:
1848 1451 936 635 516 382 321 220
186 83 53
BsaWI w'CCGG_w
Cuts at: 2148 3291 3750 3756 5064 5462 5736 5883 6294
Size: 1143 459 6 1308 398 274 147 411
Cuts at: 6294 2148
Size: 2485
Fragments arranged by size:
2485 1308 1143 459 411 398 274 147
6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2248 2278 2897 2927 1178
Size: 30 165 30 845 30 619 30 4882
Fragments arranged by size:
4882 845 619 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2173 2194 2269 2999 3440 3854 1186
Size: 987 21 75 730 441 414 3963
Fragments arranged by size:
3963 987 730 441 414 75 21
BseYI C'CCAG_C
Cuts at: 2377 2547 2650 2833 5834 6330 2377
Size: 170 103 183 3001 496 2678
Fragments arranged by size:
3001 2678 496 183 170 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2762 1163
Size: 573 1026 5032
Fragments arranged by size:
5032 1026 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2069 3487 3751 4081 4842 5870
Size: 275 186 68 1418 264 330 761 1028
Cuts at: 5870 1540
Size: 2301
Fragments arranged by size:
2301 1418 1028 761 330 275 264 186
68
BsiHKAI G_wGCw'C
Cuts at: 3623 3732 4692 4777 5848 3623
Size: 109 960 85 1071 4406
Fragments arranged by size:
4406 1071 960 109 85
BsmI GAATG_Cn'
Cuts at: 2693 3042 4142 4235 2693
Size: 349 1100 93 5089
Fragments arranged by size:
5089 1100 349 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2217 2217
Size: 6631
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2340 2395 2664 2754 2799 2840
Size: 399 315 235 55 269 90 45 41
Cuts at: 2840 3422 3432 3446 3623 3732 3950 4692 4777
Size: 582 10 14 177 109 218 742 85
Cuts at: 4777 5848 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 582 399 315 269 235
218 177 109 90 85 55 45 41
14 10
BspEI T'CCGG_A
Cuts at: 6294 6294
Size: 6631
BspHI T'CATG_A
Cuts at: 4520 6250 4520
Size: 1730 4901
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3573 3702 3986 844
Size: 2729 129 284 3489
Fragments arranged by size:
3489 2729 284 129
BsrBI CCG'CTC
Cuts at: 1394 1823 3489 3913 4518 1394
Size: 429 1666 424 605 3507
Fragments arranged by size:
3507 1666 605 429 424
BsrDI GCAATG_nn'
Cuts at: 65 1248 5126 5300 65
Size: 1183 3878 174 1396
Fragments arranged by size:
3878 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2148 3918 5275 5462 1700
Size: 110 338 1770 1357 187 2869
Fragments arranged by size:
2869 1770 1357 338 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6631
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6631
BssSI C'ACGA_G
Cuts at: 4691 5703 4691
Size: 1012 5619
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6631
BstBI TT'CG_AA
Cuts at: 2198 5435 2198
Size: 3237 3394
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6631
BstXI CCAn_nnnn'nTGG
Cuts at: 3600 3600
Size: 6631
BstYI r'GATC_y
Cuts at: 1972 2492 3753 3961 4328 4713 4730 6182 6268
Size: 520 1261 208 367 385 17 1452 86
Cuts at: 6268 6280 6626 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1261 520 385 367 346 208
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2169 2218 2754 2934 3432 513
Size: 552 939 165 49 536 180 498 3712
Fragments arranged by size:
3712 939 552 536 498 180 165 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5892
Fragments arranged by size:
5892 683 56
BtsI GCAGTG_nn'
Cuts at: 2640 3087 4147 4941 4961 2640
Size: 447 1060 794 20 4310
Fragments arranged by size:
4310 1060 794 447 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6631
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2411 2446 529
Size: 35 1847 35 4714
Fragments arranged by size:
4714 1847 35 35
DraI TTT'AAA
Cuts at: 3186 3935 3186
Size: 749 5882
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3287 5638 809
Size: 507 1971 2351 1802
Fragments arranged by size:
2351 1971 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3074 4078 4967
Size: 54 1425 50 275 18 1244 1004 889
Cuts at: 4967 6596 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1244 1004 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4078 1537
Size: 275 2266 4090
Fragments arranged by size:
4090 2266 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2217 2922 3903 4561 1353
Size: 864 705 981 658 3423
Fragments arranged by size:
3423 981 864 705 658
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5218 5592 5738 387
Size: 1064 138 3629 374 146 1280
Fragments arranged by size:
3629 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3621 3621
Size: 6631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2403 2703 2768 2924 2984 3607 3677
Size: 393 771 300 65 156 60 623 70
Cuts at: 3677 3716 4694 5291 6078 6346 1239
Size: 39 978 597 787 268 1524
Fragments arranged by size:
1524 978 787 771 623 597 393 300
268 156 70 65 60 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2504 2504
Size: 6631
EcoO109I rG'GnC_Cy
Cuts at: 2305 2795 3857 2305
Size: 490 1062 5079
Fragments arranged by size:
5079 1062 490
EcoRI G'AATT_C
Cuts at: 3940 4318 3940
Size: 378 6253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 6631
FspI TGC'GCA
Cuts at: 5137 5137
Size: 6631
HaeII r_GCGC'y
Cuts at: 1678 3925 5778 1678
Size: 2247 1853 2531
Fragments arranged by size:
2531 2247 1853
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2098 2130 2186 2218 2393
Size: 32 135 32 526 32 56 32 175
Cuts at: 2393 2425 2903 2935 5272 5304 5346 5378 1373
Size: 32 478 32 2337 32 42 32 2626
Fragments arranged by size:
2626 2337 526 478 175 135 56 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3975 4221 669
Size: 988 281 2037 246 3079
Fragments arranged by size:
3079 2037 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3395 748
Size: 2647 3984
HpaI GTT'AAC
Cuts at: 4221 4221
Size: 6631
KasI G'GCGC_C
Cuts at: 3921 3921
Size: 6631
KpnI G_GTAC'C
Cuts at: 3956 3956
Size: 6631
MfeI C'AATT_G
Cuts at: 4230 4230
Size: 6631
MluI A'CGCG_T
Cuts at: 6507 6507
Size: 6631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5745 5929 1305
Size: 4440 184 2007
Fragments arranged by size:
4440 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3076 10
Size: 54 1425 343 1244 3565
Fragments arranged by size:
3565 1425 1244 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4589 4948 5107 518
Size: 648 3423 359 159 2042
Fragments arranged by size:
3423 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2220 2381 2547 2746 3673 4047 4724 5872
Size: 614 161 166 199 927 374 677 1148
Cuts at: 5872 6117 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 927 677 614 374 245 199
166 161
NarI GG'CG_CC
Cuts at: 3922 3922
Size: 6631
NcoI C'CATG_G
Cuts at: 513 1065 2004 2169 2754 2934 513
Size: 552 939 165 585 180 4210
Fragments arranged by size:
4210 939 585 552 180 165
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6631
NheI G'CTAG_C
Cuts at: 1052 2474 1052
Size: 1422 5209
NotI GC'GGCC_GC
Cuts at: 4078 4078
Size: 6631
NspI r_CATG'y
Cuts at: 1132 1229 2180 3989 5534 1132
Size: 97 951 1809 1545 2229
Fragments arranged by size:
2229 1809 1545 951 97
PasI CC'CwG_GG
Cuts at: 1348 3448 1348
Size: 2100 4531
PciI A'CATG_T
Cuts at: 5530 5530
Size: 6631
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2902 3523 3715 1108
Size: 1794 621 192 4024
Fragments arranged by size:
4024 1794 621 192
PfoI T'CCnGG_A
Cuts at: 2961 3324 2961
Size: 363 6268
PmeI GTTT'AAAC
Cuts at: 3935 3935
Size: 6631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4700 4732 6213 6245 4700
Size: 32 1481 32 5086
Fragments arranged by size:
5086 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 2305
Size: 6631
PshAI GACnn'nnGTC
Cuts at: 1613 2619 1613
Size: 1006 5625
PsiI TTA'TAA
Cuts at: 4201 4201
Size: 6631
PspOMI G'GGCC_C
Cuts at: 2795 2836 2795
Size: 41 6590
PstI C_TGCA'G
Cuts at: 830 2131 3609 3699 3983 830
Size: 1301 1478 90 284 3478
Fragments arranged by size:
3478 1478 1301 284 90
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6631
PvuII CAG'CTG
Cuts at: 2381 2547 2746 3673 2381
Size: 166 199 927 5339
Fragments arranged by size:
5339 927 199 166
SacI G_AGCT'C
Cuts at: 3623 3623
Size: 6631
SacII CC_GC'GG
Cuts at: 2221 2221
Size: 6631
SalI G'TCGA_C
Cuts at: 1936 3973 1936
Size: 2037 4594
SapI GCTCTTCn'nnn_
Cuts at: 3903 3903
Size: 6631
SbfI CC_TGCA'GG
Cuts at: 3699 3983 3699
Size: 284 6347
ScaI AGT'ACT
Cuts at: 4879 6591 4879
Size: 1712 4919
SexAI A'CCwGG_T
Cuts at: 3678 3678
Size: 6631
SfcI C'TryA_G
Cuts at: 826 1045 2059 2127 3283 3605 3695 3979 5114
Size: 219 1014 68 1156 322 90 284 1135
Cuts at: 5114 5795 5986 826
Size: 681 191 1471
Fragments arranged by size:
1471 1156 1135 1014 681 322 284 219
191 90 68
SfoI GGC'GCC
Cuts at: 3923 3923
Size: 6631
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6631
SmaI CCC'GGG
Cuts at: 1872 2782 3503 3958 1872
Size: 910 721 455 4545
Fragments arranged by size:
4545 910 721 455
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4735 5636 5898 820
Size: 197 926 2792 901 262 1553
Fragments arranged by size:
2792 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4422 493
Size: 3929 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6631
SphI G_CATG'C
Cuts at: 1132 3989 1132
Size: 2857 3774
SspI AAT'ATT
Cuts at: 5 52 3332 3888 4555 5
Size: 47 3280 556 667 2081
Fragments arranged by size:
3280 2081 667 556 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6631
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 2754 2934 3546 3723 4070
Size: 552 939 165 585 180 612 177 347
Cuts at: 4070 513
Size: 3074
Fragments arranged by size:
3074 939 612 585 552 347 180 177
165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4658 4675 4828 5013 5458 325
Size: 1255 3078 17 153 185 445 1498
Fragments arranged by size:
3078 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3531 3846 4877
Size: 274 80 33 51 1443 1554 315 1031
Cuts at: 4877 6589 96
Size: 1712 138
Fragments arranged by size:
1712 1554 1443 1031 315 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2248 2280 2297 2329 2897 2929 2982 3014 6591
Size: 32 17 32 568 32 53 32 3577
Cuts at: 6591 6623 2248
Size: 32 2256
Fragments arranged by size:
3577 2256 568 53 32 32 32 32
32 17
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6631
XbaI T'CTAG_A
Cuts at: 3967 3967
Size: 6631
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2402 3520 2402
Size: 1118 5513
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6631
XmaI C'CCGG_G
Cuts at: 1870 2780 3501 3956 1870
Size: 910 721 455 4545
Fragments arranged by size:
4545 910 721 455
XmnI GAAnn'nnTTC
Cuts at: 3161 4760 3161
Size: 1599 5032
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3382 4439 276
Size: 53 83 186 936 1848 1057 2468
Fragments arranged by size:
2468 1848 1057 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SanDI SfiI
SrfI SwaI