(Linear) (Six-base) MAPSORT of: KIEE5733.seq Check: 6291 from: 1 to: 1926 KIEE5733.orf With 182 enzymes: SgfI * December 5, 2014 13:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1568 1926 Size: 1568 358 AatII G_ACGT'C Cuts at: 0 1379 1926 Size: 1379 547 AccI GT'mk_AC Cuts at: 0 1700 1926 Size: 1700 226 AflIII A'CryG_T Cuts at: 0 221 1926 Size: 221 1705 AgeI A'CCGG_T Cuts at: 0 143 1926 Size: 143 1783 AlwNI CAG_nnn'CTG Cuts at: 0 652 676 897 1518 1926 Size: 652 24 221 621 408 Fragments arranged by size: 652 621 408 221 24 ApaI G_GGCC'C Cuts at: 0 794 835 1926 Size: 794 41 1091 Fragments arranged by size: 1091 794 41 AvaI C'yCGr_G Cuts at: 0 304 775 1417 1496 1777 1926 Size: 304 471 642 79 281 149 Fragments arranged by size: 642 471 304 281 149 79 BamHI G'GATC_C Cuts at: 0 1748 1926 Size: 1748 178 BanI G'GyrC_C Cuts at: 0 33 385 1436 1916 1926 Size: 33 352 1051 480 10 Fragments arranged by size: 1051 480 352 33 10 BanII G_rGCy'C Cuts at: 0 100 335 659 749 794 835 1417 1427 Size: 100 235 324 90 45 41 582 10 Cuts at: 1427 1618 1926 Size: 191 308 Fragments arranged by size: 582 324 308 235 191 100 90 45 41 10 BbeI G_GCGC'C Cuts at: 0 1920 1926 Size: 1920 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 865 908 971 980 1926 Size: 865 43 63 9 946 Fragments arranged by size: 946 865 63 43 9 BbvCI CC'TCA_GC Cuts at: 0 599 1832 1926 Size: 599 1233 94 Fragments arranged by size: 1233 599 94 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 571 605 1926 Size: 571 34 1321 Fragments arranged by size: 1321 571 34 BglI GCCn_nnn'nGGC Cuts at: 0 713 1926 Size: 713 1213 BlpI GC'TnA_GC Cuts at: 0 1861 1926 Size: 1861 65 Bme1580I G_kGCm'C Cuts at: 0 390 794 835 1441 1926 Size: 390 404 41 606 485 Fragments arranged by size: 606 485 404 390 41 BmgBI CAC'GTC Cuts at: 0 222 1767 1926 Size: 222 1545 159 Fragments arranged by size: 1545 222 159 BmtI G_CTAG'C Cuts at: 0 473 1926 Size: 473 1453 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 192 1926 Size: 192 1734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 398 698 763 919 979 1602 1672 1711 Size: 398 300 65 156 60 623 70 39 Cuts at: 1711 1926 Size: 215 Fragments arranged by size: 623 398 300 215 156 70 65 60 39 Bpu10I CC'TnA_GC Cuts at: 0 334 599 653 1612 1832 1926 Size: 334 265 54 959 220 94 Fragments arranged by size: 959 334 265 220 94 54 BsaI GGTCTCn'nnnn_ Cuts at: 0 152 1636 1926 Size: 152 1484 290 Fragments arranged by size: 1484 290 152 BsaAI yAC'GTr Cuts at: 0 1629 1926 Size: 1629 297 BsaHI Gr'CG_yC Cuts at: 0 1376 1697 1917 1926 Size: 1376 321 220 9 Fragments arranged by size: 1376 321 220 9 BsaWI w'CCGG_w Cuts at: 0 143 1286 1745 1751 1926 Size: 143 1143 459 6 175 Fragments arranged by size: 1143 459 175 143 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 243 273 892 922 1926 Size: 243 30 619 30 1004 Fragments arranged by size: 1004 619 243 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 168 189 264 994 1435 1849 1926 Size: 168 21 75 730 441 414 77 Fragments arranged by size: 730 441 414 168 77 75 21 BseYI C'CCAG_C Cuts at: 0 372 542 645 828 1926 Size: 372 170 103 183 1098 Fragments arranged by size: 1098 372 183 170 103 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 757 1926 Size: 757 1169 BsiEI CG_ry'CG Cuts at: 0 64 1482 1746 1926 Size: 64 1418 264 180 Fragments arranged by size: 1418 264 180 64 BsiHKAI G_wGCw'C Cuts at: 0 1618 1727 1926 Size: 1618 109 199 Fragments arranged by size: 1618 199 109 BsmI GAATG_Cn' Cuts at: 0 688 1037 1926 Size: 688 349 889 Fragments arranged by size: 889 688 349 BsmBI CGTCTCn'nnnn_ Cuts at: 0 212 1926 Size: 212 1714 Bsp1286I G_dGCh'C Cuts at: 0 100 335 390 659 749 794 835 1417 Size: 100 235 55 269 90 45 41 582 Cuts at: 1417 1427 1441 1618 1727 1926 Size: 10 14 177 109 199 Fragments arranged by size: 582 269 235 199 177 109 100 90 55 45 41 14 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1568 1697 1926 Size: 1568 129 229 Fragments arranged by size: 1568 229 129 BsrBI CCG'CTC Cuts at: 0 1484 1908 1926 Size: 1484 424 18 Fragments arranged by size: 1484 424 18 BsrFI r'CCGG_y Cuts at: 0 143 1913 1926 Size: 143 1770 13 Fragments arranged by size: 1770 143 13 BstBI TT'CG_AA Cuts at: 0 193 1926 Size: 193 1733 BstXI CCAn_nnnn'nTGG Cuts at: 0 1595 1926 Size: 1595 331 BstYI r'GATC_y Cuts at: 0 487 1748 1926 Size: 487 1261 178 Fragments arranged by size: 1261 487 178 BtgI C'CryG_G Cuts at: 0 164 213 749 929 1427 1926 Size: 164 49 536 180 498 499 Fragments arranged by size: 536 499 498 180 164 49 BtsI GCAGTG_nn' Cuts at: 0 635 1082 1926 Size: 635 447 844 Fragments arranged by size: 844 635 447 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 406 441 1926 Size: 406 35 1485 Fragments arranged by size: 1485 406 35 DraI TTT'AAA Cuts at: 0 1181 1926 Size: 1181 745 DrdI GACnn_nn'nnGTC Cuts at: 0 1282 1926 Size: 1282 644 EaeI y'GGCC_r Cuts at: 0 1069 1926 Size: 1069 857 EarI CTCTTCn'nnn_ Cuts at: 0 212 917 1898 1926 Size: 212 705 981 28 Fragments arranged by size: 981 705 212 28 EcoICRI GAG'CTC Cuts at: 0 1616 1926 Size: 1616 310 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 398 698 763 919 979 1602 1672 1711 Size: 398 300 65 156 60 623 70 39 Cuts at: 1711 1926 Size: 215 Fragments arranged by size: 623 398 300 215 156 70 65 60 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 499 1926 Size: 499 1427 EcoO109I rG'GnC_Cy Cuts at: 0 300 790 1852 1926 Size: 300 490 1062 74 Fragments arranged by size: 1062 490 300 74 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 489 1926 Size: 489 1437 HaeII r_GCGC'y Cuts at: 0 1920 1926 Size: 1920 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 93 125 181 213 388 420 898 930 Size: 93 32 56 32 175 32 478 32 Cuts at: 930 1926 Size: 996 Fragments arranged by size: 996 478 175 93 56 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 1390 1926 Size: 1390 536 KasI G'GCGC_C Cuts at: 0 1916 1926 Size: 1916 10 MscI TGG'CCA Cuts at: 0 1071 1926 Size: 1071 855 MspA1I CmG'CkG Cuts at: 0 215 376 542 741 1668 1926 Size: 215 161 166 199 927 258 Fragments arranged by size: 927 258 215 199 166 161 NarI GG'CG_CC Cuts at: 0 1917 1926 Size: 1917 9 NcoI C'CATG_G Cuts at: 0 164 749 929 1926 Size: 164 585 180 997 Fragments arranged by size: 997 585 180 164 NheI G'CTAG_C Cuts at: 0 469 1926 Size: 469 1457 NspI r_CATG'y Cuts at: 0 175 1926 Size: 175 1751 PasI CC'CwG_GG Cuts at: 0 1443 1926 Size: 1443 483 PflMI CCAn_nnn'nTGG Cuts at: 0 897 1518 1710 1926 Size: 897 621 192 216 Fragments arranged by size: 897 621 216 192 PfoI T'CCnGG_A Cuts at: 0 956 1319 1926 Size: 956 363 607 Fragments arranged by size: 956 607 363 PpuMI rG'GwC_Cy Cuts at: 0 300 1926 Size: 300 1626 PshAI GACnn'nnGTC Cuts at: 0 614 1926 Size: 614 1312 PspOMI G'GGCC_C Cuts at: 0 790 831 1926 Size: 790 41 1095 Fragments arranged by size: 1095 790 41 PstI C_TGCA'G Cuts at: 0 126 1604 1694 1926 Size: 126 1478 90 232 Fragments arranged by size: 1478 232 126 90 PvuII CAG'CTG Cuts at: 0 376 542 741 1668 1926 Size: 376 166 199 927 258 Fragments arranged by size: 927 376 258 199 166 SacI G_AGCT'C Cuts at: 0 1618 1926 Size: 1618 308 SacII CC_GC'GG Cuts at: 0 216 1926 Size: 216 1710 SapI GCTCTTCn'nnn_ Cuts at: 0 1898 1926 Size: 1898 28 SbfI CC_TGCA'GG Cuts at: 0 1694 1926 Size: 1694 232 SexAI A'CCwGG_T Cuts at: 0 1673 1926 Size: 1673 253 SfcI C'TryA_G Cuts at: 0 54 122 1278 1600 1690 1926 Size: 54 68 1156 322 90 236 Fragments arranged by size: 1156 322 236 90 68 54 SfoI GGC'GCC Cuts at: 0 1918 1926 Size: 1918 8 SmaI CCC'GGG Cuts at: 0 777 1498 1926 Size: 777 721 428 Fragments arranged by size: 777 721 428 SspI AAT'ATT Cuts at: 0 1327 1883 1926 Size: 1327 556 43 Fragments arranged by size: 1327 556 43 StyI C'CwwG_G Cuts at: 0 164 749 929 1541 1718 1926 Size: 164 585 180 612 177 208 Fragments arranged by size: 612 585 208 180 177 164 TatI w'GTAC_w Cuts at: 0 1526 1841 1926 Size: 1526 315 85 Fragments arranged by size: 1526 315 85 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 243 275 292 324 892 924 977 1009 Size: 243 32 17 32 568 32 53 32 Cuts at: 1009 1926 Size: 917 Fragments arranged by size: 917 568 243 53 32 32 32 32 17 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 397 1515 1926 Size: 397 1118 411 Fragments arranged by size: 1118 411 397 XmaI C'CCGG_G Cuts at: 0 775 1496 1926 Size: 775 721 430 Fragments arranged by size: 775 721 430 XmnI GAAnn'nnTTC Cuts at: 0 1156 1926 Size: 1156 770 ZraI GAC'GTC Cuts at: 0 1377 1926 Size: 1377 549 Enzymes that do cut: AarI AatII AccI AflIII AgeI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BstBI BstXI BstYI BtgI BtsI CspCI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KasI MscI MspA1I NarI NcoI NheI NspI PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SacII SapI SbfI SexAI SfcI SfoI SmaI SspI StyI TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AcuI AfeI AflII AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmrI BpuEI BsaBI BsiWI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI DraIII EagI EciI EcoRI EcoRV FseI FspI FspAI HincII HpaI KpnI MfeI MluI MmeI MslI NaeI NdeI NgoMIV NotI NruI NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SalI SanDI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII Tth111I XbaI XhoI