################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KspI MamI MfeI MluNI MscI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 799 GGTACC G^GTACC AccII 30 CGCG CG^CG AccIII 110 TCCGGA T^CCGGA AclI 467 AACGTT AA^CGTT AcsI 523 AAATTC [GA]^AATT[CT] AflIII 29 ACGCGT A^C[GA][CT]GT Alw44I 21 GTGCAC G^TGCAC AlwNI 2039 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 21 GTGCAC G^TGCAC ApoI 523 AAATTC [GA]^AATT[CT] Asp718I 799 GGTACC G^GTACC AvaI 1463 CTCGAG C^[CT]CG[GA]G BbsI 1989 GAAGAC GAAGAC(2/6) BciVI 1429 GGATAC GTATCC(6/5) BglII 2020 AGATCT A^GATCT BpuAI 1989 GAAGAC GAAGAC(2/6) BsaI 1677 GAGACC GGTCTC(1/5) BsaWI 110 TCCGGA [AT]^CCGG[AT] BseAI 110 TCCGGA T^CCGGA BsoBI 1463 CTCGAG C^[CT]CG[GA]G BspEI 110 TCCGGA T^CCGGA BspMI 1283 ACCTGC ACCTGC(4/8) Bst1107I 1233 GTATAC GTA^TAC BstUI 30 CGCG CG^CG BstZ17I 1233 GTATAC GTA^TAC DsaI 1138 CCATGG C^C[GA][CT]GG EcoNI 784 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 172 GATATC GAT^ATC EcoT14I 1138 CCATGG C^C[AT][AT]GG HgaI 28 GACGC GACGC(5/10) HincII 550 GTCAAC GT[CT]^[GA]AC HindII 550 GTCAAC GT[CT]^[GA]AC KpnI 799 GGTACC GGTAC^C MluI 29 ACGCGT A^CGCGT MroI 110 TCCGGA T^CCGGA MvnI 30 CGCG CG^CG NcoI 1138 CCATGG C^CATGG PaeR7I 1463 CTCGAG C^TCGAG PflMI 1150 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 467 AACGTT AA^CGTT SacI 955 GAGCTC GAGCT^C StyI 1138 CCATGG C^C[AT][AT]GG Van91I 1150 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1463 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 1178 GTCTAC GT^[AC][GT]AC 1233 GTATAC AocI 396 CCTAAGG CC^T[ACGT]AGG 1938 CCTGAGG BcnI 102 CCGGG CC^[GC]GG 168 CCCGG BsgI 808 CTGCAC GTGCAG(16/14) 1050 GTGCAG BsmI 840 GAATGC GAATGC(1/-1) 106 GCATTC Bsu36I 396 CCTAAGG CC^T[ACGT]AGG 1938 CCTGAGG DraII 1721 AGGACCC [GA]G^G[ACGT]CC[CT] 1875 GGGTCCT EaeI 287 CGGCCA [CT]^GGCC[GA] 725 CGGCCA EarI 533 CTCTTC CTCTTC(1/4) 1000 GAAGAG Eco81I 396 CCTAAGG CC^T[ACGT]AGG 1938 CCTGAGG EcoO109I 1721 AGGACCC [GA]G^G[ACGT]CC[CT] 1875 GGGTCCT FauI 595 CCCGC CCCGC(4/6) 1681 CCCGC Ksp632I 533 CTCTTC CTCTTC(1/4) 1000 GAAGAG NciI 102 CCGGG CC^[GC]GG 168 CCCGG PpuMI 1721 AGGACCC [GA]G^G[AT]CC[CT] 1875 GGGTCCT PstI 990 CTGCAG CTGCA^G 1751 CTGCAG Tsp509I 438 AATT ^AATT 524 AATT XcmI 225 CCAGAGGAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1998 CCAGCTAGAGAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BamHI BanI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I XhoII