(Circular) (Six-base) MAPSORT of: pFN21AE2670.seq Check: 4659 from: 1 to: 6838 pFN21AE2670 6838 bp With 182 enzymes: SgfI * February 2, 2017 15:09 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4648 278 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3184 3239 4181 1937 Size: 1247 55 942 4594 Fragments arranged by size: 4594 1247 942 55 Acc65I G'GTAC_C Cuts at: 2804 4159 2804 Size: 1355 5483 AclI AA'CG_TT Cuts at: 1563 2473 4965 5338 1563 Size: 910 2492 373 3063 Fragments arranged by size: 3063 2492 910 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2625 4052 4901 6285 6553 1632 Size: 993 1427 849 1384 268 1917 Fragments arranged by size: 1917 1427 1384 993 849 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6641 AflIII A'CryG_T Cuts at: 1203 2034 5737 6714 1203 Size: 831 3703 977 1327 Fragments arranged by size: 3703 1327 977 831 AgeI A'CCGG_T Cuts at: 5669 5669 Size: 6838 AhdI GACnn_n'nnGTC Cuts at: 5567 5567 Size: 6838 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6838 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6838 AlwNI CAG_nnn'CTG Cuts at: 4049 4581 6153 4049 Size: 532 1572 4734 Fragments arranged by size: 4734 1572 532 ApaLI G'TGCA_C Cuts at: 2026 4895 6051 2026 Size: 2869 1156 2813 Fragments arranged by size: 2869 2813 1156 ApoI r'AATT_y Cuts at: 1493 2528 4147 4373 4525 6634 1493 Size: 1035 1619 226 152 2109 1697 Fragments arranged by size: 2109 1697 1619 1035 226 152 AseI AT'TA_AT Cuts at: 160 1032 5392 160 Size: 872 4360 1606 Fragments arranged by size: 4360 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 AvaI C'yCGr_G Cuts at: 1870 1943 3468 4163 1870 Size: 73 1525 695 4545 Fragments arranged by size: 4545 1525 695 73 BamHI G'GATC_C Cuts at: 3061 3890 3961 4168 4535 3061 Size: 829 71 207 367 5364 Fragments arranged by size: 5364 829 367 207 71 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2386 2596 2804 4159 5614 Size: 325 217 627 599 210 208 1355 1455 Cuts at: 5614 618 Size: 1842 Fragments arranged by size: 1842 1455 1355 627 599 325 217 210 208 BanII G_rGCy'C Cuts at: 1391 2964 3031 3073 3453 3753 4020 4157 1391 Size: 1573 67 42 380 300 267 137 4072 Fragments arranged by size: 4072 1573 380 300 267 137 67 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4001 928 Size: 971 2102 3765 Fragments arranged by size: 3765 2102 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5029 5063 1903 Size: 34 3092 34 3678 Fragments arranged by size: 3678 3092 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3428 4730 5940 3428 Size: 1302 1210 4326 Fragments arranged by size: 4326 1302 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6838 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5449 136 Size: 107 122 71 631 605 3777 1525 Fragments arranged by size: 3777 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4025 6833 4025 Size: 2808 4030 BlpI GC'TnA_GC Cuts at: 4255 4255 Size: 6838 Bme1580I G_kGCm'C Cuts at: 1790 2030 2273 2601 4899 6055 1790 Size: 240 243 328 2298 1156 2573 Fragments arranged by size: 2573 2298 1156 328 243 240 BmgBI CAC'GTC Cuts at: 1318 3520 1318 Size: 2202 4636 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3447 3738 4569 Size: 416 53 83 399 24 2030 291 831 Cuts at: 4569 5527 442 Size: 958 1753 Fragments arranged by size: 2030 1753 958 831 416 399 291 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2035 2402 2683 2888 3895 3993 4006 5498 Size: 796 367 281 205 1007 98 13 1492 Cuts at: 5498 1239 Size: 2579 Fragments arranged by size: 2579 1492 1007 796 367 281 205 98 13 Bpu10I CC'TnA_GC Cuts at: 2958 3966 2958 Size: 1008 5830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3105 3497 3668 4963 5828 6126 3105 Size: 392 171 1295 865 298 3817 Fragments arranged by size: 3817 1295 865 392 298 171 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3676 5501 882 Size: 617 2177 1825 2219 Fragments arranged by size: 2219 2177 1825 617 BsaAI yAC'GTr Cuts at: 493 1204 4629 493 Size: 711 3425 2702 Fragments arranged by size: 3425 2702 711 BsaBI GATnn'nnATC Cuts at: 6832 6832 Size: 6838 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4645 5027 5662 275 Size: 53 83 186 936 3112 382 635 1451 Fragments arranged by size: 3112 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2115 5271 5669 5943 6090 6501 2115 Size: 3156 398 274 147 411 2452 Fragments arranged by size: 3156 2452 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2956 2986 3060 3090 1178 Size: 30 165 30 1553 30 74 30 4926 Fragments arranged by size: 4926 1553 165 74 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2038 2389 2526 1186 Size: 852 351 137 5498 Fragments arranged by size: 5498 852 351 137 BseYI C'CCAG_C Cuts at: 2286 2713 3101 3135 3359 6041 6537 2286 Size: 427 388 34 224 2682 496 2587 Fragments arranged by size: 2682 2587 496 427 388 224 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2798 3076 1163 Size: 573 1062 278 4925 Fragments arranged by size: 4925 1062 573 278 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4288 5049 6077 1540 Size: 275 186 2287 761 1028 2301 Fragments arranged by size: 2301 2287 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2030 2964 3542 4899 4984 6055 2030 Size: 934 578 1357 85 1071 2813 Fragments arranged by size: 2813 1357 1071 934 578 85 BsmI GAATG_Cn' Cuts at: 2111 2851 4349 4442 2111 Size: 740 1498 93 4507 Fragments arranged by size: 4507 1498 740 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2273 2601 2964 3031 3073 3453 Size: 399 240 243 328 363 67 42 380 Cuts at: 3453 3542 3753 4020 4157 4899 4984 6055 1391 Size: 89 211 267 137 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 380 363 328 267 243 240 211 137 89 85 67 42 BspEI T'CCGG_A Cuts at: 2115 6501 2115 Size: 4386 2452 BspHI T'CATG_A Cuts at: 4727 6457 4727 Size: 1730 5108 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3297 4126 4193 844 Size: 2453 829 67 3489 Fragments arranged by size: 3489 2453 829 67 BsrBI CCG'CTC Cuts at: 1394 1823 4725 1394 Size: 429 2902 3507 Fragments arranged by size: 3507 2902 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5333 5507 65 Size: 1183 4085 174 1396 Fragments arranged by size: 4085 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5482 5669 1700 Size: 110 3672 187 2869 Fragments arranged by size: 3672 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6838 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6838 BssSI C'ACGA_G Cuts at: 4898 5910 4898 Size: 1012 5826 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6838 BstBI TT'CG_AA Cuts at: 5642 5642 Size: 6838 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6838 BstYI r'GATC_y Cuts at: 1972 3061 3890 3961 4025 4115 4168 4535 4920 Size: 1089 829 71 64 90 53 367 385 Cuts at: 4920 4937 6389 6475 6487 6833 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1089 829 385 367 346 90 86 71 64 53 17 12 BstZ17I GTA'TAC Cuts at: 3240 3240 Size: 6838 Bsu36I CC'TnA_GG Cuts at: 2402 3944 2402 Size: 1542 5296 BtgI C'CryG_G Cuts at: 513 1065 3143 513 Size: 552 2078 4208 Fragments arranged by size: 4208 2078 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6099 Fragments arranged by size: 6099 683 56 BtsI GCAGTG_nn' Cuts at: 2490 3699 4354 5148 5168 2490 Size: 1209 655 794 20 4160 Fragments arranged by size: 4160 1209 794 655 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6838 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6803 DraI TTT'AAA Cuts at: 4142 4142 Size: 6838 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5845 809 Size: 507 4529 1802 Fragments arranged by size: 4529 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2292 2730 4285 Size: 54 1425 50 275 18 462 438 1555 Cuts at: 4285 5174 6803 8 Size: 889 1629 43 Fragments arranged by size: 1629 1555 1425 889 462 438 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4285 1537 Size: 275 2473 4090 Fragments arranged by size: 4090 2473 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2544 3000 4768 1353 Size: 1191 456 1768 3423 Fragments arranged by size: 3423 1768 1191 456 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5425 5799 5945 387 Size: 1064 138 3836 374 146 1280 Fragments arranged by size: 3836 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2962 2962 Size: 6838 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2035 2402 2625 2683 2888 3895 3993 Size: 393 403 367 223 58 205 1007 98 Cuts at: 3993 4006 4052 4901 5498 6285 6553 1239 Size: 13 46 849 597 787 268 1524 Fragments arranged by size: 1524 1007 849 787 597 403 393 367 268 223 205 98 58 46 13 EcoNI CCTnn'n_nnAGG Cuts at: 2793 2793 Size: 6838 EcoO109I rG'GnC_Cy Cuts at: 3727 3881 3727 Size: 154 6684 EcoRI G'AATT_C Cuts at: 4147 4525 4147 Size: 378 6460 EcoRV GAT'ATC Cuts at: 2179 2179 Size: 6838 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4082 4082 Size: 6838 FspI TGC'GCA Cuts at: 5344 5344 Size: 6838 HaeII r_GCGC'y Cuts at: 1678 5985 1678 Size: 4307 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2126 2158 5479 5511 5553 Size: 32 135 32 554 32 3321 32 42 Cuts at: 5553 5585 1373 Size: 32 2626 Fragments arranged by size: 3321 2626 554 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2557 4182 4428 669 Size: 988 281 619 1625 246 3079 Fragments arranged by size: 3079 1625 988 619 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6838 HpaI GTT'AAC Cuts at: 4428 4428 Size: 6838 KpnI G_GTAC'C Cuts at: 2808 4163 2808 Size: 1355 5483 MfeI C'AATT_G Cuts at: 4437 4437 Size: 6838 MluI A'CGCG_T Cuts at: 2034 6714 2034 Size: 4680 2158 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2049 2326 2637 3812 3852 5952 6136 1305 Size: 744 277 311 1175 40 2100 184 2007 Fragments arranged by size: 2100 2007 1175 744 311 277 184 40 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5016 Fragments arranged by size: 5016 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4796 5155 5314 518 Size: 648 3630 359 159 2042 Fragments arranged by size: 3630 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2603 2885 2994 3689 3737 3897 4254 4931 Size: 997 282 109 695 48 160 357 677 Cuts at: 4931 6079 6324 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 997 695 677 357 282 245 160 109 48 NcoI C'CATG_G Cuts at: 513 1065 3143 513 Size: 552 2078 4208 Fragments arranged by size: 4208 2078 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6838 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6838 NotI GC'GGCC_GC Cuts at: 4285 4285 Size: 6838 NspI r_CATG'y Cuts at: 1132 1229 4196 5741 1132 Size: 97 2967 1545 2229 Fragments arranged by size: 2967 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3048 1348 Size: 1700 5138 PciI A'CATG_T Cuts at: 5737 5737 Size: 6838 PflMI CCAn_nnn'nTGG Cuts at: 1108 3161 1108 Size: 2053 4785 PfoI T'CCnGG_A Cuts at: 3064 3064 Size: 6838 PmeI GTTT'AAAC Cuts at: 4142 4142 Size: 6838 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2735 2767 4907 4939 6420 6452 2735 Size: 32 2140 32 1481 32 3121 Fragments arranged by size: 3121 2140 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3727 3881 3727 Size: 154 6684 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6838 PsiI TTA'TAA Cuts at: 4408 4408 Size: 6838 PstI C_TGCA'G Cuts at: 830 2999 3760 4190 830 Size: 2169 761 430 3478 Fragments arranged by size: 3478 2169 761 430 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6838 PvuII CAG'CTG Cuts at: 2885 2994 3737 3897 2885 Size: 109 743 160 5826 Fragments arranged by size: 5826 743 160 109 SacI G_AGCT'C Cuts at: 2964 2964 Size: 6838 SalI G'TCGA_C Cuts at: 1936 4180 1936 Size: 2244 4594 SbfI CC_TGCA'GG Cuts at: 4190 4190 Size: 6838 ScaI AGT'ACT Cuts at: 5086 6798 5086 Size: 1712 5126 SfcI C'TryA_G Cuts at: 826 1045 2995 3732 3756 3981 4186 5321 6002 Size: 219 1950 737 24 225 205 1135 681 Cuts at: 6002 6193 826 Size: 191 1471 Fragments arranged by size: 1950 1471 1135 737 681 225 219 205 191 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6838 SmaI CCC'GGG Cuts at: 1872 4165 1872 Size: 2293 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3120 3468 3512 3647 4942 5843 Size: 197 926 1177 348 44 135 1295 901 Cuts at: 5843 6105 820 Size: 262 1553 Fragments arranged by size: 1553 1295 1177 926 901 348 262 197 135 44 SnaBI TAC'GTA Cuts at: 493 4629 493 Size: 4136 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6838 SphI G_CATG'C Cuts at: 1132 4196 1132 Size: 3064 3774 SspI AAT'ATT Cuts at: 5 52 4762 5 Size: 47 4710 2081 Fragments arranged by size: 4710 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6838 StyI C'CwwG_G Cuts at: 513 1065 3143 4277 513 Size: 552 2078 1134 3074 Fragments arranged by size: 3074 2078 1134 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2243 2477 2633 4865 4882 5035 5220 Size: 1255 663 234 156 2232 17 153 185 Cuts at: 5220 5665 325 Size: 445 1498 Fragments arranged by size: 2232 1498 1255 663 445 234 185 156 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5084 6796 96 Size: 274 80 33 51 1443 3107 1712 138 Fragments arranged by size: 3107 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2956 2988 6798 6830 2956 Size: 32 3810 32 2964 Fragments arranged by size: 3810 2964 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6838 XbaI T'CTAG_A Cuts at: 4174 4174 Size: 6838 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2237 4010 2237 Size: 1773 5065 XhoI C'TCGA_G Cuts at: 1943 3468 1943 Size: 1525 5313 XmaI C'CCGG_G Cuts at: 1870 4163 1870 Size: 2293 4545 XmnI GAAnn'nnTTC Cuts at: 4967 4967 Size: 6838 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4646 276 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI