(Linear) (Six-base) MAPSORT of: KIEE2670.seq Check: 6702 from: 1 to: 2133 KIEE2670.orf With 182 enzymes: SgfI * February 2, 2017 14:35 .. AccI GT'mk_AC Cuts at: 0 1179 1234 2133 Size: 1179 55 899 Fragments arranged by size: 1179 899 55 Acc65I G'GTAC_C Cuts at: 0 799 2133 Size: 799 1334 AclI AA'CG_TT Cuts at: 0 468 2133 Size: 468 1665 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 620 2047 2133 Size: 620 1427 86 Fragments arranged by size: 1427 620 86 AflIII A'CryG_T Cuts at: 0 29 2133 Size: 29 2104 AlwNI CAG_nnn'CTG Cuts at: 0 2044 2133 Size: 2044 89 ApaLI G'TGCA_C Cuts at: 0 21 2133 Size: 21 2112 ApoI r'AATT_y Cuts at: 0 523 2133 Size: 523 1610 AvaI C'yCGr_G Cuts at: 0 1463 2133 Size: 1463 670 BamHI G'GATC_C Cuts at: 0 1056 1885 1956 2133 Size: 1056 829 71 177 Fragments arranged by size: 1056 829 177 71 BanI G'GyrC_C Cuts at: 0 381 591 799 2133 Size: 381 210 208 1334 Fragments arranged by size: 1334 381 210 208 BanII G_rGCy'C Cuts at: 0 959 1026 1068 1448 1748 2015 2133 Size: 959 67 42 380 300 267 118 Fragments arranged by size: 959 380 300 267 118 67 42 BbsI GAAGACnn'nnnn_ Cuts at: 0 1996 2133 Size: 1996 137 BciVI GTATCCnnnnn_n' Cuts at: 0 1423 2133 Size: 1423 710 BglII A'GATC_T Cuts at: 0 2020 2133 Size: 2020 113 Bme1580I G_kGCm'C Cuts at: 0 25 268 596 2133 Size: 25 243 328 1537 Fragments arranged by size: 1537 328 243 25 BmgBI CAC'GTC Cuts at: 0 1515 2133 Size: 1515 618 BmrI ACTGGGnnnn_n' Cuts at: 0 1442 1733 2133 Size: 1442 291 400 Fragments arranged by size: 1442 400 291 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 30 397 678 883 1890 1988 2001 2133 Size: 30 367 281 205 1007 98 13 132 Fragments arranged by size: 1007 367 281 205 132 98 30 13 Bpu10I CC'TnA_GC Cuts at: 0 953 1961 2133 Size: 953 1008 172 Fragments arranged by size: 1008 953 172 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1100 1492 1663 2133 Size: 1100 392 171 470 Fragments arranged by size: 1100 470 392 171 BsaI GGTCTCn'nnnn_ Cuts at: 0 1671 2133 Size: 1671 462 BsaWI w'CCGG_w Cuts at: 0 110 2133 Size: 110 2023 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 951 981 1055 1085 2133 Size: 951 30 74 30 1048 Fragments arranged by size: 1048 951 74 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 33 384 521 2133 Size: 33 351 137 1612 Fragments arranged by size: 1612 351 137 33 BseYI C'CCAG_C Cuts at: 0 281 708 1096 1130 1354 2133 Size: 281 427 388 34 224 779 Fragments arranged by size: 779 427 388 281 224 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 793 1071 2133 Size: 793 278 1062 Fragments arranged by size: 1062 793 278 BsiHKAI G_wGCw'C Cuts at: 0 25 959 1537 2133 Size: 25 934 578 596 Fragments arranged by size: 934 596 578 25 BsmI GAATG_Cn' Cuts at: 0 106 846 2133 Size: 106 740 1287 Fragments arranged by size: 1287 740 106 Bsp1286I G_dGCh'C Cuts at: 0 25 268 596 959 1026 1068 1448 1537 Size: 25 243 328 363 67 42 380 89 Cuts at: 1537 1748 2015 2133 Size: 211 267 118 Fragments arranged by size: 380 363 328 267 243 211 118 89 67 42 25 BspEI T'CCGG_A Cuts at: 0 110 2133 Size: 110 2023 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1292 2133 Size: 1292 841 BstYI r'GATC_y Cuts at: 0 1056 1885 1956 2020 2110 2133 Size: 1056 829 71 64 90 23 Fragments arranged by size: 1056 829 90 71 64 23 BstZ17I GTA'TAC Cuts at: 0 1235 2133 Size: 1235 898 Bsu36I CC'TnA_GG Cuts at: 0 397 1939 2133 Size: 397 1542 194 Fragments arranged by size: 1542 397 194 BtgI C'CryG_G Cuts at: 0 1138 2133 Size: 1138 995 BtsI GCAGTG_nn' Cuts at: 0 485 1694 2133 Size: 485 1209 439 Fragments arranged by size: 1209 485 439 EaeI y'GGCC_r Cuts at: 0 287 725 2133 Size: 287 438 1408 Fragments arranged by size: 1408 438 287 EarI CTCTTCn'nnn_ Cuts at: 0 539 995 2133 Size: 539 456 1138 Fragments arranged by size: 1138 539 456 EcoICRI GAG'CTC Cuts at: 0 957 2133 Size: 957 1176 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 30 397 620 678 883 1890 1988 2001 Size: 30 367 223 58 205 1007 98 13 Cuts at: 2001 2047 2133 Size: 46 86 Fragments arranged by size: 1007 367 223 205 98 86 58 46 30 13 EcoNI CCTnn'n_nnAGG Cuts at: 0 788 2133 Size: 788 1345 EcoO109I rG'GnC_Cy Cuts at: 0 1722 1876 2133 Size: 1722 154 257 Fragments arranged by size: 1722 257 154 EcoRV GAT'ATC Cuts at: 0 174 2133 Size: 174 1959 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2077 2133 Size: 2077 56 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 121 153 2133 Size: 121 32 1980 Fragments arranged by size: 1980 121 32 HincII GTy'rAC Cuts at: 0 552 2133 Size: 552 1581 KpnI G_GTAC'C Cuts at: 0 803 2133 Size: 803 1330 MluI A'CGCG_T Cuts at: 0 29 2133 Size: 29 2104 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 44 321 632 1807 1847 2133 Size: 44 277 311 1175 40 286 Fragments arranged by size: 1175 311 286 277 44 40 MspA1I CmG'CkG Cuts at: 0 598 880 989 1684 1732 1892 2133 Size: 598 282 109 695 48 160 241 Fragments arranged by size: 695 598 282 241 160 109 48 NcoI C'CATG_G Cuts at: 0 1138 2133 Size: 1138 995 PasI CC'CwG_GG Cuts at: 0 1043 2133 Size: 1043 1090 PflMI CCAn_nnn'nTGG Cuts at: 0 1156 2133 Size: 1156 977 PfoI T'CCnGG_A Cuts at: 0 1059 2133 Size: 1059 1074 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 730 762 2133 Size: 730 32 1371 Fragments arranged by size: 1371 730 32 PpuMI rG'GwC_Cy Cuts at: 0 1722 1876 2133 Size: 1722 154 257 Fragments arranged by size: 1722 257 154 PstI C_TGCA'G Cuts at: 0 994 1755 2133 Size: 994 761 378 Fragments arranged by size: 994 761 378 PvuII CAG'CTG Cuts at: 0 880 989 1732 1892 2133 Size: 880 109 743 160 241 Fragments arranged by size: 880 743 241 160 109 SacI G_AGCT'C Cuts at: 0 959 2133 Size: 959 1174 SfcI C'TryA_G Cuts at: 0 990 1727 1751 1976 2133 Size: 990 737 24 225 157 Fragments arranged by size: 990 737 225 157 24 SmlI C'TyrA_G Cuts at: 0 1115 1463 1507 1642 2133 Size: 1115 348 44 135 491 Fragments arranged by size: 1115 491 348 135 44 StyI C'CwwG_G Cuts at: 0 1138 2133 Size: 1138 995 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 238 472 628 2133 Size: 238 234 156 1505 Fragments arranged by size: 1505 238 234 156 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 951 983 2133 Size: 951 32 1150 Fragments arranged by size: 1150 951 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 232 2005 2133 Size: 232 1773 128 Fragments arranged by size: 1773 232 128 XhoI C'TCGA_G Cuts at: 0 1463 2133 Size: 1463 670 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI BciVI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BstYI BstZ17I Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII KpnI MluI MmeI MspA1I NcoI PasI PflMI PfoI PpiI PpuMI PstI PvuII SacI SfcI SmlI StyI TaqII TstI XcmI XhoI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AleI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BglI BlpI BmtI BplI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MscI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XmaI XmnI ZraI