################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AfaI AflII AgeI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MamI MfeI MflI MluNI MroI MscI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacI SalI SapI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI SwaI TaqI Tsp509I TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 679 GACGTC GACGT^C AflIII 385 ACGCGT A^C[GA][CT]GT AhdI 632 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 547 AGCT AG^CT Alw44I 83 GTGCAC G^TGCAC AlwI 743 GGATC GGATC(4/5) ApaLI 83 GTGCAC G^TGCAC AspEI 632 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 261 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 230 TGCGCA TGC^GCA BanI 557 GGCGCC G^G[CT][GA]CC BanII 465 GAGCCC G[GA]GC[CT]^C BbeI 557 GGCGCC GGCGC^C BfaI 334 CTAG C^TAG BseRI 10 CTCCTC GAGGAG(10/8) BsgI 581 GTGCAG GTGCAG(16/14) BsmBI 266 CGTCTC CGTCTC(1/5) BspHI 200 TCATGA T^CATGA BspMI 227 ACCTGC ACCTGC(4/8) BsrI 164 ACTGG ACTGG(1/-1) BssHII 298 GCGCGC G^CGCGC BstAPI 222 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DpnII 744 GATC ^GATC DraIII 163 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 632 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 759 GAAGAG CTCTTC(1/4) FspI 230 TGCGCA TGC^GCA HgaI 128 GACGC GACGC(5/10) KasI 557 GGCGCC G^GCGCC Ksp632I 759 GAAGAG CTCTTC(1/4) KspI 371 CCGCGG CCGC^GG MaeI 334 CTAG C^TAG MboI 744 GATC ^GATC MboII 759 GAAGA GAAGA(8/7) MluI 385 ACGCGT A^CGCGT MseI 134 TTAA T^TAA NarI 557 GGCGCC GG^CGCC NdeII 744 GATC ^GATC PflFI 261 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 729 CCACTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 630 GGGACCC [GA]G^G[AT]CC[CT] PstI 655 CTGCAG CTGCA^G RcaI 200 TCATGA T^CATGA SacII 371 CCGCGG CCGC^GG Sau3AI 744 GATC ^GATC SbfI 654 CCTGCAGG CCTGCA^GG SfcI 655 CTGCAG C^T[GA][CT]AG SfoI 557 GGCGCC GGC^GCC SmaI 399 CCCGGG CCC^GGG Sse8387I 654 CCTGCAGG CCTGCA^GG StuI 407 AGGCCT AGG^CCT TfiI 607 GAATC G^A[AT]TC Tru9I 134 TTAA T^TAA Tth111I 261 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 729 CCACTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 441 CCACAACGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 399 CCCGGG C^CCGGG XmaI 399 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 557 GGCGCC G[GA]^CG[CT]C 679 GACGTC AspHI 83 GTGCAC G[AT]GC[AT]^C 220 GAGCAC AvaI 269 CTCGGG C^[CT]CG[GA]G 399 CCCGGG BsaHI 557 GGCGCC G[GA]^CG[CT]C 679 GACGTC BsiHKAI 83 GTGCAC G[AT]GC[AT]^C 220 GAGCAC BsmAI 267 GTCTC GTCTC(1/5) 485 GTCTC BsoBI 269 CTCGGG C^[CT]CG[GA]G 399 CCCGGG DraII 428 GGGGCCT [GA]G^G[ACGT]CC[CT] 630 GGGACCC EcoO109I 428 GGGGCCT [GA]G^G[ACGT]CC[CT] 630 GGGACCC EcoT14I 100 CCATGG C^C[AT][AT]GG 572 CCATGG HaeII 142 GGCGCT [GA]GCGC^[CT] 557 GGCGCC HapII 400 CCGG C^CGG 471 CCGG Hin1I 557 GGCGCC G[GA]^CG[CT]C 679 GACGTC HpaII 400 CCGG C^CGG 471 CCGG MslI 201 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 570 CACCATGGTG MspA1I 371 CCGCGG C[AC]G^C[GT]G 458 CCGCTG MspI 400 CCGG C^CGG 471 CCGG NcoI 100 CCATGG C^CATGG 572 CCATGG PleI 622 GACTC GAGTC(4/5) 483 GAGTC SfaNI 34 GCATC GCATC(5/9) 650 GCATC StyI 100 CCATGG C^C[AT][AT]GG 572 CCATGG TspRI 163 CACTG CA[GC]TG[ACGT][ACGT]^ 184 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AccII AciI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DsaI EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeII MaeIII MnlI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI Tsp45I