(Circular) (Six-base) MAPSORT of: pFN21AE5258.seq Check: 7351 from: 1 to: 5473 pFN21AE5258 5473 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2688 3283 278 Size: 53 83 186 936 1152 595 2468 Fragments arranged by size: 2468 1152 936 595 186 83 53 AccI GT'mk_AC Cuts at: 1937 2816 1937 Size: 879 4594 Acc65I G'GTAC_C Cuts at: 2794 2794 Size: 5473 AclI AA'CG_TT Cuts at: 1563 2772 3600 3973 1563 Size: 1209 828 373 3063 Fragments arranged by size: 3063 1209 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3536 4920 5188 1632 Size: 1904 1384 268 1917 Fragments arranged by size: 1917 1904 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5276 AflIII A'CryG_T Cuts at: 1203 2390 4372 5349 1203 Size: 1187 1982 977 1327 Fragments arranged by size: 1982 1327 1187 977 AgeI A'CCGG_T Cuts at: 4304 4304 Size: 5473 AhdI GACnn_n'nnGTC Cuts at: 2642 4202 2642 Size: 1560 3913 AleI CACnn'nnGTG Cuts at: 1166 2579 1166 Size: 1413 4060 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5473 AlwNI CAG_nnn'CTG Cuts at: 3216 4788 3216 Size: 1572 3901 ApaLI G'TGCA_C Cuts at: 2088 3530 4686 2088 Size: 1442 1156 2875 Fragments arranged by size: 2875 1442 1156 ApoI r'AATT_y Cuts at: 1493 2782 3008 3160 5269 1493 Size: 1289 226 152 2109 1697 Fragments arranged by size: 2109 1697 1289 226 152 AseI AT'TA_AT Cuts at: 160 1032 4027 160 Size: 872 2995 1606 Fragments arranged by size: 2995 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 AvaI C'yCGr_G Cuts at: 1870 1943 2274 2404 2798 1870 Size: 73 331 130 394 4545 Fragments arranged by size: 4545 394 331 130 73 BamHI G'GATC_C Cuts at: 2803 3170 2803 Size: 367 5106 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2562 2794 4249 618 Size: 325 217 627 775 232 1455 1842 Fragments arranged by size: 1842 1455 775 627 325 232 217 BanII G_rGCy'C Cuts at: 1391 2474 2792 1391 Size: 1083 318 4072 Fragments arranged by size: 4072 1083 318 BbeI G_GCGC'C Cuts at: 2566 2566 Size: 5473 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4502 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3664 3698 1903 Size: 34 1727 34 3678 Fragments arranged by size: 3678 1727 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3365 4575 3365 Size: 1210 4263 BclI T'GATC_A Cuts at: 1548 1548 Size: 5473 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4084 136 Size: 107 122 71 631 605 2412 1525 Fragments arranged by size: 2412 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5468 5468 Size: 5473 BlpI GC'TnA_GC Cuts at: 2890 2890 Size: 5473 Bme1580I G_kGCm'C Cuts at: 1790 2092 3534 4690 1790 Size: 302 1442 1156 2573 Fragments arranged by size: 2573 1442 1156 302 BmgBI CAC'GTC Cuts at: 1318 2271 1318 Size: 953 4520 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2179 3204 4162 Size: 416 53 83 399 24 762 1025 958 Cuts at: 4162 442 Size: 1753 Fragments arranged by size: 1753 1025 958 762 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5473 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2644 2644 Size: 5473 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4133 1239 Size: 2894 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3598 4463 4761 3598 Size: 865 298 4310 Fragments arranged by size: 4310 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4136 882 Size: 617 2637 2219 Fragments arranged by size: 2637 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3264 493 Size: 711 2060 2702 Fragments arranged by size: 2702 2060 711 BsaBI GATnn'nnATC Cuts at: 5467 5467 Size: 5473 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2563 2685 3280 3662 Size: 53 83 186 936 1030 122 595 382 Cuts at: 3662 4297 275 Size: 635 1451 Fragments arranged by size: 1451 1030 936 635 595 382 186 122 83 53 BsaWI w'CCGG_w Cuts at: 3906 4304 4578 4725 5136 3906 Size: 398 274 147 411 4243 Fragments arranged by size: 4243 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5248 Fragments arranged by size: 5248 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2006 1186 Size: 820 4653 BseYI C'CCAG_C Cuts at: 2100 2430 2465 4676 5172 2100 Size: 330 35 2211 496 2401 Fragments arranged by size: 2401 2211 496 330 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2607 1163 Size: 573 871 4029 Fragments arranged by size: 4029 871 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2923 3684 4712 1540 Size: 275 186 922 761 1028 2301 Fragments arranged by size: 2301 1028 922 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2092 2229 3534 3619 4690 2092 Size: 137 1305 85 1071 2875 Fragments arranged by size: 2875 1305 1071 137 85 BsmI GAATG_Cn' Cuts at: 2984 3077 2984 Size: 93 5380 BsmBI CGTCTCn'nnnn_ Cuts at: 2277 2277 Size: 5473 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2092 2229 2474 2792 3534 3619 4690 Size: 399 302 137 245 318 742 85 1071 Cuts at: 4690 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 318 302 245 137 85 BspEI T'CCGG_A Cuts at: 5136 5136 Size: 5473 BspHI T'CATG_A Cuts at: 2205 3362 5092 2205 Size: 1157 1730 2586 Fragments arranged by size: 2586 1730 1157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2241 2828 844 Size: 1397 587 3489 Fragments arranged by size: 3489 1397 587 BsrBI CCG'CTC Cuts at: 1394 1823 3360 1394 Size: 429 1537 3507 Fragments arranged by size: 3507 1537 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3968 4142 65 Size: 1183 2720 174 1396 Fragments arranged by size: 2720 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4117 4304 1700 Size: 110 2307 187 2869 Fragments arranged by size: 2869 2307 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5473 BssHII G'CGCG_C Cuts at: 1925 2303 1925 Size: 378 5095 BssSI C'ACGA_G Cuts at: 3533 4545 3533 Size: 1012 4461 BstAPI GCAn_nnn'nTGC Cuts at: 794 2233 794 Size: 1439 4034 BstBI TT'CG_AA Cuts at: 4277 4277 Size: 5473 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5473 BstYI r'GATC_y Cuts at: 1972 2803 3170 3555 3572 5024 5110 5122 5468 Size: 831 367 385 17 1452 86 12 346 Cuts at: 5468 1972 Size: 1977 Fragments arranged by size: 1977 1452 831 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2105 2376 2577 513 Size: 552 1040 271 201 3409 Fragments arranged by size: 3409 1040 552 271 201 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2259 487 Size: 683 56 1033 3701 Fragments arranged by size: 3701 1033 683 56 BtsI GCAGTG_nn' Cuts at: 2188 2989 3783 3803 2188 Size: 801 794 20 3858 Fragments arranged by size: 3858 801 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5473 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5438 DraI TTT'AAA Cuts at: 2777 2777 Size: 5473 DraIII CAC_nnn'GTG Cuts at: 2173 2173 Size: 5473 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4480 809 Size: 507 3164 1802 Fragments arranged by size: 3164 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2920 3809 5438 Size: 54 1425 50 275 18 1090 889 1629 Cuts at: 5438 8 Size: 43 Fragments arranged by size: 1629 1425 1090 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2920 1537 Size: 275 1108 4090 Fragments arranged by size: 4090 1108 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2759 3403 1353 Size: 1406 644 3423 Fragments arranged by size: 3423 1406 644 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4060 4434 4580 387 Size: 1064 138 2471 374 146 1280 Fragments arranged by size: 2471 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3536 4133 4920 5188 1239 Size: 393 1904 597 787 268 1524 Fragments arranged by size: 1904 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2434 2636 2434 Size: 202 5271 EcoRI G'AATT_C Cuts at: 2782 3160 2782 Size: 378 5095 FspI TGC'GCA Cuts at: 2237 3979 2237 Size: 1742 3731 HaeII r_GCGC'y Cuts at: 1678 2151 2566 4620 1678 Size: 473 415 2054 2531 Fragments arranged by size: 2531 2054 473 415 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2257 2289 2628 2660 4114 Size: 32 135 32 685 32 339 32 1454 Cuts at: 4114 4146 4188 4220 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1454 685 339 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2817 3063 669 Size: 988 281 879 246 3079 Fragments arranged by size: 3079 988 879 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5473 HpaI GTT'AAC Cuts at: 3063 3063 Size: 5473 KasI G'GCGC_C Cuts at: 2562 2562 Size: 5473 KpnI G_GTAC'C Cuts at: 2798 2798 Size: 5473 MfeI C'AATT_G Cuts at: 3072 3072 Size: 5473 MluI A'CGCG_T Cuts at: 2390 5349 2390 Size: 2959 2514 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4587 4771 1305 Size: 3282 184 2007 Fragments arranged by size: 3282 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3651 Fragments arranged by size: 3651 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 2579 3431 3790 3949 518 Size: 648 1044 369 852 359 159 2042 Fragments arranged by size: 2042 1044 852 648 369 359 159 MspA1I CmG'CkG Cuts at: 1606 2378 2465 2889 3566 4714 4959 1606 Size: 772 87 424 677 1148 245 2120 Fragments arranged by size: 2120 1148 772 677 424 245 87 NarI GG'CG_CC Cuts at: 2563 2563 Size: 5473 NcoI C'CATG_G Cuts at: 513 1065 2105 2577 513 Size: 552 1040 472 3409 Fragments arranged by size: 3409 1040 552 472 NdeI CA'TA_TG Cuts at: 387 387 Size: 5473 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5473 NotI GC'GGCC_GC Cuts at: 2920 2920 Size: 5473 NspI r_CATG'y Cuts at: 1132 1229 2831 4376 1132 Size: 97 1602 1545 2229 Fragments arranged by size: 2229 1602 1545 97 PasI CC'CwG_GG Cuts at: 1348 2558 1348 Size: 1210 4263 PciI A'CATG_T Cuts at: 4372 4372 Size: 5473 PflMI CCAn_nnn'nTGG Cuts at: 1108 2740 1108 Size: 1632 3841 PmeI GTTT'AAAC Cuts at: 2777 2777 Size: 5473 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3542 3574 5055 5087 3542 Size: 32 1481 32 3928 Fragments arranged by size: 3928 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2636 2636 Size: 5473 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5473 PsiI TTA'TAA Cuts at: 3043 3043 Size: 5473 PstI C_TGCA'G Cuts at: 830 2664 2825 830 Size: 1834 161 3478 Fragments arranged by size: 3478 1834 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5473 SacII CC_GC'GG Cuts at: 2379 2379 Size: 5473 SalI G'TCGA_C Cuts at: 1936 2815 1936 Size: 879 4594 SanDI GG'GwC_CC Cuts at: 2636 2636 Size: 5473 SbfI CC_TGCA'GG Cuts at: 2664 2825 2664 Size: 161 5312 ScaI AGT'ACT Cuts at: 3721 5433 3721 Size: 1712 3761 SfcI C'TryA_G Cuts at: 826 1045 2660 2821 3956 4637 4828 826 Size: 219 1615 161 1135 681 191 1471 Fragments arranged by size: 1615 1471 1135 681 219 191 161 SfoI GGC'GCC Cuts at: 2564 2564 Size: 5473 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5473 SmaI CCC'GGG Cuts at: 1872 2406 2800 1872 Size: 534 394 4545 Fragments arranged by size: 4545 534 394 SmlI C'TyrA_G Cuts at: 820 1017 1943 3577 4478 4740 820 Size: 197 926 1634 901 262 1553 Fragments arranged by size: 1634 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3264 493 Size: 2771 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5473 SphI G_CATG'C Cuts at: 1132 2831 1132 Size: 1699 3774 SspI AAT'ATT Cuts at: 5 52 3397 5 Size: 47 3345 2081 Fragments arranged by size: 3345 2081 47 StuI AGG'CCT Cuts at: 1517 2414 1517 Size: 897 4576 StyI C'CwwG_G Cuts at: 513 1065 2105 2577 2912 513 Size: 552 1040 472 335 3074 Fragments arranged by size: 3074 1040 552 472 335 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2161 3500 3517 3670 3855 4300 325 Size: 1255 581 1339 17 153 185 445 1498 Fragments arranged by size: 1498 1339 1255 581 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3719 5431 96 Size: 274 80 33 51 1443 1742 1712 138 Fragments arranged by size: 1742 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5433 5465 5433 Size: 32 5441 Tth111I GACn'n_nGTC Cuts at: 1316 2269 1316 Size: 953 4520 XbaI T'CTAG_A Cuts at: 2809 2809 Size: 5473 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2453 2453 Size: 5473 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5473 XmaI C'CCGG_G Cuts at: 1870 2404 2798 1870 Size: 534 394 4545 Fragments arranged by size: 4545 534 394 XmnI GAAnn'nnTTC Cuts at: 3602 3602 Size: 5473 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2686 3281 276 Size: 53 83 186 936 1152 595 2468 Fragments arranged by size: 2468 1152 936 595 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI