################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspHI BsrBI BsrGI BssHII Bst1107I BstUI BstXI BstZ17I CelII CfoI ClaI CpoI DraII DraIII EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I KasI KpnI KspI MaeII MamI MluI MluNI MscI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 2142 GTCTAC GT^[AC][GT]AC AccIII 1125 TCCGGA T^CCGGA AciI 2300 CCGC CCGC(-3/-1) AcyI 4 GACGCC G[GA]^CG[CT]C AhdI 2134 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 1306 ATTAAT AT^TAAT AsnI 1306 ATTAAT AT^TAAT AspEI 2134 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 2354 GAGCTC G[AT]GC[AT]^C AspI 2242 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 94 GGCACC G^G[CT][GA]CC BanII 2354 GAGCTC G[GA]GC[CT]^C BbvCI 1143 CCTCAGC CCTCAGC(-5/-2) BglII 366 AGATCT A^GATCT BsaHI 4 GACGCC G[GA]^CG[CT]C BseAI 1125 TCCGGA T^CCGGA BseRI 808 CTCCTC GAGGAG(10/8) BsgI 481 GTGCAG GTGCAG(16/14) BsiHKAI 2354 GAGCTC G[AT]GC[AT]^C BsmFI 2132 GGGAC GGGAC(10/14) BsmI 420 GAATGC GAATGC(1/-1) BspEI 1125 TCCGGA T^CCGGA BsrFI 1963 ACCGGC [GA]^CCGG[CT] BssSI 2333 CTCGTG CACGAG(-5/-1) BstAPI 615 GCAACCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1253 TTCGAA TT^CGAA Cfr10I 1963 ACCGGC [GA]^CCGG[CT] DrdI 820 GACAGTGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 2128 CCATGG C^C[GA][CT]GG Eam1105I 2134 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Hin1I 4 GACGCC G[GA]^CG[CT]C HpaI 963 GTTAAC GTT^AAC MfeI 2192 CAATTG C^AATTG MroI 1125 TCCGGA T^CCGGA MslI 846 CATAACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1439 CAGCTG C[AC]G^C[GT]G MunI 2192 CAATTG C^AATTG NcoI 2128 CCATGG C^CATGG NlaIV 94 GGCACC GG[ACGT]^[ACGT]CC NspV 1253 TTCGAA TT^CGAA PflFI 2242 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 2242 GACTC GAGTC(4/5) PshBI 1306 ATTAAT AT^TAAT PvuII 1439 CAGCTG CAG^CTG SacI 2354 GAGCTC GAGCT^C SbfI 2269 CCTGCAGG CCTGCA^GG SfuI 1253 TTCGAA TT^CGAA Sse8387I 2269 CCTGCAGG CCTGCA^GG SspI 773 AATATT AAT^ATT StuI 2322 AGGCCT AGG^CCT Tth111I 2242 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflIII 1118 ACATGT A^C[GA][CT]GT 2031 ACATGT AlwNI 620 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 2562 CAGAGACTG AocI 1026 CCTCAGG CC^T[ACGT]AGG 1351 CCTCAGG AvaII 1339 GGTCC G^G[AT]CC 2581 GGACC BciVI 19 GGATAC GTATCC(6/5) 2513 GTATCC BmyI 232 GTGCCC G[AGT]GC[ACT]^C 2354 GAGCTC Bsp1286I 232 GTGCCC G[AGT]GC[ACT]^C 2354 GAGCTC BspLU11I 1118 ACATGT A^CATGT 2031 ACATGT BstEII 442 GGTGACC G^GT[ACGT]ACC 1803 GGTTACC BstPI 442 GGTGACC G^GT[ACGT]ACC 1803 GGTTACC BstYI 366 AGATCT [GA]^GATC[CT] 526 GGATCT Bsu36I 1026 CCTCAGG CC^T[ACGT]AGG 1351 CCTCAGG EarI 811 CTCTTC CTCTTC(1/4) 2360 GAAGAG Eco81I 1026 CCTCAGG CC^T[ACGT]AGG 1351 CCTCAGG EcoO65I 442 GGTGACC G^GT[ACGT]ACC 1803 GGTTACC EcoT14I 2128 CCATGG C^C[AT][AT]GG 2702 CCTTGG Ksp632I 811 CTCTTC CTCTTC(1/4) 2360 GAAGAG MflI 366 AGATCT [GA]^GATC[CT] 526 GGATCT NspI 1118 ACATGT [GA]CATG^[CT] 2031 ACATGT SfaNI 2156 GATGC GCATC(5/9) 2371 GATGC StyI 2128 CCATGG C^C[AT][AT]GG 2702 CCTTGG XhoII 366 AGATCT [GA]^GATC[CT] 526 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI Asp700I BbvI BfaI BpmI BsaJI BsaWI BsiYI BslI BsmAI BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI EcoRI EcoRII Fnu4HI FokI HaeIII HapII HgaI HincII HindII HindIII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII PflMI PstI RsaI Sau3AI Sau96I ScrFI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XmnI