(Circular) (Six-base) MAPSORT of: pFN21AB8529.seq Check: 3504 from: 1 to: 7594 pFN21AB8529 7594 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4282 4282 Size: 7594 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5404 278 Size: 53 83 186 936 3868 2468 Fragments arranged by size: 3868 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4148 4937 1937 Size: 2211 789 4594 Fragments arranged by size: 4594 2211 789 Acc65I G'GTAC_C Cuts at: 4915 4915 Size: 7594 AclI AA'CG_TT Cuts at: 1563 5721 6094 1563 Size: 4158 373 3063 Fragments arranged by size: 4158 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2389 2675 3261 4661 4770 5657 7041 7309 Size: 757 286 586 1400 109 887 1384 268 Cuts at: 7309 1632 Size: 1917 Fragments arranged by size: 1917 1400 1384 887 757 586 286 268 109 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7397 AflIII A'CryG_T Cuts at: 1203 3123 4036 6493 7470 1203 Size: 1920 913 2457 977 1327 Fragments arranged by size: 2457 1920 1327 977 913 AgeI A'CCGG_T Cuts at: 6425 6425 Size: 7594 AhdI GACnn_n'nnGTC Cuts at: 4144 6323 4144 Size: 2179 5415 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7594 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7594 AlwNI CAG_nnn'CTG Cuts at: 2630 4572 5337 6909 2630 Size: 1942 765 1572 3315 Fragments arranged by size: 3315 1942 1572 765 ApaLI G'TGCA_C Cuts at: 5651 6807 5651 Size: 1156 6438 ApoI r'AATT_y Cuts at: 1493 2666 3271 3319 3819 3838 4099 4343 4886 Size: 1173 605 48 500 19 261 244 543 Cuts at: 4886 4903 5129 5281 7390 1493 Size: 17 226 152 2109 1697 Fragments arranged by size: 2109 1697 1173 605 543 500 261 244 226 152 48 19 17 AseI AT'TA_AT Cuts at: 160 1032 3312 6148 160 Size: 872 2280 2836 1606 Fragments arranged by size: 2836 2280 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7594 AvaI C'yCGr_G Cuts at: 1870 1943 4919 1870 Size: 73 2976 4545 Fragments arranged by size: 4545 2976 73 BamHI G'GATC_C Cuts at: 4924 5291 4924 Size: 367 7227 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2099 4915 6370 618 Size: 325 217 627 312 2816 1455 1842 Fragments arranged by size: 2816 1842 1455 627 325 312 217 BanII G_rGCy'C Cuts at: 1391 4363 4913 1391 Size: 2972 550 4072 Fragments arranged by size: 4072 2972 550 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6623 BbvCI CC'TCA_GC Cuts at: 3149 3149 Size: 7594 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5785 5819 1903 Size: 34 3848 34 3678 Fragments arranged by size: 3848 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2018 4529 5486 6696 2018 Size: 2511 957 1210 2916 Fragments arranged by size: 2916 2511 1210 957 BclI T'GATC_A Cuts at: 1548 1548 Size: 7594 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6205 136 Size: 107 122 71 631 605 4533 1525 Fragments arranged by size: 4533 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2371 7589 2371 Size: 5218 2376 BlpI GC'TnA_GC Cuts at: 5011 5011 Size: 7594 Bme1580I G_kGCm'C Cuts at: 1790 2241 5655 6811 1790 Size: 451 3414 1156 2573 Fragments arranged by size: 3414 2573 1156 451 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7594 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2982 4582 4607 Size: 416 53 83 399 24 1565 1600 25 Cuts at: 4607 5325 6283 442 Size: 718 958 1753 Fragments arranged by size: 1753 1600 1565 958 718 416 399 83 53 25 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7594 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2460 2565 3630 4190 4549 6254 1239 Size: 1221 105 1065 560 359 1705 2579 Fragments arranged by size: 2579 1705 1221 1065 560 359 105 Bpu10I CC'TnA_GC Cuts at: 3149 3149 Size: 7594 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5719 6584 6882 5719 Size: 865 298 6431 Fragments arranged by size: 6431 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6257 882 Size: 617 4758 2219 Fragments arranged by size: 4758 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5385 493 Size: 711 4181 2702 Fragments arranged by size: 4181 2702 711 BsaBI GATnn'nnATC Cuts at: 7588 7588 Size: 7594 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 5401 5783 6418 Size: 53 83 186 936 477 3391 382 635 Cuts at: 6418 275 Size: 1451 Fragments arranged by size: 3391 1451 936 635 477 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3130 4114 4718 6027 6425 6699 6846 7257 3130 Size: 984 604 1309 398 274 147 411 3467 Fragments arranged by size: 3467 1309 984 604 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4236 4266 1178 Size: 30 165 30 2833 30 4506 Fragments arranged by size: 4506 2833 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2804 1186 Size: 1618 5976 BseYI C'CCAG_C Cuts at: 6797 7293 6797 Size: 496 7098 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2507 1163 Size: 573 771 6250 Fragments arranged by size: 6250 771 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5044 5805 6833 1540 Size: 275 186 3043 761 1028 2301 Fragments arranged by size: 3043 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4363 5655 5740 6811 4363 Size: 1292 85 1071 5146 Fragments arranged by size: 5146 1292 1071 85 BsmI GAATG_Cn' Cuts at: 2431 5105 5198 2431 Size: 2674 93 4827 Fragments arranged by size: 4827 2674 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2241 4363 4913 5655 5740 6811 1391 Size: 399 451 2122 550 742 85 1071 2174 Fragments arranged by size: 2174 2122 1071 742 550 451 399 85 BspEI T'CCGG_A Cuts at: 3130 7257 3130 Size: 4127 3467 BspHI T'CATG_A Cuts at: 5483 7213 5483 Size: 1730 5864 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2460 2531 3566 3993 4282 4949 844 Size: 1616 71 1035 427 289 667 3489 Fragments arranged by size: 3489 1616 1035 667 427 289 71 BsrBI CCG'CTC Cuts at: 1394 1823 5481 1394 Size: 429 3658 3507 Fragments arranged by size: 3658 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2233 2295 3519 6089 6263 65 Size: 1183 985 62 1224 2570 174 1396 Fragments arranged by size: 2570 1396 1224 1183 985 174 62 BsrFI r'CCGG_y Cuts at: 1700 1810 3968 6238 6425 1700 Size: 110 2158 2270 187 2869 Fragments arranged by size: 2869 2270 2158 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7594 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7594 BssSI C'ACGA_G Cuts at: 4338 5654 6666 4338 Size: 1316 1012 5266 Fragments arranged by size: 5266 1316 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2626 794 Size: 1832 5762 BstBI TT'CG_AA Cuts at: 3259 6398 3259 Size: 3139 4455 BstEII G'GTnAC_C Cuts at: 1184 2447 3808 1184 Size: 1263 1361 4970 Fragments arranged by size: 4970 1361 1263 BstYI r'GATC_y Cuts at: 1972 2371 2531 4924 5291 5676 5693 7145 7231 Size: 399 160 2393 367 385 17 1452 86 Cuts at: 7231 7243 7589 1972 Size: 12 346 1977 Fragments arranged by size: 2393 1977 1452 399 385 367 346 160 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3032 3357 3032 Size: 325 7269 BtgI C'CryG_G Cuts at: 513 1065 2004 4133 513 Size: 552 939 2129 3974 Fragments arranged by size: 3974 2129 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6855 Fragments arranged by size: 6855 683 56 BtsI GCAGTG_nn' Cuts at: 2325 2739 3658 5110 5904 5924 2325 Size: 414 919 1452 794 20 3995 Fragments arranged by size: 3995 1452 919 794 414 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7594 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3729 3764 529 Size: 35 3165 35 4359 Fragments arranged by size: 4359 3165 35 35 DraI TTT'AAA Cuts at: 3427 3783 4057 4898 3427 Size: 356 274 841 6123 Fragments arranged by size: 6123 841 356 274 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2831 6601 809 Size: 507 1515 3770 1802 Fragments arranged by size: 3770 1802 1515 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5041 5930 7559 Size: 54 1425 50 275 18 3211 889 1629 Cuts at: 7559 8 Size: 43 Fragments arranged by size: 3211 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5041 1537 Size: 275 3229 4090 Fragments arranged by size: 4090 3229 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2822 4360 5524 1353 Size: 1469 1538 1164 3423 Fragments arranged by size: 3423 1538 1469 1164 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6181 6555 6701 387 Size: 1064 138 4592 374 146 1280 Fragments arranged by size: 4592 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4361 4361 Size: 7594 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2389 2460 2565 2675 3261 3630 4190 Size: 393 757 71 105 110 586 369 560 Cuts at: 4190 4549 4661 4770 5657 6254 7041 7309 1239 Size: 359 112 109 887 597 787 268 1524 Fragments arranged by size: 1524 887 787 757 597 586 560 393 369 359 268 112 110 109 105 71 EcoRI G'AATT_C Cuts at: 2666 3271 3319 4099 4343 4903 5281 2666 Size: 605 48 780 244 560 378 4979 Fragments arranged by size: 4979 780 605 560 378 244 48 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2095 3869 4413 2095 Size: 1774 544 5276 Fragments arranged by size: 5276 1774 544 FspI TGC'GCA Cuts at: 6100 6100 Size: 7594 HaeII r_GCGC'y Cuts at: 1678 6741 1678 Size: 5063 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3452 3484 3501 3533 3705 Size: 32 135 32 1880 32 17 32 172 Cuts at: 3705 3737 4130 4162 4236 4253 4268 4285 6235 Size: 32 393 32 74 17 15 17 1950 Cuts at: 6235 6267 6309 6341 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1950 1880 393 172 135 74 42 32 32 32 32 32 32 32 32 17 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 2383 2970 3166 4938 5184 669 Size: 988 281 445 587 196 1772 246 3079 Fragments arranged by size: 3079 1772 988 587 445 281 246 196 HindIII A'AGCT_T Cuts at: 748 2498 3707 4412 748 Size: 1750 1209 705 3930 Fragments arranged by size: 3930 1750 1209 705 HpaI GTT'AAC Cuts at: 2970 5184 2970 Size: 2214 5380 KpnI G_GTAC'C Cuts at: 4919 4919 Size: 7594 MfeI C'AATT_G Cuts at: 4197 5193 4197 Size: 996 6598 MluI A'CGCG_T Cuts at: 7470 7470 Size: 7594 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2121 2390 2872 2938 3400 6708 6892 1305 Size: 816 269 482 66 462 3308 184 2007 Fragments arranged by size: 3308 2007 816 482 462 269 184 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5772 Fragments arranged by size: 5772 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2855 5552 5911 6070 518 Size: 648 1689 2697 359 159 2042 Fragments arranged by size: 2697 2042 1689 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3446 5010 5687 6835 7080 1606 Size: 1840 1564 677 1148 245 2120 Fragments arranged by size: 2120 1840 1564 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 4133 513 Size: 552 939 2129 3974 Fragments arranged by size: 3974 2129 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7594 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7594 NotI GC'GGCC_GC Cuts at: 5041 5041 Size: 7594 NspI r_CATG'y Cuts at: 1132 1229 3127 4040 4952 6497 1132 Size: 97 1898 913 912 1545 2229 Fragments arranged by size: 2229 1898 1545 913 912 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7594 PciI A'CATG_T Cuts at: 3123 4036 6493 3123 Size: 913 2457 4224 Fragments arranged by size: 4224 2457 913 PflMI CCAn_nnn'nTGG Cuts at: 1108 2438 3653 4572 1108 Size: 1330 1215 919 4130 Fragments arranged by size: 4130 1330 1215 919 PfoI T'CCnGG_A Cuts at: 2437 2743 4687 4762 2437 Size: 306 1944 75 5269 Fragments arranged by size: 5269 1944 306 75 PmeI GTTT'AAAC Cuts at: 4898 4898 Size: 7594 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3999 4031 5663 5695 7176 7208 3999 Size: 32 1632 32 1481 32 4385 Fragments arranged by size: 4385 1632 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7594 PsiI TTA'TAA Cuts at: 5164 5164 Size: 7594 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4026 4058 4026 Size: 32 7562 PstI C_TGCA'G Cuts at: 830 2457 2510 3529 3577 4279 4498 4946 830 Size: 1627 53 1019 48 702 219 448 3478 Fragments arranged by size: 3478 1627 1019 702 448 219 53 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7594 PvuII CAG'CTG Cuts at: 3446 3446 Size: 7594 SacI G_AGCT'C Cuts at: 4363 4363 Size: 7594 SalI G'TCGA_C Cuts at: 1936 4936 1936 Size: 3000 4594 SbfI CC_TGCA'GG Cuts at: 4279 4946 4279 Size: 667 6927 ScaI AGT'ACT Cuts at: 5842 7554 5842 Size: 1712 5882 SfcI C'TryA_G Cuts at: 826 1045 2453 2506 3096 3525 3573 4275 4494 Size: 219 1408 53 590 429 48 702 219 Cuts at: 4494 4942 6077 6758 6949 826 Size: 448 1135 681 191 1471 Fragments arranged by size: 1471 1408 1135 702 681 590 448 429 219 219 191 53 48 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7594 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7594 SmaI CCC'GGG Cuts at: 1872 4921 1872 Size: 3049 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5698 6599 6861 820 Size: 197 926 3755 901 262 1553 Fragments arranged by size: 3755 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5385 493 Size: 4892 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7594 SphI G_CATG'C Cuts at: 1132 4952 1132 Size: 3820 3774 SspI AAT'ATT Cuts at: 5 52 2780 5518 5 Size: 47 2728 2738 2081 Fragments arranged by size: 2738 2728 2081 47 StuI AGG'CCT Cuts at: 1517 4329 1517 Size: 2812 4782 StyI C'CwwG_G Cuts at: 513 1065 2004 4133 4707 5033 513 Size: 552 939 2129 574 326 3074 Fragments arranged by size: 3074 2129 939 574 552 326 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2611 4589 5621 5638 5791 5976 6421 Size: 1255 1031 1978 1032 17 153 185 445 Cuts at: 6421 325 Size: 1498 Fragments arranged by size: 1978 1498 1255 1032 1031 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5840 7552 96 Size: 274 80 33 51 1443 3863 1712 138 Fragments arranged by size: 3863 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4665 4697 7554 7586 4665 Size: 32 2857 32 4673 Fragments arranged by size: 4673 2857 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4250 1316 Size: 2934 4660 XbaI T'CTAG_A Cuts at: 4930 4930 Size: 7594 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7594 XmaI C'CCGG_G Cuts at: 1870 4919 1870 Size: 3049 4545 XmnI GAAnn'nnTTC Cuts at: 2078 2572 4014 5723 2078 Size: 494 1442 1709 3949 Fragments arranged by size: 3949 1709 1442 494 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5402 276 Size: 53 83 186 936 3868 2468 Fragments arranged by size: 3868 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI