(Linear) (Six-base) MAPSORT of: KIBB8529.seq Check: 7183 from: 1 to: 2889 KIBB8529.orf With 182 enzymes: SgfI * December 5, 2014 12:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2277 2889 Size: 2277 612 AccI GT'mk_AC Cuts at: 0 2143 2889 Size: 2143 746 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 384 670 1256 2656 2765 2889 Size: 384 286 586 1400 109 124 Fragments arranged by size: 1400 586 384 286 124 109 AflIII A'CryG_T Cuts at: 0 1118 2031 2889 Size: 1118 913 858 Fragments arranged by size: 1118 913 858 AhdI GACnn_n'nnGTC Cuts at: 0 2139 2889 Size: 2139 750 AlwNI CAG_nnn'CTG Cuts at: 0 625 2567 2889 Size: 625 1942 322 Fragments arranged by size: 1942 625 322 ApoI r'AATT_y Cuts at: 0 661 1266 1314 1814 1833 2094 2338 2881 Size: 661 605 48 500 19 261 244 543 Cuts at: 2881 2889 Size: 8 Fragments arranged by size: 661 605 543 500 261 244 48 19 8 AseI AT'TA_AT Cuts at: 0 1307 2889 Size: 1307 1582 BanI G'GyrC_C Cuts at: 0 94 2889 Size: 94 2795 BanII G_rGCy'C Cuts at: 0 2358 2889 Size: 2358 531 BbvCI CC'TCA_GC Cuts at: 0 1144 2889 Size: 1144 1745 BciVI GTATCCnnnnn_n' Cuts at: 0 13 2524 2889 Size: 13 2511 365 Fragments arranged by size: 2511 365 13 BglII A'GATC_T Cuts at: 0 366 2889 Size: 366 2523 Bme1580I G_kGCm'C Cuts at: 0 236 2889 Size: 236 2653 BmrI ACTGGGnnnn_n' Cuts at: 0 977 2577 2602 2889 Size: 977 1600 25 287 Fragments arranged by size: 1600 977 287 25 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 455 560 1625 2185 2544 2889 Size: 455 105 1065 560 359 345 Fragments arranged by size: 1065 560 455 359 345 105 Bpu10I CC'TnA_GC Cuts at: 0 1144 2889 Size: 1144 1745 BsaHI Gr'CG_yC Cuts at: 0 5 2889 Size: 5 2884 BsaWI w'CCGG_w Cuts at: 0 1125 2109 2713 2889 Size: 1125 984 604 176 Fragments arranged by size: 1125 984 604 176 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2231 2261 2889 Size: 2231 30 628 Fragments arranged by size: 2231 628 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 799 2889 Size: 799 2090 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 2889 Size: 502 2387 BsiHKAI G_wGCw'C Cuts at: 0 2358 2889 Size: 2358 531 BsmI GAATG_Cn' Cuts at: 0 426 2889 Size: 426 2463 Bsp1286I G_dGCh'C Cuts at: 0 236 2358 2889 Size: 236 2122 531 Fragments arranged by size: 2122 531 236 BspEI T'CCGG_A Cuts at: 0 1125 2889 Size: 1125 1764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 455 526 1561 1988 2277 2889 Size: 455 71 1035 427 289 612 Fragments arranged by size: 1035 612 455 427 289 71 BsrDI GCAATG_nn' Cuts at: 0 228 290 1514 2889 Size: 228 62 1224 1375 Fragments arranged by size: 1375 1224 228 62 BsrFI r'CCGG_y Cuts at: 0 1963 2889 Size: 1963 926 BssSI C'ACGA_G Cuts at: 0 2333 2889 Size: 2333 556 BstAPI GCAn_nnn'nTGC Cuts at: 0 621 2889 Size: 621 2268 BstBI TT'CG_AA Cuts at: 0 1254 2889 Size: 1254 1635 BstEII G'GTnAC_C Cuts at: 0 442 1803 2889 Size: 442 1361 1086 Fragments arranged by size: 1361 1086 442 BstYI r'GATC_y Cuts at: 0 366 526 2889 Size: 366 160 2363 Fragments arranged by size: 2363 366 160 Bsu36I CC'TnA_GG Cuts at: 0 1027 1352 2889 Size: 1027 325 1537 Fragments arranged by size: 1537 1027 325 BtgI C'CryG_G Cuts at: 0 2128 2889 Size: 2128 761 BtsI GCAGTG_nn' Cuts at: 0 320 734 1653 2889 Size: 320 414 919 1236 Fragments arranged by size: 1236 919 414 320 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1724 1759 2889 Size: 1724 35 1130 Fragments arranged by size: 1724 1130 35 DraI TTT'AAA Cuts at: 0 1422 1778 2052 2889 Size: 1422 356 274 837 Fragments arranged by size: 1422 837 356 274 DrdI GACnn_nn'nnGTC Cuts at: 0 826 2889 Size: 826 2063 EarI CTCTTCn'nnn_ Cuts at: 0 817 2355 2889 Size: 817 1538 534 Fragments arranged by size: 1538 817 534 EcoICRI GAG'CTC Cuts at: 0 2356 2889 Size: 2356 533 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 384 455 560 670 1256 1625 2185 2544 Size: 384 71 105 110 586 369 560 359 Cuts at: 2544 2656 2765 2889 Size: 112 109 124 Fragments arranged by size: 586 560 384 369 359 124 112 110 109 105 71 EcoRI G'AATT_C Cuts at: 0 661 1266 1314 2094 2338 2889 Size: 661 605 48 780 244 551 Fragments arranged by size: 780 661 605 551 244 48 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 90 1864 2408 2889 Size: 90 1774 544 481 Fragments arranged by size: 1774 544 481 90 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1447 1479 1496 1528 1700 1732 2125 2157 Size: 1447 32 17 32 172 32 393 32 Cuts at: 2157 2231 2248 2263 2280 2889 Size: 74 17 15 17 609 Fragments arranged by size: 1447 609 393 172 74 32 32 32 32 17 17 17 15 HincII GTy'rAC Cuts at: 0 378 965 1161 2889 Size: 378 587 196 1728 Fragments arranged by size: 1728 587 378 196 HindIII A'AGCT_T Cuts at: 0 493 1702 2407 2889 Size: 493 1209 705 482 Fragments arranged by size: 1209 705 493 482 HpaI GTT'AAC Cuts at: 0 965 2889 Size: 965 1924 MfeI C'AATT_G Cuts at: 0 2192 2889 Size: 2192 697 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 116 385 867 933 1395 2889 Size: 116 269 482 66 462 1494 Fragments arranged by size: 1494 482 462 269 116 66 MslI CAynn'nnrTG Cuts at: 0 850 2889 Size: 850 2039 MspA1I CmG'CkG Cuts at: 0 1441 2889 Size: 1441 1448 NcoI C'CATG_G Cuts at: 0 2128 2889 Size: 2128 761 NspI r_CATG'y Cuts at: 0 1122 2035 2889 Size: 1122 913 854 Fragments arranged by size: 1122 913 854 PciI A'CATG_T Cuts at: 0 1118 2031 2889 Size: 1118 913 858 Fragments arranged by size: 1118 913 858 PflMI CCAn_nnn'nTGG Cuts at: 0 433 1648 2567 2889 Size: 433 1215 919 322 Fragments arranged by size: 1215 919 433 322 PfoI T'CCnGG_A Cuts at: 0 432 738 2682 2757 2889 Size: 432 306 1944 75 132 Fragments arranged by size: 1944 432 306 132 75 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1994 2026 2889 Size: 1994 32 863 Fragments arranged by size: 1994 863 32 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2021 2053 2889 Size: 2021 32 836 Fragments arranged by size: 2021 836 32 PstI C_TGCA'G Cuts at: 0 452 505 1524 1572 2274 2493 2889 Size: 452 53 1019 48 702 219 396 Fragments arranged by size: 1019 702 452 396 219 53 48 PvuII CAG'CTG Cuts at: 0 1441 2889 Size: 1441 1448 SacI G_AGCT'C Cuts at: 0 2358 2889 Size: 2358 531 SbfI CC_TGCA'GG Cuts at: 0 2274 2889 Size: 2274 615 SfcI C'TryA_G Cuts at: 0 448 501 1091 1520 1568 2270 2489 2889 Size: 448 53 590 429 48 702 219 400 Fragments arranged by size: 702 590 448 429 400 219 53 48 SspI AAT'ATT Cuts at: 0 775 2889 Size: 775 2114 StuI AGG'CCT Cuts at: 0 2324 2889 Size: 2324 565 StyI C'CwwG_G Cuts at: 0 2128 2702 2889 Size: 2128 574 187 Fragments arranged by size: 2128 574 187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 606 2584 2889 Size: 606 1978 305 Fragments arranged by size: 1978 606 305 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2660 2692 2889 Size: 2660 32 197 Fragments arranged by size: 2660 197 32 Tth111I GACn'n_nGTC Cuts at: 0 2245 2889 Size: 2245 644 XmnI GAAnn'nnTTC Cuts at: 0 73 567 2009 2889 Size: 73 494 1442 880 Fragments arranged by size: 1442 880 494 73 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AlwNI ApoI AseI BanI BanII BbvCI BciVI BglII Bme1580I BmrI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtsI CspCI DraI DrdI EarI EcoICRI Eco57MI EcoRI FalI Hin4I HincII HindIII HpaI MfeI MmeI MslI MspA1I NcoI NspI PciI PflMI PfoI PpiI PsrI PstI PvuII SacI SbfI SfcI SspI StuI StyI TaqII TstI Tth111I XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI BclI BfrBI BglI BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BseYI BsiEI BsiWI BsmBI BspHI BsrBI BsrGI BssHII BstXI BstZ17I BtgZI ClaI DraIII EaeI EagI EciI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII KasI KpnI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI TatI XbaI XcmI XhoI XmaI ZraI