Restriction map for
pFN21AE6642
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BanII
BbrPI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FseI FspI HapII HgaI HincII
HindII HindIII HpaI HpaII KpnI
KspI MaeI MaeII MamI MfeI
MflI MluI MroI MseI MspI
MunI MvnI NaeI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 686 GAATTC [GA]^AATT[CT]
AcyI 76 GGCGCC G[GA]^CG[CT]C
AfaI 258 GTAC GT^AC
AlwI 263 GGATC GGATC(4/5)
AlwNI 195 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 642 CCTCAGG CC^T[ACGT]AGG
Aor51HI 322 AGCGCT AGC^GCT
ApoI 686 GAATTC [GA]^AATT[CT]
AspI 493 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 781 GGACC G^G[AT]CC
BalI 375 TGGCCA TGG^CCA
BanI 76 GGCGCC G^G[CT][GA]CC
BbeI 76 GGCGCC GGCGC^C
BbsI 577 GAAGAC GAAGAC(2/6)
BbvCI 280 GCTGAGG CCTCAGC(-5/-2)
BbvI 15 GCTGC GCAGC(8/12)
BclI 716 TGATCA T^GATCA
BpmI 171 CTCCAG CTGGAG(16/14)
BpuAI 577 GAAGAC GAAGAC(2/6)
BsaHI 76 GGCGCC G[GA]^CG[CT]C
BsaI 48 GGTCTC GGTCTC(1/5)
BseRI 61 CTCCTC GAGGAG(10/8)
BsmI 240 GAATGC GAATGC(1/-1)
BspMI 563 ACCTGC ACCTGC(4/8)
Bsu36I 642 CCTCAGG CC^T[ACGT]AGG
Cac8I 244 GCCAGC GC[ACGT]^[ACGT]GC
DsaI 488 CCATGG C^C[GA][CT]GG
Eco47III 322 AGCGCT AGC^GCT
Eco81I 642 CCTCAGG CC^T[ACGT]AGG
EcoRI 686 GAATTC G^AATTC
FbaI 716 TGATCA T^GATCA
Hin1I 76 GGCGCC G[GA]^CG[CT]C
HinfI 286 GATTC G^A[ACGT]TC
KasI 76 GGCGCC G^GCGCC
MluNI 375 TGGCCA TGG^CCA
MscI 375 TGGCCA TGG^CCA
MspA1I 776 CAGCTG C[AC]G^C[GT]G
NarI 76 GGCGCC GG^CGCC
NcoI 488 CCATGG C^CATGG
PflFI 493 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 776 CAGCTG CAG^CTG
RsaI 258 GTAC GT^AC
SfoI 76 GGCGCC GGC^GCC
TaqI 126 TCGA T^CGA
TfiI 286 GATTC G^A[AT]TC
TseI 15 GCTGC G^C[AT]GC
Tth111I 493 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 126 TCGA T^CGA
XcmI 451 CCAGACTGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 11 enzymes) #
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BsmAI 128 GAGAC GTCTC(1/5)
49 GTCTC
BsrBI 80 CCGCTC CCGCTC(-3/-3)
359 CCGCTC
BstXI 20 CCAGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
245 CCAGCAACATGG
EaeI 375 TGGCCA [CT]^GGCC[GA]
510 TGGCCG
EcoT14I 94 CCAAGG C^C[AT][AT]GG
488 CCATGG
MwoI 325 GCTCCTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
604 GCCACCAACGC
NlaIII 252 CATG CATG^
489 CATG
PstI 154 CTGCAG CTGCA^G
565 CTGCAG
SfaNI 412 GATGC GCATC(5/9)
483 GATGC
SfcI 154 CTGCAG C^T[GA][CT]AG
565 CTGCAG
StyI 94 CCAAGG C^C[AT][AT]GG
488 CCATGG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
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AciI AluI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI CfoI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MslI MvaI
NdeII NlaIV Sau3AI Sau96I ScrFI
Tsp45I Tsp509I TspRI