(Circular) (Six-base) MAPSORT of: pFN21AE9542.seq Check: 6278 from: 1 to: 6976 pFN21AE9542 6976 bp With 182 enzymes: SgfI * March 28, 2012 12:04 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4786 278 Size: 53 83 186 936 3250 2468 Fragments arranged by size: 3250 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4319 1937 Size: 2382 4594 Acc65I G'GTAC_C Cuts at: 3980 4297 3980 Size: 317 6659 AclI AA'CG_TT Cuts at: 1563 3889 5103 5476 1563 Size: 2326 1214 373 3063 Fragments arranged by size: 3063 2326 1214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2387 2444 2591 2978 3947 5039 6423 6691 Size: 755 57 147 387 969 1092 1384 268 Cuts at: 6691 1632 Size: 1917 Fragments arranged by size: 1917 1384 1092 969 755 387 268 147 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6779 AflIII A'CryG_T Cuts at: 1203 3157 5875 6852 1203 Size: 1954 2718 977 1327 Fragments arranged by size: 2718 1954 1327 977 AgeI A'CCGG_T Cuts at: 5807 5807 Size: 6976 AhdI GACnn_n'nnGTC Cuts at: 2075 5705 2075 Size: 3630 3346 AleI CACnn'nnGTG Cuts at: 1166 2404 1166 Size: 1238 5738 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3277 1184 Size: 2093 4883 AlwNI CAG_nnn'CTG Cuts at: 2137 2467 2893 3845 4719 6291 2137 Size: 330 426 952 874 1572 2822 Fragments arranged by size: 2822 1572 952 874 426 330 ApaLI G'TGCA_C Cuts at: 5033 6189 5033 Size: 1156 5820 ApoI r'AATT_y Cuts at: 1493 2640 2668 2794 3003 3066 3131 3189 3656 Size: 1147 28 126 209 63 65 58 467 Cuts at: 3656 3947 4285 4511 4663 6772 1493 Size: 291 338 226 152 2109 1697 Fragments arranged by size: 2109 1697 1147 467 338 291 226 209 152 126 65 63 58 28 AseI AT'TA_AT Cuts at: 160 1032 2853 3765 5530 160 Size: 872 1821 912 1765 1606 Fragments arranged by size: 1821 1765 1606 912 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6976 AvaI C'yCGr_G Cuts at: 1870 1943 4301 1870 Size: 73 2358 4545 Fragments arranged by size: 4545 2358 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3480 3513 3480 Size: 33 6943 BamHI G'GATC_C Cuts at: 4306 4673 4306 Size: 367 6609 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3677 3980 4297 5752 618 Size: 325 217 627 1890 303 317 1455 1842 Fragments arranged by size: 1890 1842 1455 627 325 317 303 217 BanII G_rGCy'C Cuts at: 1391 3733 4295 1391 Size: 2342 562 4072 Fragments arranged by size: 4072 2342 562 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2014 928 Size: 971 115 5890 Fragments arranged by size: 5890 971 115 BbvCI CC'TCA_GC Cuts at: 2130 2130 Size: 6976 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5167 5201 1903 Size: 34 3230 34 3678 Fragments arranged by size: 3678 3230 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3921 4868 6078 3921 Size: 947 1210 4819 Fragments arranged by size: 4819 1210 947 BclI T'GATC_A Cuts at: 1548 1548 Size: 6976 BfrBI ATG'CAT Cuts at: 3917 3917 Size: 6976 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5587 136 Size: 107 122 71 631 605 3915 1525 Fragments arranged by size: 3915 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2590 6971 2590 Size: 4381 2595 BlpI GC'TnA_GC Cuts at: 4393 4393 Size: 6976 Bme1580I G_kGCm'C Cuts at: 1790 5037 6193 1790 Size: 3247 1156 2573 Fragments arranged by size: 3247 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6976 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4707 5665 442 Size: 416 53 83 399 24 3290 958 1753 Fragments arranged by size: 3290 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6976 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2351 2870 5636 1239 Size: 1112 519 2766 2579 Fragments arranged by size: 2766 2579 1112 519 Bpu10I CC'TnA_GC Cuts at: 2130 2130 Size: 6976 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3023 5101 5966 6264 3023 Size: 2078 865 298 3735 Fragments arranged by size: 3735 2078 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2214 3562 5639 882 Size: 617 715 1348 2077 2219 Fragments arranged by size: 2219 2077 1348 715 617 BsaAI yAC'GTr Cuts at: 493 1204 4767 493 Size: 711 3563 2702 Fragments arranged by size: 3563 2702 711 BsaBI GATnn'nnATC Cuts at: 6970 6970 Size: 6976 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4783 5165 5800 275 Size: 53 83 186 936 3250 382 635 1451 Fragments arranged by size: 3250 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5409 5807 6081 6228 6639 5409 Size: 398 274 147 411 5746 Fragments arranged by size: 5746 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4129 4159 1178 Size: 30 165 30 2726 30 3995 Fragments arranged by size: 3995 2726 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6976 BseYI C'CCAG_C Cuts at: 6179 6675 6179 Size: 496 6480 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 1163 Size: 573 387 6016 Fragments arranged by size: 6016 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4426 5187 6215 1540 Size: 275 186 2425 761 1028 2301 Fragments arranged by size: 2425 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2087 5037 5122 6193 2087 Size: 2950 85 1071 2870 Fragments arranged by size: 2950 2870 1071 85 BsmI GAATG_Cn' Cuts at: 4487 4580 4487 Size: 93 6883 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 3733 4295 5037 5122 6193 1391 Size: 399 297 1646 562 742 85 1071 2174 Fragments arranged by size: 2174 1646 1071 742 562 399 297 85 BspEI T'CCGG_A Cuts at: 6639 6639 Size: 6976 BspHI T'CATG_A Cuts at: 2452 4865 6595 2452 Size: 2413 1730 2833 Fragments arranged by size: 2833 2413 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2481 3269 4331 844 Size: 1637 788 1062 3489 Fragments arranged by size: 3489 1637 1062 788 BsrBI CCG'CTC Cuts at: 1394 1823 4863 1394 Size: 429 3040 3507 Fragments arranged by size: 3507 3040 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2318 2400 5471 5645 65 Size: 1183 1070 82 3071 174 1396 Fragments arranged by size: 3071 1396 1183 1070 174 82 BsrFI r'CCGG_y Cuts at: 1700 1810 5620 5807 1700 Size: 110 3810 187 2869 Fragments arranged by size: 3810 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6976 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6976 BssSI C'ACGA_G Cuts at: 5036 6048 5036 Size: 1012 5964 BstAPI GCAn_nnn'nTGC Cuts at: 794 4186 794 Size: 3392 3584 BstBI TT'CG_AA Cuts at: 5780 5780 Size: 6976 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6976 BstXI CCAn_nnnn'nTGG Cuts at: 3143 3143 Size: 6976 BstYI r'GATC_y Cuts at: 1972 2590 3470 4306 4673 5058 5075 6527 6613 Size: 618 880 836 367 385 17 1452 86 Cuts at: 6613 6625 6971 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 880 836 618 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6424 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6237 Fragments arranged by size: 6237 683 56 BtsI GCAGTG_nn' Cuts at: 3956 4175 4492 5286 5306 3956 Size: 219 317 794 20 5626 Fragments arranged by size: 5626 794 317 219 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6976 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6941 DraI TTT'AAA Cuts at: 4069 4280 4069 Size: 211 6765 DraIII CAC_nnn'GTG Cuts at: 2121 2121 Size: 6976 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2395 5983 809 Size: 507 1079 3588 1802 Fragments arranged by size: 3588 1802 1079 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3477 3771 4172 Size: 54 1425 50 275 18 1647 294 401 Cuts at: 4172 4423 5312 6941 8 Size: 251 889 1629 43 Fragments arranged by size: 1647 1629 1425 889 401 294 275 251 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4423 1537 Size: 275 2611 4090 Fragments arranged by size: 4090 2611 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3705 4906 1353 Size: 2352 1201 3423 Fragments arranged by size: 3423 2352 1201 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5563 5937 6083 387 Size: 1064 138 3974 374 146 1280 Fragments arranged by size: 3974 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2351 2387 2444 2591 2870 2978 3947 Size: 393 719 36 57 147 279 108 969 Cuts at: 3947 5039 5636 6423 6691 1239 Size: 1092 597 787 268 1524 Fragments arranged by size: 1524 1092 969 787 719 597 393 279 268 147 108 57 36 EcoO109I rG'GnC_Cy Cuts at: 2953 2953 Size: 6976 EcoRI G'AATT_C Cuts at: 4285 4663 4285 Size: 378 6598 FspI TGC'GCA Cuts at: 3432 5482 3432 Size: 2050 4926 HaeII r_GCGC'y Cuts at: 1678 6123 1678 Size: 4445 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2061 2093 2914 2946 3222 Size: 32 135 32 489 32 821 32 276 Cuts at: 3222 3254 3285 3317 5617 5649 5691 5723 1373 Size: 32 31 32 2300 32 42 32 2626 Fragments arranged by size: 2626 2300 821 489 276 135 42 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 2735 3513 4320 4566 669 Size: 988 281 797 778 807 246 3079 Fragments arranged by size: 3079 988 807 797 778 281 246 HindIII A'AGCT_T Cuts at: 748 2423 748 Size: 1675 5301 HpaI GTT'AAC Cuts at: 3513 4566 3513 Size: 1053 5923 KpnI G_GTAC'C Cuts at: 3984 4301 3984 Size: 317 6659 MfeI C'AATT_G Cuts at: 2972 4575 2972 Size: 1603 5373 MluI A'CGCG_T Cuts at: 6852 6852 Size: 6976 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3103 6090 6274 1305 Size: 1798 2987 184 2007 Fragments arranged by size: 2987 2007 1798 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3479 4174 10 Size: 54 1425 343 1647 695 2812 Fragments arranged by size: 2812 1647 1425 695 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2064 2284 2404 2862 3287 3828 4160 Size: 648 898 220 120 458 425 541 332 Cuts at: 4160 4934 5293 5452 518 Size: 774 359 159 2042 Fragments arranged by size: 2042 898 774 648 541 458 425 359 332 220 159 120 MspA1I CmG'CkG Cuts at: 1606 3168 3436 4392 5069 6217 6462 1606 Size: 1562 268 956 677 1148 245 2120 Fragments arranged by size: 2120 1562 1148 956 677 268 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6424 NdeI CA'TA_TG Cuts at: 387 4141 387 Size: 3754 3222 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6976 NotI GC'GGCC_GC Cuts at: 4423 4423 Size: 6976 NsiI A_TGCA'T Cuts at: 3919 3919 Size: 6976 NspI r_CATG'y Cuts at: 1132 1229 2743 3161 3292 3431 3811 4334 5879 Size: 97 1514 418 131 139 380 523 1545 Cuts at: 5879 1132 Size: 2229 Fragments arranged by size: 2229 1545 1514 523 418 380 139 131 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6976 PciI A'CATG_T Cuts at: 3157 5875 3157 Size: 2718 4258 PflMI CCAn_nnn'nTGG Cuts at: 1108 2329 2893 1108 Size: 1221 564 5191 Fragments arranged by size: 5191 1221 564 PmeI GTTT'AAAC Cuts at: 4280 4280 Size: 6976 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5045 5077 6558 6590 5045 Size: 32 1481 32 5431 Fragments arranged by size: 5431 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2953 2953 Size: 6976 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6976 PsiI TTA'TAA Cuts at: 4546 4546 Size: 6976 PstI C_TGCA'G Cuts at: 830 2816 3256 4182 4328 830 Size: 1986 440 926 146 3478 Fragments arranged by size: 3478 1986 926 440 146 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6976 PvuII CAG'CTG Cuts at: 3168 3436 3168 Size: 268 6708 SalI G'TCGA_C Cuts at: 1936 4318 1936 Size: 2382 4594 SbfI CC_TGCA'GG Cuts at: 4328 4328 Size: 6976 ScaI AGT'ACT Cuts at: 3140 3451 5224 6936 3140 Size: 311 1773 1712 3180 Fragments arranged by size: 3180 1773 1712 311 SexAI A'CCwGG_T Cuts at: 3976 3976 Size: 6976 SfcI C'TryA_G Cuts at: 826 1045 2812 3252 4099 4178 4324 5459 6140 Size: 219 1767 440 847 79 146 1135 681 Cuts at: 6140 6331 826 Size: 191 1471 Fragments arranged by size: 1767 1471 1135 847 681 440 219 191 146 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6976 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6976 SmaI CCC'GGG Cuts at: 1872 4303 1872 Size: 2431 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3038 5080 5981 6243 820 Size: 197 926 1095 2042 901 262 1553 Fragments arranged by size: 2042 1553 1095 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4767 493 Size: 4274 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6976 SphI G_CATG'C Cuts at: 1132 3292 3431 4334 1132 Size: 2160 139 903 3774 Fragments arranged by size: 3774 2160 903 139 SspI AAT'ATT Cuts at: 5 52 2051 2228 4900 5 Size: 47 1999 177 2672 2081 Fragments arranged by size: 2672 2081 1999 177 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6976 StyI C'CwwG_G Cuts at: 513 1065 2089 2323 4415 513 Size: 552 1024 234 2092 3074 Fragments arranged by size: 3074 2092 1024 552 234 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4150 5003 5020 5173 5358 5803 325 Size: 1255 2570 853 17 153 185 445 1498 Fragments arranged by size: 2570 1498 1255 853 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3138 3449 5222 Size: 274 80 33 51 1443 1161 311 1773 Cuts at: 5222 6934 96 Size: 1712 138 Fragments arranged by size: 1773 1712 1443 1161 311 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2066 2098 6936 6968 2066 Size: 32 4838 32 2074 Fragments arranged by size: 4838 2074 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6976 XbaI T'CTAG_A Cuts at: 3199 4312 3199 Size: 1113 5863 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2751 4155 2751 Size: 1404 5572 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6976 XmaI C'CCGG_G Cuts at: 1870 4301 1870 Size: 2431 4545 XmnI GAAnn'nnTTC Cuts at: 2592 2931 4077 5105 2592 Size: 339 1146 1028 4463 Fragments arranged by size: 4463 1146 1028 339 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4784 276 Size: 53 83 186 936 3250 2468 Fragments arranged by size: 3250 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI