Restriction map for
pFN21AE3351
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AspI AviII BbeI BbrPI BbvCI
BcgI BfrI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstEII BstPI BstUI
Bsu36I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FseI FspI
HaeII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MslI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SfiI SfoI
SgrAI SnaBI SpeI SphI SspBI
SwaI Tth111I XcmI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1916 GTATAC GT^[AC][GT]AC
AcyI 1287 GGCGTC G[GA]^CG[CT]C
ApaI 2993 GGGCCC GGGCC^C
AseI 1449 ATTAAT AT^TAAT
AsnI 1449 ATTAAT AT^TAAT
AvrII 1347 CCTAGG C^CTAGG
BamHI 774 GGATCC G^GATCC
BbsI 1746 GAAGAC GAAGAC(2/6)
BciVI 858 GGATAC GTATCC(6/5)
BclI 2310 TGATCA T^GATCA
BglI 1477 GCCTTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1347 CCTAGG C^CTAGG
BpuAI 1746 GAAGAC GAAGAC(2/6)
BsaHI 1287 GGCGTC G[GA]^CG[CT]C
BseRI 3092 GAGGAG GAGGAG(10/8)
BsrDI 465 GCAATG GCAATG(2/0)
BsrFI 215 ACCGGC [GA]^CCGG[CT]
Bst1107I 1916 GTATAC GTA^TAC
BstBI 515 TTCGAA TT^CGAA
BstXI 1277 CCAATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1916 GTATAC GTA^TAC
CfoI 106 GCGC GCG^C
Cfr10I 215 ACCGGC [GA]^CCGG[CT]
DraI 1425 TTTAAA TTT^AAA
DraIII 184 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1543 CCATGG C^C[GA][CT]GG
FbaI 2310 TGATCA T^GATCA
HgaI 1288 GCGTC GACGC(5/10)
HhaI 106 GCGC GCG^C
Hin1I 1287 GGCGTC G[GA]^CG[CT]C
HinP1I 106 GCGC G^CGC
HindIII 2492 AAGCTT A^AGCTT
NcoI 1543 CCATGG C^CATGG
NdeI 2306 CATATG CA^TATG
NspV 515 TTCGAA TT^CGAA
PaeR7I 2984 CTCGAG C^TCGAG
PpuMI 158 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 1449 ATTAAT AT^TAAT
SbfI 378 CCTGCAGG CCTGCA^GG
SfuI 515 TTCGAA TT^CGAA
SmaI 2990 CCCGGG CCC^GGG
Sse8387I 378 CCTGCAGG CCTGCA^GG
SspI 2207 AATATT AAT^ATT
XbaI 1300 TCTAGA T^CTAGA
XhoI 2984 CTCGAG C^TCGAG
XmaCI 2990 CCCGGG C^CCGGG
XmaI 2990 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1658 AAATTC [GA]^AATT[CT]
2336 AAATTT
AflIII 1357 ACATGT A^C[GA][CT]GT
1883 ACATGT
ApoI 1658 AAATTC [GA]^AATT[CT]
2336 AAATTT
Asp700I 1186 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2387 GAATTACTTC
AspHI 88 GAGCAC G[AT]GC[AT]^C
986 GTGCTC
AvaI 2984 CTCGAG C^[CT]CG[GA]G
2990 CCCGGG
BalI 838 TGGCCA TGG^CCA
1394 TGGCCA
BanI 182 GGCACC G^G[CT][GA]CC
2511 GGTGCC
BanII 2987 GAGCCC G[GA]GC[CT]^C
2993 GGGCCC
BlpI 847 GCTCAGC GC^T[ACGT]AGC
1014 GCTTAGC
Bpu1102I 847 GCTCAGC GC^T[ACGT]AGC
1014 GCTTAGC
BsiHKAI 88 GAGCAC G[AT]GC[AT]^C
986 GTGCTC
BsoBI 2984 CTCGAG C^[CT]CG[GA]G
2990 CCCGGG
BspLU11I 1357 ACATGT A^CATGT
1883 ACATGT
BstAPI 36 GCATCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
183 GCACCAGGTGC
CelII 847 GCTCAGC GC^T[ACGT]AGC
1014 GCTTAGC
EcoNI 264 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
375 CCTCCTGCAGG
EcoT22I 468 ATGCAT ATGCA^T
683 ATGCAT
FauI 54 GCGGG CCCGC(4/6)
564 GCGGG
HincII 503 GTCAAC GT[CT]^[GA]AC
2431 GTTGAC
HindII 503 GTCAAC GT[CT]^[GA]AC
2431 GTTGAC
MaeII 496 ACGT A^CGT
1794 ACGT
MluNI 838 TGGCCA TGG^CCA
1394 TGGCCA
MscI 838 TGGCCA TGG^CCA
1394 TGGCCA
NsiI 468 ATGCAT ATGCA^T
683 ATGCAT
ScaI 1710 AGTACT AGT^ACT
2851 AGTACT
SexAI 185 ACCAGGT A^CC[AT]GGT
981 ACCTGGT
SmlI 49 CTCAAG C^T[CT][GA]AG
2984 CTCGAG
StuI 197 AGGCCT AGG^CCT
1637 AGGCCT
XmnI 1186 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2387 GAATTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BcnI BfaI BglII
BmyI BpmI BsaI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII