Restriction map for
pFN21AE3351
(Circular) (Six-base) MAPSORT of: pFN21AE3351.seq Check: 8216 from: 1 to: 7831
pFN21AE3351 7831 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2170 2170
Size: 7831
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5641 278
Size: 53 83 186 936 4105 2468
Fragments arranged by size:
4105 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3922 5174 1937
Size: 1985 1252 4594
Fragments arranged by size:
4594 1985 1252
Acc65I G'GTAC_C
Cuts at: 5152 5152
Size: 7831
AclI AA'CG_TT
Cuts at: 1563 5958 6331 1563
Size: 4395 373 3063
Fragments arranged by size:
4395 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 2726 3948 4137 4467 5894 7278 7546
Size: 725 369 1222 189 330 1427 1384 268
Cuts at: 7546 1632
Size: 1917
Fragments arranged by size:
1917 1427 1384 1222 725 369 330 268
189
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7634
AflIII A'CryG_T
Cuts at: 1203 3362 3888 6730 7707 1203
Size: 2159 526 2842 977 1327
Fragments arranged by size:
2842 2159 1327 977 526
AgeI A'CCGG_T
Cuts at: 6662 6662
Size: 7831
AhdI GACnn_n'nnGTC
Cuts at: 6560 6560
Size: 7831
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7831
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2451 3495 1184
Size: 1267 1044 5520
Fragments arranged by size:
5520 1267 1044
AlwNI CAG_nnn'CTG
Cuts at: 2197 4160 4490 5574 7146 2197
Size: 1963 330 1084 1572 2882
Fragments arranged by size:
2882 1963 1572 1084 330
ApaI G_GGCC'C
Cuts at: 5002 5002
Size: 7831
ApaLI G'TGCA_C
Cuts at: 5888 7044 5888
Size: 1156 6675
ApoI r'AATT_y
Cuts at: 1493 3663 4341 5140 5366 5518 7627 1493
Size: 2170 678 799 226 152 2109 1697
Fragments arranged by size:
2170 2109 1697 799 678 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3455 6385 160
Size: 872 2423 2930 1606
Fragments arranged by size:
2930 2423 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7831
AvaI C'yCGr_G
Cuts at: 1870 1943 4989 4995 5156 1870
Size: 73 3046 6 161 4545
Fragments arranged by size:
4545 3046 161 73 6
AvrII C'CTAG_G
Cuts at: 3352 3352
Size: 7831
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2856 2889 2856
Size: 33 7798
BamHI G'GATC_C
Cuts at: 2779 5161 5528 2779
Size: 2382 367 5082
Fragments arranged by size:
5082 2382 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2187 4516 5152 6607 618
Size: 325 217 627 400 2329 636 1455 1842
Fragments arranged by size:
2329 1842 1455 636 627 400 325 217
BanII G_rGCy'C
Cuts at: 1391 4996 5002 5150 1391
Size: 3605 6 148 4072
Fragments arranged by size:
4072 3605 148 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3758 928
Size: 971 1859 5001
Fragments arranged by size:
5001 1859 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6022 6056 1903
Size: 34 4085 34 3678
Fragments arranged by size:
4085 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2857 5723 6933 2857
Size: 2866 1210 3755
Fragments arranged by size:
3755 2866 1210
BclI T'GATC_A
Cuts at: 1548 4315 1548
Size: 2767 5064
BfrBI ATG'CAT
Cuts at: 2475 2690 2475
Size: 215 7616
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3488 6442 136
Size: 107 122 71 631 605 1816 2954 1525
Fragments arranged by size:
2954 1816 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3181 3627 3847 7826 3181
Size: 446 220 3979 3186
Fragments arranged by size:
3979 3186 446 220
BlpI GC'TnA_GC
Cuts at: 2853 3020 5248 2853
Size: 167 2228 5436
Fragments arranged by size:
5436 2228 167
Bme1580I G_kGCm'C
Cuts at: 1790 2190 2440 4521 5002 5892 7048 1790
Size: 400 250 2081 481 890 1156 2573
Fragments arranged by size:
2573 2081 1156 890 481 400 250
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7831
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3222 3339 3912
Size: 416 53 83 399 24 1805 117 573
Cuts at: 3912 4686 5562 6520 442
Size: 774 876 958 1753
Fragments arranged by size:
1805 1753 958 876 774 573 416 399
117 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2174 2219 3021 3221 4170 6491 1239
Size: 800 135 45 802 200 949 2321 2579
Fragments arranged by size:
2579 2321 949 802 800 200 135 45
Bpu10I CC'TnA_GC
Cuts at: 3222 3222
Size: 7831
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2039 5956 6821 7119 2039
Size: 3917 865 298 2751
Fragments arranged by size:
3917 2751 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2977 3719 4902 6494 882
Size: 617 1478 742 1183 1592 2219
Fragments arranged by size:
2219 1592 1478 1183 742 617
BsaAI yAC'GTr
Cuts at: 493 1204 5622 493
Size: 711 4418 2702
Fragments arranged by size:
4418 2702 711
BsaBI GATnn'nnATC
Cuts at: 7825 7825
Size: 7831
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3293 5638 6020 6655
Size: 53 83 186 936 1760 2345 382 635
Cuts at: 6655 275
Size: 1451
Fragments arranged by size:
2345 1760 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6264 6662 6936 7083 7494 6264
Size: 398 274 147 411 6601
Fragments arranged by size:
6601 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4672 4702 1178
Size: 30 165 30 3269 30 4307
Fragments arranged by size:
4307 3269 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5112 1186
Size: 3926 3905
BseYI C'CCAG_C
Cuts at: 7034 7530 7034
Size: 496 7335
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2747 4489 4716 1163
Size: 573 1011 1742 227 4278
Fragments arranged by size:
4278 1742 1011 573 227
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5281 6042 7070 1540
Size: 275 186 3280 761 1028 2301
Fragments arranged by size:
3280 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2097 2995 5892 5977 7048 2097
Size: 898 2897 85 1071 2880
Fragments arranged by size:
2897 2880 1071 898 85
BsmI GAATG_Cn'
Cuts at: 3031 3677 4990 5342 5435 3031
Size: 646 1313 352 93 5427
Fragments arranged by size:
5427 1313 646 352 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2190 2440 2995 4521 4996 5002
Size: 399 307 93 250 555 1526 475 6
Cuts at: 5002 5150 5892 5977 7048 1391
Size: 148 742 85 1071 2174
Fragments arranged by size:
2174 1526 1071 742 555 475 399 307
250 148 93 85 6
BspEI T'CCGG_A
Cuts at: 7494 7494
Size: 7831
BspHI T'CATG_A
Cuts at: 5720 7450 5720
Size: 1730 6101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2170 2377 4797 5186 844
Size: 1326 207 2420 389 3489
Fragments arranged by size:
3489 2420 1326 389 207
BsrBI CCG'CTC
Cuts at: 1394 1823 5718 1394
Size: 429 3895 3507
Fragments arranged by size:
3895 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2477 6326 6500 65
Size: 1183 1229 3849 174 1396
Fragments arranged by size:
3849 1396 1229 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2220 6475 6662 1700
Size: 110 410 4255 187 2869
Fragments arranged by size:
4255 2869 410 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7831
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7831
BssSI C'ACGA_G
Cuts at: 5891 6903 5891
Size: 1012 6819
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2047 2194 794
Size: 1253 147 6431
Fragments arranged by size:
6431 1253 147
BstBI TT'CG_AA
Cuts at: 2521 6635 2521
Size: 4114 3717
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7831
BstXI CCAn_nnnn'nTGG
Cuts at: 3289 3289
Size: 7831
BstYI r'GATC_y
Cuts at: 1972 2013 2368 2779 3181 3627 3847 3901 5161
Size: 41 355 411 402 446 220 54 1260
Cuts at: 5161 5528 5913 5930 7382 7468 7480 7826 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1260 446 411 402 385 367
355 346 220 86 54 41 17 12
BstZ17I GTA'TAC
Cuts at: 3923 3923
Size: 7831
BtgI C'CryG_G
Cuts at: 513 1065 2004 3548 513
Size: 552 939 1544 4796
Fragments arranged by size:
4796 1544 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7092
Fragments arranged by size:
7092 683 56
BtsI GCAGTG_nn'
Cuts at: 2120 3682 4358 5347 6141 6161 2120
Size: 1562 676 989 794 20 3790
Fragments arranged by size:
3790 1562 989 794 676 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7831
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4353 4388 529
Size: 35 3789 35 3972
Fragments arranged by size:
3972 3789 35 35
DraI TTT'AAA
Cuts at: 3432 5135 3432
Size: 1703 6128
DraIII CAC_nnn'GTG
Cuts at: 2194 2194
Size: 7831
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6838 809
Size: 507 5522 1802
Fragments arranged by size:
5522 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2222 2843 3399
Size: 54 1425 50 275 18 392 621 556
Cuts at: 3399 5278 6167 7796 8
Size: 1879 889 1629 43
Fragments arranged by size:
1879 1629 1425 889 621 556 392 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5278 1537
Size: 275 3466 4090
Fragments arranged by size:
4090 3466 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2683 2745 3228 3880 4411 4618 5761 1353
Size: 1330 62 483 652 531 207 1143 3423
Fragments arranged by size:
3423 1330 1143 652 531 483 207 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2105 6418 6792 6938 387
Size: 1064 138 516 4313 374 146 1280
Fragments arranged by size:
4313 1280 1064 516 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2174 2219 2357 2726 3021 3221
Size: 393 407 135 45 138 369 295 200
Cuts at: 3221 3948 4137 4170 4467 5894 6491 7278 7546
Size: 727 189 33 297 1427 597 787 268
Cuts at: 7546 1239
Size: 1524
Fragments arranged by size:
1524 1427 787 727 597 407 393 369
297 295 268 200 189 138 135 45
33
EcoNI CCTnn'n_nnAGG
Cuts at: 2273 2384 2273
Size: 111 7720
EcoO109I rG'GnC_Cy
Cuts at: 2023 2164 4999 2023
Size: 141 2835 4855
Fragments arranged by size:
4855 2835 141
EcoRI G'AATT_C
Cuts at: 5140 5518 5140
Size: 378 7453
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2694 4413 2694
Size: 1719 6112
FspI TGC'GCA
Cuts at: 6337 6337
Size: 7831
HaeII r_GCGC'y
Cuts at: 1678 6978 1678
Size: 5300 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2219 2251 2822 2854 3046
Size: 32 135 32 647 32 571 32 192
Cuts at: 3046 3078 3893 3925 4340 4372 4672 4704 6472
Size: 32 815 32 415 32 300 32 1768
Cuts at: 6472 6504 6546 6578 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1768 815 647 571 415 300 192
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2510 4438 5175 5421 669
Size: 988 281 572 1928 737 246 3079
Fragments arranged by size:
3079 1928 988 737 572 281 246
HindIII A'AGCT_T
Cuts at: 748 4497 748
Size: 3749 4082
HpaI GTT'AAC
Cuts at: 5421 5421
Size: 7831
KpnI G_GTAC'C
Cuts at: 5156 5156
Size: 7831
MfeI C'AATT_G
Cuts at: 5430 5430
Size: 7831
MluI A'CGCG_T
Cuts at: 7707 7707
Size: 7831
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4013 6945 7129 1305
Size: 2708 2932 184 2007
Fragments arranged by size:
2932 2708 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2845 3401 10
Size: 54 1425 343 1013 556 4440
Fragments arranged by size:
4440 1425 1013 556 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5789 6148 6307 518
Size: 648 4623 359 159 2042
Fragments arranged by size:
4623 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2176 2515 2900 3998 5247 5924 7072 7317
Size: 570 339 385 1098 1249 677 1148 245
Cuts at: 7317 1606
Size: 2120
Fragments arranged by size:
2120 1249 1148 1098 677 570 385 339
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3548 513
Size: 552 939 1544 4796
Fragments arranged by size:
4796 1544 939 552
NdeI CA'TA_TG
Cuts at: 387 4312 387
Size: 3925 3906
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7831
NotI GC'GGCC_GC
Cuts at: 5278 5278
Size: 7831
NsiI A_TGCA'T
Cuts at: 2477 2692 2477
Size: 215 7616
NspI r_CATG'y
Cuts at: 1132 1229 2861 3016 3366 3892 5189 6734 1132
Size: 97 1632 155 350 526 1297 1545 2229
Fragments arranged by size:
2229 1632 1545 1297 526 350 155 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7831
PciI A'CATG_T
Cuts at: 3362 3888 6730 3362
Size: 526 2842 4463
Fragments arranged by size:
4463 2842 526
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2197 2290 4889 5084 1108
Size: 1089 93 2599 195 3855
Fragments arranged by size:
3855 2599 1089 195 93
PfoI T'CCnGG_A
Cuts at: 2016 2151 2313 2016
Size: 135 162 7534
Fragments arranged by size:
7534 162 135
PmeI GTTT'AAAC
Cuts at: 5135 5135
Size: 7831
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5900 5932 7413 7445 5900
Size: 32 1481 32 6286
Fragments arranged by size:
6286 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 2164
Size: 7831
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7831
PsiI TTA'TAA
Cuts at: 3984 5401 3984
Size: 1417 6414
PspOMI G'GGCC_C
Cuts at: 4998 4998
Size: 7831
PspXI vC'TCGA_Gb
Cuts at: 4989 4989
Size: 7831
PstI C_TGCA'G
Cuts at: 830 2388 2613 3815 5183 830
Size: 1558 225 1202 1368 3478
Fragments arranged by size:
3478 1558 1368 1202 225
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7831
PvuII CAG'CTG
Cuts at: 2515 2900 3998 2515
Size: 385 1098 6348
Fragments arranged by size:
6348 1098 385
SalI G'TCGA_C
Cuts at: 1936 5173 1936
Size: 3237 4594
SbfI CC_TGCA'GG
Cuts at: 2388 5183 2388
Size: 2795 5036
ScaI AGT'ACT
Cuts at: 3717 4858 6079 7791 3717
Size: 1141 1221 1712 3757
Fragments arranged by size:
3757 1712 1221 1141
SexAI A'CCwGG_T
Cuts at: 2190 2986 2190
Size: 796 7035
SfcI C'TryA_G
Cuts at: 826 1045 2384 2609 2706 3811 4428 4919 5179
Size: 219 1339 225 97 1105 617 491 260
Cuts at: 5179 6314 6995 7186 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1339 1135 1105 681 617 491 260
225 219 191 97
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7831
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7831
SmaI CCC'GGG
Cuts at: 1872 4997 5158 1872
Size: 3125 161 4545
Fragments arranged by size:
4545 3125 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 4989 5935 6836 7098 820
Size: 197 926 111 2935 946 901 262 1553
Fragments arranged by size:
2935 1553 946 926 901 262 197 111
SnaBI TAC'GTA
Cuts at: 493 5622 493
Size: 5129 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7831
SphI G_CATG'C
Cuts at: 1132 5189 1132
Size: 4057 3774
SrfI GCCC'GGGC
Cuts at: 4997 4997
Size: 7831
SspI AAT'ATT
Cuts at: 5 52 4214 5755 5
Size: 47 4162 1541 2081
Fragments arranged by size:
4162 2081 1541 47
StuI AGG'CCT
Cuts at: 1517 2204 3644 1517
Size: 687 1440 5704
Fragments arranged by size:
5704 1440 687
StyI C'CwwG_G
Cuts at: 513 1065 2004 2481 3352 3548 5270 513
Size: 552 939 477 871 196 1722 3074
Fragments arranged by size:
3074 1722 939 871 552 477 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2474 2567 3931 5858 5875 6028 6213
Size: 1255 894 93 1364 1927 17 153 185
Cuts at: 6213 6658 325
Size: 445 1498
Fragments arranged by size:
1927 1498 1364 1255 894 445 185 153
93 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3113 3127 3715
Size: 274 80 33 51 1443 1136 14 588
Cuts at: 3715 4604 4856 6077 7789 96
Size: 889 252 1221 1712 138
Fragments arranged by size:
1712 1443 1221 1136 889 588 274 252
138 80 51 33 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2395 2427 7791 7823 2395
Size: 32 5364 32 2403
Fragments arranged by size:
5364 2403 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7831
XbaI T'CTAG_A
Cuts at: 3305 5167 3305
Size: 1862 5969
XhoI C'TCGA_G
Cuts at: 1943 4989 1943
Size: 3046 4785
XmaI C'CCGG_G
Cuts at: 1870 4995 5156 1870
Size: 3125 161 4545
Fragments arranged by size:
4545 3125 161
XmnI GAAnn'nnTTC
Cuts at: 3195 4396 5960 3195
Size: 1201 1564 5066
Fragments arranged by size:
5066 1564 1201
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5639 276
Size: 53 83 186 936 4105 2468
Fragments arranged by size:
4105 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BbvCI BplI BsiWI BsmBI
Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SwaI XcmI