(Linear) (Six-base) MAPSORT of: KIEE3351.seq Check: 5767 from: 1 to: 3126 KIEE3351.orf With 182 enzymes: SgfI * July 3, 2012 17:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 165 3126 Size: 165 2961 AccI GT'mk_AC Cuts at: 0 1917 3126 Size: 1917 1209 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 721 1943 2132 2462 3126 Size: 352 369 1222 189 330 664 Fragments arranged by size: 1222 664 369 352 330 189 AflIII A'CryG_T Cuts at: 0 1357 1883 3126 Size: 1357 526 1243 Fragments arranged by size: 1357 1243 526 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 446 1490 3126 Size: 446 1044 1636 Fragments arranged by size: 1636 1044 446 AlwNI CAG_nnn'CTG Cuts at: 0 192 2155 2485 3126 Size: 192 1963 330 641 Fragments arranged by size: 1963 641 330 192 ApaI G_GGCC'C Cuts at: 0 2997 3126 Size: 2997 129 ApoI r'AATT_y Cuts at: 0 1658 2336 3126 Size: 1658 678 790 Fragments arranged by size: 1658 790 678 AseI AT'TA_AT Cuts at: 0 1450 3126 Size: 1450 1676 AvaI C'yCGr_G Cuts at: 0 2984 2990 3126 Size: 2984 6 136 Fragments arranged by size: 2984 136 6 AvrII C'CTAG_G Cuts at: 0 1347 3126 Size: 1347 1779 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 851 884 3126 Size: 851 33 2242 Fragments arranged by size: 2242 851 33 BamHI G'GATC_C Cuts at: 0 774 3126 Size: 774 2352 BanI G'GyrC_C Cuts at: 0 182 2511 3126 Size: 182 2329 615 Fragments arranged by size: 2329 615 182 BanII G_rGCy'C Cuts at: 0 2991 2997 3126 Size: 2991 6 129 Fragments arranged by size: 2991 129 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 1753 3126 Size: 1753 1373 BciVI GTATCCnnnnn_n' Cuts at: 0 852 3126 Size: 852 2274 BclI T'GATC_A Cuts at: 0 2310 3126 Size: 2310 816 BfrBI ATG'CAT Cuts at: 0 470 685 3126 Size: 470 215 2441 Fragments arranged by size: 2441 470 215 BglI GCCn_nnn'nGGC Cuts at: 0 1483 3126 Size: 1483 1643 BglII A'GATC_T Cuts at: 0 1176 1622 1842 3126 Size: 1176 446 220 1284 Fragments arranged by size: 1284 1176 446 220 BlpI GC'TnA_GC Cuts at: 0 848 1015 3126 Size: 848 167 2111 Fragments arranged by size: 2111 848 167 Bme1580I G_kGCm'C Cuts at: 0 185 435 2516 2997 3126 Size: 185 250 2081 481 129 Fragments arranged by size: 2081 481 250 185 129 BmrI ACTGGGnnnn_n' Cuts at: 0 1217 1334 1907 2681 3126 Size: 1217 117 573 774 445 Fragments arranged by size: 1217 774 573 445 117 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 169 214 1016 1216 2165 3126 Size: 34 135 45 802 200 949 961 Fragments arranged by size: 961 949 802 200 135 45 34 Bpu10I CC'TnA_GC Cuts at: 0 1217 3126 Size: 1217 1909 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 3126 Size: 34 3092 BsaI GGTCTCn'nnnn_ Cuts at: 0 972 1714 2897 3126 Size: 972 742 1183 229 Fragments arranged by size: 1183 972 742 229 BsaHI Gr'CG_yC Cuts at: 0 1288 3126 Size: 1288 1838 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2667 2697 3126 Size: 2667 30 429 Fragments arranged by size: 2667 429 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 3107 3126 Size: 3107 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 742 2484 2711 3126 Size: 742 1742 227 415 Fragments arranged by size: 1742 742 415 227 BsiHKAI G_wGCw'C Cuts at: 0 92 990 3126 Size: 92 898 2136 Fragments arranged by size: 2136 898 92 BsmI GAATG_Cn' Cuts at: 0 1026 1672 2985 3126 Size: 1026 646 1313 141 Fragments arranged by size: 1313 1026 646 141 Bsp1286I G_dGCh'C Cuts at: 0 92 185 435 990 2516 2991 2997 3126 Size: 92 93 250 555 1526 475 6 129 Fragments arranged by size: 1526 555 475 250 129 93 92 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 165 372 2792 3126 Size: 165 207 2420 334 Fragments arranged by size: 2420 334 207 165 BsrDI GCAATG_nn' Cuts at: 0 472 3126 Size: 472 2654 BsrFI r'CCGG_y Cuts at: 0 215 3126 Size: 215 2911 BstAPI GCAn_nnn'nTGC Cuts at: 0 42 189 3126 Size: 42 147 2937 Fragments arranged by size: 2937 147 42 BstBI TT'CG_AA Cuts at: 0 516 3126 Size: 516 2610 BstXI CCAn_nnnn'nTGG Cuts at: 0 1284 3126 Size: 1284 1842 BstYI r'GATC_y Cuts at: 0 8 363 774 1176 1622 1842 1896 3126 Size: 8 355 411 402 446 220 54 1230 Fragments arranged by size: 1230 446 411 402 355 220 54 8 BstZ17I GTA'TAC Cuts at: 0 1918 3126 Size: 1918 1208 BtgI C'CryG_G Cuts at: 0 1543 3126 Size: 1543 1583 BtsI GCAGTG_nn' Cuts at: 0 115 1677 2353 3126 Size: 115 1562 676 773 Fragments arranged by size: 1562 773 676 115 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2348 2383 3126 Size: 2348 35 743 Fragments arranged by size: 2348 743 35 DraI TTT'AAA Cuts at: 0 1427 3126 Size: 1427 1699 DraIII CAC_nnn'GTG Cuts at: 0 189 3126 Size: 189 2937 EaeI y'GGCC_r Cuts at: 0 217 838 1394 3126 Size: 217 621 556 1732 Fragments arranged by size: 1732 621 556 217 EarI CTCTTCn'nnn_ Cuts at: 0 678 740 1223 1875 2406 2613 3126 Size: 678 62 483 652 531 207 513 Fragments arranged by size: 678 652 531 513 483 207 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 100 3126 Size: 100 3026 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 169 214 352 721 1016 1216 1943 Size: 34 135 45 138 369 295 200 727 Cuts at: 1943 2132 2165 2462 3126 Size: 189 33 297 664 Fragments arranged by size: 727 664 369 297 295 200 189 138 135 45 34 33 EcoNI CCTnn'n_nnAGG Cuts at: 0 268 379 3126 Size: 268 111 2747 Fragments arranged by size: 2747 268 111 EcoO109I rG'GnC_Cy Cuts at: 0 18 159 2994 3126 Size: 18 141 2835 132 Fragments arranged by size: 2835 141 132 18 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 689 2408 3126 Size: 689 1719 718 Fragments arranged by size: 1719 718 689 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 214 246 817 849 1041 1073 1888 1920 Size: 214 32 571 32 192 32 815 32 Cuts at: 1920 2335 2367 2667 2699 3126 Size: 415 32 300 32 427 Fragments arranged by size: 815 571 427 415 300 214 192 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 505 2433 3126 Size: 505 1928 693 Fragments arranged by size: 1928 693 505 HindIII A'AGCT_T Cuts at: 0 2492 3126 Size: 2492 634 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2008 3126 Size: 2008 1118 MscI TGG'CCA Cuts at: 0 840 1396 3126 Size: 840 556 1730 Fragments arranged by size: 1730 840 556 MspA1I CmG'CkG Cuts at: 0 171 510 895 1993 3126 Size: 171 339 385 1098 1133 Fragments arranged by size: 1133 1098 385 339 171 NcoI C'CATG_G Cuts at: 0 1543 3126 Size: 1543 1583 NdeI CA'TA_TG Cuts at: 0 2307 3126 Size: 2307 819 NsiI A_TGCA'T Cuts at: 0 472 687 3126 Size: 472 215 2439 Fragments arranged by size: 2439 472 215 NspI r_CATG'y Cuts at: 0 856 1011 1361 1887 3126 Size: 856 155 350 526 1239 Fragments arranged by size: 1239 856 526 350 155 PciI A'CATG_T Cuts at: 0 1357 1883 3126 Size: 1357 526 1243 Fragments arranged by size: 1357 1243 526 PflMI CCAn_nnn'nTGG Cuts at: 0 192 285 2884 3079 3126 Size: 192 93 2599 195 47 Fragments arranged by size: 2599 195 192 93 47 PfoI T'CCnGG_A Cuts at: 0 11 146 308 3126 Size: 11 135 162 2818 Fragments arranged by size: 2818 162 135 11 PpuMI rG'GwC_Cy Cuts at: 0 159 3126 Size: 159 2967 PsiI TTA'TAA Cuts at: 0 1979 3126 Size: 1979 1147 PspOMI G'GGCC_C Cuts at: 0 2993 3126 Size: 2993 133 PspXI vC'TCGA_Gb Cuts at: 0 2984 3126 Size: 2984 142 PstI C_TGCA'G Cuts at: 0 383 608 1810 3126 Size: 383 225 1202 1316 Fragments arranged by size: 1316 1202 383 225 PvuII CAG'CTG Cuts at: 0 510 895 1993 3126 Size: 510 385 1098 1133 Fragments arranged by size: 1133 1098 510 385 SbfI CC_TGCA'GG Cuts at: 0 383 3126 Size: 383 2743 ScaI AGT'ACT Cuts at: 0 1712 2853 3126 Size: 1712 1141 273 Fragments arranged by size: 1712 1141 273 SexAI A'CCwGG_T Cuts at: 0 185 981 3126 Size: 185 796 2145 Fragments arranged by size: 2145 796 185 SfcI C'TryA_G Cuts at: 0 379 604 701 1806 2423 2914 3126 Size: 379 225 97 1105 617 491 212 Fragments arranged by size: 1105 617 491 379 225 212 97 SmaI CCC'GGG Cuts at: 0 2992 3126 Size: 2992 134 SmlI C'TyrA_G Cuts at: 0 49 2984 3126 Size: 49 2935 142 Fragments arranged by size: 2935 142 49 SrfI GCCC'GGGC Cuts at: 0 2992 3126 Size: 2992 134 SspI AAT'ATT Cuts at: 0 2209 3126 Size: 2209 917 StuI AGG'CCT Cuts at: 0 199 1639 3126 Size: 199 1440 1487 Fragments arranged by size: 1487 1440 199 StyI C'CwwG_G Cuts at: 0 476 1347 1543 3126 Size: 476 871 196 1583 Fragments arranged by size: 1583 871 476 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 469 562 1926 3126 Size: 469 93 1364 1200 Fragments arranged by size: 1364 1200 469 93 TatI w'GTAC_w Cuts at: 0 1108 1122 1710 2599 2851 3126 Size: 1108 14 588 889 252 275 Fragments arranged by size: 1108 889 588 275 252 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 390 422 3126 Size: 390 32 2704 Fragments arranged by size: 2704 390 32 XbaI T'CTAG_A Cuts at: 0 1300 3126 Size: 1300 1826 XhoI C'TCGA_G Cuts at: 0 2984 3126 Size: 2984 142 XmaI C'CCGG_G Cuts at: 0 2990 3126 Size: 2990 136 XmnI GAAnn'nnTTC Cuts at: 0 1190 2391 3126 Size: 1190 1201 735 Fragments arranged by size: 1201 1190 735 Enzymes that do cut: AarI AccI AcuI AflIII AlfI AlwNI ApaI ApoI AseI AvaI AvrII BaeI BamHI BanI BanII BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtsI CspCI DraI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII MmeI MscI MspA1I NcoI NdeI NsiI NspI PciI PflMI PfoI PpuMI PsiI PspOMI PspXI PstI PvuII SbfI ScaI SexAI SfcI SmaI SmlI SrfI SspI StuI StyI TaqII TatI TstI XbaI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaLI AscI AsiSI BbeI BbvCI BcgI BmgBI BmtI BplI BsaAI BsaBI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstEII Bsu36I BtgZI ClaI DrdI EagI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PshAI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SwaI Tth111I XcmI ZraI