##################################
# NO CUT ENZYMES (  213 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MamI        MboII       
MfeI        MflI        MluI        MroI        MseI        
MslI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   18 enzymes) #
###################################

AfaI          225  GTAC                 GT^AC
AlwNI          98  CAGTGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
BalI          189  TGGCCA               TGG^CCA
BciVI           3  GGATAC               GTATCC(6/5)
BseRI          49  CTCCTC               GAGGAG(10/8)
BspHI         198  TCATGA               T^CATGA
BsrI           87  CCAGT                ACTGG(1/-1)
Cac8I          27  GCCTGC               GC[ACGT]^[ACGT]GC
Cfr13I         78  GGCCC                G^G[ACGT]CC
EaeI          189  TGGCCA               [CT]^GGCC[GA]
MaeIII        119  GTAAC                ^GT[ACGT]AC
MluNI         189  TGGCCA               TGG^CCA
MscI          189  TGGCCA               TGG^CCA
NlaIII        199  CATG                 CATG^
RcaI          198  TCATGA               T^CATGA
RsaI          225  GTAC                 GT^AC
Sau96I         78  GGCCC                G^G[ACGT]CC
TspRI          98  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AlwI           73  GGATC                GGATC(4/5)
              195  GGATC               
CfoI           22  GCGC                 GCG^C
               40  GCGC                
DpnII          74  GATC                 ^GATC
              196  GATC                
EcoT14I        58  CCTTGG               C^C[AT][AT]GG
               81  CCAAGG              
HaeII          21  GGCGCT               [GA]GCGC^[CT]
               39  GGCGCT              
HhaI           22  GCGC                 GCG^C
               40  GCGC                
HinP1I         22  GCGC                 G^CGC
               40  GCGC                
MboI           74  GATC                 ^GATC
              196  GATC                
MnlI           51  CCTC                 CCTC(7/6)
               64  CCTC                
NdeII          74  GATC                 ^GATC
              196  GATC                
Sau3AI         74  GATC                 ^GATC
              196  GATC                
StyI           58  CCTTGG               C^C[AT][AT]GG
               81  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AciI        BbvI        BsaJI       BsiYI       BslI        
BssKI       BstNI       EcoRII      Fnu4HI      HaeIII      
ItaI        MvaI        MwoI        ScrFI       TseI