(Circular) (Six-base) MAPSORT of: pFN21AE5143.seq Check: 3414 from: 1 to: 4936 pFN21AE5143 4936 bp With 182 enzymes: SgfI * January 30, 2014 17:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2746 278 Size: 53 83 186 936 1210 2468 Fragments arranged by size: 2468 1210 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2279 1937 Size: 342 4594 Acc65I G'GTAC_C Cuts at: 2257 2257 Size: 4936 AclI AA'CG_TT Cuts at: 1563 3063 3436 1563 Size: 1500 373 3063 Fragments arranged by size: 3063 1500 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2999 4383 4651 1632 Size: 1367 1384 268 1917 Fragments arranged by size: 1917 1384 1367 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4739 AflIII A'CryG_T Cuts at: 1203 3835 4812 1203 Size: 2632 977 1327 Fragments arranged by size: 2632 1327 977 AgeI A'CCGG_T Cuts at: 3767 3767 Size: 4936 AhdI GACnn_n'nnGTC Cuts at: 3665 3665 Size: 4936 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4936 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4936 AlwNI CAG_nnn'CTG Cuts at: 2108 2679 4251 2108 Size: 571 1572 2793 Fragments arranged by size: 2793 1572 571 ApaLI G'TGCA_C Cuts at: 2993 4149 2993 Size: 1156 3780 ApoI r'AATT_y Cuts at: 1493 2245 2471 2623 4732 1493 Size: 752 226 152 2109 1697 Fragments arranged by size: 2109 1697 752 226 152 AseI AT'TA_AT Cuts at: 160 1032 3490 160 Size: 872 2458 1606 Fragments arranged by size: 2458 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4936 AvaI C'yCGr_G Cuts at: 1870 1943 2261 1870 Size: 73 318 4545 Fragments arranged by size: 4545 318 73 BamHI G'GATC_C Cuts at: 2266 2633 2266 Size: 367 4569 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2257 3712 618 Size: 325 217 627 470 1455 1842 Fragments arranged by size: 1842 1455 627 470 325 217 BanII G_rGCy'C Cuts at: 1391 2255 1391 Size: 864 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3965 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3127 3161 1903 Size: 34 1190 34 3678 Fragments arranged by size: 3678 1190 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2002 2828 4038 2002 Size: 826 1210 2900 Fragments arranged by size: 2900 1210 826 BclI T'GATC_A Cuts at: 1548 1548 Size: 4936 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3547 136 Size: 107 122 71 631 605 1875 1525 Fragments arranged by size: 1875 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4931 4931 Size: 4936 BlpI GC'TnA_GC Cuts at: 2353 2353 Size: 4936 Bme1580I G_kGCm'C Cuts at: 1790 2997 4153 1790 Size: 1207 1156 2573 Fragments arranged by size: 2573 1207 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4936 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2667 3625 442 Size: 416 53 83 399 24 1250 958 1753 Fragments arranged by size: 1753 1250 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4936 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3596 1239 Size: 2357 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3061 3926 4224 3061 Size: 865 298 3773 Fragments arranged by size: 3773 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3599 882 Size: 617 2100 2219 Fragments arranged by size: 2219 2100 617 BsaAI yAC'GTr Cuts at: 493 1204 2727 493 Size: 711 1523 2702 Fragments arranged by size: 2702 1523 711 BsaBI GATnn'nnATC Cuts at: 4930 4930 Size: 4936 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2743 3125 3760 275 Size: 53 83 186 936 1210 382 635 1451 Fragments arranged by size: 1451 1210 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3369 3767 4041 4188 4599 3369 Size: 398 274 147 411 3706 Fragments arranged by size: 3706 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4711 Fragments arranged by size: 4711 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 1186 Size: 859 4077 BseYI C'CCAG_C Cuts at: 4139 4635 4139 Size: 496 4440 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4363 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2386 3147 4175 1540 Size: 275 186 385 761 1028 2301 Fragments arranged by size: 2301 1028 761 385 275 186 BsiHKAI G_wGCw'C Cuts at: 2997 3082 4153 2997 Size: 85 1071 3780 Fragments arranged by size: 3780 1071 85 BsmI GAATG_Cn' Cuts at: 2447 2540 2447 Size: 93 4843 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2255 2997 3082 4153 1391 Size: 399 465 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 465 399 85 BspEI T'CCGG_A Cuts at: 4599 4599 Size: 4936 BspHI T'CATG_A Cuts at: 2203 2825 4555 2203 Size: 622 1730 2584 Fragments arranged by size: 2584 1730 622 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2291 844 Size: 1447 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2823 1394 Size: 429 1000 3507 Fragments arranged by size: 3507 1000 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3431 3605 65 Size: 1183 2183 174 1396 Fragments arranged by size: 2183 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3580 3767 1700 Size: 110 1770 187 2869 Fragments arranged by size: 2869 1770 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4936 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4936 BssSI C'ACGA_G Cuts at: 2996 4008 2996 Size: 1012 3924 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4936 BstBI TT'CG_AA Cuts at: 3740 3740 Size: 4936 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4936 BstYI r'GATC_y Cuts at: 1972 2266 2633 3018 3035 4487 4573 4585 4931 Size: 294 367 385 17 1452 86 12 346 Cuts at: 4931 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 294 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3445 Fragments arranged by size: 3445 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4197 Fragments arranged by size: 4197 683 56 BtsI GCAGTG_nn' Cuts at: 2109 2452 3246 3266 2109 Size: 343 794 20 3779 Fragments arranged by size: 3779 794 343 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4936 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4901 DraI TTT'AAA Cuts at: 2240 2240 Size: 4936 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3943 809 Size: 507 2627 1802 Fragments arranged by size: 2627 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2194 2383 3272 Size: 54 1425 50 275 18 364 189 889 Cuts at: 3272 4901 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 364 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2383 1537 Size: 275 571 4090 Fragments arranged by size: 4090 571 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2866 1353 Size: 1513 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3523 3897 4043 387 Size: 1064 138 1934 374 146 1280 Fragments arranged by size: 1934 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2999 3596 4383 4651 1239 Size: 393 1367 597 787 268 1524 Fragments arranged by size: 1524 1367 787 597 393 268 EcoRI G'AATT_C Cuts at: 2245 2623 2245 Size: 378 4558 FspI TGC'GCA Cuts at: 3442 3442 Size: 4936 HaeII r_GCGC'y Cuts at: 1678 2030 2048 4083 1678 Size: 352 18 2035 2531 Fragments arranged by size: 2531 2035 352 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3577 3609 3651 3683 1373 Size: 32 135 32 2005 32 42 32 2626 Fragments arranged by size: 2626 2005 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2280 2526 669 Size: 988 281 342 246 3079 Fragments arranged by size: 3079 988 342 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4936 HpaI GTT'AAC Cuts at: 2526 2526 Size: 4936 KpnI G_GTAC'C Cuts at: 2261 2261 Size: 4936 MfeI C'AATT_G Cuts at: 2535 2535 Size: 4936 MluI A'CGCG_T Cuts at: 4812 4812 Size: 4936 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4050 4234 1305 Size: 2745 184 2007 Fragments arranged by size: 2745 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2196 10 Size: 54 1425 343 364 2750 Fragments arranged by size: 2750 1425 364 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2894 3253 3412 518 Size: 648 1728 359 159 2042 Fragments arranged by size: 2042 1728 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2352 3029 4177 4422 1606 Size: 746 677 1148 245 2120 Fragments arranged by size: 2120 1148 746 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3445 Fragments arranged by size: 3445 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4936 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4936 NotI GC'GGCC_GC Cuts at: 2383 2383 Size: 4936 NspI r_CATG'y Cuts at: 1132 1229 2294 3839 1132 Size: 97 1065 1545 2229 Fragments arranged by size: 2229 1545 1065 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4936 PciI A'CATG_T Cuts at: 3835 3835 Size: 4936 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4936 PmeI GTTT'AAAC Cuts at: 2240 2240 Size: 4936 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3005 3037 4518 4550 3005 Size: 32 1481 32 3391 Fragments arranged by size: 3391 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4936 PsiI TTA'TAA Cuts at: 2506 2506 Size: 4936 PstI C_TGCA'G Cuts at: 830 2288 830 Size: 1458 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4936 SalI G'TCGA_C Cuts at: 1936 2278 1936 Size: 342 4594 SbfI CC_TGCA'GG Cuts at: 2288 2288 Size: 4936 ScaI AGT'ACT Cuts at: 3184 4896 3184 Size: 1712 3224 SfcI C'TryA_G Cuts at: 826 1045 2284 3419 4100 4291 826 Size: 219 1239 1135 681 191 1471 Fragments arranged by size: 1471 1239 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4936 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4936 SmaI CCC'GGG Cuts at: 1872 2263 1872 Size: 391 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3040 3941 4203 820 Size: 197 926 1097 901 262 1553 Fragments arranged by size: 1553 1097 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2727 493 Size: 2234 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4936 SphI G_CATG'C Cuts at: 1132 2294 1132 Size: 1162 3774 SspI AAT'ATT Cuts at: 5 52 2860 5 Size: 47 2808 2081 Fragments arranged by size: 2808 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4936 StyI C'CwwG_G Cuts at: 513 1065 2004 2063 2086 2375 513 Size: 552 939 59 23 289 3074 Fragments arranged by size: 3074 939 552 289 59 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2963 2980 3133 3318 3763 325 Size: 1255 1383 17 153 185 445 1498 Fragments arranged by size: 1498 1383 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2229 3182 4894 Size: 274 80 33 51 1443 252 953 1712 Cuts at: 4894 96 Size: 138 Fragments arranged by size: 1712 1443 953 274 252 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4896 4928 4896 Size: 32 4904 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4936 XbaI T'CTAG_A Cuts at: 2272 2272 Size: 4936 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4936 XmaI C'CCGG_G Cuts at: 1870 2261 1870 Size: 391 4545 XmnI GAAnn'nnTTC Cuts at: 3065 3065 Size: 4936 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2744 276 Size: 53 83 186 936 1210 2468 Fragments arranged by size: 2468 1210 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI