################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MamI MflI MluI MroI MseI MspA1I MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AflIII 176 ACATGT A^C[GA][CT]GT AvaI 397 CTCGGG C^[CT]CG[GA]G BalI 2 TGGCCA TGG^CCA BsmAI 491 GAGAC GTCTC(1/5) BsmI 121 GCATTC GAATGC(1/-1) BsoBI 397 CTCGGG C^[CT]CG[GA]G BspLU11I 176 ACATGT A^CATGT BsrI 349 ACTGG ACTGG(1/-1) BstF5I 267 CATCC GGATG(2/0) DpnII 117 GATC ^GATC DsaI 74 CCGTGG C^C[GA][CT]GG EaeI 2 TGGCCA [CT]^GGCC[GA] EarI 157 CTCTTC CTCTTC(1/4) FauI 195 CCCGC CCCGC(4/6) FokI 267 CATCC GGATG(9/13) HinfI 240 GAGTC G^A[ACGT]TC Ksp632I 157 CTCTTC CTCTTC(1/4) MaeII 388 ACGT A^CGT MaeIII 242 GTCAC ^GT[ACGT]AC MboI 117 GATC ^GATC MfeI 505 CAATTG C^AATTG MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MslI 69 CATTCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 505 CAATTG C^AATTG NdeII 117 GATC ^GATC PleI 240 GAGTC GAGTC(4/5) SapI 156 GCTCTTC GCTCTTC(1/4) Sau3AI 117 GATC ^GATC SfaNI 266 GCATC GCATC(5/9) SfcI 33 CTACAG C^T[GA][CT]AG Tsp45I 242 GTCAC ^GT[GC]AC Tsp509I 506 AATT ^AATT TspRI 329 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AspHI 324 GAGCTC G[AT]GC[AT]^C 378 GAGCTC BanII 324 GAGCTC G[GA]GC[CT]^C 378 GAGCTC BbvI 139 GCTGC GCAGC(8/12) 153 GCTGC BmyI 324 GAGCTC G[AGT]GC[ACT]^C 378 GAGCTC BpmI 237 CTGGAG CTGGAG(16/14) 488 CTGGAG BseRI 381 CTCCTC GAGGAG(10/8) 424 CTCCTC BsiHKAI 324 GAGCTC G[AT]GC[AT]^C 378 GAGCTC BsmFI 10 GGGAC GGGAC(10/14) 452 GTCCC Bsp1286I 324 GAGCTC G[AGT]GC[ACT]^C 378 GAGCTC DdeI 317 CTCAG C^T[ACGT]AG 327 CTCAG DraII 450 GGGTCCC [GA]G^G[ACGT]CC[CT] 496 AGGACCT EcoO109I 450 GGGTCCC [GA]G^G[ACGT]CC[CT] 496 AGGACCT MboII 84 GAAGA GAAGA(8/7) 158 TCTTC NlaIII 177 CATG CATG^ 230 CATG NspI 176 ACATGT [GA]CATG^[CT] 229 ACATGC PpuMI 450 GGGTCCC [GA]G^G[AT]CC[CT] 496 AGGACCT SacI 324 GAGCTC GAGCT^C 378 GAGCTC TseI 139 GCTGC G^C[AT]GC 153 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AciI AluI AvaII BsaJI BsiYI BslI BssKI BstNI Cac8I Cfr13I EcoRII Fnu4HI HaeIII ItaI MnlI MvaI MwoI NlaIV Sau96I ScrFI