Restriction map for
pFN21AE6162
(Circular) (Six-base) MAPSORT of: pFN21AE6162.seq Check: 9207 from: 1 to: 5221
pFN21AE6162 5221 bp
With 182 enzymes: SgfI *
December 8, 2014 13:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3031 278
Size: 53 83 186 936 1495 2468
Fragments arranged by size:
2468 1495 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2564 1937
Size: 627 4594
Acc65I G'GTAC_C
Cuts at: 2542 2542
Size: 5221
AclI AA'CG_TT
Cuts at: 1563 3348 3721 1563
Size: 1785 373 3063
Fragments arranged by size:
3063 1785 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3284 4668 4936 1632
Size: 1652 1384 268 1917
Fragments arranged by size:
1917 1652 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5024
AflIII A'CryG_T
Cuts at: 1203 2181 4120 5097 1203
Size: 978 1939 977 1327
Fragments arranged by size:
1939 1327 978 977
AgeI A'CCGG_T
Cuts at: 4052 4052
Size: 5221
AhdI GACnn_n'nnGTC
Cuts at: 3950 3950
Size: 5221
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5221
AlwNI CAG_nnn'CTG
Cuts at: 2964 4536 2964
Size: 1572 3649
ApaLI G'TGCA_C
Cuts at: 3278 4434 3278
Size: 1156 4065
ApoI r'AATT_y
Cuts at: 1493 2530 2756 2908 5017 1493
Size: 1037 226 152 2109 1697
Fragments arranged by size:
2109 1697 1037 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3775 160
Size: 872 2743 1606
Fragments arranged by size:
2743 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5221
AvaI C'yCGr_G
Cuts at: 1870 1943 2402 2546 1870
Size: 73 459 144 4545
Fragments arranged by size:
4545 459 144 73
BamHI G'GATC_C
Cuts at: 2551 2918 2551
Size: 367 4854
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2542 3997 618
Size: 325 217 627 755 1455 1842
Fragments arranged by size:
1842 1455 755 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2333 2387 2540 1391
Size: 942 54 153 4072
Fragments arranged by size:
4072 942 153 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4250
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3412 3446 1903
Size: 34 1475 34 3678
Fragments arranged by size:
3678 1475 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3113 4323 3113
Size: 1210 4011
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5221
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3832 136
Size: 107 122 71 631 605 2160 1525
Fragments arranged by size:
2160 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5216 5216
Size: 5221
BlpI GC'TnA_GC
Cuts at: 2638 2638
Size: 5221
Bme1580I G_kGCm'C
Cuts at: 1790 3282 4438 1790
Size: 1492 1156 2573
Fragments arranged by size:
2573 1492 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5221
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2952 3910 442
Size: 416 53 83 399 24 1535 958 1753
Fragments arranged by size:
1753 1535 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5221
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 5221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2263 2514 3881 1239
Size: 1024 251 1367 2579
Fragments arranged by size:
2579 1367 1024 251
Bpu10I CC'TnA_GC
Cuts at: 2322 2322
Size: 5221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3346 4211 4509 3346
Size: 865 298 4058
Fragments arranged by size:
4058 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3884 882
Size: 617 2385 2219
Fragments arranged by size:
2385 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3012 493
Size: 711 1808 2702
Fragments arranged by size:
2702 1808 711
BsaBI GATnn'nnATC
Cuts at: 5215 5215
Size: 5221
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3028 3410 4045 275
Size: 53 83 186 936 1495 382 635 1451
Fragments arranged by size:
1495 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3654 4052 4326 4473 4884 3654
Size: 398 274 147 411 3991
Fragments arranged by size:
3991 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4996
Fragments arranged by size:
4996 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2377 2420 1186
Size: 1191 43 3987
Fragments arranged by size:
3987 1191 43
BseYI C'CCAG_C
Cuts at: 2477 4424 4920 2477
Size: 1947 496 2778
Fragments arranged by size:
2778 1947 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4648
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2671 3432 4460 1540
Size: 275 186 670 761 1028 2301
Fragments arranged by size:
2301 1028 761 670 275 186
BsiHKAI G_wGCw'C
Cuts at: 2333 2387 3282 3367 4438 2333
Size: 54 895 85 1071 3116
Fragments arranged by size:
3116 1071 895 85 54
BsmI GAATG_Cn'
Cuts at: 2126 2732 2825 2126
Size: 606 93 4522
Fragments arranged by size:
4522 606 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2333 2387 2540 3282 3367 4438 1391
Size: 399 543 54 153 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 543 399 153 85 54
BspEI T'CCGG_A
Cuts at: 4884 4884
Size: 5221
BspHI T'CATG_A
Cuts at: 3110 4840 3110
Size: 1730 3491
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2576 844
Size: 1732 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3108 1394
Size: 429 1285 3507
Fragments arranged by size:
3507 1285 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3716 3890 65
Size: 1183 2468 174 1396
Fragments arranged by size:
2468 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3865 4052 1700
Size: 110 2055 187 2869
Fragments arranged by size:
2869 2055 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5221
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5221
BssSI C'ACGA_G
Cuts at: 3281 4293 3281
Size: 1012 4209
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5221
BstBI TT'CG_AA
Cuts at: 4025 4025
Size: 5221
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5221
BstYI r'GATC_y
Cuts at: 1972 2551 2918 3303 3320 4772 4858 4870 5216
Size: 579 367 385 17 1452 86 12 346
Cuts at: 5216 1972
Size: 1977
Fragments arranged by size:
1977 1452 579 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2079 513
Size: 552 939 75 3655
Fragments arranged by size:
3655 939 552 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4482
Fragments arranged by size:
4482 683 56
BtsI GCAGTG_nn'
Cuts at: 2737 3531 3551 2737
Size: 794 20 4407
Fragments arranged by size:
4407 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5221
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5186
DraI TTT'AAA
Cuts at: 2525 2525
Size: 5221
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4228 809
Size: 507 2912 1802
Fragments arranged by size:
2912 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2668 3557
Size: 54 1425 50 275 18 177 661 889
Cuts at: 3557 5186 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 661 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2668 1537
Size: 275 856 4090
Fragments arranged by size:
4090 856 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2168 3151 1353
Size: 815 983 3423
Fragments arranged by size:
3423 983 815
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2182 3808 4182 4328 387
Size: 1064 138 593 1626 374 146 1280
Fragments arranged by size:
1626 1280 1064 593 374 146 138
EcoICRI GAG'CTC
Cuts at: 2331 2385 2331
Size: 54 5167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2263 2514 3284 3881 4668 4936 1239
Size: 393 631 251 770 597 787 268 1524
Fragments arranged by size:
1524 787 770 631 597 393 268 251
EcoO109I rG'GnC_Cy
Cuts at: 2456 2502 2456
Size: 46 5175
EcoRI G'AATT_C
Cuts at: 2530 2908 2530
Size: 378 4843
FspI TGC'GCA
Cuts at: 3727 3727
Size: 5221
HaeII r_GCGC'y
Cuts at: 1678 4368 1678
Size: 2690 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2489 2521 3862 3894 3936
Size: 32 135 32 917 32 1341 32 42
Cuts at: 3936 3968 1373
Size: 32 2626
Fragments arranged by size:
2626 1341 917 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2565 2811 669
Size: 988 281 627 246 3079
Fragments arranged by size:
3079 988 627 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5221
HpaI GTT'AAC
Cuts at: 2811 2811
Size: 5221
KpnI G_GTAC'C
Cuts at: 2546 2546
Size: 5221
MfeI C'AATT_G
Cuts at: 2510 2820 2510
Size: 310 4911
MluI A'CGCG_T
Cuts at: 5097 5097
Size: 5221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4335 4519 1305
Size: 3030 184 2007
Fragments arranged by size:
3030 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3222
Fragments arranged by size:
3222 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 3179 3538 3697 518
Size: 648 912 1101 359 159 2042
Fragments arranged by size:
2042 1101 912 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2637 3314 4462 4707 1606
Size: 1031 677 1148 245 2120
Fragments arranged by size:
2120 1148 1031 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3730
Fragments arranged by size:
3730 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5221
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5221
NotI GC'GGCC_GC
Cuts at: 2668 2668
Size: 5221
NspI r_CATG'y
Cuts at: 1132 1229 2185 2238 2579 4124 1132
Size: 97 956 53 341 1545 2229
Fragments arranged by size:
2229 1545 956 341 97 53
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5221
PciI A'CATG_T
Cuts at: 2181 4120 2181
Size: 1939 3282
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5221
PmeI GTTT'AAAC
Cuts at: 2525 2525
Size: 5221
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2430 2462 3290 3322 4803 4835 2430
Size: 32 828 32 1481 32 2816
Fragments arranged by size:
2816 1481 828 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2456 2502 2456
Size: 46 5175
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5221
PsiI TTA'TAA
Cuts at: 2791 2791
Size: 5221
PstI C_TGCA'G
Cuts at: 830 2573 830
Size: 1743 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5221
SacI G_AGCT'C
Cuts at: 2333 2387 2333
Size: 54 5167
SalI G'TCGA_C
Cuts at: 1936 2563 1936
Size: 627 4594
SanDI GG'GwC_CC
Cuts at: 2456 2456
Size: 5221
SapI GCTCTTCn'nnn_
Cuts at: 2168 2168
Size: 5221
SbfI CC_TGCA'GG
Cuts at: 2573 2573
Size: 5221
ScaI AGT'ACT
Cuts at: 3469 5181 3469
Size: 1712 3509
SfcI C'TryA_G
Cuts at: 826 1045 2038 2569 3704 4385 4576 826
Size: 219 993 531 1135 681 191 1471
Fragments arranged by size:
1471 1135 993 681 531 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5221
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5221
SmaI CCC'GGG
Cuts at: 1872 2548 1872
Size: 676 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3325 4226 4488 820
Size: 197 926 1382 901 262 1553
Fragments arranged by size:
1553 1382 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3012 493
Size: 2519 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5221
SphI G_CATG'C
Cuts at: 1132 2579 1132
Size: 1447 3774
SspI AAT'ATT
Cuts at: 5 52 3145 5
Size: 47 3093 2081
Fragments arranged by size:
3093 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5221
StyI C'CwwG_G
Cuts at: 513 1065 2004 2660 513
Size: 552 939 656 3074
Fragments arranged by size:
3074 939 656 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3248 3265 3418 3603 4048 325
Size: 1255 1668 17 153 185 445 1498
Fragments arranged by size:
1668 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3467 5179 96
Size: 274 80 33 51 1443 1490 1712 138
Fragments arranged by size:
1712 1490 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2096 2128 5181 5213 2096
Size: 32 3053 32 2104
Fragments arranged by size:
3053 2104 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5221
XbaI T'CTAG_A
Cuts at: 2557 2557
Size: 5221
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5221
XmaI C'CCGG_G
Cuts at: 1870 2546 1870
Size: 676 4545
XmnI GAAnn'nnTTC
Cuts at: 3350 3350
Size: 5221
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3029 276
Size: 53 83 186 936 1495 2468
Fragments arranged by size:
2468 1495 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI
SwaI XcmI